RESUMEN
Tumor resistance is the main cause of treatment failure and is associated with many tumor factors. Jaridon 6, a new diterpene extracted from Rabdosia rubescens (Hemsl.) Hara, which has been previously extracted by our research team, has been tested having more obvious advantages in resistant tumor cells. However, its mechanism is unclear. In this study, we studied the effect and the specific mechanism of Jaridon 6 in resistant gastric cancer cells. Cytotoxicity test, colony test, western blotting, and nude test verified the anti-drug resistance ability of Jaridon 6 in the MGC803/PTX and MGC803/5-Fu cells. Jaridon 6 has shown obvious inhibitory effects in the sirtuin 1 (SIRT1) enzyme test. Transmission electron microscopy and immunofluorescence tests further proved the autophagic action of Jaridon 6. Jaridon 6 could inhibit the proliferation of the resistant gastric cancer cell in vivo and in vitro. Jaridon 6 inhibited SIRT1 enzyme and induced autophagy by inhibiting the phosphoinositide 3-kinase/protein kinase B (PI3K/AKT) pathway. Thus, it may be considered for treating gastric cancer resistance by individual or combined administration, as an SIRT1 inhibitor and autophagy inducer.
Asunto(s)
Diterpenos de Tipo Kaurano , Isodon , Neoplasias Gástricas , Apoptosis , Autofagia , Línea Celular Tumoral , Proliferación Celular , Humanos , Fosfatidilinositol 3-Quinasas , Proteínas Proto-Oncogénicas c-akt , Sirtuina 1 , Neoplasias Gástricas/tratamiento farmacológicoRESUMEN
The main aim of this study was to establish a novel paclitaxel (PTX)-resistant human gastric carcinoma cell line and to investigate its biological significance. A cell line, MGC803/PTX, was established by gradually increasing PTX density on the basis of MGC803 over a period of 10 months. In addition, a pair of resistant cell lines (SW620 and SW620/PTX) were added to further explain the resistant mechanism of PTX. The drug resistance index and stability of MGC803/PTX cells were detected using the Cell Counting Kit-8 method. The morphological features were observed using inverted microscopy. Apoptosis was measured by flow cytometry (FCM) and Hoechst 33258 fluorescence staining. The distribution of the cell cycle was determined by FCM, and protein expressions of P-gp, Bcl-2, Bax, and PARP were detected by western blot analysis. When characterizing the resistance in vitro, we found that MGC803/PTX cells were 10.3-fold more resistant to PTX compared with MGC803 cells. In addition, MGC803/PTX cells showed cross-resistance to 5-fluorouracil and adriamycin. FCM and Hoechst 33258 fluorescence staining indicated that MGC803/PTX cells had a significantly lower percentage of apoptotic cells after treatment with PTX compared with MGC803 cells. Other differences between parental cells and resistant cells included morphology, proliferation rate, doubling time, cell cycle distribution, and colony-formation rate. Western blot analysis indicated that P-gp, Bcl-2, and PARP protein were more abundant in MGC803/PTX and SW620/PTX cells compared with MGC803 and SW620 cells, whereas Bax protein levels were lower in resistant cells. Furthermore, MGC803/PTX cells showed obvious resistance to PTX in vivo. To our knowledge, this is the first report on the establishment of a PTX-resistant MGC803 cell line, which is an important tool to explore the resistance of anticancer drugs and to overcome tumor drug resistance.
