RESUMEN
Stem cells endowed with skeletogenic potentials seeded in specific scaffolds are considered attractive tissue engineering strategies for treating large bone defects. In the context of craniofacial bone, mesenchymal stromal/stem cells derived from the dental pulp (DPSCs) have demonstrated significant osteogenic properties. Their neural crest embryonic origin further makes them a potential accessible therapeutic tool to repair craniofacial bone. The stem cells' direct involvement in the repair process versus a paracrine effect is however still discussed. To clarify this question, we have followed the fate of fluorescent murine DPSCs derived from PN3 Wnt1-CRE- RosaTomato mouse molar (T-mDPSCs) during the repair process of calvaria bone defects. Two symmetrical critical defects created on each parietal region were filled with (a) dense collagen scaffolds seeded with T-mDPSCs, (b) noncellularized scaffolds, or (c) no scaffold. Mice were imaged over a 3-month period by microcomputed tomography to evaluate the extent of repair and by biphotonic microscopy to track T-mDPSCs. Histological and immunocytochemical analyses were performed in parallel to characterize the nature of the repaired tissue. We show that T-mDPSCs are present up to 3 months postimplantation in the healing defect and that they rapidly differentiate in chondrocyte-like cells expressing all the expected characteristic markers. T-mDPSCs further maturate into hypertrophic chondrocytes and likely signal to host progenitors that form new bone tissue. This demonstrates that implanted T-mDPSCs are able to survive in the defect microenvironment and to participate directly in repair via an endochondral bone ossification-like process. Stem Cells 2019;37:701-711.
Asunto(s)
Regeneración Ósea/genética , Osteogénesis/genética , Cráneo/crecimiento & desarrollo , Proteína Wnt1/genética , Animales , Diferenciación Celular/genética , Condrogénesis/genética , Pulpa Dental/crecimiento & desarrollo , Humanos , Integrasas/genética , Células Madre Mesenquimatosas/citología , Células Madre Mesenquimatosas/metabolismo , Ratones , Células Madre/citología , Ingeniería de TejidosRESUMEN
Modulation of the bio-regenerative characteristics of materials is an indispensable requirement in tissue engineering. Particularly, in bone tissue engineering, the promotion of the osteoconductive phenomenon determines the elemental property of a material be used therapeutically. In addition to the chemical qualities of the constituent materials, the three-dimensional surface structure plays a fundamental role that various methods are expected to modulate in a number of ways, one most promising of which is the use of different types of radiation. In the present manuscript, we demonstrate in a calvarial defect model, that treatment with ultraviolet irradiation allows modification of the osteoconductive characteristics in a biomaterial formed by gelatin and chitosan, together with the inclusion of hydroxyapatite and titanium oxide nanoparticles.
RESUMEN
RESUMEN: En los hospitales públicos chilenos, los pacientes desdentados mandibulares pueden ser tratados con una Sobredentadura-Mucosoportada, Implanto-Retenida (SMIR). Para tal fin, se adaptaron los lineamientos del plan piloto "Programa de Prestaciones Valoradas. Odontología 2012 Ministerio de Salud", en ausencia de una Guía Ministerial. El objetivo de esta serie de casos fue evaluar si la implementación y adaptación del plan piloto del tratamiento con SMIR mejoró la calidad de vida de los pacientes. Se evaluaron sobrevida, éxito implantario, e impacto en la calidad de vida. Los resultados indican una sobrevida y éxito implantario del 100% y una mejora de la calidad de vida. Conclusión: el tratamiento con SMIR mejora la calidad de vida de los pacientes, en el servicio público.
ABSTRACT: In Chilean public hospitals, patients with Complete Edentulous Mandible are treated with Implant-Retained and Mucosa-supported Overdenture (IRMO). For this purpose, the guidelines of the pilot plan "Valued Benefits Program in Dentistry 2012, Ministry of Health" have been adapted. The aim of this study was to evaluate whether these adapted protocols have been successful. We measured implant survival and success, and impact on quality of life with OHIP-14 criteria. Results indicate 100% implant survival and success and improved quality of life. Conclusion: IRMO improves patients' quality of life in public services.
Asunto(s)
Humanos , Masculino , Femenino , Persona de Mediana Edad , Anciano , Calidad de Vida , Servicios de Salud Dental , Prótesis de Recubrimiento , ChileRESUMEN
Neural crest (NC) cells are a migratory, multipotent population giving rise to numerous lineages in the embryo. Their plasticity renders attractive their use in tissue engineering-based therapies, but further knowledge on their in vivo behavior is required before clinical transfer may be envisioned. We here describe the isolation and characterization of a new mouse embryonic stem (ES) line derived from Wnt1-CRE-R26 RosaTomatoTdv blastocyst and show that it displays the characteristics of typical ES cells. Further, these cells can be efficiently directed toward an NC stem cell-like phenotype as attested by concomitant expression of NC marker genes and Tomato fluorescence. As native NC progenitors, they are capable of differentiating toward typical derivative phenotypes and interacting with embryonic tissues to participate in the formation of neo-structures. Their specific fluorescence allows purification and tracking in vivo. This cellular tool should facilitate a better understanding of the mechanisms driving NC fate specification and help identify the key interactions developed within a tissue after in vivo implantation. Altogether, this novel model may provide important knowledge to optimize NC stem cell graft conditions, which are required for efficient tissue repair.