Stability of cefepime in aqueous eye drops.

The aim of the studies was to determine HPLC the stability of cefepime in 1% and 5% buffered eye drops of developed formulary composition, which were stored for 30 days at the temperature of 4 degrees C and 20 degrees C, protected from light. Separation was performed on RP18 Gemini octadecylsilane column (250 mm x 4.6 mm, 5.0 microm) at a temperature of 25 degrees C. The mobile phase consisted of 0.015 M solution of sodium salt of pentane sulphonic acid brought to pH 4.0 with glacial acetic acid and 45% KOH solution and acetonitrile 94:6 w/w, with detection of 254 nm. The method was linear in the range of 12.6-125 microg/ml (R2 = 0.9996). The limit of detection (LOD) was 3 microg/ml and limit of quantification (LOQ) was 10 microg/ml. 10% degradation of cefepime in 1% and 5% buffered eye drops stored at the temperature of 4 degrees C, depending on the composition of the eye drops, occurred after 21-27 days in 1% eye drops and 18-21 days in 5% eye drops. In the eye drops, which were stored at the temperature of 20 degrees C, 10% degradation of cefepime took place on the third day of storage regardless of formulary composition of 1% and 5% drops. Cefepime stability lasting a couple of weeks in 1% and 5% solution allows extemporaneous preparation of buffered eye drops containing cefepime.

The interrelationship between production of reactive oxygen intermediates and inflammatory cytokines by peripheral blood leukocytes in vitro from children with food allergy.

In this study, the activity of neutrophils measured using the chemiluminescence test was estimated in 49 children with food allergy. It was shown that average chemiluminescence values without, as well as after, stimulation by formyl-methionyl-leucine phenylalanine and phorbol myristate acetate were considerably higher in children with allergy in comparison to those in the control group. The correlation between the production of reactive oxygen intermediates and interleukin (IL)-4, IL-5 and IL-10 concentration suggests that an autocrine regulatory mechanism may play a role in allergy in children.

[Evaluation of peripheral blood neutrophils activity in women with endometriosis]. / Ocena aktywnosci neutrofilów krwi obwodowej u kobiet z endometrioza.

Authors evaluated the neutrophils ability of creating reactive oxygen intermediates (ROI) in peripheral blood of 14 patients with endometriosis. These tests were performed using the chemiluminescence method. Enhanced ROI production and in vivo priming phenomenon of neutrophils in endometriosis were reported. The observed increase in oxygen activity of neutrophils was statistically significant only in reaction with the use of fMLP. However performed neutrophils preactivation with TNF-alpha followed by the chemiluminescence test revealed the occurrence of in vivo neutrophil activation in women with endometriosis. Reported data confirm the existence of local inflammatory reaction in the course of endometriosis.

[Evaluation of the generation of interleukin-10 by peripheral blood lymphocytes in women with endometriosis]. / Ocena generacji interleukiny-10 przez limfocyty krwi obwodowej u kobiet z endometrioza.

The aim of study was the evaluation of generating the IL-10 by peripheral blood lymphocytes in women with endometriosis according to the stage of of the disease. Cytokine was estimated by immunoenzymatic method Elisa. The generation of IL-10 by peripheral blood lymphocytes was lower in patients with all stages of endometriosis than in control group. Decreased IL-10 generating observed in our study was not statistically significant, but it may correlate with disorders of immunological cell response. It may also play a role in a development and progression of endometriosis.