Asunto(s)
Línea Celular Tumoral , Paclitaxel/farmacología , Neoplasias Gástricas/tratamiento farmacológico , Neoplasias Gástricas/patología , Animales , Antineoplásicos Fitogénicos/farmacología , Apoptosis/efectos de los fármacos , Apoptosis/fisiología , Procesos de Crecimiento Celular/efectos de los fármacos , Procesos de Crecimiento Celular/fisiología , Resistencia a Antineoplásicos , Femenino , Humanos , Ratones , Ratones Endogámicos BALB C , Fenotipo , Distribución Aleatoria , Ensayos Antitumor por Modelo de XenoinjertoRESUMEN
A new C(18)-diterpenoid alkaloid, kirinenine A (1), was isolated from the root of Aconitum kirinense, along with eight known diterpenoid alkaloids. The structures of all compounds were characterized on the basis of extensive NMR and MS analyses and by comparison with literature values, and the new one was further confirmed by X-ray crystallographic diffraction. All the compounds were isolated from the title plant for the first time.
Asunto(s)
Aconitum/química , Alcaloides/aislamiento & purificación , Diterpenos/aislamiento & purificación , Medicamentos Herbarios Chinos/aislamiento & purificación , Aconitina/análogos & derivados , Alcaloides/química , Cristalografía por Rayos X , Diterpenos/química , Medicamentos Herbarios Chinos/química , Conformación Molecular , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Raíces de Plantas/químicaRESUMEN
Two new monoterpenes, p-mentha-1(7),8-dien-2-O-ß-D-glucoside and trans-2,4-dihydroxy-2,4-dimethyl-trans-1-acetic acid γ-lactone were isolated from the fruits of Illicium lanceolatum along with trans-2,4-dihydroxy-2,4-dimethyl-cis-1-acetic acid γ-lactone, (1R,2R,4R)-8-p-menthen-1,2-diol, trans-sobrerol, (1S,2S,4R)-p-menthane-1,2,8-triol and (1S, 2S, 4R, 8R)-p-menthane-1,2,9-triol. The structures of the isolates were confirmed by spectroscopic analysis and they showed no inhibitory effects on the in vitro growth of microbial organisms (Escherichia coli, Staphyloccocus aureus, Bacillus subtilis) at less than 1.0 mg/mL.
Asunto(s)
Frutas/química , Illicium/química , Monoterpenos/química , Extractos Vegetales/química , Antibacterianos/química , Antibacterianos/aislamiento & purificación , Antibacterianos/farmacología , Bacillus subtilis/efectos de los fármacos , Escherichia coli/efectos de los fármacos , Espectroscopía de Resonancia Magnética , Pruebas de Sensibilidad Microbiana , Modelos Químicos , Modelos Moleculares , Conformación Molecular , Peso Molecular , Monoterpenos/aislamiento & purificación , Extractos Vegetales/aislamiento & purificación , Extractos Vegetales/farmacología , Espectrometría de Masa por Ionización de Electrospray , Staphylococcus aureus/efectos de los fármacosRESUMEN
Two benzophenone glucopyranosides have been isolated from the nut shell part of Mahkota Dewa. The structures were identified as 2,4',6-trihydroxy-4-methoxy-benzophenone-2-O-ß-d-glucoside (Mahkoside A) and 2,4',6-trihydroxy-4-methoxy-6â³-acetyl-benzophenone-2-O-ß-d-glucoside (Mahkoside B). Mahkoside B was recognized as a novel compound. Furthermore, a series of benzophenone glucopyranoside derivatives (compounds 3-18) were synthesized and their bioactivities were characterized. Our results demonstrated that compound 18 has significant cytotoxicity against two esophageal cancer cell lines, stomach cancer cell line and prostate cancer cell line, with IC(50) less than 10 µM, indicating its potential activity against cancer cells.
