Successful minimally invasive intervention in a primary central incisor after root fracture: A case report.

Root fractures vary in severity, extent, and location, according to the physical and mechanical aspects of the accident. Root fractures are rare in primary teeth and they affect dentin, cementum, periodontal ligament and the pulp. This paper reports a case of a two-and-a-half-year-old baby who had a root fracture of the upper right primary central incisor (tooth 51) as a consequence of trauma that was managed with minimally invasive intervention. After clinical and radiographic examinations, the presence of tooth mobility, pain, bleeding and a horizontal apical root fracture was diagnosed on tooth 51. The treatment of choice was splinting for 120 days, which can deliver excellent results regardless of the patient's age. The tooth was preserved without needing endodontic intervention until its physiologic exfoliation and normal eruption of its permanent successor. As sequelae to the fractured tooth 51, there was some coronal color alteration and dystrophic calcification of the root canal. This tooth did not develop ankylosis or mobility during the follow-up period. After 57 months of follow-up, repositioning and splinting were appropriate ways to manage this horizontal root fracture in the apical third of this primary tooth. It allowed the tooth to be preserved in the arch with normal function.

Bone marrow mononuclear cell therapy for patients with cirrhosis: a Phase 1 study.

BACKGROUND: Bone marrow-derived cell therapy has been investigated in patients with severe liver disease. AIMS: To assess the feasibility, safety and cell kinetics of autologous bone marrow-derived mononuclear cells (BMMCs) infusion in cirrhotic patients. METHODS: BMMCs were isolated from autologous bone marrow and 10% of the cells were labelled with (99m)Tc-SnCl2. Whole body scintigraphy (WBS) was performed 3 and 24 h after infusion via the hepatic artery. Liver function and image were followed during 1 year. RESULTS: Eight patients received 2.0-15.0 × 108 cells. Three and 24-h WBS showed mean hepatic radiotracer retentions of 41 and 32% respectively. One case of dissection of the hepatic artery and one case of Tako-tsubo syndrome occurred as early complications. A patient developed a cutaneous immunomediated disorder and another patient developed hepatocellular carcinoma (HCC) 12 months after infusion. A reduction in bilirubin was shown at 1 week while serum albumin increased above baseline up to 1 month after infusion (P<0.05). CONCLUSIONS: BMMCs infusion is feasible and practical in a clinical setting. In vivo tracking of labelled cells demonstrated that the hepatic artery route successfully delivered BMMCs to the liver. The early improvement of laboratory indices of liver function should be interpreted with caution, because this study was not designed to evaluate efficacy. The median Model for End-Stage Liver Disease score had not deteriorated 1 year later. The occurrence of a graft-versus-host disease-like phenomenon highlights the importance of sustained vigilance even when giving autologous cells. Controlled studies are needed to determine whether BMMCs infusion affects HCC development in cirrhosis.

Customization and external validation of the Simplified Mortality Score for the Intensive Care Unit (SMS-ICU) in Brazilian critically ill patients.

PURPOSE: To customize and externally validate the recently proposed Simplified Mortality Score for the ICU (SMS-ICU, a simple score for 90-day mortality that has no need for ancillary testing results) for in-hospital mortality and to compare its performance to SAPS 3. MATERIAL AND METHODS: We used data from two distinct large cohorts of adult Brazilian patients with unplanned ICU admissions to perform a first-level customization (43,017 patients admitted to 78 ICUs) of the original SMS-ICU score for in-hospital mortality and, sequentially, externally validate it (313,365 patients admitted to 99 ICUs). Performance of SMS-ICU was assessed through measurements of discrimination and calibration and compared with SAPS 3. RESULTS: In the validation cohort, median SMS-ICU was 13 (IQR 8-16) points and median SAPS 3 was 44 (IQR 36-51). Discrimination of SMS-ICU was good (AUC 0.817; 95% CI 0.814-0.819) but slightly lower than of SAPS 3 (AUC 0.845; 95% CI 0.843-0.848;). The customized SMS-ICU predictions were comparable to SAPS 3 in terms of calibration. CONCLUSION: In this external validation of the SMS-ICU in a large Brazilian cohort, we observed good discrimination of SMS-ICU and acceptable calibration after first-level customization. SMS-ICU can be used as a measure of illness severity for acutely admitted ICU patients in clinical studies.

Prospective study of potential sources of Streptococcus mutans transmission in nursery school children.

Transmission of Streptococcus mutans, a major dental caries pathogen, occurs mainly during the first 2.5 years of age. Children appear to acquire S. mutans mostly from their mothers, but few studies have investigated non-familial sources of S. mutans transmission. This study prospectively analysed initial S. mutans oral colonization in 119 children from nursery schools during a 1.5-year period and tracked the transmission from child to child, day-care caregiver to child and mother to child. Children were examined at baseline, when they were 5-13 months of age, and at 6-month intervals for determination of oral levels of S. mutans and development of caries lesions. Levels of S. mutans were also determined in caregivers and mothers. A total of 1392 S. mutans isolates (obtained from children, caregivers and mothers) were genotyped by arbitrarily primed PCR and chromosomal RFLP. Overall, 40.3 % of children were detectably colonized during the study, and levels of S. mutans were significantly associated with the development of caries lesions. Identical S. mutans genotypes were found in four nursery cohorts. No familial relationship existed in three of these cohorts, indicating horizontal transmission. Despite high oral levels of S. mutans identified in most of the caregivers, none of their genotypes matched those identified in the respective children. Only 50 % of children with high levels of S. mutans carried genotypes identified in their mothers. The results support previous evidence indicating that non-familial sources of S. mutans transmission exist, and indicate that this bacterium may be transmitted horizontally between children during the initial phases of S. mutans colonization in nursery environments.

