Evaluation of elements in hair samples of children with developmental language disorder (DLD).

BACKGROUND: Recent studies have highlighted a role for trace elements and toxic metals across neurodevelopmental disorders, including developmental stuttering, Autistic Spectrum Disorders (ASD), and Attention Deficit/Hyperactivity Disorder (ADHD). However, these environmental influences have yet to be explored in relation to Developmental Language Disorder (DLD). METHODS: Elemental hair composition of seven elements; zinc (64Zn), magnesium (26Mg), iron (57Fe), potassium (39K), aluminum (27Al), lead (208Pb), and barium (138Ba) were analyzed in hair samples from 35 children affected by DLD and 35 controls with typical language development (TLD) using both inductive coupled plasma optical emission spectroscopy (ICP-OES) and inductive coupled plasma mass spectroscopy (ICP-MS). RESULTS: The concentration of 64Zn was significantly lower in the hair of DLD group compared to the TLD control group. All other elements showed similar levels between cases and controls. This pilot study demonstrates the utility of trace elements and toxic metals screening in relation to language disorders and the use of hair samples in such investigations. CONCLUSION: The finding that zinc levels differed between cases and controls could represent a clinically relevant result and should be replicated in larger sample size across time. A wider battery of related elements will help to better understand the role of trace elements and toxic metals in DLD.

Profiling plasma levels of thiamine and histamine in Jordanian children with autism spectrum disorder (ASD): potential biomarkers for evaluation of ASD therapies and diet.

Background: The current work involved monitoring two biomarkers in the plasma of children with ASD: the cofactor thiamine that is involved in neurotransmitters modulation for acetylcholine, and the compound histamine, which acts as a neuromodulator by regulating the release of other neurotransmitters. This is the first report to highlight the potential utilization of plasma levels of the selected two brain-related biomarkers in children with ASD.Methods: A total of 43 children with ASD of both genders (age 4-12 years) were involved in this study and compared to age and gender-matched control children (n = 42). The diagnosis of ASD was based on the Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition (DSM5), followed by an additional assessment using the Childhood Autism Rating Scale (CARS). All participants were Jordanian children on Mediterranean diet, and had no history of chronic illness or medications. Measurement of thiamine and histamine in plasma was performed using enzyme-linked immunosorbent assay (ELISA).Results: The outcomes revealed that average histamine levels (31.7 ± 18.5 ng/ml) of ASD group were 5.3× higher (p < .001) compared to their control (0.013 ± 0.011 ng/ml; 6.03 ± 4.25 ng/ml), while thiamine (10.78 ± 7.49 ng/ml) levels of ASD group were significantly lower (p < .001) than the control (37.92 ± 26.87 ng/ml; 0.209 ± 0.054 ng/ml).Conclusions: The study is proposing that monitoring of the plasma levels of thiamine and histamine as biomarkers for future evaluation and development of ASD therapies and nutritious diets.

Amino acids profiles of children who stutter compared to their fluent sibling.

Purpose: This study scrutinises the abundances of 9 neurological-related amino acids of the scalp hair of 35 (5 females, 30 males) children who stutter and 30 normally fluent children (age and sex matched).Methods: Samples of hair from children who stutter aged (5-9 years) were collected from Speech Clinic at King Abdullah Hospital. The control subjects were selected from the same families of children who stutter to exclude the effect of nutritional, environmental, and biological factors. Amino Acid Analyser was used to measure the concentrations of amino acids in acid hydrolysed hair samples.Results: results indicated that the concentrations of threonine, tyrosine, and isoleucine (p = 0.001, 0.001, 0.02 respectively) are lower in hair samples of people who stutter compared with samples of normal fluent speakers.Conclusion: The findings of this study could introduce a new treatment protocol including the supplementation of reduced nutritional elements.KEY POINTSConcentrations of amino acids (threonine, isoleucine, and tyrosine) in the hair samples of people who stutter were significantly lower than the concentrations in the hair samples of control group.Concentrations of amino acids (histidine and glutamate) in hair samples of people who stutter were lower than control group with low significant values.The concentrations of amino acids (phenylalanine, serine, glycine, aspartate, glutamate) were not significantly different between hair samples of stuttering group and control group.The findings of the current study will be helpful in designing a new treatment method based on the supplementation of deficient amino acids.

Memory and potential correlates among children in Jordan.

BACKGROUND: Cognitive functioning hugely affects the performance of tasks of different complexity. Memory, one of the most important cognitive skills, allows children to practice and acquire necessary skills and knowledge and interact with the surrounding environment. Therefore, it is crucial to study the factors that influence the memory of children. The main purpose of his study was to investigate different variables related to memory in school aged children (5-9 years, N = 434). METHODS: Parents of the participants provided information about child's daily behavior, child's school academic achievement, work and family income data and demographics. Memory skills in children were assessed by using the Leiter International Performance Scale -Revised. RESULTS: The score of memory increased 2.53 points with upsurge in maternal occupation level, 3.08 points when the child ate breakfast and 4.51 points when the child daily slept nine hours and more. By contrast, increased family income and smoking by father resulted in decreased scores in memory. CONCLUSION: Screening for and understanding of memory and relevant factors are vital for broad understanding of children's capabilities and weaknesses as well as for developing appropriate interventions.

