RESUMEN
Unlike immature neurons and the ones from the peripheral nervous system (PNS), mature neurons from the central nervous system (CNS) cannot regenerate after injury. In the past 15 years, tremendous progress has been made to identify molecules and pathways necessary for neuroprotection and/or axon regeneration after CNS injury. In most regenerative models, phosphorylated ribosomal protein S6 (p-RPS6) is up-regulated in neurons, which is often associated with an activation of the mTOR (mammalian target of rapamycin) pathway. However, the exact contribution of posttranslational modifications of this ribosomal protein in CNS regeneration remains elusive. In this study, we demonstrate that RPS6 phosphorylation is essential for PNS and CNS regeneration in mice. We show that this phosphorylation is induced during the preconditioning effect in dorsal root ganglion (DRG) neurons and that it is controlled by the p90S6 kinase RSK2. Our results reveal that RSK2 controls the preconditioning effect and that the RSK2-RPS6 axis is key for this process, as well as for PNS regeneration. Finally, we demonstrate that RSK2 promotes CNS regeneration in the dorsal column, spinal cord synaptic plasticity, and target innervation leading to functional recovery. Our data establish the critical role of RPS6 phosphorylation controlled by RSK2 in CNS regeneration and give new insights into the mechanisms related to axon growth and circuit formation after traumatic lesion.
Asunto(s)
Axones , Regeneración Nerviosa , Proteínas Quinasas S6 Ribosómicas 90-kDa , Animales , Ratones , Axones/metabolismo , Sistema Nervioso Central , Regeneración Nerviosa/fisiología , Neuronas/fisiología , Médula EspinalRESUMEN
Ribosomal RNAs (rRNAs) are main effectors of messenger RNA (mRNA) decoding, peptide-bond formation, and ribosome dynamics during translation. Ribose 2'-O-methylation (2'-O-Me) is the most abundant rRNA chemical modification, and displays a complex pattern in rRNA. 2'-O-Me was shown to be essential for accurate and efficient protein synthesis in eukaryotic cells. However, whether rRNA 2'-O-Me is an adjustable feature of the human ribosome and a means of regulating ribosome function remains to be determined. Here we challenged rRNA 2'-O-Me globally by inhibiting the rRNA methyl-transferase fibrillarin in human cells. Using RiboMethSeq, a nonbiased quantitative mapping of 2'-O-Me, we identified a repertoire of 2'-O-Me sites subjected to variation and demonstrate that functional domains of ribosomes are targets of 2'-O-Me plasticity. Using the cricket paralysis virus internal ribosome entry site element, coupled to in vitro translation, we show that the intrinsic capability of ribosomes to translate mRNAs is modulated through a 2'-O-Me pattern and not by nonribosomal actors of the translational machinery. Our data establish rRNA 2'-O-Me plasticity as a mechanism providing functional specificity to human ribosomes.
Asunto(s)
Biosíntesis de Proteínas , ARN Ribosómico/metabolismo , Proteínas Cromosómicas no Histona/genética , Proteínas Cromosómicas no Histona/metabolismo , Regulación de la Expresión Génica , Técnicas de Silenciamiento del Gen , Células HeLa , Humanos , MetilaciónRESUMEN
The ability to integrate 'omics' (i.e. transcriptomics and proteomics) is becoming increasingly important to the understanding of regulatory mechanisms. There are currently no tools available to identify differentially expressed genes (DEGs) across different 'omics' data types or multi-dimensional data including time courses. We present fCI (f-divergence Cut-out Index), a model capable of simultaneously identifying DEGs from continuous and discrete transcriptomic, proteomic and integrated proteogenomic data. We show that fCI can be used across multiple diverse sets of data and can unambiguously find genes that show functional modulation, developmental changes or misregulation. Applying fCI to several proteogenomics datasets, we identified a number of important genes that showed distinctive regulation patterns. The package fCI is available at R Bioconductor and http://software.steenlab.org/fCI/.