Asunto(s)
Benzofenonas/química , Glucósidos/química , Glicósidos/química , Fenoles/química , Thymelaeaceae/química , Benzofenonas/aislamiento & purificación , Benzofenonas/toxicidad , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Ensayos de Selección de Medicamentos Antitumorales , Glucósidos/síntesis química , Glucósidos/aislamiento & purificación , Glucósidos/toxicidad , Glicósidos/aislamiento & purificación , Glicósidos/toxicidad , Humanos , Nueces/química , Fenoles/aislamiento & purificación , Fenoles/toxicidad , Especies Reactivas de Oxígeno/metabolismoRESUMEN
A new series of tanshinone IIA (DIIA) derivatives were synthesized through the reaction of brominated tanshinone IIA (1-Br DIIA) and aromatic acids in the presence of K(2)CO(3). Twenty compounds were synthesized, and all of them were novel. Vasodilative activities for synthesized compounds were valuated in vitro on the contractile response of vascular thoracic aorta smooth muscle from Wistar rats. The results showed that most compounds exhibited a concentration-dependent inhibition on the contractile response of norepinephrine. Four prepared compounds, 4, 5, 8 and 13 revealed relatively remarkable vasodilative activity.
Asunto(s)
Abietanos/química , Diseño de Fármacos , Vasodilatadores/síntesis química , Animales , Aorta Torácica/efectos de los fármacos , Masculino , Contracción Muscular/efectos de los fármacos , Norepinefrina/farmacología , Ratas , Ratas Wistar , Relación Estructura-Actividad , Vasodilatadores/química , Vasodilatadores/farmacologíaRESUMEN
A series of 2,2'-(substituted methylene)bis-(1,6,6-trimethyl-6,7,8,9-tetrahydrophenanthro[1,2-b]furan-10,11-dione) derivatives were synthesized by the reaction of tanshinone IIA (D(1)) and aromatic aldehyde in the presence of p-TsOH. Bromination derivative of D(1) and hydrolysis product of cryptotanshinone (D(2)) were also prepared in this work. Vasodilation activity in vitro of them was valuated on the contractile response of vascular thoracic aorta smooth muscle from Wistar rats for the first time. Most of them exhibited a concentration-dependent inhibition on the contractile response of norepinephrine.
Asunto(s)
Fenantrenos/química , Vasodilatadores/síntesis química , Abietanos , Animales , Aorta Torácica/efectos de los fármacos , Músculo Liso Vascular/efectos de los fármacos , Norepinefrina/metabolismo , Fenantrenos/síntesis química , Fenantrenos/farmacología , Ratas , Ratas Wistar , Salvia miltiorrhiza/química , Relación Estructura-Actividad , Vasodilatadores/química , Vasodilatadores/farmacologíaRESUMEN
Two new compounds 1 and 2 have been isolated from the aerial parts of Urena lobata L. The structures of the two new compounds were established as ceplignan-4-O-ß-d-glucoside (1) and 2,5-dihydroxy benzoic acid-7-(2,6-dimethyl-6-hydroxy-2,7-octadienoic acid) anhydride-5-O-ß-d-apiofuranosyl(1 â 2)-ß-d-glucoside (urenoside A) (2), on the basis of chemical and spectral evidence, including 1D and 2D NMR spectroscopic data as well as mass spectrometry (HR-ESI-MS).
Asunto(s)
Medicamentos Herbarios Chinos/aislamiento & purificación , Glicósidos/aislamiento & purificación , Lignanos/aislamiento & purificación , Malvaceae/química , Plantas Medicinales/química , Medicamentos Herbarios Chinos/química , Flavonas , Glucósidos , Glicósidos/química , Lignanos/química , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , EstereoisomerismoRESUMEN
Objective: To make full usage of resource and turn waste into treasure, the chemical constituents and bioactivity were firstly investigated on Damask rose (Rosa damascena) flower residue (DRFR). Methods: DPPH and ABTS experiments were applied to assess the antioxidant activity of DRFR. Then, column chromatography was used to purify compounds from an antioxidation extract (DRFR-A), and the chemical structure was identified using NMR. The total phenolic acid content was measured by Folin-Ciocalteu colorimetric method, and the content of gallic acid of the indicator ingredient was detected by HPLC. Results: DRFR-A was found to show a high activity both on DPPH (IC50: 2.760 µg/mL) and ABTS (IC50: 2.258 µg/mL) compared to positive control VC. Ten compounds were isolated and identified as quercetin (1), kaempferol (2), gallic acid (3), protocatechuic acid (4), pyrogallic acid (5), 2-phenylethyl 3,4,5-trihydroxybenzoate (6), methyl gallate (7), p-hydroxybenzoic acid (8), p-hydroxyphenethyl alcohol (9) and astragalin (10) from DRFR-A. Among them, pyrogallic acid, 2-phenylethyl-3, 4, 5-trihydroxybenzoate, p-hydroxybenzoic acid and p-hydroxyphenethyl alcohol are obtained from the plant for the first time. The content of total phenolic acids and gallic acid, main ingredient in DRFR-A was determined as 63.73% and 24.67%, respectively. Conclusion: This study provides a reliable data and lays the foundation for the development and utilization of rose residue, and hence for the full utilization of rose resources.
