RESUMEN
To resist to ß-lactam antibiotics Eubacteria either constitutively synthesize a ß-lactamase or a low affinity penicillin-binding protein target, or induce its synthesis in response to the presence of antibiotic outside the cell. In Bacillus licheniformis and Staphylococcus aureus, a membrane-bound penicillin receptor (BlaR/MecR) detects the presence of ß-lactam and launches a cytoplasmic signal leading to the inactivation of BlaI/MecI repressor, and the synthesis of a ß-lactamase or a low affinity target. We identified a dipeptide, resulting from the peptidoglycan turnover and present in bacterial cytoplasm, which is able to directly bind to the BlaI/MecI repressor and to destabilize the BlaI/MecI-DNA complex. We propose a general model, in which the acylation of BlaR/MecR receptor and the cellular stress induced by the antibiotic, are both necessary to generate a cell wall-derived coactivator responsible for the expression of an inducible ß-lactam-resistance factor. The new model proposed confirms and emphasizes the role of peptidoglycan degradation fragments in bacterial cell regulation.
Asunto(s)
Bacillus/genética , Regulación Bacteriana de la Expresión Génica/genética , Oxidorreductasas actuantes sobre Donantes de Grupo CH-CH/metabolismo , Peptidoglicano/metabolismo , Resistencia betalactámica/genética , Acilación , Bacillus/enzimología , Proteínas Bacterianas/química , Proteínas Bacterianas/metabolismo , Pared Celular/química , Pared Celular/metabolismo , ADN Bacteriano/química , ADN Bacteriano/metabolismo , Dipéptidos/química , Dipéptidos/metabolismo , Inducción Enzimática/genética , Regulación Bacteriana de la Expresión Génica/efectos de los fármacos , Humanos , Metaloendopeptidasas/química , Metaloendopeptidasas/metabolismo , Oxidorreductasas actuantes sobre Donantes de Grupo CH-CH/química , Oxidorreductasas actuantes sobre Donantes de Grupo CH-CH/efectos de los fármacos , Penicilinas/metabolismo , Penicilinas/farmacología , Peptidoglicano/química , Staphylococcus aureus/enzimología , Staphylococcus aureus/genética , beta-Lactamasas/biosíntesis , beta-Lactamasas/genéticaRESUMEN
In Bacillus licheniformis 749/I, BlaP ß-lactamase is induced by the presence of a ß-lactam antibiotic outside the cell. The first step in the induction mechanism is the detection of the antibiotic by the membrane-bound penicillin receptor BlaR1 that is composed of two functional domains: a carboxy-terminal domain exposed outside the cell, which acts as a penicillin sensor, and an amino-terminal domain anchored to the cytoplasmic membrane, which works as a transducer-transmitter. The acylation of BlaR1 sensor domain by the antibiotic generates an intramolecular signal that leads to the activation of the L3 cytoplasmic loop of the transmitter by a single-point cleavage. The exact mechanism of L3 activation and the nature of the secondary cytoplasmic signal launched by the activated transmitter remain unknown. However, these two events seem to be linked to the presence of a HEXXH zinc binding motif of neutral zinc metallopeptidases. By different experimental approaches, we demonstrated that the L3 loop binds zinc ion, belongs to Gluzincin metallopeptidase superfamily and is activated by self-proteolysis.