Concordance between admission and discharge diagnosis of a child and adolescent psychiatry inpatient unit.

BACKGROUND: Psychiatry is arguably the most medical practice that is affected by culture. The pediatric literature is sparse with respect to the differences between child psychiatric units in different cultures and different countries. In this study, we aim to investigate the discordance between admission diagnosis and discharge diagnosis for child psychiatric disorders. METHODS: A retrospective analysis was conducted on 206 patients admitted to a university hospital inpatient child and adolescent psychiatry unit in Ontario, Canada. The data extracted from electronic charts were age, gender, DSM-IV-based diagnosis at admission, living arrangement before admission, length of stay (minimum one day), post-discharge diagnosis, and post-discharge outcomes. RESULTS: There was 75% agreement with the discharge diagnosis. We found strong negative and positive associations between conduct disorder at discharge and the prescription of antipsychotics (+), antidepressants (-), and stimulants (-), and there was a strong association between a conduct disorder (CD) diagnosis and medication-free status. The powerful effect size of stimulant medication was specific to the association between a primary diagnosis of ADHD (vs. not-ADHD) and stimulant medication (c2=127.5, df=1, phi=0.79, P<0.0001). CONCLUSIONS: We have found a significant agreement between admission and discharge diagnosis. It is suggested that the inpatient stay helped to refine the formulation and to improve the child's well-being.

Subject-verb agreement and tense in Jordanian Arabic Broca's aphasia.

The present study examined the deficits of people with aphasia in tense (past, present, and future) and agreement (person, number, and gender) in Jordanian Arabic. Forty participants were selected and two tasks were administered for this study using Boston Diagnostic Aphasia Examination (BDAE)-Arabic version. The first task was sentence completion in which sentences have a blank and need to be filled by the participants. The second task was grammaticality judgment, in which participants should determine whether sentences are grammatically correct or not. Results indicated significantly that the Jordanian participants with aphasia violate gender aspect more than person and number in both sentence completion task (p = 0.025) and grammaticality judgment task (p = 0.000), it was also reported that past tense was violated significantly more than present and future in sentence completion task (p = 0.000) as well as grammaticality judgment task (p = 0.012). It is concluded that Arabic speakers with agrammatism were tested with reference to time and agreement and the results showed that they performed as hypothesized, showing a selective deficit for production of inflected forms of past tense.

Heavy metals and trace elements in scalp hair samples of children with severe autism spectrum disorder: A case-control study on Jordanian children.

BACKGROUND: Elemental analysis has been increasingly used for biomonitoring heavy metals and trace elements. METHODS: This study monitored the levels of two heavy metals (Al and Pb), and seven trace elements (Macroelements Mg, K, P and Ca; Microelements Zn, Cu, Fe) in scalp hair of 57 children with severe autism spectrum disorder (ASD) and 50 age-matched controls, using Inductively Coupled Plasma Atomic Emission Spectrophotometry (ICP-AES). RESULTS: Compared to controls, significantly higher levels of Al (p =  0.001), Pb (p =  0.001) and K (p =  0.021), with lower levels of Mg and Zn (p = 0.038) were observed for the ASD group. ASD boys had higher levels of Al (p =  0.001), Pb (p =  0.001) and K (p =  0.017) than control boys, while ASD girls had higher Pb levels (p =  0.005) than control girls. The ASD subgroup exposed to passive smokers had higher levels of Al (p =  0.033) and Pb (p =  0.001, and the ASD subgroup not exposed to passive smoke had higher levels of Al (p =  0.011), Pb (p =  0.001), K (p =  0.003); and lower levels of Mg (p =  0.011) than their controls. Other confounding factors and the correlation between these elements were also investigated. CONCLUSION: This data suggests that exposure to Al and Pb, increase intake of K, and decreased intake of magnesium and zinc, may contribute to ASD etiology.

Mental and physical health-related quality of life and their associated factors among students of a comprehensive allied health institution.

BACKGROUND: Healthcare students are subjected to critical levels of mental and physical stress that might hinder their quality of life. OBJECTIVES: This study comprehensively investigated physical and mental Health-related Quality of Life (HR-QoL) and their associated factors among Allied Health (AH) students of nine academic majors. METHODS: Participants completed anonymous questionnaire included demographics and life style, HR-QoL measured by the 12-item Short-Form Health Survey (SF-12), Depression Anxiety Stress Scale (DASS21), and Nordic Musculoskeletal Questionnaire. SF-12 Physical (PCS) and Mental (MCS) Components Summary scores were compared between gender and between academic majors. Multiple linear regressions were conducted to examine factor associated with PCS and MCS scores. RESULTS: A total of 838 students (77.4% females) participated in the study. The overall PCS was 45.64±7.93 and statistically different between majors (P < 0.001). The Overall MCS score was 39.45±10.86 and statistically greater in males (P < 0.001). PCS scores were significantly associated with anxiety score, GPA, diet self-evaluation, and upper back and hip musculoskeletal pain. MCS scores were significantly associated with weekly clinical training hours, stress score, depression score, gender, university year, GPA, sleep self-evaluation, diet self-evaluation, and neck musculoskeletal pain. CONCLUSIONS: Low levels of mental and physical HR-QoL were observed among AH students and were associated with academic-related, health-related, and lifestyle-related factors. Longitudinal studies are needed to assess effective approaches to improve HR-QoL among AH students.

