Cryoplasty versus angioplasty in the treatment of arterial restenosis in an experimental model of atherosclerosis in rabbits.

Cryoplasty may reduce the incidence of post-angioplasty restenosis in peripheral atherosclerotic arteries. Our study is looking to investigate the mid-term effects (4 weeks) of an FDA-approved cryoplasty catheter (PolarCath(®), Boston Scientific) compared to a conventional angioplasty catheter using a hypercholesterolemic rabbit model of arterial restenosis based on diet plus vessel injury. Thirty-six normolipidemic, 3-month old male New Zealand White rabbits were used. Balloon angioplasty was performed on left external iliac arteries on day 1. Animals were fed with a hypercholesterolemic diet for 60 days. On day 120, three groups of animals were established: conventional PTA (percutaneous transluminal angioplasty) was applied on the PTA group; the CRY group was treated with the PolarCath(®) cryoplasty system and no treatment was given to a control (CTR) group. A broad variety of atheromatous lesions were observed 30 days after treatment, presenting significant differences between groups. Most of the complicated lesions were found in the CRY group, while advanced and early lesions were more often appreciated in the CTR and PTA groups, respectively. The histomorphometric evaluation of the arteries showed significant differences between the CRY group and the other two groups, with the highest percentage of IEM (internal elastic membrane) injury, vascular stenosis and ratio intima/media being registered on animals treated with cryoplasty. Intravascular cryotherapy induces complicated lesions in arterial walls 30 days after treatment in a hypercholesterolemic rabbit model based on diet plus vessel injury. Cryoplasty leads to the production of severe fibrosis and mineralisation and stenosis compared to a conventional angioplasty.

Anatomical variations in the aortic bifurcation in new zealand white rabbits on arteriography.

The radiologic anatomy of the aortic bifurcation in the rabbit has received little study but it is important as this anatomical area is widely used in atherosclerosis research. Thirty rabbits were used to study the aortic bifurcation and subsequent branching patterns on arteriography. Fifteen different arteries were identified. Mean arterial diameters of 2.88 ± 0.7 and 2.27 ± 0.55 mm were obtained for the aorta and external iliac arteries, respectively. The cranial and middle aspects at the seventh lumbar vertebra (L7) were the most frequent anatomical landmarks (53.3% of the cases) for aortic and common iliac bifurcations, respectively. The caudal aspect of L6 was the most frequent origin (50% of the cases) for the median sacral artery. Deep circumflex iliac arteries originated from common iliac arteries and not the abdominal aorta in the rabbit, showing anatomical asymmetry in 73.3% of the cases. No gender disparity was found in the anatomical location of any of the arteries of the study. Knowledge of normal vascular landmarks for the aortic bifurcation as well as anatomical variations should be helpful to future experimental studies.