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The current proliferation of mobile robots spans ecological monitoring, warehouse management and extreme environment exploration, to an individual consumer's home1-4. This expanding frontier of applications requires robots to transit multiple environments, a substantial challenge that traditional robot design strategies have not effectively addressed5,6. For example, biomimetic design-copying an animal's morphology, propulsion mechanism and gait-constitutes one approach, but it loses the benefits of engineered materials and mechanisms that can be exploited to surpass animal performance7,8. Other approaches add a unique propulsive mechanism for each environment to the same robot body, which can result in energy-inefficient designs9-11. Overall, predominant robot design strategies favour immutable structures and behaviours, resulting in systems incapable of specializing across environments12,13. Here, to achieve specialized multi-environment locomotion through terrestrial, aquatic and the in-between transition zones, we implemented 'adaptive morphogenesis', a design strategy in which adaptive robot morphology and behaviours are realized through unified structural and actuation systems. Taking inspiration from terrestrial and aquatic turtles, we built a robot that fuses traditional rigid components and soft materials to radically augment the shape of its limbs and shift its gaits for multi-environment locomotion. The interplay of gait, limb shape and the environmental medium revealed vital parameters that govern the robot's cost of transport. The results attest that adaptive morphogenesis is a powerful method to enhance the efficiency of mobile robots encountering unstructured, changing environments.
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Biomimética , Ambiente , Diseño de Equipo , Robótica , Animales , Biomimética/instrumentación , Biomimética/métodos , Locomoción , Robótica/instrumentación , Robótica/métodos , Tortugas/fisiologíaRESUMEN
BACKGROUND: Genomes can be sequenced with relative ease, but ascribing gene function remains a major challenge. Genetically tractable model systems are crucial to meet this challenge. One powerful model is the social amoeba Dictyostelium discoideum, a eukaryotic microbe widely used to study diverse questions in the cell, developmental and evolutionary biology. RESULTS: We describe REMI-seq, an adaptation of Tn-seq, which allows high throughput, en masse, and quantitative identification of the genomic site of insertion of a drug resistance marker after restriction enzyme-mediated integration. We use REMI-seq to develop tools which greatly enhance the efficiency with which the sequence, transcriptome or proteome variation can be linked to phenotype in D. discoideum. These comprise (1) a near genome-wide resource of individual mutants and (2) a defined pool of 'barcoded' mutants to allow large-scale parallel phenotypic analyses. These resources are freely available and easily accessible through the REMI-seq website that also provides comprehensive guidance and pipelines for data analysis. We demonstrate that integrating these resources allows novel regulators of cell migration, phagocytosis and macropinocytosis to be rapidly identified. CONCLUSIONS: We present methods and resources, generated using REMI-seq, for high throughput gene function analysis in a key model system.
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Dictyostelium , Dictyostelium/genética , Genoma , Genómica , TecnologíaRESUMEN
Dictyostelium discoideum (D. discoideum) is a simple eukaryote with a unique life cycle in which it differentiates from unicellular amoebae into a fruiting body upon starvation. Reactive oxygen species (ROS) have been associated with bacterial predation, as well as regulatory events during D. discoideum development and differentiation. Coenzyme A (CoA) is a key metabolic integrator in all living cells. A novel function of CoA in redox regulation, mediated by covalent attachment of CoA to cellular proteins in response to oxidative or metabolic stress, has been recently discovered and termed protein CoAlation. In this study, we report that the level of CoA and protein CoAlation in D. discoideum are developmentally regulated, and correlate with the temporal expression pattern of genes implicated in CoA biosynthesis during morphogenesis. Furthermore, treatment of growing D. discoideum cells with oxidising agents results in a dose-dependent increase of protein CoAlation. However, much higher concentrations were required when compared to mammalian cells and bacteria. Increased resistance of D. discoideum to oxidative stress induced by H2O2 has previously been attributed to high levels of catalase activity. In support of this notion, we found that H2O2-induced protein CoAlation is significantly increased in CatA-deficient D. discoideum cells. Collectively, this study provides insights into the role of CoA and protein CoAlation in the maintenance of redox homeostasis in amoeba and during D. discoideum morphogenesis.
