RESUMEN
Clathrin-mediated endocytosis involves the sequential assembly of more than 60 proteins at the plasma membrane. An important fraction of these proteins regulates the assembly of an actin-related protein 2/3 (Arp2/3)-branched actin network, which is essential to generate the force during membrane invagination. We performed, on wild-type (WT) yeast and mutant strains lacking putative actin crosslinkers, a side-by-side comparison of in vivo endocytic phenotypes and in vitro rigidity measurements of reconstituted actin patches. We found a clear correlation between softer actin networks and a decreased efficiency of endocytosis. Our observations support a chain-of-consequences model in which loss of actin crosslinking softens Arp2/3-branched actin networks, directly limiting the transmission of the force. Additionally, the lifetime of failed endocytic patches increases, leading to a larger number of patches and a reduced pool of polymerizable actin, which slows down actin assembly and further impairs endocytosis.
Asunto(s)
Citoesqueleto de Actina/metabolismo , Actinas/genética , Endocitosis/genética , Regulación Fúngica de la Expresión Génica , Mecanotransducción Celular , Saccharomyces cerevisiae/genética , Citoesqueleto de Actina/ultraestructura , Complejo 2-3 Proteico Relacionado con la Actina/deficiencia , Complejo 2-3 Proteico Relacionado con la Actina/genética , Actinas/metabolismo , Proteínas Adaptadoras del Transporte Vesicular/deficiencia , Proteínas Adaptadoras del Transporte Vesicular/genética , Fenómenos Biomecánicos , Clatrina/deficiencia , Clatrina/genética , Glicoproteínas de Membrana/deficiencia , Glicoproteínas de Membrana/genética , Proteínas de Microfilamentos/deficiencia , Proteínas de Microfilamentos/genética , Saccharomyces cerevisiae/metabolismo , Saccharomyces cerevisiae/ultraestructura , Proteínas de Saccharomyces cerevisiae/genéticaRESUMEN
Correction for 'Non-linear elastic properties of actin patches to partially rescue yeast endocytosis efficiency in the absence of the cross-linker Sac6' by Belbahri Reda et al., Soft Matter, 2022, 18, 1479-1488, https://doi.org/10.1039/D1SM01437D.