Phonological and lexical abilities of Jordanian children with autism.

This study examines phonological and lexical abilities of children with autism in Jordan. JISH Articulation Test (JAT) to measure phonological abilities and JISH School Readiness Screening Test to measure lexical abilities were used in the study. Children with ASD (n = 39) aged 4-8 years and 40 children with typical development were selected to participate in this study. The main finding of this study was that language impairment in both phonological and lexical abilities were noted among Jordanian children with ASD. The results also showed that phonological impairment was greater than lexical impairment. It is concluded that the findings of such study will be helpful to guide experts to provide children with ASD with appropriate educational programs and establish a comprehensive and effective treatment protocols that include phonological and lexical exercises to enhance their speech and language abilities.

The linguistic aspects of the speech of Jordanian children who stutter.

BACKGROUND: The influence of linguistic variables on moments of stuttering has been a popular area of research for several years. The purpose of this study is to assess the linguistic factors of the speech of children who stutter. METHODS: Forty-one children who stutter (31 males, 10 females) were selected from 14 schools with age range of 6-13 years. Phonological and morphological variables were measured using spontaneous speech task, whereas syntactic variables were measured using sentence completion task. RESULTS: Results indicated significantly that (a) inflected words (M = 50, SD = 30) are more likely to be stuttered than non-inflected words (M = 32, SD = 18) [P = 0.000], (b) stuttering occurs more on initial position of the word than medial or final position [P = 0.000], (c) higher rate of stuttering is associated with increasing length of the word [P = 0.000], (d) long sentences are more likely to be stuttered than short sentences [P = 0.000], (e) the loci of stuttering is higher in stressed syllables (M = 34, SD = 30) than unstressed syllables (M = 24, SD = 20) [p = 0.002], (f) the occurrence of stuttering is more on consonant sounds (M = 36, SD = 18) than vowel sounds (M = 25, SD = 28) [P = 0.009]. CONCLUSIONS: These results support the relationship between the linguistic factors and the frequency of stuttering which could be taken into consideration in the development of treatment protocols for children who stutter.

The concentrations of bioelements in the hair samples of Jordanian children who stutter.

OBJECTIVES: This study investigates the levels of 15 bioelements (calcium, copper, chromium, sodium, iron, magnesium, manganese, zinc, cobalt, selenium, molybdenum, vanadium, potassium, boron, and lithium) in the hair species of Jordanian stutterer. METHODS: The subjects of the study included 25 cases of stuttering, and 25 normal children (age and sex matched). The severity of stuttering (low, moderate, and severe) were assessed using Stuttering Severity Instrument, Fourth edition (SSI-4). Hair samples of subjects were cut, washed, dried, physically degraded, hydrolyzed, and analyzed by Inductively Coupled Plasma Mass Spectrometry (ICP-MS). RESULTS: Results indicated that the levels of bioelements (Calcium, Copper, Chromium, Magnesium, Manganese, Cobalt, Selenium, Molybdenum, Vanadium, Boron, and Lithium) were significantly lesser in the hair samples of stuttering group than the control group. CONCLUSION: The findings of the current study could support the use of biochemical analyses as diagnostic biomarker for stuttering.

Discrimination and internalised feelings experienced by people who stutter in Jordan.

PURPOSE: This study investigated the internalised feelings and discrimination experienced by people who stutter in Jordan. METHOD: Five adult speakers who stutter were interviewed as a focus group. The participants were asked about their feelings related to stuttering and discrimination. The participants' responses in the focus group and items adapted from the extant literature formed the basis of a 20-item questionnaire. The questionnaire was administered to 20 additional adults who stutter to assess their internalised feelings about stuttering and their perceived rejecting behaviours (discrimination) associated with their impairment. RESULT: Feeling "annoyed" was the item that received the highest percentage of negative internalised feelings, followed by "embarrassed," "shame," "disappointed," "nervous, "sad," "pessimistic," "fearful," "worried" and "lonely." The results of the discrimination experiences indicated that "getting a leadership position" was the item that most people who stutter reported being worried about, followed by "participation in the classroom," "getting a job," "getting married," "being fully paid in their jobs," "teased," "promoted" and "renting a house." CONCLUSION: These findings provide further evidence of the universality that stuttering is more than the core surface features of speech, but also include aspects that exist below the surface such as negative internalised feelings and various discrimination experiences.