RESUMEN
AIM: To study the chemical constituents of the water-extracts of the pine needles of Pinus massoniana Lamb.. METHODS: Pine needles were collected in Xixia country and extracted with boiling water for two times and the water-extracts were concentrated. The crude extract of pine needles was fractionated into four fractions by Et2O, EtOAc and n-BuOH. Various column chromatography with D-101 macroreticular resin, Toyopearl HW-40 and silica gel were employed for the isolation and purification of compounds from the n-BuOH fraction of pine needles. The structures of the compounds were identified by physiochemical properties and spectral analysis (UV, IR, MS, 1HNMR, 13CNMR, DEPT, HMQC, HMBC, etc.). RESULTS: Four compounds were isolated from the n-BuOH fraction of water-extracts, and their structures were identified as 3-methoxyl-9'-O-alpha-L-rhamnopyranosyl-4':7,5':8-diepoxyneoligan-4,9-diol (massonianoside E, I), 4,4',8,8',9-pentahydroxyl-3,3'-dimethoxyl-7,9'-monoepoxylignan (II), umbelliferon (III), 4-(4'-hydroxyl-3'-methoxylbenzyl)-2-butanone (IV). CONCLUSION: Compound I is a new compound, and compounds II, III and IV were isolated from this plant for the first time.
Asunto(s)
Lignanos/aislamiento & purificación , Pinus/química , Plantas Medicinales/química , Lignanos/química , Conformación Molecular , Estructura Molecular , Hojas de la Planta/química , Umbeliferonas/química , Umbeliferonas/aislamiento & purificaciónRESUMEN
AIM: To study the chemical constituents of the water-extracts of Selaginella tamariscina (Beauv.) Spring. METHODS: Various chromatographic techniques were used to separate and purify the constituents. Their physico-chemical properties and spectral data were used to elucidate the structures. RESULTS: Nine compounds were isolated and identified as (2R,3S)-dihydro-2- (3',5'-dimethoxy-4'-hydroxyphenyl)-7-methoxy-5-acetyl-benzofuran (1), 3-hydroxy-phenpropionic acid-(2'-methoxy-4'-carboxy-phenol) ester (tamariscina ester A, 2), sygringaresinol (3), 1-(4'-hydroxyl-3'-methoxyphenyl)glycerol (4), ferulic acid (5), caffeic acid (6), vanillic acid (7), syringic acid (8), and umbelliferone (9). CONCLUSION: Compound 1 and 2 are new compounds, and the others were isolated from Selaginella for the first time.