Asunto(s)
Biología Computacional/métodos , Perfilación de la Expresión Génica/métodos , Expresión Génica , Proteómica/métodos , Algoritmos , Análisis de Secuencia de ARN , Espectrometría de Masas en TándemRESUMEN
A formidable challenge in neural repair in the adult central nervous system (CNS) is the long distances that regenerating axons often need to travel in order to reconnect with their targets. Thus, a sustained capacity for axon regeneration is critical for achieving functional restoration. Although deletion of either phosphatase and tensin homologue (PTEN), a negative regulator of mammalian target of rapamycin (mTOR), or suppressor of cytokine signalling 3 (SOCS3), a negative regulator of Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway, in adult retinal ganglion cells (RGCs) individually promoted significant optic nerve regeneration, such regrowth tapered off around 2 weeks after the crush injury. Here we show that, remarkably, simultaneous deletion of both PTEN and SOCS3 enables robust and sustained axon regeneration. We further show that PTEN and SOCS3 regulate two independent pathways that act synergistically to promote enhanced axon regeneration. Gene expression analyses suggest that double deletion not only results in the induction of many growth-related genes, but also allows RGCs to maintain the expression of a repertoire of genes at the physiological level after injury. Our results reveal concurrent activation of mTOR and STAT3 pathways as key for sustaining long-distance axon regeneration in adult CNS, a crucial step towards functional recovery.
Asunto(s)
Axones/fisiología , Regeneración Nerviosa/fisiología , Fosfohidrolasa PTEN/deficiencia , Proteínas Supresoras de la Señalización de Citocinas/deficiencia , Animales , Axones/patología , Procesos de Crecimiento Celular/genética , Regulación de la Expresión Génica , Ratones , Ratones Endogámicos C57BL , Compresión Nerviosa , Nervio Óptico/citología , Nervio Óptico/crecimiento & desarrollo , Nervio Óptico/patología , Traumatismos del Nervio Óptico/genética , Traumatismos del Nervio Óptico/metabolismo , Traumatismos del Nervio Óptico/patología , Fosfohidrolasa PTEN/genética , Células Ganglionares de la Retina/metabolismo , Factor de Transcripción STAT3/metabolismo , Transducción de Señal , Proteína 3 Supresora de la Señalización de Citocinas , Proteínas Supresoras de la Señalización de Citocinas/genéticaRESUMEN
Developing approaches to promote the regeneration of descending supraspinal axons represents an ideal strategy for rebuilding neuronal circuits to improve functional recovery after spinal cord injury (SCI). Our previous studies demonstrated that genetic deletion of phosphatase and tensin homolog (PTEN) in mouse corticospinal neurons reactivates their regenerative capacity, resulting in significant regeneration of corticospinal tract (CST) axons after SCI. However, it is unknown whether nongenetic methods of suppressing PTEN have similar effects and how regenerating axons interact with the extrinsic environment. Herein, we show that suppressing PTEN expression with short-hairpin RNA (shRNA) promotes the regeneration of injured CST axons, and these axons form anatomical synapses in appropriate areas of the cord caudal to the lesion. Importantly, this model of increased CST regrowth enables the analysis of extrinsic regulators of CST regeneration in vivo. We find that regenerating axons avoid dense clusters of fibroblasts and macrophages in the lesion, suggesting that these cell types might be key inhibitors of axon regeneration. Furthermore, most regenerating axons cross the lesion in association with astrocytes, indicating that these cells might be important for providing a permissive bridge for axon regeneration. Lineage analysis reveals that these bridge-forming astrocytes are not derived from ependymal stem cells within the spinal cord, suggesting that they are more likely derived from a subset of mature astrocytes. Overall, this study reveals insights into the critical extrinsic and intrinsic regulators of axon regeneration and establishes shRNA as a viable means to manipulate these regulators and translate findings into other mammalian models.