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Coenzima A/metabolismo , Dictyostelium/crecimiento & desarrollo , Estrés Oxidativo , Proteínas Protozoarias/metabolismo , Dictyostelium/citología , Dictyostelium/metabolismo , Humanos , Peróxido de Hidrógeno/metabolismo , Morfogénesis , Oxidación-Reducción , Procesamiento Proteico-Postraduccional , Infecciones por Protozoos/parasitología , Especies Reactivas de Oxígeno/metabolismoRESUMEN
Numerous recent research efforts have leveraged networks of rigid struts and flexible cables, called tensegrity structures, to create highly resilient and packable mobile robots. However, the locomotion of existing tensegrity robots is limited in terms of both speed and number of distinct locomotion modes, restricting the environments that a robot is capable of exploring. In this study, we present a tensegrity robot inspired by the volumetric expansion of Tetraodontidae. The robot, referred to herein as Spikebot, employs pneumatically actuated rigid struts to expand its global structure and produce diverse gaits. Spikebot is composed of linear actuators that dually serve as rigid struts linked by elastic cables for stability. The linearly actuating struts can selectively protrude to initiate thrust- and instability-driven locomotion primitives. Such motion primitives allow Spikebot to reliably locomote, achieving rolling, lifting, and jumping. To highlight Spikebot's potential for robotic exploration, we demonstrate how it achieves multi-dimensional locomotion over varied terrestrial conditions.
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Allelochemicals represent a class of natural products released by plants as root, leaf, and fruit exudates that interfere with the growth and survival of neighboring plants. Understanding how allelochemicals function to regulate plant responses may provide valuable new approaches to better control plant function. One such allelochemical, Myrigalone A (MyA) produced by Myrica gale, inhibits seed germination and seedling growth through an unknown mechanism. Here, we investigate MyA using the tractable model Dictyostelium discoideum and reveal that its activity depends on the conserved homolog of the plant ethylene synthesis protein 1-aminocyclopropane-1-carboxylic acid oxidase (ACO). Furthermore, in silico modeling predicts the direct binding of MyA to ACO within the catalytic pocket. In D. discoideum, ablation of ACO mimics the MyA-dependent developmental delay, which is partially restored by exogenous ethylene, and MyA reduces ethylene production. In Arabidopsis thaliana, MyA treatment delays seed germination, and this effect is rescued by exogenous ethylene. It also mimics the effect of established ACO inhibitors on root and hypocotyl extension, blocks ethylene-dependent root hair production, and reduces ethylene production. Finally, in silico binding analyses identify a range of highly potent ethylene inhibitors that block ethylene-dependent response and reduce ethylene production in Arabidopsis. Thus, we demonstrate a molecular mechanism by which the allelochemical MyA reduces ethylene biosynthesis and identify a range of ultrapotent inhibitors of ethylene-regulated responses.
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Arabidopsis , Etilenos , Feromonas , Etilenos/biosíntesis , Etilenos/metabolismo , Feromonas/farmacología , Feromonas/metabolismo , Arabidopsis/genética , Arabidopsis/metabolismo , Arabidopsis/efectos de los fármacos , Germinación/efectos de los fármacosRESUMEN
Programming inflatable systems to deform to desired 3D shapes opens up multifarious applications in robotics, morphing architecture, and interventional medicine. This work elicits complex deformations by attaching discrete strain limiters to cylindrical hyperelastic inflatables. Using this system, a method is presented to solve the inverse problem of programming myriad 3D centerline curves upon inflation. The method entails two steps: first, a reduced-order model generates a conceptual solution giving coarse indications of strain limiter placement on the undeformed cylindrical inflatable. This low-fidelity solution then seeds a finite element simulation nested within an optimization loop to further tune strain limiter parameters. We leverage this framework to achieve functionality through a priori programmed deformations of cylindrical inflatables, including 3D curve matching, self-tying knotting, and manipulation. The results hold broad significance for the emerging computational design of inflatable systems.
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Owing to the remarkable properties of the somatosensory system, human skin compactly perceives myriad forms of physical stimuli with high precision. Machines, conversely, are often equipped with sensory suites constituted of dozens of unique sensors, each made for detecting limited stimuli. Emerging high degree-of-freedom human-robot interfaces and soft robot applications are delimited by the lack of simple, cohesive, and information-dense sensing technologies. Stepping toward biological levels of proprioception, we present a sensing technology capable of decoding omnidirectional bending, compression, stretch, binary changes in temperature, and combinations thereof. This multi-modal deformation and temperature sensor harnesses chromaticity and intensity of light as it travels through patterned elastomer doped with functional dyes. Deformations and temperature shifts augment the light chromaticity and intensity, resulting in a one-to-one mapping between stimulus modes that are sequentially combined and the sensor output. We study the working principle of the sensor via a comprehensive opto-thermo-mechanical assay, and find that the information density provided by a single sensing element permits deciphering rich and diverse human-robot and robot-environmental interactions.