The effects of prenatal and postnatal malnutrition on the morphology, differentiation, and metabolism of skeletal striated muscle tissue in rats.

OBJECTIVE: To study the contractile properties, metabolism and morphological characteristics of muscles submitted to prenatal and postnatal protein malnutrition. METHODS: Animals were distributed into two groups: Control, normoprotein diet (CG; n = 15; 5/5/5), and Malnourished, hypoprotein diet (MG; n = 15; 5/5/5), and examined on the 7th, 14th, and 28th days of the experiment. Total body mass, weight, and the contractile properties and morphology of the anterior tibial muscle were assessed. Several 8 microm-thick tissue samples were taken from 7, 14, and 28 day old rats and stained with HE or subjected to NADH-TR or m-ATPase (pH = 4.4) techniques. RESULTS: Body and muscle weight were lower in the malnourished group. On the 7th day of malnutrition, muscle samples exhibited fibers with smaller diameter, higher polymorphism and higher endomysial conjunctive tissue content. Histochemical methods were unable to precisely determine the types of fiber present. On the 14th day, there were smaller muscle fibers, more polymorphism, many of them with central nuclei and moderate endomysial conjunctive tissue content. With reference to contractile properties, the m-ATPase reaction identified both slow and fast fibers. The NADH-TR reaction revealed the following types of fiber: slow oxidative (SO), fast oxidative glycolytic (FOG) and fast glycolytic (FG). On the 28th day smaller, bunched muscle fibers varying shapes. All three types of fiber exhibited unclear recognition limits with respect to contraction and metabolism. CONCLUSION: Our experimental results suggest that, in addition to the reduction in numbers of fibers, malnutrition retards the differentiation of the morphological, metabolic, and contractile characteristics of skeletal muscle fibers in growing rats.

Manifestações clínicas da disfunção temporomandibular em indivíduos com dentição natural e usuários de prótese total dupla / Clinical manifestations of temporomandibular dysfunction in naturally dented and double total prosthesis users / Expresiones clínicas de disfunción temporomandibular en personas y usuarios dentiados naturales de prostesis doble total

Objetivo: Esta pesquisa teve como objetivo comparar as manifestações clínicas da disfunção temporomandibular (DTM) em indivíduos com dentição natural e usuários de prótese total dupla. Métodos: A amostra do estudo consistiu em 120 participantes, distribuídos em dois grupos de 60 indivíduos cada. O Grupo 1 era composto por pessoas com dentição natural, enquanto o Grupo 2 era composto por indivíduos que utilizavam próteses totais duplas. Para identificar os sinais e sintomas da DTM, foram realizados tanto o índice anamnésico quanto o exame físico específico. Resultados: Os indivíduos que utilizavam prótese total apresentaram um aumento de 2,63 vezes no risco de apresentar dor de ouvido e um aumento de 5,42 vezes de ter limitação do movimento lateral da mandíbula. Por outro lado, os indivíduos com dentição natural apresentaram maior sensibilidade à palpação dos músculos temporal anterior e temporal posterior, além de um risco de 0,27 vezes maior de ter sensibilidade articular. Conclusões: Concluiu-se que os principais sinais clínicos de DTM dos indivíduos dentados naturais foram a sensibilidade à palpação muscular e a sensibilidade à palpação articular e no grupo de desdentados a limitação de movimento lateral da mandíbula e o sintoma de dor de ouvido foram mais frequentes.

Objective: This research aimed to compare the clinical manifestations of temporomandibular dysfunction (TMD) in individuals with natural dentition and users of double total prosthesis. Methods: The study sample consisted of 120 participants, distributed in two groups of 60 individuals each. Group 1 was composed of people with natural dentition, while Group 2 was composed of individuals who used double total prostheses. To identify the signs and symptoms of TMD, both the anamnestic index and the specific physical examination were performed. Results: The individuals who used total prosthesis showed an increase of 2.63 times in the risk of presenting ear pain and an increase of 5.42 times of having limitation of the lateral movement of the jaw. On the other hand, individuals with natural dentition showed greater sensitivity to palpation of the anterior and posterior temporal muscles, in addition to a 0.27 times greater risk of joint tenderness. Conclusions: It was concluded that the main clinical signs of TMD of natural dentate individuals were the sensitivity to muscle palpation and the sensitivity to joint palpation and in the group of toothless individuals the limitation of lateral jaw movement and the ear pain symptom were more frequent.

