Binding kinetics and bioassay of RRE mRNA fragments to a peptide containing the recognition domain of HIV-1 Rev.
Biomed Pept Proteins Nucleic Acids
; 2(3): 85-8, 1996.
Article
em En
| MEDLINE
| ID: mdl-9575345
ABSTRACT
Surface plasmon resonance techniques have been used to examine the kinetics of binding for two RNA fragments to an RNA binding domain of HIV-1 REv. RBE3 RNA elicited an apparent dissociation constant (KD) of 121 nM while RREIIB41-79 RNA exhibited an apparent dissociation constant (KD) of 2.5 nM. The dissociation rates for both RNA fragments were comparable. However, the shorter sequence, RBE3, exhibited considerably slower association kinetics. A series of known inhibitors were assayed against these RNA' and the derived K1's were consistent with those reported in the literature, validating the method for routine inhibitor assays.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
/
RNA Viral
/
Produtos do Gene rev
/
Genes env
/
HIV-1
Idioma:
En
Revista:
Biomed Pept Proteins Nucleic Acids
Assunto da revista:
BIOQUIMICA
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Austrália