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Piel , Dispositivos Electrónicos Vestibles , Humanos , Temperatura , Propiocepción , Sensación TérmicaRESUMEN
Numerous recent advances in robotics have been inspired by the biological principle of tensile integrity-or "tensegrity"-to achieve remarkable feats of dexterity and resilience. Tensegrity robots contain compliant networks of rigid struts and soft cables, allowing them to change their shape by adjusting their internal tension. Local rigidity along the struts provides support to carry electronics and scientific payloads, while global compliance enabled by the flexible interconnections of struts and cables allows a tensegrity to distribute impacts and prevent damage. Numerous techniques have been proposed for designing and simulating tensegrity robots, giving rise to a wide range of locomotion modes, including rolling, vibrating, hopping, and crawling. In this study, we review progress in the burgeoning field of tensegrity robotics, highlighting several emerging challenges, including automated design, state sensing, and kinodynamic motion planning.
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Robótica , Electrónica , Locomoción , Movimiento (Física) , Robótica/métodosRESUMEN
A critical aspect of toxicity evaluation is developmental and reproductive toxicity (DART) testing. Traditionally, DART testing has been conducted in vivo in mammalian model systems. New legislation aimed at reducing animal use and the prohibitive costs associated with DART testing, together with a need to understand the genetic pathways underlying developmental toxicity means there is a growing demand for alternative model systems for toxicity evaluation. Here we explore the potential of the eukaryotic social amoeba Dictyostelium discoideum, which is already widely used as a simple model system for cell and developmental biology, as a potential nonanimal model for DART testing. We developed assays for high-throughput screening of toxicity during D. discoideum growth and development. This allowed the toxicity of a broad range of test compounds to be characterized, which revealed that D. discoideum can broadly predict mammalian toxicity. In addition, we show that this system can be used to perform functional genomic screens to compare the molecular modes of action of different compounds. For example, genome-wide screens for mutations that affect lithium and valproic acid toxicity allowed common and unique biological targets and molecular processes mediating their toxicity to be identified. These studies illustrate that D. discoideum could represent a predictive nonanimal model for DART testing due to its amenability to high-throughput approaches and molecular genetic tractability.
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Dictyostelium , Animales , Dictyostelium/genética , Mutación , Reproducción , Pruebas de Toxicidad , Ácido Valproico/toxicidadRESUMEN
The emerging generation of robots composed of soft materials strives to match biological motor adaptation skills via shape-shifting. Soft robots often harness volumetric expansion directed by strain limiters to deform in complex ways. Traditionally, strain limiters have been inert materials embedded within a system to prescribe a single deformation. Under changing task demands, a fixed deformation mode limits adaptability. Recent technologies for on-demand reprogrammable deformation of soft bodies, including thermally activated variable stiffness materials and jamming systems, presently suffer from long actuation times or introduce unwanted bending stiffness. We present fibers that switch tensile stiffness via jamming of segmented elastic fibrils. When jammed, tensile stiffness increases more than 20× in less than 0.1 s, but bending stiffness increases only 2×. When adhered to an inflating body, jamming fibers locally limit surface tensile strains, unlocking myriad programmable deformations. The proposed jamming technology is scalable, enabling adaptive behaviors in emerging robotic materials that interact with unstructured environments.
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Robotic vehicles capable of transition from aquatic to terrestrial locomotion face considerable challenges associated with propulsive efficiency and performance in each environment. Here we present a morphing amphibious robotic limb that combines the locomotor adaptations of sea turtles for swimming and tortoises for walking. The limb can transform between the streamlined morphology of a sea turtle flipper and the load-bearing geometry of a tortoise leg using a variable stiffness material coupled to a pneumatic actuator system. Herein, we describe the fabrication and characterization of the morphing limb, and quantitatively show how morphing between hydrodynamic and axial-load bearing states can enhance the locomotive performance of a single design over land and in water.