Propósito: El objetivo de esta investigación fue comparar las manifestaciones clínicas de disfunción temporal (DTM) en individuos con dientes naturales y usuarios de prótesis doble total. Métodos: La muestra de estudio consistió en 120 participantes, distribuidos en dos grupos de 60 individuos cada uno. El grupo 1 estaba formado por personas con dientes naturales, mientras que el grupo 2 estaba formado por individuos con prótesis dobles totales. Para identificar los signos y síntomas de la DTM, se realizó tanto el índice anamésico como el examen físico específico. Resultados: los individuos que utilizaron la prótesis total mostraron un incremento de 2,63 veces en el riesgo de sufrir un dolor de oído y un aumento de 5,42 veces en la limitación del movimiento lateral de la mandíbula. Por otro lado, los individuos con dientes naturales mostraron mayor sensibilidad a la palpación de los músculos temporales anteriores y posteriores, además de un riesgo de 0,27 veces mayor de sensibilidad articular. Conclusiones: Se concluyó que los principales signos clínicos de MSD en sujetos con dientes naturales fueron sensibilidad a la palpación muscular y sensibilidad a la palpación articular y al grupo sin dientes, limitación del movimiento lateral de la mandíbula y síntoma de cefalea fueron más frecuentes.

Influência da qualidade das próteses totais na prevalência de disfunção temporomandibular / Influence of quality of total prostheses on the prevalence of temporomandibular disorder

Introduction Temporomandibular disorder (TMD) involves the mastication and temporomandibular articulation muscles. Completely toothless patients with prostheses should be thoroughly assessed since edentulism causes damage that can lead to TMD onset. Objective This study aims to assess the effect of the technical quality of total prostheses (TPs) on TMD occurrence in patients with bimaxillary TPs. Material and method 154 patients responded to a questionnaire to obtain personal data, information regarding prostheses, anamnestic index for TMD, and the prosthesis quality index. Result Of the 154 patients examined, 64 (41.6%) had no TMD, 61 (39.6%) had mild TMD, 23 (14.9%) moderate, and only 6 (3.9%) showed severe TMD. As for the quality of total prosthesis, 78 (50.6%) completely toothless individuals had satisfactory TPs while 76 (49.4) presented unsatisfactory prostheses. Regarding the time using total prosthesis, around 50% of individuals with up to 10 years use presented some degree of TMD and 70% of individuals with over 10 years presented TMD. Conclusion Considering the methodology used and the population studied, we conclude that the quality of the TPs did not influence the incidence of TMD.

Introdução A disfunção temporomandibular (DTM) é uma desordem que envolve os músculos da mastigação e da articulação temporomandibular. Pacientes desdentados totais e que utilizam protéses requerem uma avaliação criteriosa, já que o edentulismo leva a prejuízos que podem contribuir para o aparecimento de DTM. Objetivo O objetivo deste presente estudo foi avaliar o efeito da qualidade técnica das próteses totais (PTs) na incidência de DTM em pacientes portadores de PTs bi-maxilares. Material e método Foi aplicado um questionário a 154 pacientes contendo os dados pessoais, o histórico das próteses, o índice anamnésico para DTM e o índice de qualidade de prótese. Resultado Dos 154 pacientes examinados, 64 (41,6%) estavam livres de DTM, 61 (39,6%) possuíam DTM leve, 23 (14,9%), moderada e apenas 6 (3,9%) mostravam DTM severa. Quanto à qualidade da prótese total, 78 (50,6%) desdentados totais portavam PTs satisfatórias e 76 (49,4) estavam com próteses insatisfatórias. Quanto ao tempo de uso da prótese total, cerca de 50% dos indivíduos com até 10 anos de uso apresentaram algum grau de DTM e 70% dos indivíduos com mais de 10 anos acusaram a presença de DTM. Conclusão Considerando-se a metodologia empregada e a população estudada, pôde-se concluir que a qualidade das PTs não influenciou na presença de DTM.

Confiabilidade interavaliadores e teste-reteste da escala de participação (P-Scale) em pacientes após acidente vascular cerebral / Interexaminer and test-retest reliability of the Participation Scale (P-Scale) in stroke patients

A versão brasileira da Escala de Participação (P-Scale) é um instrumento de avaliação que mensura restrições em participação em indivíduos com diversas condições de saúde.Objetivos: Avaliar a confiabilidade interexaminador e teste-reteste da versão brasileira da P-Scale em um grupo de pacientes pós-acidente vascular cerebral (AVC).Método: Estudo metodológico, avaliou 20 pacientes com diagnóstico de AVC atendidos em um Centro de Reabilitação. A confiabilidade do escore total foi avaliado por meio do Coeficiente de Correlação Intraclasse (ICC). A confiabilidade interavaliador e teste-reteste de cada item foi medida pelo Coeficiente Kappa com ponderação quadrática. Para avaliação da concordância entre os escores obtidos item por item interavaliador e teste-reteste, foi utilizado o gráfico Bland-Altman.Resultados: A versão brasileira da P-Scale apresentou confiabilidade quase perfeita da maioria dos itens (kw>0,81), sem erros sistemáticos de mensuração avaliados pelo gráfico de concordância de Bland-Altman (p= 0,350) e excelente confiabilidade teste-reteste do escore total (ICC= 0,96; p= 0,000). Em relação à confiabilidade interexaminador, os itens apresentaram confiabilidade variando de quase perfeita (kw>0,81) a regular (0,210,40) e o gráfico de concordância de Bland-Altman indicou diferenças sistemáticas de mensuração (p= 0,018) apenas para dois casos atípicos. ICC da confiabilidade interexaminador do escore total foi excelente tanto na análise da amostra total (ICC= 0,95 para 20 indivíduos; p= 0,000), quanto na amostra sem os casos atípicos (ICC= 0,97 para 18 casos; p= 0,000).Conclusão: A versão brasileira da P-Scale apresentou excelente confiabilidade teste-reteste e interexaminador para avaliar participação em pacientes após AVC.