Asunto(s)
Axones/metabolismo , Regeneración Nerviosa , Fosfohidrolasa PTEN/metabolismo , Tractos Piramidales/fisiopatología , ARN Interferente Pequeño/genética , Traumatismos de la Médula Espinal/metabolismo , Animales , Astrocitos/fisiología , Axones/fisiología , Procesos de Crecimiento Celular , Epéndimo/patología , Fibroblastos/fisiología , Células HEK293 , Humanos , Macrófagos/fisiología , Ratones , Ratones Endogámicos C57BL , Fosfohidrolasa PTEN/genética , Tractos Piramidales/metabolismo , Tractos Piramidales/fisiología , Traumatismos de la Médula Espinal/patología , Traumatismos de la Médula Espinal/fisiopatología , Células Madre/fisiologíaRESUMEN
In the adult mammalian central nervous system (CNS), axons fail to regenerate spontaneously after injury because of a combination of extrinsic and intrinsic factors. Despite recent advances targeting the intrinsic regenerative properties of adult neurons, the molecular mechanisms underlying axon regeneration are not fully understood. Here, we uncover a regulatory mechanism that controls the expression of key proteins involved in regeneration at the translational level. Our results show that mRNA-specific translation is critical for promoting axon regeneration. Indeed, we demonstrate that specific ribosome-interacting proteins, such as the protein Huntingtin (HTT), selectively control the translation of a specific subset of mRNAs. Moreover, modulating the expression of these translationally regulated mRNAs is crucial for promoting axon regeneration. Altogether, our findings highlight that selective translation through the customization of the translational complex is a key mechanism of axon regeneration with major implications in the development of therapeutic strategies for CNS repair.
Asunto(s)
Axones , Regeneración Nerviosa , Animales , Axones/metabolismo , Regeneración Nerviosa/genética , Sistema Nervioso Central/metabolismo , Neuronas/metabolismo , ARN Mensajero/metabolismo , Mamíferos/metabolismoRESUMEN
The ribosome is the central effector of protein synthesis, and its synthesis is intimately coordinated with that of proteins. At present, the most documented way to modulate ribosome biogenesis involves control of rDNA transcription by RNA polymerase I (RNA Pol I). Here we show that after infection of human cells with herpes simplex virus type 1 (HSV-1) the rate of ribosome biogenesis is modulated independently of RNA Pol I activity by a dramatic change in the rRNA maturation pathway. This process permits control of the ribosome biogenesis rate, giving the possibility of escaping ribosomal stress and eventually allowing assembly of specialized kinds of ribosomes.
Asunto(s)
Herpes Simple/metabolismo , Herpesvirus Humano 1/fisiología , ARN Polimerasa I/metabolismo , Ribosomas/metabolismo , Secuencia de Bases , Activación Enzimática , Células HeLa , Herpes Simple/genética , Humanos , Modelos Biológicos , ARN Polimerasa I/fisiología , Precursores del ARN/metabolismo , Procesamiento Postranscripcional del ARN/fisiología , Transducción de Señal/fisiologíaRESUMEN
In the injured adult central nervous system (CNS), activation of pro-growth molecular pathways in neurons leads to long-distance regeneration. However, most regenerative fibers display guidance defects, which prevent reinnervation and functional recovery. Therefore, the molecular characterization of the proper target regions of regenerative axons is essential to uncover the modalities of adult reinnervation. In this study, we use mass spectrometry (MS)-based quantitative proteomics to address the proteomes of major nuclei of the adult visual system. These analyses reveal that guidance-associated molecules are expressed in adult visual targets. Moreover, we show that bilateral optic nerve injury modulates the expression of specific proteins. In contrast, the expression of guidance molecules remains steady. Finally, we show that regenerative axons are able to respond to guidance cues ex vivo, suggesting that these molecules possibly interfere with brain target reinnervation in adult. Using a long-distance regeneration model, we further demonstrate that the silencing of specific guidance signaling leads to rerouting of regenerative axons in vivo. Altogether, our results suggest ways to modulate axon guidance of regenerative neurons to achieve circuit repair in adult.
