Delivery of Doxorubicin Using Double-Layered Core-Shell Nanocarrier Based on Magnetic Fe3O4 Core and Salep Shells.

Herein, we developed a magnetic drug delivery system based on magnetic Fe3O4 nanoparticles with double shells of modified salep polysaccharide for the delivery of doxorubicin (Dox). The drug-loaded nanocarrier was synthesized in an easy way, and large amounts of drug molecules were loaded into the nanocarrier. The drug-loaded nanocarrier showed excellent pH responsibility in vitro, and large amounts of Dox were released at lower pH (60% release), whereas the nanocarrier was stable at neutral pH. The hemolysis assay results showed that the nanocarrier has negligible hemolytic effects on human red blood cells and showed good biocompatibility. Moreover, the result of coagulation assays showed that the nanocarrier was not active in any coagulation pathways. Cytotoxicity assays of nanocarrier and drug-loaded nanocarrier toward HeLa cells demonstrated that the nanocarrier has negligible toxicity, whereas the drug-loaded nanocarrier kills more than 90% of cells during 48 h. The flow cytometry analysis also showed that the uptake of drug-loaded nanocarrier into the cancerous cells is time-dependent and higher concentrations of drug internalized into the cells at longer incubation time. On the basis of the results, we suggest that the present nanocarrier can be applicable for in vivo drug delivery as an easy-made and cheap nanocarrier.

Magnetite embedded κ-carrageenan-based double network nanocomposite hydrogel with two-way shape memory properties for flexible electronics and magnetic actuators.

Shape memory hydrogels attract increasing attention as flexible strain sensors due to their shape recovery property that can improve the lifetime of the sensor. Herein, we have designed a magnetic shape memory hydrogel based on Fe3O4 nanoparticles, carrageenan, and poly (acrylamide-co-acrylic acid) with self-adhesive and conductive properties. The resulting double network hydrogel showed promising actuator and strain sensor applications. Electrical conductivity was observed in this hydrogel without using additional ions. The presence of magnetite nanoparticles increased the tensile strength and temporary shape fixity ratio to around 6.5 MPa and 94.3 %, respectively. The excellent cantilever and catheter-like behavior of the hydrogels were illustrated through magnetic routing by an external magnet. Also, these hydrogels demonstrated suitable performance in the 500 cycles strain sensing tests before and after their initial shape recovery.

Magnetic nanoparticles double wrapped into cross-linked salep/PEGylated carboxymethyl cellulose; a biocompatible nanocarrier for pH-triggered release of doxorubicin.

A magnetic nanocarrier was synthesized in which Fe3O4 nanoparticles were encapsulated into double layers of polysaccharide shells. The first shell, which was composed of cross-linked salep polysaccharide, contained multiple nitrogen atoms in its structure and provided numerous sites for multiple functionalization. A fluorescence dye and doxorubicin, as widely used chemotherapy agent, were easily attached to the first shell and then a second shell of PEGylated carboxymethyl cellulose enveloped the drug loaded carrier to enhance its biocompatibility and regulates the drug release behavior. The results of drug loading and release behavior showed that the resulting nanocarrier can carry large amounts of drug molecules and a remarkable pH-sensitive release was observed in vitro. The hemolysis and coagulation assays proved the biocompatibility of nanocarrier toward red blood cells and the MTT experiments confirmed that the drug loaded nanocarrier is highly toxic for MCF-7 cancer cells while the unloaded nanocarrier was almost nontoxic. Further flow cytometry experiments and confocal microscopy demonstrated that the double layered magnetic nanocarrier can penetrate into the cells and efficiently release the drug molecules into the cell nucleus. Moreover, the results of MRI experiments performed on the nanocarrier showed that it can be serve as a negative MRI contrast agent.