Hardware for cell culture electrical impedance tomography: A critical review.

Human cell cultures are powerful laboratory tools for biological models of diseases, drug development, and tissue engineering. However, the success of biological experiments often depends on real-time monitoring of the culture state. Conventional culture evaluation methods consist of end-point laborious techniques, not capable of real-time operation and not suitable for three-dimensional cultures. Electrical Impedance Tomography (EIT) is a non-invasive imaging technique with high potential to be used in cell culture monitoring due to its biocompatibility, non-invasiveness, high temporal resolution, compact hardware, automatic operation, and high throughput. This review approaches the different hardware strategies for cell culture EIT that are presented in the literature, discussing the main components of the measurement system: excitation circuit, voltage/current sensing, switching stage, signal specifications, electrode configurations, measurement protocols, and calibration strategies. The different approaches are qualitatively discussed and compared, and design guidelines are proposed.

Biomedical Serious Game System for Balance Rehabilitation of Hemiparetic Stroke Patients.

Hemiparetic stroke patients can have several muscular and postural disorders which compromise their balance. Serious games (SG) emerged as a new approach to enhance conventional treatment by making it a motivating method to meet individual needs. This paper evaluated the therapeutic effects of a biomedical SG system developed for balance evaluation and training of hemiparetic stroke patients. The system consists of a balance board with inertial sensors and a computer system that runs the game. A novel scoring system for balance evaluation, which extracts metric information regarding patients' performance while gaming, was proposed. A quasi-experimental study was performed with six hemiparetic patients in an exercise program using the SG twice per week for 10 weeks. Twelve healthy subjects were recruited for determining the baseline score for balance by using the proposed system. Significant effect sizes (ES) were observed for dynamic balance (ES = 0.9), functional mobility (ES = 0.4), and center of pressure displacement of the balance board (ES = 1.9).Significant correlations between game scores and clinical scales suggest that the use of the scoring system for balance evaluation is feasible. The results of this paper support the clinical potential of a biomedical SG for balance rehabilitation of hemiparetic stroke patients.

Note: Temperature effects in the modified Howland current source for electrical bioimpedance spectroscopy.

The aim of this study is to show how the modified Howland current source (MHCS) is affected by temperature changes. The source has been tested in a temperature range from 20 to 70 °C and frequency range from 100 Hz to 1 MHz. Parameters like output current, output impedance, total harmonic distortion, and oscillation have been measured. The measurements were made inside a temperature controlled environment. It was showed that the MHCS is stable at temperatures below 70 °C. Operational amplifiers with a low temperature drift and matching resistor should be carefully considered in order to prevent oscillations at high temperatures.

Howland current source for high impedance load applications.

For Electrical Impedance Spectroscopy (EIS) applications, the Enhanced Howland Current Source (EHCS) is a popular choice as an excitation circuit due to its simplicity, reliability, and safety. However, its output impedance degradation at high frequency leads to errors that are unacceptable for high load impedance applications, such as the ones which use dry or microelectrodes. Based on a proposed mathematical model, this work designed an EHCS circuit which includes an output current buffer and frequency compensation. PSpice simulations were performed as proof of concept, and then the measured data were collected for comparison. For the proposed circuit, called here Load-in-the-Loop Compensated Enhanced Howland Source (LLC-EHCS), the results showed that the output current errors are lower than 1% up to 3.7 MHz over the load range of 560-2200 Ω and 1.2 MHz with 5.6 kΩ. On the other hand, for the case of the standard EHCS circuit, these frequencies are 170 and 80 kHz, respectively. Also, the output linear swing was found to be 3 times higher than the EHCS. It can be concluded that the proposed LLC-EHCS may be widely used as an excitation circuit for high load and wide bandwidth EIS applications.

A comparison of modified Howland circuits as current generators with current mirror type circuits.

Multi-frequency electrical impedance tomography (EIT) systems require stable voltage controlled current generators that will work over a wide frequency range and with a large variation in load impedance. In this paper we compare the performance of two commonly used designs: the first is a modified Howland circuit whilst the second is based on a current mirror. The output current and the output impedance of both circuits were determined through PSPICE simulation and through measurement. Both circuits were stable over the frequency ranges 1 kHz to 1 MHz. The maximum variation of output current with frequency for the modified Howland circuit was 2.0% and for the circuit based on a current mirror 1.6%. The output impedance for both circuits was greater than 100 kohms for frequencies up to 100 kHz. However, neither circuit achieved this output impedance at 1 MHz. Comparing the results from the two circuits suggests that there is little to choose between them in terms of a practical implementation.