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1.
Cell ; 158(2): 353-367, 2014 Jul 17.
Artículo en Inglés | MEDLINE | ID: mdl-25036632

RESUMEN

Hermes is a member of the hAT transposon superfamily that has active representatives, including McClintock's archetypal Ac mobile genetic element, in many eukaryotic species. The crystal structure of the Hermes transposase-DNA complex reveals that Hermes forms an octameric ring organized as a tetramer of dimers. Although isolated dimers are active in vitro for all the chemical steps of transposition, only octamers are active in vivo. The octamer can provide not only multiple specific DNA-binding domains to recognize repeated subterminal sequences within the transposon ends, which are important for activity, but also multiple nonspecific DNA binding surfaces for target capture. The unusual assembly explains the basis of bipartite DNA recognition at hAT transposon ends, provides a rationale for transposon end asymmetry, and suggests how the avidity provided by multiple sites of interaction could allow a transposase to locate its transposon ends amidst a sea of chromosomal DNA.


Asunto(s)
Elementos Transponibles de ADN , Moscas Domésticas/enzimología , Transposasas/química , Animales , Secuencia de Bases , Cristalografía por Rayos X , Dimerización , Moscas Domésticas/genética , Proteínas de Insectos/química , Proteínas de Insectos/genética , Proteínas de Insectos/metabolismo , Modelos Moleculares , Datos de Secuencia Molecular , Proteínas de Unión al ARN/química , Proteínas de Unión al ARN/genética , Proteínas de Unión al ARN/metabolismo , Transposasas/genética , Transposasas/metabolismo
2.
Chem Rev ; 122(10): 9307-9330, 2022 05 25.
Artículo en Inglés | MEDLINE | ID: mdl-34766756

RESUMEN

The measurement and application of residual dipolar couplings (RDCs) in solution NMR studies of biological macromolecules has become well established over the past quarter of a century. Numerous methods for generating the requisite anisotropic orientational molecular distribution have been demonstrated, each with its specific strengths and weaknesses. In parallel, an enormous number of pulse schemes have been introduced to measure the many different types of RDCs, ranging from the most widely measured backbone amide 15N-1H RDCs, to 1H-1H RDCs and couplings between low-γ nuclei. Applications of RDCs range from structure validation and refinement to the determination of relative domain orientations, the measurement of backbone and domain motions, and de novo structure determination. Nevertheless, it appears that the power of the RDC methodology remains underutilized. This review aims to highlight the practical aspects of sample preparation and RDC measurement while describing some of the most straightforward applications that take advantage of the exceptionally precise information contained in such data. Some emphasis will be placed on more recent developments that enable the accurate measurement of RDCs in larger systems, which is key to the ongoing shift in focus of biological NMR spectroscopy from structure determination toward gaining improved understanding of how molecular flexibility drives protein function.


Asunto(s)
Proteínas , Espectroscopía de Resonancia Magnética/métodos , Resonancia Magnética Nuclear Biomolecular/métodos , Proteínas/química
3.
J Am Chem Soc ; 2023 Feb 08.
Artículo en Inglés | MEDLINE | ID: mdl-36753641

RESUMEN

Post-translational modifications are ubiquitous in the eukaryotic proteome. However, these modifications are rarely incorporated in NMR studies of eukaryotic proteins, which are typically produced through recombinant expression in E. coli. Melittin is the primary peptide in honey bee venom. Its native C-terminal amide significantly affects its equilibrium structure and dynamics in solution and is thus a prerequisite for studying its native structure and function. Here, we present a method for producing triply isotopically labeled (2H, 13C, and 15N) native melittin through recombinant expression followed by chemical amidation. We then show that structural models produced with AlphaFold-Multimer are in even better agreement with experimental residual dipolar couplings than the 2.0 Å resolution X-ray crystal structure for residues G3-K23.

4.
J Am Chem Soc ; 145(32): 18063-18074, 2023 08 16.
Artículo en Inglés | MEDLINE | ID: mdl-37548612

RESUMEN

Amelotin is an intrinsically disordered protein (IDP) rich in Pro residues and is involved in hydroxyapatite mineralization. It rapidly oligomerizes under physiological conditions of pH and pressure but reverts to its monomeric IDP state at elevated pressure. We identified a 105-residue segment of the protein that becomes ordered upon oligomerization, and we used pressure-jump NMR spectroscopy to measure long-range NOE contacts that exist exclusively in the oligomeric NMR-invisible state. The kinetics of oligomerization and dissociation were probed at the residue-specific level, revealing that the oligomerization process is initiated in the C-terminal half of the segment. Using pressure-jump NMR, the degree of order in the oligomer at the sites of Pro residues was probed by monitoring changes in cis/trans equilibria relative to the IDP state after long-term equilibration under oligomerizing conditions. Whereas most Pro residues revert to trans in the oligomeric state, Pro-49 favors a cis configuration and three Pro residues retain an unchanged cis fraction, pointing to their local lack of order in the oligomeric state. NOE contacts and secondary 13C chemical shifts in the oligomeric state indicate the presence of an 11-residue α-helix, preceded by a small intramolecular antiparallel ß-sheet, with slower formation of long-range intermolecular interactions to N-terminal residues. Although none of the models generated by AlphaFold2 for the amelotin monomer was consistent with experimental data, subunits of a hexamer generated by AlphaFold-Multimer satisfied intramolecular NOE and chemical shift data and may provide a starting point for developing atomic models for the oligomeric state.