Asunto(s)
Benzofuranos/aislamiento & purificación , Fenilpropionatos/aislamiento & purificación , Plantas Medicinales/química , Selaginellaceae/química , Benzofuranos/química , Ácidos Cafeicos/química , Ácidos Cafeicos/aislamiento & purificación , Ácidos Cumáricos/química , Ácidos Cumáricos/aislamiento & purificación , Conformación Molecular , Estructura Molecular , Fenilpropionatos/química , Ácido Vanílico/química , Ácido Vanílico/aislamiento & purificaciónRESUMEN
AIM: To study the chemical constituents of the pine needles from Pinus massoniana Lamb.. METHODS: Various chromatographic techniques were used for the separation and purification. The physicochemical properties and spectral data were used to elucidate the structures. RESULTS: Three compounds were isolated from the n-BuOH fraction of water-extracts. The structures were identified as (7S,8R)-4,9'-dihydroxyl-3, 3'-dimethyoxyl-7, 8-dihydrobenzofunan-1'-propanolneolignan-9-O-alpha- L-rhamnopyranoside (massonianoside D, 7), 4,4',9,9'-tetrahydroxyl-3,3'-dimethyoxyl-tetrahydrocyclolignan ((+)-isolariciresinol, 8) and 4,4',9'-trihydroxyl-3,3'-dimethyoxyl-tetrahydrocyclolignan-9-O-beta-D- xylopyranoside (isolariciresinol-9-O-xyloside, 9) on the basis of spectral data (ORD, UV, IR, MS, 1HNMR, 13CNMR, 1H-1HCOSY, HMQC and HMBC etc.). CONCLUSION: Compound 7 is a new compound, while compounds 8 and 9 were isolated from this plant for the first time.
Asunto(s)
Lignanos/aislamiento & purificación , Monosacáridos/aislamiento & purificación , Pinus/química , Plantas Medicinales/química , Medicamentos Herbarios Chinos/química , Medicamentos Herbarios Chinos/aislamiento & purificación , Lignanos/química , Lignanos/clasificación , Lignina/química , Lignina/aislamiento & purificación , Estructura Molecular , Monosacáridos/química , Naftoles/química , Naftoles/aislamiento & purificación , Hojas de la Planta/químicaRESUMEN
AIM: To study the chemical constituents from Corallodiscus flabellata. METHODS: Fresh plant of Corallodiscus flabellata was extracted twice with boiling water, filtered to remove insoluble materials, concentrated under reduced pressure at temperature 55 degrees C to a small volume. The concentrated liquor was subjected to solvent-solvent partitioning using ether, ethyl acetate, and n-butanol (saturated with water). The fraction of ethyl acetate extract was chromatographed over macroporous adsorption resin (Diaion HP-20) eluted with a mixture of H2O and MeOH in increasing MeOH content. Their fractions from resin were repeatedly chromatographed over Sephadex LH-20, Toyopearl HW-40, gel MCI, Gel CHP-20 and silica gel column. Structures of compounds obtained were identified on the basis of their spectral data, hydrolysis and chemical correlation. RESULTS: Two phenylethanoid glycosides (I, II) and three phenolic acids were obtained from the EtOAc fraction of water-extracts. Their structures were identified as 3,4-dihydroxyphenylethanol-8-O-[beta-D-apiofuranosyl (1-->2)]-beta-D-glucopyranoside (I), 3,4-dihydroxyphenylethanol-8-O-[(5-O- Vanilloyl)-beta-D-apiofuranosyl(1-->2)]-beta-D-glucopyranoside (II), vanillic acid (III), syringic acid (IV) and ferulic acid (V). CONCLUSION: I and II are new compounds. Compounds III, IV and V were isolated from this plant for the first time.
