Effect of the green synthesis of CuO plate-like nanoparticles on their photodegradation and antibacterial activities.

Green synthesis of copper oxide nanoparticles and its effects on photocatalytic dye degradation and antibacterial activities are reported. The synthesis of nanoparticles by green routes provides many advantages over chemical routes, including simplicity, cost-effectiveness, and fast processing route without using any costly or harmful chemicals. Tridax procumbense (coat buttons) plant root extract was used to synthesize copper oxide nanoparticles. The synthesized Tridax procumbense-copper oxide nanoparticles (TP-CuO NPs) were characterized by UV-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), dynamic light scattering spectroscopy (DLS), and X-ray diffraction (XRD) techniques. The synthesized TP-CuO NPs were applied for photocatalytic dye degradation and antibacterial activity studies. The TP-CuO NPs exhibited a maximum antibacterial activity at 500 µg mL-1 concentration against Staphylococcus aureus and E. coli showing inhibition zones of 7.5 mm and 7.2 mm, respectively. The photocatalytic ability of the TP-CuO was also tested against the textile dye Trypan blue (TB), and showed about 55% degradation after 48 h for 500 µg mL-1 CuO NP concentration, showing a concentration-dependent degradation efficiency. This is the first work on TP-derived CuO nanoparticles and their photocatalytic and antimicrobial applications. Overall, this study supports the superiority of green-synthesized TP-CuO NPs as photocatalytic and antimicrobial agents.

In vitro anticancer activity of Hirudinaria manillensis methanolic extract and its validation using in silico molecular docking approach.

Cancer has emerged as a potentially lethal illness, which recently upsurged in the mortality rate. Animal-derived compounds could be promising targets with higher efficacy and low toxicity in anticancer therapy. The present study aimed to explore the presence of anticancer potential compounds in Hirudinaria manillensis methanolic extract and their anticancer potential against various cancer cell types and target identification by Auto dock method. Initially, the identification of bioactive compounds was achieved by GC-MS analysis followed by the anticancer activity by MTT assay against A549, HeLa, MDA-MB-231, MG-63, and MOLT-4. Further, the effect of a lead compound on the cancer cell target was analyzed by the Auto dock method. GC-MS analysis results revealed the presence of 25 different bioactive compounds including anticancer potential compounds, such as Lupeol, Carvacrol, and Demecolcine. Interestingly, MTT assay results demonstrated the anticancer potential of Hirudinaria manillensis extract (LE) against various cancer cell lines, such as A549 (54.60 µg/ml), HeLa (19.93 µg/ml), MDA-MB-231 (20.23 µg/ml), MG-63 (20.04 µg/ml), and MOLT-4 (171.8 µg/ml), respectively. Among these cell types, the maximum inhibition was observed against HeLa with the IC50 concentration of 19.93 µg/ml. Furthermore, Demecolcine compound was docked with the EGFR tyrosine kinase showed the binding affinity of the docked complex was predicted to be - 6.2 kcal/mol. Thus, we conclude that H. manillensis has a significant anticancer effect on human cancer cell lines and could be used as a natural target which paves the way for further studies on biomedical applications in cancer therapeutics.