Asunto(s)
Prolina , Proteínas , Conformación Proteica , Isomerismo , Prolina/química , Espectroscopía de Resonancia Magnética/métodos
5.
Anal Chem ; 94(15): 5729-5733, 2022 04 19.
Artículo en Inglés | MEDLINE | ID: mdl-35394743

RESUMEN

Due to similar reactivity of organic hydroperoxides (OHPs), an HPLC separation step is typically required for their indirect (chemical) quantification in mixtures. The high sensitivity of chemical shifts to chemical structure makes NMR an ideal tool for the simultaneous quantification of OHPs in mixtures, but the concentration of these analytes in the samples of interest is usually well below the sensitivity of standard NMR experiments. This sensitivity problem can be mitigated by taking advantage of the fact that the z magnetization of the H2O2 resonance recovers at the rate of hydrogen exchange with water, which is significantly faster than longitudinal relaxation, thus enabling very fast scanning for signal-to-noise enhancement. An adaptation of the E-BURP2 pulse is described that suppresses the water signal by more than 4 orders of magnitude, yielding uniform excitation of peroxide signals without interference of the ca. 108-fold stronger H2O resonance. We demonstrate the method for a mixture of OHPs and report the chemical shifts for multiple OHPs that are of interest in atmospheric chemistry. As shown for hydroxymethyl hydroperoxide, the chemical decay of OHPs can be tracked directly by NMR spectroscopy.


Asunto(s)
Peróxido de Hidrógeno , Imagen por Resonancia Magnética , Hidrógeno , Peróxido de Hidrógeno/química , Espectroscopía de Resonancia Magnética/métodos , Agua
6.
Chembiochem ; 23(19): e202200471, 2022 10 06.
Artículo en Inglés | MEDLINE | ID: mdl-35972230

RESUMEN

The 68-kDa homodimeric 3C-like protease of SARS-CoV-2, Mpro (3CLpro /Nsp5), is a key antiviral drug target. NMR spectroscopy of this large system proved challenging and resonance assignments have remained incomplete. Here we present the near-complete (>97 %) backbone assignments of a C145A variant of Mpro (Mpro C145A ) both with, and without, the N-terminal auto-cleavage substrate sequence, in its native homodimeric state. We also present SILLY (Selective Inversion of thioL and Ligand for NOESY), a simple yet effective pseudo-3D NMR experiment that utilizes NOEs to identify interactions between Cys-thiol or aliphatic protons, and their spatially proximate backbone amides in a perdeuterated protein background. High protection against hydrogen exchange is observed for 10 of the 11 thiol groups in Mpro C145A , even those that are partially accessible to solvent. A combination of SILLY methods and high-resolution triple-resonance NMR experiments reveals site-specific interactions between Mpro , its substrate peptides, and other ligands, which present opportunities for competitive binding studies in future drug design efforts.


Asunto(s)
COVID-19 , Protones , Amidas , Antivirales/química , Proteasas 3C de Coronavirus , Cisteína Endopeptidasas/metabolismo , Humanos , Ligandos , Espectroscopía de Resonancia Magnética , Péptidos/metabolismo , Inhibidores de Proteasas , SARS-CoV-2 , Solventes , Compuestos de Sulfhidrilo
7.
Biophys J ; 120(6): 994-1000, 2021 03 16.
Artículo en Inglés | MEDLINE | ID: mdl-33582134

RESUMEN

The seasonality of respiratory diseases has been linked, among other factors, to low outdoor absolute humidity and low indoor relative humidity, which increase evaporation of water in the mucosal lining of the respiratory tract. We demonstrate that normal breathing results in an absorption-desorption cycle inside facemasks, in which supersaturated air is absorbed by the mask fibers during expiration, followed by evaporation during inspiration of dry environmental air. For double-layered cotton masks, which have considerable heat capacity, the temperature of inspired air rises above room temperature, and the effective increase in relative humidity can exceed 100%. We propose that the recently reported, disease-attenuating effect of generic facemasks is dominated by the strong humidity increase of inspired air. This elevated humidity promotes mucociliary clearance of pathogens from the lungs, both before and after an infection of the upper respiratory tract has occurred. Effective mucociliary clearance can delay and reduce infection of the lower respiratory tract, thus mitigating disease severity. This mode of action suggests that masks can benefit the wearer even after an infection in the upper respiratory tract has occurred, complementing the traditional function of masks to limit person-to-person disease transmission. This potential therapeutical use should be studied further.