Asunto(s)
Regeneración Nerviosa , Traumatismos del Nervio Óptico , Axones/metabolismo , Humanos , Regeneración Nerviosa/fisiología , Traumatismos del Nervio Óptico/metabolismo , Proteoma/metabolismo , ProteómicaRESUMEN
Nerve injury affects the neurophysiology of severed and bystander axons. In this issue of Neuron, Hsu et al. demonstrate that this early effect is cell-autonomous and driven by dSarm, independently of its NADase activity otherwise required for axon degeneration. The authors show that axon injury signal spreads to intact neurons via glial cells.
Asunto(s)
Axones , Neuronas , NAD+ Nucleosidasa , Neuroglía , Transducción de SeñalRESUMEN
Proteomics analyses of human nucleoli provided molecular bases for an understanding of the multiple functions fulfilled by these nuclear domains. However, the biological roles of about 100 of the identified proteins are unpredictable. The present study describes the functional characterization of one of these proteins, ISG20L2. We demonstrate that ISG20L2 is a 3' to 5' exoribonuclease involved in ribosome biogenesis at the level of 5.8 S rRNA maturation, more specifically in the processing of the 12 S precursor rRNA. The use of truncated forms of ISG20L2 demonstrated that its N-terminal half promotes the nucleolar localization and suggested that its C-terminal half bears the exoribonuclease activity. Identification of the binding partners of ISG20L2 confirmed its involvement in the biogenesis of the large ribosomal subunit. These results strongly support the notion that, in human, as it was demonstrated in yeast, 5.8 S rRNA maturation requires several proteins in addition to the exosome complex. Furthermore this observation greatly sustains the idea that the extremely conserved need for correctly processed rRNAs in vertebrates and yeast is achieved by close but different mechanisms.
Asunto(s)
Nucléolo Celular/enzimología , Exodesoxirribonucleasas/metabolismo , Ribosomas/metabolismo , Secuencia de Aminoácidos , Animales , Biología Computacional , Exodesoxirribonucleasas/química , Silenciador del Gen , Células HeLa , Humanos , Inmunoprecipitación , Datos de Secuencia Molecular , Proteínas Mutantes/metabolismo , Filogenia , Unión Proteica , Estructura Terciaria de Proteína , Transporte de Proteínas , Precursores del ARN/metabolismo , ARN Ribosómico/metabolismo , Proteínas Recombinantes de Fusión/metabolismo , Proteínas Ribosómicas/metabolismo , Proteínas de Saccharomyces cerevisiae/genética , Especificidad por Sustrato , VertebradosRESUMEN
In mammals, adult neurons fail to regenerate following any insult to adult central nervous system (CNS), which leads to a permanent and irreversible loss of motor and cognitive functions. For a long time, much effort has been deployed to uncover mechanisms of axon regeneration in the CNS. Even if some cases of functional recovery have been reported, there is still a discrepancy regarding the functionality of a neuronal circuit upon lesion. Today, there is a need not only to identify new molecules implicated in adult CNS axon regeneration, but also to decipher the fine molecular mechanisms associated with regeneration failure. Here, we propose to use cultures of adult retina explants to study all molecular and cellular mechanisms that occur during CNS regeneration. We show that adult retinal explant cultures have the advantages to (i) recapitulate all the features observed in vivo, including axon regeneration induced by intrinsic factors, and (ii) be an ex vivo set-up with high accessibility and many downstream applications. Thanks to several examples, we demonstrate that adult explants can be used to address many questions, such as axon guidance, growth cone formation and cytoskeleton dynamics. Using laser guided ablation of a single axon, axonal injury can be performed at a single axon level, which allows to record early and late molecular events that occur after the lesion. Our model is the ideal tool to study all molecular and cellular events that occur during CNS regeneration at a single-axon level, which is currently not doable in vivo. It is extremely valuable to address unanswered questions of neuroprotection and neuroregeneration in the context of CNS lesion and neurodegenerative diseases.