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Extremidades/fisiología , Robótica/instrumentación , Tortugas/fisiología , Animales , Fenómenos Biomecánicos , Diseño de Equipo , Hidrodinámica , Modelos Biológicos , Natación , CaminataRESUMEN
Compliant, continuum structures allow living creatures to perform complex tasks inaccessible to artificial rigid systems. Although advancements in hyper-elastic materials have spurred the development of synthetic soft structures (i.e., artificial muscles), these structures have yet to match the precise control and diversity of motions witnessed in living creatures. Cephalopods tentacles, for example, can undergo multiple trajectories using muscular hydrostat, a structure consisting of aggregated laminae of unidirectional muscle fibers. Here, we present a self-adhesive composite lamina inspired by the structural morphology of the muscular hydrostat, which adheres to any volumetrically expanding soft body to govern its motion trajectory. The composite lamina is stretchable only in one direction due to inextensible continuous fibers unidirectionally embedded within its hyper-elastic matrix. We showcase reconfiguration of inflation trajectories of two- and three-dimensional soft bodies by simply adhering laminae to their surfaces.
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Cefalópodos/química , Cefalópodos/fisiología , Fibras Musculares Esqueléticas/fisiología , Animales , Órganos Artificiales , Fenómenos Biomecánicos , Materiales Biomiméticos/química , Polímeros , Ingeniería de TejidosRESUMEN
Dual-functional electrocatalysts have recently been reported to improve the conversion and storage of energy generated from overall water splitting in alkaline electrolytes. Herein, for the first time, a shape-controlled synthesis of starfish-like Co-Ni-S nanosheets on three-dimensional (3D) hierarchically porous nickel foams (Co-Ni-S/NF) via a one-step hydrothermal method was developed. The influence of reaction time on the nanosheet structure and properties was intensively studied. After 11 h reaction, the Co-Ni-S/NF-11 sample displays the most regular structure of nanosheets and the most outstanding electrochemical properties. As to water splitting, hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) required overpotentials of 284.3 and 296 mV, respectively, to provide a current density of 100 mA cm-2. The marvelous electrochemical performance can be attributed to the conductive networks of 3D layered porous nickel skeletons that are highly interconnected, which provided a large specific area and highly active sites. To further enhance the electrochemical performances of the electrocatalyst, the influence of the doping of the P element was also studied. The results proved that the P-doped Co-Ni-S/NF maintains the starfish structure and demonstrates outstanding properties, providing a current density of 100 mA cm-2 with only 187.4 and 292.2 mV overpotentials for HER and OER, respectively. It exhibited far more excellent properties than reported dual-functional electrocatalysts. Additionally, when used as an overall water-splitting catalyst, P-Co-Ni-S/NF can provide a 10 mA cm-2 current density at a given cell voltage of 1.60 V in 1 M KOH, which is competitive to the best-known electrocatalysts, with high long-term stability.
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High-performance active materials for energy-storage and energy-conversion applications require a novel class of electrodes: ones with a structure conducive to conductivity, large specific surface area, high porosity, and mechanical robustness. Herein, we report the design and fabrication of a new ternary hybrid aerogel. The process entails an in situ assembly of 2D WSe2 nanosheets and NiFe-LDH nanosheets on a 3D N,S-codoped graphene framework, accomplished by a facile hydrothermal method and electrostatic self-assembly technology. The obtained nanocomposite architecture maximizes synergistic effects among its three 2D-layer components. To assess the performance of this hybrid material, we deployed it as an advanced electrode in overall water splitting and in a supercapacitor. Results in both scenarios attest to its excellent electrochemical properties. Specifically, serving as a catalyst in an oxygen evolution reaction, our nanocomposite requires overpotentials of 1.48 and 1.59 V to obtain current densities of 10 and 100 mA cm-2, respectively. The hybrid material also efficiently electrocatalyzes hydrogen evolution reactions in base solution, necessitating overpotentials of -50 and -237 mV for current densities of 1.0 and 100 mA cm-2, respectively. The 3D hybrid, when applied to a symmetric supercapacitor device, achieves 125.6 F g-1 capacitance at 1 A g-1 current density. In summary, our study elucidates a new strategy to maximize efficiency via synergetic effects that is likely applicable to other 2D materials.
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Direct liquid phase exfoliation (LPE) is generally regarded as an effective and efficient methodology for preparing single- to few-layered nanosheets on a large scale. Based on a previous finding that the polar and dispersive components of surface tension can be used as critical parameters for screening suitable solvents for LPE, in this study, we conducted in-depth research on direct LPE of two-dimensional (2D) materials by the extensive LPE of a series of 2D materials and the thorough comparison of their surfaces properties and LPE efficiencies. We rationally developed the surface tension component matching (STCM) theory, and in nature, its key point lies in the close ratio of polar to dispersive components (P/D) between the solvents and the aimed 2D materials. To this end, the surface tension components ratio is demonstrated to be an effective parameter for screening LPE solvents. In addition to the optimization of the LPE process for these 2D materials, this work has further greatly enlarged the comprehensive library for the solvent and 2D material matching pairs based on the improved STCM theory.