The Brazilian version of the Participation Scale (P-Scale) is an evaluation instrument that assesses the restrictions in participation in individuals with several different health conditions. Objectives:Evaluating the interexaminer and test-retest reliability of the Brazilian verson of the P-Scale in a group of stroke patients. Methods:Methodological study that evaluated 20 stroke patients attended in a Rehabilitation Center. The reliability of the total score was evaluated using the Intraclass Correlation Coefficient (ICC). The interexaminer and test-retest reliability of each item were measured using the quadratic weighted Kappa Coefficient. To evaluate the agreement between the scores obtainted per each interexaminer and test-retest item, the Bland-Altman graphic was used. Results: The Brazilian version of the P-Scale presented an almost perfect reliability in most items (kw>0.81), with no systemic measuring errors according to the Bland-Altman agreement graphic (p=0.350) and an excellent total score test-retest reliability (ICC=0.96; P=000). Regarding interexaminer reliability, the items varied from almost perfect (kw>0.81) to regular (0.210.40) and the Bland-Altman agreement graphic only indicated systemic measuring differences (p=0.018) in two atypical cases. The ICC of interexaminer reliability total score was excellent in both the analysis of the entire sample (ICC=0.95 for 20 individuals; p=0.000) and in the sample without including the atypical cases (ICC=0.97 for 18 individuals; p=0.000). Conclusion:The Brazilian version of the P-Scale presented excellent interexaminer and test-retest reliability to evaluate participation in stroke patients.

External validation of SAPS 3 and MPM0-III scores in 48,816 patients from 72 Brazilian ICUs.

BACKGROUND: The performance of severity-of-illness scores varies in different scenarios and must be validated prior of being used in a specific settings and geographic regions. Moreover, models' calibration may deteriorate overtime and performance of such instruments should be reassessed regularly. Therefore, we aimed at to validate the SAPS 3 in a large contemporary cohort of patients admitted to Brazilian ICUs. In addition, we also compared the performance of the SAPS 3 with the MPM0-III. METHODS: This is a retrospective cohort study in which 48,816 (medical admissions = 67.9%) adult patients are admitted to 72 Brazilian ICUs during 2013. We evaluated models' discrimination using the area under the receiver operating characteristic curve (AUROC). We applied the calibration belt to evaluate the agreement between observed and expected mortality rates (calibration). RESULTS: Mean SAPS 3 score was 44.3 ± 15.4 points. ICU and hospital mortality rates were 11.0 and 16.5%. We estimated predicted mortality using both standard (SE) and Central and South American (CSA) customized equations. Predicted mortality rates were 16.4 ± 19.3% (SAPS 3-SE), 21.7 ± 23.2% (SAPS 3-CSA) and 14.3 ± 14.0% (MPM0-III). Standardized mortality ratios (SMR) obtained for each model were: 1.00 (95% CI, 0.98-0.102) for the SAPS 3-SE, 0.75 (0.74-0.77) for the SAPS 3-CSA and 1.15 (1.13-1.18) for the MPM0-III. Discrimination was better for SAPS 3 models (AUROC = 0.85) than for MPM0-III (AUROC = 0.80) (p < 0.001). We applied the calibration belt to evaluate the agreement between observed and expected mortality rates (calibration): the SAPS 3-CSA overestimated mortality throughout all risk classes while the MPM0-III underestimated it uniformly. The SAPS 3-SE did not show relevant deviations from ideal calibration. CONCLUSIONS: In a large contemporary database, the SAPS 3-SE was accurate in predicting outcomes, supporting its use for performance evaluation and benchmarking in Brazilian ICUs.

Augmented cell survival in eutopic endometrium from women with endometriosis: expression of c-myc, TGF-beta1 and bax genes.