Asunto(s)
COVID-19/patología , COVID-19/prevención & control , Máscaras/virología , Sistema Respiratorio/virología , Índice de Severidad de la Enfermedad , COVID-19/virología , Humanos , Humedad , SARS-CoV-2/fisiología
8.
J Am Chem Soc ; 143(46): 19306-19310, 2021 11 24.
Artículo en Inglés | MEDLINE | ID: mdl-34757725

RESUMEN

The 68-kDa homodimeric 3C-like protease of SARS-CoV-2, Mpro (3CLpro/Nsp5), is a promising antiviral drug target. We evaluate the concordance of models generated by the newly introduced AlphaFold2 structure prediction program with residual dipolar couplings (RDCs) measured in solution for 15N-1HN and 13C'-1HN atom pairs. The latter were measured using a new, highly precise TROSY-AntiTROSY Encoded RDC (TATER) experiment. Three sets of AlphaFold2 models were evaluated: (1) MproAF, generated using the standard AlphaFold2 input structural database; (2) MproAFD, where the AlphaFold2 implementation was modified to exclude all candidate template X-ray structures deposited after Jan 1, 2020; and (3) MproAFS, which excluded all structures homologous to coronaviral Mpro. Close agreement between all three sets of AlphaFold models and experimental RDC data is found for most of the protein. For residues in well-defined secondary structure, the agreement decreases somewhat upon Amber relaxation. For these regions, MproAF agreement exceeds that of most high-resolution X-ray structures. Residues from domain 2 that comprise elements of both the active site and the homo-dimerization interface fit less well across all structures. These results indicate novel opportunities for combining experimentation with molecular dynamics simulations, where solution RDCs provide highly precise input for QM/MM simulations of substrate binding/reaction trajectories.


Asunto(s)
Proteasas 3C de Coronavirus/química , Cristalografía por Rayos X/métodos , SARS-CoV-2 , COVID-19 , Dominio Catalítico , Espectroscopía de Resonancia Magnética , Conformación Molecular , Simulación de Dinámica Molecular , Conformación Proteica , Pliegue de Proteína , Programas Informáticos , Rayos X
9.
Angew Chem Int Ed Engl ; 60(1): 166-170, 2021 01 04.
Artículo en Inglés | MEDLINE | ID: mdl-32916024

RESUMEN

The membrane proximal external region (MPER) of HIV-1 gp41 contains epitopes for at least four broadly neutralizing antibodies. Depending on solution conditions and construct design, different structures have been reported for this segment. We show that in aqueous solution the MPER fragment (gp160660-674 ) exists in a monomer-trimer equilibrium with an association constant in the micromolar range. Thermodynamic analysis reveals that the association is exothermic, more favorable in D2 O than H2 O, and increases with ionic strength, indicating hydrophobically driven intermolecular interactions. Circular dichroism, 13 Cα chemical shifts, NOE, and hydrogen exchange rates reveal that MPER undergoes a structural transition from predominately unfolded monomer at low concentrations to an α-helical trimer at high concentrations. This result has implications for antibody recognition of MPER prior to and during the process where gp41 switches from a pre-hairpin intermediate to its post-fusion 6-helical bundle state.


Asunto(s)
Proteína gp41 de Envoltorio del VIH/química , VIH-1/química , Péptidos/química , Conformación Proteica en Hélice alfa/fisiología , Humanos
10.
Biophys J ; 118(5): 1119-1128, 2020 03 10.
Artículo en Inglés | MEDLINE | ID: mdl-32049057

RESUMEN

Highly charged, single α-helical (SAH) domains contain a high percentage of Arg, Lys, and Glu residues. Their dynamic salt bridge pairing creates the exceptional stiffness of these helical rods, with a persistence length of more than 200 Å for the myosin VI SAH domain. With the aim of modulating the stiffness of the helical structure, we investigated the effect, using NMR spectroscopy, of substituting key charged Arg, Lys, Glu, and Asp residues by Gly or His. Results indicate that such mutations result in the transient breaking of the helix at the site of mutation but with noticeable impact on amide hydrogen exchange rates extending as far as ±2 helical turns, pointing to a substantial degree of cooperativity in SAH stability. Whereas a single Gly substitution caused transient breaks ∼20% of the time, two consecutive Gly substitutions break the helix ∼65% of the time. NMR relaxation measurements indicate that the exchange rate between an intact and a broken helix is fast (>300,000 s-1) and that for the wild-type sequence, the finite persistence length is dominated by thermal fluctuations of backbone torsion angles and H-bond lengths, not by transient helix breaking. The double mutation D27H/E28H causes a pH-dependent fraction of helix disruption, in which the helix breakage increases from 26% at pH 7.5 to 53% at pH 5.5. The ability to modulate helical integrity by pH may enable incorporation of externally tunable dynamic components in the design of molecular machines.


Asunto(s)
Cadenas Pesadas de Miosina , Espectroscopía de Resonancia Magnética , Conformación Proteica en Hélice alfa
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