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Developing efficient visible-light-driven photocatalysts will advance alternative energy technologies, ultimately curbing the environmental pollution associated with fossil fuels. In this work, Bi2S3/Bi2MoO6 photocatalysts with a heterogeneous cookie-like structure were prepared for the first time by in-situ anion exchange at relatively low temperatures. The catalysts exhibited enhanced photocatalytic activity, which we attributed to the photocurrent response, a diminished recombination rate of photogenerated electron-hole pairs, and the existence of a large heterojunction interface. These governing factors were discerned by photoelectrochemical measurements, calculated energy band positions and photoluminescence spectra. Bi2S3/Bi2MoO6 nanocomposites also exhibit better performance in response to gas than bare Bi2MoO6 according to gas sensing tests. Our work, in relaying a feasible method to synthesize Bi2S3/Bi2MoO6-based heterojunction superstructures, and documents a universal preparation method of synthetic heterogeneous complexes, and provides necessary groundwork for the development of next generation semiconductor photocatalytic technology and gas sensor.
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Transition metal dichalcogenides, MX2 (M = Fe, Co, Ni, X = S, Se, Te), have been proven to be promising substitutes for noble metals in hydrogen evolution reactions (HERs). However, forthright comparisons of metal sulfides, metal selenides, and metal tellurides are rarely conducted, let alone the mechanism of the important role of their non-metal ligands. In this paper, we report the pilot study of a controllable method for the preparation of a series of NiX2 (X = S, Se, Te) nanosheets via a facile anion-exchange reaction. Consequently, the HER activities and stabilities of NiS2, NiSe2, and NiTe2 nanosheets were tested in both acid and alkaline solutions. The required overpotentials to reach 10 mA cm-2 in 0.5 M H2SO4 for NiS2, NiSe2, and NiTe2 were 213, 156, and 276 mV, respectively. The best performance of NiSe2 was also confirmed in 1 M KOH. Besides NiS2 and NiTe2 nanosheets, the HER properties of NiSe2 nanosheets are superior to most of the available nickel catalysts. Interestingly, the results from electrochemical measurements were found to be fully consistent with the data based on density function theory calculation. Among various factors that might influence the HER activities of nickel dichalcogenides, the free energies of hydrogen adsorption and conductivities have played important roles.
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Synthesis of highly efficient and robust catalysts with earth-abundant resources for overall water splitting is essential for large-scale energy conversion processes. Herein, a series of highly active and inexpensive Co-Ni-P films were fabricated by a one-step constant current density electrodeposition method. These films were demonstrated to be efficient bifunctional catalysts for both H2 and O2 evolution reactions (HER and OER), while deposition time was deemed to be the crucial factor governing electrochemical performance. At the optimal deposition time, the obtained Co-Ni-P-2 catalyst performed remarkably for both HER and OER in alkaline media. In particular, it requires -103 mV overpotential for HER and 340 mV for OER to achieve the current density of 10 mA cm-2, with corresponding Tafel slopes of 33 and 67 mV dec-1. Moreover, it outperforms the Pt/C//RuO2 catalyst and only needs -160 mV (430 mV) overpotential for HER (OER) to achieve 200 mA cm-2 current density. Co-Ni-P electrodes were also conducted for the proof-of-concept exercise, which were proved to be flexible, stable, and efficient, further opening a new avenue for rapid synthesis of efficient, flexible catalysts for renewable energy resources.
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Novel ternary electrode materials based on graphene, NiCo2S4, and transition metal dichalcogenides (TMDs) were designed and fabricated with the intention of exploiting synergistic effects conducive to supercapacitive energy storage. Compared to NiCo2S4-g-MoSe2, the NiCo2S4-g-MoS2 electrode exhibited higher specific capacitance, enhanced rate capability (1002 F g(-1) even at 5 A g(-1), 6.01 F cm(-2) at a current density of 25 mA cm(-2)) and cycling stability (94.8% retention of its original capacity after cycling 4000 times). The mechanism was proposed and this pioneering work will be helpful in making judicious choices of which 2D materials to be selected for supercapacitor applications in the future.