BACKGROUND: Endometriosis is a common gynaecological disorder characterized by the presence of endometrial tissue outside of the uterus. The fragments in normal menstruation are composed of necrotic and living cells, which do not survive in ectopic locations because of programmed cell death. The aim of this study was to evaluate if the balance between cell proliferation and apoptosis is changed in eutopic endometrium from women with endometriosis throughout the menstrual cycle by studying bax (pro-apoptotic), c-myc (regulator of cell cycle) and TGF-beta1 (involved in cell differentiation) genes. METHODS: Eutopic endometrium was obtained from: 30 women with endometriosis (32.8 +/- 5 years) and 34 fertile eumenorrheic women (36 +/- 5.3 years). We analyzed apoptosis (TUNEL: DNA fragmentation); cell proliferation (immunohistochemistry (IHC) for Ki67); c-myc, bax and TGF-beta1 mRNA abundance (RT-PCR) and TGF-beta1 protein (IHC) in endometrial explants. RESULTS: Cell proliferation strongly decreased from proliferative to late secretory phases in glands, but not in stroma, in both endometria. Positive staining in glands and stroma from proliferative endometrium with endometriosis was 1.9- and 2.2-fold higher than control endometrium, respectively (p < 0.05). Abundance of c-myc mRNA was 65% higher in proliferative endometrium from endometriosis than normal tissue (p < 0.05). TGF-beta1 (mRNA and protein) augmented during mid secretory phase in normal endometrium, effect not observed in endometrium with endometriosis. In normal endometrium, the percentage of apoptotic epithelial and stromal cells increased more than 30-fold during late secretory phase. In contrast, in endometrium from endometriosis, not only this increase was not observed, besides bax mRNA decreased 63% versus normal endometrium (p < 0.05). At once, in early secretory phase, apoptotic stromal cells increased 10-fold with a concomitant augment of bax mRNA abundance (42%) in endometria from endometriosis (p < 0.05). CONCLUSION: An altered expression of c-myc, TGF-beta1 and bax was observed in eutopic endometrium from endometriosis, suggesting its participation in the regulation of cell survival in this disease. The augmented cell viability in eutopic endometrium from these patients as a consequence of a reduction in cell death by apoptosis, and also an increase in cell proliferation indicates that this condition may facilitate the invasive feature of the endometrium.

[Maltreatment: study from the perspective of the police department for the protection of the child and the adolescent in Salvador, Bahia]. / Maus-tratos: estudo através da perspectiva da delegacia de proteção à criança e ao adolescente em Salvador, Bahia.

This cross-sectional study describes the occurrences of maltreatment recorded over three years (1997-1999) in a Brazilian Police Department for the Protection of the Child and the Adolescent. Socio-demographic and physical data of victims, aggressors and denouncers were investigated in a sample consisting of 2073 cases. The victims were aged between 0-18 years, of which 56.1% were girls. Data were entered into EPI INFO 2000 software and analyzed using descriptive statistics and chi-square test to determine significant differences (p<0.05). All manifestations of abuse, except physical abuse, tend to occur more frequently in females, and tend to be more prevalent in the 11-to-15 year-old subgroup. Physical abuse was the most common offense (64.7%). Injuries of face and jaw were found in 65.3% of the cases. Most of the aggressors were males (71.8%). There was a positive correlation between gender (female) and sexual abuse. Physical violence against boys was more intensive, as indicated by the presence of more severe body lesions. Health professionals should increase their diagnostic skills and raise the awareness of their communities to take action for reducing the incidence and the impact of violence upon children and adolescents.

Caracterização física, química e bioquímica do mesocarpo interno de frutos do pequizeiro colhidos em diferentes estádios de desenvolvimento / Physical, chemical and biochemical characterization of pequi fruit harvested at different stages of development

O objetivo deste trabalho foi a caracterização física, química e bioquímica do pequi, produzido no sul de Minas Gerais, ao longo do seu desenvolvimento. Frutos coletados na 8ª, 9ª, 10ª, 12ª e 13ª semanas após a antese foram selecionados quanto ao tamanho, cor da casca, ausência de injúrias e avaliados quanto à firmeza da polpa, teores de beta-caroteno, de açúcares solúveis totais e de pectinas total e solúvel, assim como quanto à atividade das enzimas pectinametilesterase (PME), poligalacturonase (PG), peroxidase (POD) e polifenoloxidase (PPO). Verificou-se que, com o avanço dos dias, após a antese nos frutos do pequizeiro, ocorre a síntese do beta-caroteno e de açúcares solúveis totais, redução da firmeza, solubilização das pectinas, ausência de PME, diminuição na atividade da PG e aumento nas atividades da POD e da PPO na polpa.

The aim of this study was to characterize physical, chemical and biochemical pequi fruit produced in southern Minas Gerais, Brazil, during their development stages. Fruits collected in the 8th, 9th, 10th, 12th and 13th weeks after anthesis, were selected for size, skin color, and absence of injuries and evaluated according to firmness, levels of beta-carotene, total soluble sugars, and total and soluble pectins as well as the activity of pectinmethylesterase (PME), polygalacturonase (PG), peroxidase (POD) and polyphenoloxidase (PPO). It was found that with the advancement of days after anthesis occurs in pequi fruits the synthesis of beta-carotene and total soluble sugars, reducing firmness, pectic solubilization, absence of PME activity, decrease in PG activity and increased in activities POD and PPO in the pulp.

Age-specific salivary immunoglobulin A response to Streptococcus mutans GbpB.

In a follow-up study of children infected with Streptococcus mutans at an early age (children previously shown to respond poorly to S. mutans GbpB), there was a delay in their immune response, rather than a complete inability to respond to this antigen. Epitopes in the N-terminal third of GbpB were identified as targets for naturally induced immunoglobulin A antibody in children at an early age.

[Severe imported malaria: case report]. / Malária grave importada: relato de caso.