RESUMEN
Retinal ganglion cells (RGCs) drive diverse, light-evoked behaviors that range from conscious visual perception to subconscious, non-image-forming behaviors. It is thought that RGCs primarily drive these functions through the release of the excitatory neurotransmitter glutamate. We identified a subset of melanopsin-expressing intrinsically photosensitive RGCs (ipRGCs) in mice that release the inhibitory neurotransmitter γ-aminobutyric acid (GABA) at non-image-forming brain targets. GABA release from ipRGCs dampened the sensitivity of both the pupillary light reflex and circadian photoentrainment, thereby shifting the dynamic range of these behaviors to higher light levels. Our results identify an inhibitory RGC population in the retina and provide a circuit-level mechanism that contributes to the relative insensitivity of non-image-forming behaviors at low light levels.
Asunto(s)
Vías Nerviosas/fisiología , Células Ganglionares de la Retina/fisiología , Percepción Visual/fisiología , Animales , Femenino , Neuronas GABAérgicas/fisiología , Glutamato Descarboxilasa/metabolismo , Luz , Masculino , Ratones , Ratones Mutantes , Vías Nerviosas/efectos de los fármacos , Reflejo Pupilar/fisiología , Reflejo Pupilar/efectos de la radiación , Células Ganglionares de la Retina/efectos de la radiación , Opsinas de Bastones/metabolismo , Inconsciente en Psicología , Percepción Visual/efectos de la radiación , Ácido gamma-Aminobutírico/metabolismoRESUMEN
In the adult mammalian central nervous system (CNS), axonal damage often triggers neuronal cell death and glial activation, with very limited spontaneous axon regeneration. In this study, we performed optic nerve injury in adult naked mole-rats, the longest living rodent, with a maximum life span exceeding 30 years, and found that injury responses in this species are quite distinct from those in other mammalian species. In contrast to what is seen in other mammals, the majority of injured retinal ganglion cells (RGCs) survive with relatively high spontaneous axon regeneration. Furthermore, injured RGCs display activated signal transducer and activator of transcription-3 (STAT3), whereas astrocytes in the optic nerve robustly occupy and fill the lesion area days after injury. These neuron-intrinsic and -extrinsic injury responses are reminiscent of those in "cold-blooded" animals, such as fish and amphibians, suggesting that the naked mole-rat is a powerful model for exploring the mechanisms of neuronal injury responses and axon regeneration in mammals. J. Comp. Neurol. 525:380-388, 2017. © 2016 Wiley Periodicals, Inc.
Asunto(s)
Axones/fisiología , Regeneración Nerviosa/fisiología , Traumatismos del Nervio Óptico/fisiopatología , Células Ganglionares de la Retina/fisiología , Animales , Supervivencia Celular , Modelos Animales de Enfermedad , Inmunohistoquímica , Ratas Topo , RatasAsunto(s)
Antígenos de Neoplasias/fisiología , Nucléolo Celular/fisiología , Antígeno Ki-67/fisiología , Neoplasias/patología , Antígenos de Neoplasias/análisis , Biomarcadores de Tumor/análisis , Biomarcadores de Tumor/fisiología , División Celular , Nucléolo Celular/química , Regulación Neoplásica de la Expresión Génica , Humanos , Antígeno Ki-67/análisis , Neoplasias/química , Neoplasias/genética , Neoplasias/ultraestructura , ProteómicaRESUMEN
Neurons differ in their responses to injury, but the underlying mechanisms remain poorly understood. Using quantitative proteomics, we characterized the injury-triggered response from purified intact and axotomized retinal ganglion cells (RGCs). Subsequent informatics analyses revealed a network of injury-response signaling hubs. In addition to confirming known players, such as mTOR, this also identified new candidates, such as c-myc, NFκB, and Huntingtin. Similar to mTOR, c-myc has been implicated as a key regulator of anabolic metabolism and is downregulated by axotomy. Forced expression of c-myc in RGCs, either before or after injury, promotes dramatic RGC survival and axon regeneration after optic nerve injury. Finally, in contrast to RGCs, neither c-myc nor mTOR was downregulated in injured peripheral sensory neurons. Our studies suggest that c-myc and other injury-responsive pathways are critical to the intrinsic regenerative mechanisms and might represent a novel target for developing neural repair strategies in adults.