Asunto(s)
Disacáridos/aislamiento & purificación , Magnoliopsida/química , Plantas Medicinales/química , Disacáridos/química , Medicamentos Herbarios Chinos/química , Estructura Molecular , Ácido Vanílico/química , Ácido Vanílico/aislamiento & purificaciónRESUMEN
AIM: To study the chemical constituents of the pine needles of Pinus massoniana lamb.. METHODS: Various chromatographic techniques were used to separate and purify. Their physico-chemical properties and spectral data (UV, IR, MS, 1H-1 H COSY, HMQC, DEPT, HMBC and ORD ect.) were measured for structure elucication. RESULTS: Three compounds were isolated from the n-BuOH fraction of water-extracts. Their structures were identified as massonianoside A (4), massonianoside A: (7S, 8R)-3, 4, 9'-trihydroxyl-3-methyoxyl-7, 8-dihydrobenzofunan-1'-propanolneoligan-9-O-alpha-L-rhamnopyranoside, massonianoside C (5), (7S, 8R)-9,9'-dihydroxyl-3,3'-dimethyoxyl-7,8-dihydrobenzofunan-1'- propanolneoligan-4-O-alpha-L-rhamnopyranoside and cedrusin-4-O-beta-glucoside (6), (7S, 8R)-3',9,9'-trihydroxyl-3-methoxyl-7,8-dihydrobenzofunan-1'- propanolneoligan-4-O-beta-D-glucopyranoside. CONCLUSION: Compound 4 and 5 are new compounds.
Asunto(s)
Benzofuranos/aislamiento & purificación , Glicósidos/aislamiento & purificación , Pinus/química , Plantas Medicinales/química , Benzofuranos/química , Glicósidos/química , Estructura Molecular , Hojas de la Planta/químicaRESUMEN
AIM: To study the chemical constituents from Corallodiscus flabellata. METHODS: The compounds were isolated and purified by macroporous adsorption resin, silica gel column chromatography and identified on the basis of their physiochemical and spectral data. RESULTS: Three phenylethanoid glycosides (I-III) were obtained from the n-BuOH fraction of water-extracts. Their structures were elucidated as 3,4-dihydroxyphenylethanol-8-O-[beta-D-apiofuranosyl (1-->3)]-beta-D-glucopyranoside (I), 3,4-dihydroxyphenylethanol-8-O-[4-O-trans-caffeoyl-beta-D-apiofuranosyl (1-->3)-beta-D-glucopyranosyl-(1-->6)]-beta-D-glucopyranoside (II) and 3,4-dihydroxyphenylethanol-8-O-[beta-D-apiofuranosyl(1-->3)-beta- D-glucopyranosyl-(1-->6)]-beta-D-glucopyranoside (III). CONCLUSION: Compounds I, II and III are new compounds.
Asunto(s)
Ácidos Cafeicos/aislamiento & purificación , Disacáridos/aislamiento & purificación , Glicósidos/aislamiento & purificación , Magnoliopsida/química , Ácidos Cafeicos/química , Disacáridos/química , Glicósidos/química , Estructura Molecular , Plantas Medicinales/químicaRESUMEN
AIM: To study the chemical constituents from the water-extracts of pine needles of Pinus massoniana Lamb. METHODS: Chromatographic techniques were used to separate and purify compounds. Their physico-chemical properties and spectral data (UV, IR, MS, 1H-1H, 13C-1H NMR, DEPT, HMBC etc.) were used to elucidate the structures. RESULTS: Three lignans were isolated from the n-BuOH fraction of water-extracts. Their structures were identified as (7S,8R)-3',4,9'-tridihydroxy-4-methoxy- 9-O-shikimoyl-7,8-dihydrobenzofuran-1'-propylneolignan (massonianoid A, I), (7S,8R)-4,9-dihydroxy-3,3'-dimethoxy-7,8- dihydrobenzofuran-1'-propylneolignan (II) and 4,4',8-trihydroxy-4,4'-dimethoxy-9-lignanolide (III). CONCLUSION: Compound I is a new compound. While II and III were isolated from this plant for the first time.