BACKGROUND AND OBJECTIVES: Malaria is still considered a major global health problem. The severity form of the disease is caused, mainly by P. falciparum and may occur together with cerebral, kidney, pulmonary, hematologic, circulatory and hepatic complications. This report is about a patient with a case of severe imported malaria. CASE REPORT: A 30-years-old man, mulatto, Philippine, sailor, coming from a ship arriving from Nigeria, with a history of abdominal pain on the right hypochondrium, jaundice, fever, decreased in the consciousness. Lab tests made upon his admission showed hyperbilirubinemia at a level of 50 mg/dL, severe metabolic acidosis, thrombocytopenia, creatinine levels of 5.6 mg/dL and leukocytosis with deviation through metamyelocytes. The APACHE II score was 37, with death estimated risk of 88%. During his stay at the hospital, P. Falciparum Malaria was diagnosed through the thick drop test. And, even with the adequate anti-malaria therapy, the patient’s condition evolved to an acute renal failure requiring hemodialis; acute respiratory distress syndrome (ARDS); septic shock, and hematological disorders, forming a multiple organ dysfunction syndrome (MODS). After being discharged from the hospital, the patient did not present any cerebral, pulmonary or kidney sequel. CONCLUSIONS: From the criteria described in medical literature to define critical malaria, the patient fulfilled the following: acute renal failure, ARDS, metabolic acidosis, altered level of consciousness, macroscopic hemoglobinuria, hyperparasitism and hyperbilirubinemia, related to a lethality rate of over 10%, depending on early treatment and available resources. Severe malaria requires fast diagnosis allied to a quick access to an intensive care treatment, since any delay increases the morbid-mortality of the disease.

Characterization of salivary immunoglobulin A responses in children heavily exposed to the oral bacterium Streptococcus mutans: influence of specific antigen recognition in infection.

The initial infection of children by Streptococcus mutans, the main pathogen of dental caries, depends on the ability of S. mutans to adhere and accumulate on tooth surfaces. These processes involve the adhesin antigen I/II (AgI/II), glucosyltransferases (GTF) and glucan-binding protein B (GbpB), each a target for anticaries vaccines. The salivary immunoglobulin A (IgA) antibody responses to S. mutans antigens (Ags) were characterized in 21 pairs of 5- to 13-month-old children. Pairs were constructed with one early S. mutans-infected and one noninfected child matched by age, racial background, number of teeth, and salivary levels of IgA. Specific salivary IgA antibody response and S. mutans infection levels were then measured during a 1-year follow-up. Robust responses to S. mutans were detected from 6 months of age. Salivary IgA antibody to AgI/II and GTF was commonly detected in salivas of all 42 children. However, GbpB-specific IgA antibody was seldom detected in the subset of infected children (38.1% at baseline). In contrast, most of the subset of noninfected children (76.2%) showed GbpB-reactive IgA antibody during the same period. Frequencies of GbpB responses increased with age, but differences in intensities of GbpB-IgA antibody reactions were sustained between the subsets. At baseline, GbpB-reactive IgA antibody accounted for at least half of the total salivary IgA S. mutans-reactive antibody in 33.3 and 9.5% of noninfected and infected children, respectively. This study provides evidence that a robust natural response to S. mutans Ags can be achieved by 1 year of age and that IgA antibody specificities may be critical in modulating initial S. mutans infection.

[P450Arom and estrogenic microenvironment of eutopic endometria in endometriosis]. / P450Arom y microambiente estrogénico en endometrios eutópicos de mujeres con endometriosis.

BACKGROUND: Endometriosis, a common gynecologic disorder characterized by endometrial glands and stroma outside the uterus, is diagnosed by direct visualization of peritoneal and ovarian implants during laparoscopy. AIM: To study the estrogenic microenvironment in eutopic endometria of women with and without endometriosis. PATIENTS AND METHODS: Eutopic endometria, obtained during laparoscopy from 23 women with endometriosis and 20 fertile cyclic women undergoing tubal sterilization, was studied. P450Arom mRNA expression (RT-PCR) was measured. Also, P450Arom activity was assessed measuring testosterone conversion to estradiol and the concentration of this last hormone in cultured endometrial explants. RESULTS: Age and body mass index was similar in both groups studied. Seventy nine percent of endometria from women with endometriosis and in 29.4% from control group expressed P450Arom mRNA (p <0.01). The intensity of the band was higher in secretory endometria from women with endometriosis when compared to controls (p <0.01), but it was similar during the proliferative phase. Estradiol secretion to the culture media by proliferative endometria explants from women with endometriosis was 3-fold higher than secretory endometria (p <0.01) and endometria from control women in both phases. P450Arom activity, in the presence of testosterone, was 7-fold higher in endometrial cultures from women with endometriosis, when compare with the basal culture (p <0.01). However, in endometrial explant cultures from control women, this activity was not statistical different. CONCLUSIONS: These results indicate that in women with endometriosis, the microenvironment in the endometria is estrogenic as a consequence of an increased expression and activity of the P450Arom.

