Redox properties of an H-subunit-depleted photosynthetic reaction center from Rhodopseudomonas viridis.
Biochim Biophys Acta
; 1363(3): 199-208, 1998 Mar 25.
Article
em En
| MEDLINE
| ID: mdl-9518612
ABSTRACT
Recently, we reported that a H-subunit-depleted photosynthetic reaction center (RC-H) was purified from purple nonsulfer photosynthetic bacterium Rhodopseudomonas viridis (Rps. viridis) using a strong detergent sodium alkyl ether sulfate. We compared the redox properties of a native photosynthetic reaction center (RC) and RC-H of Rps. viridis. In RC-H prepared by our method, secondary quinone (QB) was removed while primary quinone (QA) was retained. Absorption spectrum of RC-H was similar to that of RC. After reconstitution of ubiquinone 10 into QB sites, RC-H showed electron transfer activity that was the same as that for native RC. This is the first report about the redox properties of RC-H of Rps. viridis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Rodopseudomonas
/
Complexo de Proteínas do Centro de Reação Fotossintética
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Japão