Broadband ellipso-polarimetric camera utilizing tunable liquid crystal achromatic waveplate with improved field of view.

An ellipso-polarimetric camera integrated with improved field of view tunable achromatic waveplate (AWP) over wide spectral band based on nematic liquid crystal retarders is presented. The AWP operates as half, quarter and full waveplate over a wide range of 430-780nm and wide field of view. The proposed analysis proved that capturing images at these modes is sufficient to extract the ellipsometric parameters: sin(2ψ), cos(Δ) and the Stokes parameters S1 and S3, besides showing the relations in between. Transmission and reflection modes setups are demonstrated in addition to an ellipso-polarimetric smartphone camera. The results show for the first time superiority of cos(Δ) images in which prominent contrast and fine details appear even with scattering objects and higher immunity to device errors. Biometric, remote sensing and archeological improved imaging applications are demonstrated.

Real-Time Ellipsometric Surface Plasmon Resonance Sensor Using Polarization Camera May Provide the Ultimate Detection Limit.

Ellipsometric Surface Plasmon Resonance (SPR) sensors are known for their relatively simple optical configuration compared to interferometric and optical heterodyne phase interrogation techniques. However, most of the previously explored ellipsometric SPR sensors based on intensity measurements are limited by their real-time applications because phase or polarization shifts are conducted serially. Here we present an ellipsometric SPR sensor based on a Kretschmann-Raether (KR) diverging beam configuration and a pixelated microgrid polarization camera. The proposed methodology has the advantage of real-time and higher precision sensing applications. The short-term stability of the measurement using the ellipsometric parameters tanψ and cos(Δ) is found to be superior over direct SPR or intensity measurements, particularly with fluctuating sources such as laser diodes. Refractive index and dynamic change measurements in real-time are presented together with Bovine Serum Albumin (BSA)-anti-BSA antibody binding to demonstrate the potential of the developed sensor for biological sensing applications with a resolution of sub-nM and down to pM with additional optimization. The analysis shows that this approach may provide the ultimate detection limit for SPR sensors.

Biofilm growth monitoring using guided wave ultralong-range Surface Plasmon Resonance: A proof of concept.

Unwelcomed biofilms are problematic in food industries, surgical devices, marine applications, and wastewater treatment plants, essentially everywhere where there is moisture. Very recently, label-free advanced sensors such as localized and extended surface plasmon resonance (SPR) have been explored as tools for monitoring biofilm formation. However, conventional noble metal SPR substrates suffer from low penetration depth (100-300 nm) into the dielectric medium above the surface, preventing the reliable detection of large entities of single or multi-layered cell assemblies like biofilms which can grow up to a few micrometers or more. In this study, we propose using a plasmonic insulator-metal-insulator (IMI) structure (SiO2-Ag-SiO2) with a higher penetration depth based on a diverging beam single wavelength format of Kretschmann configuration in a portable SPR device. An SPR line detection algorithm for locating the reflectance minimum of the device helps to view changes in refractive index and accumulation of the biofilm in real-time down to 10-7 RIU precision. The optimized IMI structure exhibits strong penetration dependence on wavelength and incidence angle. Within the plasmonic resonance, different angles penetrate different depths, showing a maximum near the critical angle. At the wavelength of 635 nm, a high penetration depth of more than 4 µm was obtained. Compared to a thin gold film substrate, for which the penetration depth is only ∼200 nm, the IMI substrate provides more reliable results. The average thickness of the biofilm after 24 h of growth was found to be between 6 and 7 µm with ∼63% live cell volume, as estimated from confocal microscopic images using an image processing tool. To explain this saturation thickness, a graded index biofilm structure is proposed in which the refractive index decreases with the distance from the interface. Furthermore, when plasma-assisted degeneration of biofilms was studied in a semi-real-time format, there was almost no effect on the IMI substrate compared to the gold substrate. The growth rate over the SiO2 surface was higher than on gold, possibly due to differences between surface charge effects. On the gold, the excited plasmon generates an oscillating cloud of electrons, while for the SiO2 case, this does not happen. This methodology can be utilized to detect and characterize biofilms with better signal reliability with respect to concentration and size dependence.

Helical Nanostructures of Ferroelectric Liquid Crystals as Fast Phase Retarders for Spectral Information Extraction Devices: A Comparison with the Nematic Liquid Crystal Phase Retarders.

Extraction of spectral information using liquid crystal (LC) retarders has recently become a topic of great interest because of its importance for creating hyper- and multispectral images in a compact and inexpensive way. However, this method of hyperspectral imaging requires thick LC-layer retarders (50 µm-100 µm and above) to obtain spectral modulation signals for reliable signal reconstruction. This makes the device extremely slow in the case of nematic LCs (NLCs), since the response time of NLCs increases proportionally to the square of the LC-layer thickness, which excludes fast dynamic processes monitoring. In this paper, we explore two approaches for solving the speed problem: the first is based on the use of faster nanospiral ferroelectric liquid crystals as an alternative to NLCs, and the second is based on using a passive multiband filter and focuses on multispectral extraction rather than hyperspectral. A detailed comparative study of nematic and ferroelectric devices is presented. The study is carried out using a 9-spectral bands passive spectral filter, covering the visible and near-infrared ranges. We propose the concept of multispectral rather than hyperspectral extraction, where a small number of wavelengths are sufficient for specific applications.