Maus-tratos: estudo através da perspectiva da delegacia de proteção à criança e ao adolescente em Salvador, Bahia / Maltreatment: study from the perspective of the police department for the frotection of the child and the adolescent in Salvador, Bahia

Este estudo transversal descreve a ocorrência de maus-tratos em uma delegacia de proteção a crianças e adolescentes em Salvador, Bahia, entre 1997 e 1999. Dados sociodemográficos e físicos das vítimas, agressores e denunciantes foram investigados a partir de uma amostra de 2.073 casos. As vítimas apresentaram idades entre 0 e 18 anos, 56,1 por cento do sexo feminino. De todas as manifestações de maus-tratos, exceto o abuso físico, prevaleceram as sobre o sexo feminino. O subgrupo de pré-adolescentes foi o mais atingido. O tipo de maltrato mais freqüente foi o abuso físico (64,7 por cento). Lesões corporais ocorreram em 22,2 por cento dos casos, a maioria concentrando-se na cabeça e pescoço (65,3 por cento). Observou-se intensa participação do sexo masculino entre os agressores (71,8 por cento). Houve correlação positiva entre sexo feminino e abuso sexual e o sexo masculino e abuso físico, inclusive de forma mais severa em relação à presença de lesões corporais. As denúncias foram feitas pelos pais das vítimas (72,9 por cento), sendo o sexo feminino mais freqüente. Neste estudo, não foi identificada nenhuma denúncia realizada pelos profissionais de saúde, refletindo maior necessidade de comprometimento destes na problemática, conforme as recomendações da política de redução da morbimortalidade por acidentes e violência.

This cross-sectional study describes the occurrences of maltreatment recorded over three years (1997-1999) in a Brazilian Police Department for the Protection of the Child and the Adolescent. Socio-demographic and physical data of victims, aggressors and denouncers were investigated in a sample consisting of 2073 cases. The victims were aged between 0-18 years, of which 56.1 percent were girls. Data were entered into EPI INFO 2000 software and analyzed using descriptive statistics and chi-square test to determine significant differences (p<0.05). All manifestations of abuse, except physical abuse, tend to occur more frequently in females, and tend to be more prevalent in the 11-to-15 year-old subgroup. Physical abuse was the most common offense (64.7 percent). Injuries of face and jaw were found in 65.3 percent of the cases. Most of the aggressors were males (71.8 percent). There was a positive correlation between gender (female) and sexual abuse. Physical violence against boys was more intensive, as indicated by the presence of more severe body lesions. Health professionals should increase their diagnostic skills and raise the awareness of their communities to take action for reducing the incidence and the impact of violence upon children and adolescents.

Avaliação dos Conhecimentos e Práticas em Saúde Bucal de Gestantes Atendidas no Setor Público e Privado, em Feira de Santana, Bahia, Brasil / Evaluation of Oral Health Knowledge and Practices of Pregnant Women

Objetivo: Investigar conhecimentos e práticas em saúde bucal em gestantes do município de Feira de Santana/BA. Método: A amostra foi composta por 220 gestantes, das quais 50% realizavam o pré-natal no setor público e 50% no setor privado. Como instrumento de pesquisa foi utilizado um formulário com perguntas abertas e fechadas, a fim de se verificar dados pessoais, características socioeconômicas, conhecimentos e práticas da gestante com relação à própria saúde bucal questionário. A técnica de aplicação foi na forma de entrevista estruturada. A análise estatística foi feita através de distribuição de freqüências, obtenção de médias e desvio padrão, distribuições absolutas e percentuais e o nível de significância 5,0%. Resultados: Um percentual de 51,4% das gestantes tanto na rede pública como na particular, conheciam a doença cárie dentária; gestantes do setor particular apresentaram características socioeconômicas melhoradas, o nível de conhecimento acerca de saúde bucal foi semelhante ao do setor público; relato de sintomas como dor de dente e sangramento gengival, e a associação destes à gravidez, esteve presente nos dois grupos; 81,4% da amostra não recebeu orientações sobre cuidados com a própria saúde bucal e a do filho que vai nascer; sendo evidente a disposição do grupo em adquirir novos conhecimentos, para melhoria das suas próprias condições de saúde bucal e a dos seus filhos. Conclusão: As gestantes apresentaram carência de informações acerca da etiologia dos problemas bucais, métodos de prevenção e possibilidade de tratamento odontológico durante a gravidez. É de extrema importância observar a necessidade de um programa de atenção odontológica que priorize as gestantes, já que o período gestacional torna a mulher mais receptiva a adquirir novos hábitos que refletirão na promoção de saúde bucal de seus filhos.

Efeito da desnutrição protéica pré e pós-natal sobre a morfologia, a diferenciação e o metabolismo do tecido muscular estriado esquelético em ratos / The effects of prenatal and postnatal malnutrition on the morphology, differentiation, and metabolism of skeletal striated muscle tissue in rats