Asunto(s)
Axones/metabolismo , Regeneración Nerviosa/fisiología , Traumatismos del Nervio Óptico/metabolismo , Proteómica , Células Ganglionares de la Retina/metabolismo , Animales , Axones/patología , Axotomía/métodos , Supervivencia Celular/fisiología , Modelos Animales de Enfermedad , Ratones Endogámicos C57BL , Ratones Transgénicos , Neuronas/metabolismo , Neuronas/patología , Nervio Óptico/metabolismo , Nervio Óptico/patología , Transducción de Señal/fisiologíaRESUMEN
After axotomy, neuronal survival and growth cone re-formation are required for axon regeneration. We discovered that doublecortin-like kinases (DCLKs), members of the doublecortin (DCX) family expressed in adult retinal ganglion cells (RGCs), play critical roles in both processes, through distinct mechanisms. Overexpression of DCLK2 accelerated growth cone re-formation in vitro and enhanced the initiation and elongation of axon re-growth after optic nerve injury. These effects depended on both the microtubule (MT)-binding domain and the serine-proline-rich (S/P-rich) region of DCXs in-cis in the same molecules. While the MT-binding domain is known to stabilize MT structures, we show that the S/P-rich region prevents F-actin destabilization in injured axon stumps. Additionally, while DCXs synergize with mTOR to stimulate axon regeneration, alone they can promote neuronal survival possibly by regulating the retrograde propagation of injury signals. Multifunctional DCXs thus represent potential targets for promoting both survival and regeneration of injured neurons.
Asunto(s)
Actinas/metabolismo , Axones/metabolismo , Microtúbulos/metabolismo , Regeneración Nerviosa/genética , Proteínas Serina-Treonina Quinasas/genética , Células Ganglionares de la Retina/metabolismo , Animales , Axones/fisiología , Axotomía , Supervivencia Celular , Proteína Doblecortina , Quinasas Similares a Doblecortina , Conos de Crecimiento , Técnicas In Vitro , Ratones , Regeneración Nerviosa/fisiología , Neuronas/metabolismo , Neuronas/fisiología , Traumatismos del Nervio Óptico , Proteínas Serina-Treonina Quinasas/metabolismo , Células Ganglionares de la Retina/fisiología , Serina-Treonina Quinasas TOR/metabolismoRESUMEN
Different from physiological axon growth during development, a major limiting factor for successful axon regeneration is the poor intrinsic regenerative capacity in mature neurons in the adult mammalian central nervous system (CNS). Recent studies identified several molecular pathways, including PTEN/mTOR, Jak/STAT, DLK/JNK, providing important probes in investigating the mechanisms by which the regenerative ability is regulated. This review will summarize these recent findings and speculate their implications.
Asunto(s)
Axones/fisiología , Sistema Nervioso Central/citología , Regeneración Nerviosa/fisiología , Neuronas/citología , Transducción de Señal/fisiología , Animales , Sistema Nervioso Central/fisiología , Humanos , Quinasas Janus/metabolismo , MAP Quinasa Quinasa 4 , Neuronas/fisiología , Fosfohidrolasa PTEN/metabolismoRESUMEN
Axon regeneration capacity often declines with age. One might assume that loss of regeneration is an obvious consequence of organismal aging. However, in the latest issue of Neuron, Byrne et al. (2014) demonstrate that regeneration ability and aging are regulated cell-autonomously within neurons, and can be decoupled.