OBJETIVO: Estudar características morfológicas, metabolismo e habilidades contráteis do músculo submetido a desnutrição protéica pré e pós-natal. MÉTODOS: Distribuição dos animais em dois grupos: controle, dieta normoprotéica (GC; n = 15; 5/5/5) e desnutrido, dieta hipoprotéica (GD; n = 15; 5/5/5), observados respectivamente no sétimo, 14º e 28º dia do período experimental. Foram avaliados massa corporal total, peso, habilidades contráteis e a morfologia do músculo tibial anterior. Amostras de tecidos com 8 m de espessura de ratos com idades de 7, 14 e 28 dias, corados por hematoxilina e eosina, e outros submetidos aos métodos histoquímicos nicotinamida adenina tetrazólio redutase (NADH-TR) e miofibrilar (m-ATPase) (pH = 4,4). RESULTADOS: Os pesos corporal e muscular apresentaram-se menores nos grupos desnutridos. Aos 7 dias de desnutrição, o músculo apresentou fibras com menor diâmetro, maior polimorfismo e maior teor de tecido conjuntivo endomisial. Nas histoquímicas, tipos de fibras sem delimitação segura. Aos 14 dias de desnutrição, fibras menores, mais polimórficas, muitas com núcleos centrais e moderado teor de tecido conjuntivo endomisial. Quanto à contração, a reação m-ATPase evidenciou fibras lentas e rápidas. A reação NADH-TR revelou os tipos de fibras slow oxidative, fast oxidative glycolytic e fast glycolytic. Aos 28 dias de desnutrição, fibras menores, agrupadas com contornos variáveis. Quanto ao tipo de contração e metabolismo, os três tipos de fibras apresentaram limites de reconhecimento indistinto. CONCLUSÃO: Os resultados experimentais sugerem que, além da redução no número de fibras, a desnutrição promove um retardamento na diferenciação das características morfológicas, metabólicas e contráteis dos tipos de fibras musculares esqueléticas em ratos na fase de crescimento.

OBJECTIVE: To study the contractile properties, metabolism and morphological characteristics of muscles submitted to prenatal and postnatal protein malnutrition. METHODS: Animals were distributed into two groups: Control, normoprotein diet (CG; n = 15; 5/5/5), and Malnourished, hypoprotein diet (MG; n = 15; 5/5/5), and examined on the 7th, 14th, and 28th days of the experiment. Total body mass, weight, and the contractile properties and morphology of the anterior tibial muscle were assessed. Several 8 µm-thick tissue samples were taken from 7, 14, and 28 day old rats and stained with HE or subjected to NADH-TR or m-ATPase (pH = 4.4) techniques. RESULTS: Body and muscle weight were lower in the malnourished group. On the 7th day of malnutrition, muscle samples exhibited fibers with smaller diameter, higher polymorphism and higher endomysial conjunctive tissue content. Histochemical methods were unable to precisely determine the types of fiber present. On the 14th day, there were smaller muscle fibers, more polymorphism, many of them with central nuclei and moderate endomysial conjunctive tissue content. With reference to contractile properties, the m-ATPase reaction identified both slow and fast fibers. The NADH-TR reaction revealed the following types of fiber: slow oxidative (SO), fast oxidative glycolytic (FOG) and fast glycolytic (FG). On the 28th day smaller, bunched muscle fibers varying shapes. All three types of fiber exhibited unclear recognition limits with respect to contraction and metabolism. CONCLUSION: Our experimental results suggest that, in addition to the reduction in numbers of fibers, malnutrition retards the differentiation of the morphological, metabolic, and contractile characteristics of skeletal muscle fibers in growing rats.

Efeito de diferentes sanificantes sobre a qualidade de morango cv. Oso Grande / Effect of different sanitizing on the quality of strawberry cv. Oso Grande

Conduziu-se o presente trabalho para estudar o efeito de diferentes sanificantes, na conservação pós-colheita de morangos, armazenados sob refrigeração (9± 1ºC e 99 por cento UR), por um período de 12 dias. O delineamento experimental utilizado foi inteiramente casualizado, com 3 repetições, constituindo dos seguintes tratamentos: T1) Controle, imersão em água destilada, 15 min, T2) imersão em solução de dicloro s. triazinatriona sódica dihidratada (Sumaveg®) a 200 ppm, por 15 min; T3) imersão em solução de peróxido de hidrogênio 10 por cento, por 15 min; T4) imersão em solução de dicloro isocianurato de sódio (Hidrosan®) a 200 ppm, por 15 min. As análises de pH, SS, AT, Cor L*a*b e microbiológicas foram realizadas a cada 3 dias. Os processos de sanificação adotados foram eficientes para manter a qualidade de morangos minimamente processados por 12 dias de armazenamento. Verificou-se, no presente trabalho, que o uso de sanificantes foi de fundamental importância para manter baixas as contagens de fungos filamentosos e leveduras dos morangos minimamente processados.

The aim of this work was to study the effect of different sanitizing in the conservation postharvest of strawberry, stored in 9ºC and 99 percent R.H. for 12 days. The experiment was conducted in completely randomized design, with three replicates, the treatments constituted by: T1) Control, immersion in distilled water; T2) immersion in solution of dychlorine s. triazinatriona sodiumdehydrated (Sumaveg®) at 200 ppm, for 15 min; T3) immersion in solution of hydrogen peroxide 10 percent, for 15 min; T4) immersion in solution of dychlorine isocianurate of sodium (Hidrosan®) 200 ppm, for 15 min. The analyses of pH, SS (soluble solids), TA (titrable acidity), Color L*a*b and microbiological was evaluated in 0, 3, 6, 9 and 12 days. The process of sanitizing was efficient to maintain the quality of strawberry minimally processed for 12 days. The use of sanitizing was of fundamental importance to maintain low containing of microorganisms of the group mold and yeasts.