Asunto(s)
Lignanos/aislamiento & purificación , Pinus/química , Plantas Medicinales/química , Furanos/química , Furanos/aislamiento & purificación , Lignanos/química , Estructura Molecular , Hojas de la Planta/químicaRESUMEN
AIM: To study the chemical constituents of Selaginella tamariscina (Beauv.) Spring. METHODS: Various chromatographic techniques were used to separate and purify the chemical constituents. Their physico-chemical properties and spectral data were used to elucidate the structures. RESULTS: Four compounds were isolated from the n-BuOH fraction of the water-extracts. Their structures were identified as 1-hydroxy-2-[2-hydroxy-3-methoxy-5-(1-hydroxyethyl)-phenyl]-3-(4-hydroxy-3,5-dimethoxy)-propane-1-O-beta-D-glucopyranoside (tamariscinoside B, I), adenosine (II), guanosine (III), arbutin (IV). CONCLUSION: Tamariscinoside B (I) is a new compound, while the others were isolated from Selaginella for the first time.
Asunto(s)
Glucósidos/aislamiento & purificación , Plantas Medicinales/química , Selaginellaceae/química , Adenosina/química , Adenosina/aislamiento & purificación , Arbutina/química , Arbutina/aislamiento & purificación , Glucósidos/química , Guanosina/química , Guanosina/aislamiento & purificación , Conformación Molecular , Estructura MolecularRESUMEN
AIM: To study the chemical constituents of Selaginella tamariscina (Beauv.) Spring. METHODS: The compounds were isolated and purified by macroporous adsorption resin, Sephadex LH-20 and silica gel column chromatography and identified on the basis of their physicochemical and spectral data. RESULTS: Four compounds were obtained from the n-BuOH fraction of 70% acetone extracts. Their structures were elucidated as (7S, 8R)-7, 8-dihydro-7-(4-hydroxy-3,5-dimethoxyphenyl)-8-hydroxymethyl-[1'-( 7'-hydroxyethyl)-5' methoxyl] benzofuran-4-O-beta-D-glucopyranoside (tamariscinoside C, I), D-mannitol (II), tyrosine (II), shikimic acid (IV). CONCLUSION: Compound I is a new compound, compounds II and III were obtained from the genius for the first time, compound IV was yielded from the plant for the first time.
Asunto(s)
Benzofuranos/aislamiento & purificación , Monosacáridos/aislamiento & purificación , Plantas Medicinales/química , Selaginellaceae/química , Benzofuranos/química , Manitol/química , Manitol/aislamiento & purificación , Conformación Molecular , Estructura Molecular , Monosacáridos/química , Ácido Shikímico/química , Ácido Shikímico/aislamiento & purificación , Tirosina/química , Tirosina/aislamiento & purificaciónRESUMEN
OBJECTIVE: To study the chemical constituents from Corallodiscus flabellata. METHOD: The compounds were isolated with macroporous adsorption resin, silica gel column chromatography and identified on the basis of their physiochemical and spectral data. RESULT: Six compounds were obtained and identified as vanillic acid, 3,4-dihydroxyphenylethyl-8-O-beta-D-glucopyranoside, syringic acid, caffeic acid, isoacteoside, ferulic acid. CONCLUSION: All the compounds were isolated from this plant for the first time.
Asunto(s)
Ácido Gálico/análogos & derivados , Ácido Gálico/aislamiento & purificación , Glucósidos/aislamiento & purificación , Magnoliopsida/química , Fenoles/aislamiento & purificación , Plantas Medicinales/química , Ácido Vanílico/aislamiento & purificación , Ácido Gálico/química , Glucósidos/química , Fenoles/química , Ácido Vanílico/químicaRESUMEN
AIM: To study the chemical constituents of the fruits of Illicium henryi. METHOD: Chromatographic separations on silica gel, Sephadex LH-20 gel and MCI gel were used to isolate the compounds. The structures were elucidated based on extensive spectroscopic data analyses. RESULTS: Seven compounds were obtained and their structures were identified as 10-benzoyl-cycloparvifloralone (1), cycloparvifloralone (2), 2α-hydroxycycloparviforalone (3), henrylactone B (4), merrillianone (5), henrylactone C (6) and 7, 14-ortholactone- 3-hydroxyfloridanolide (7). CONCLUSION: Compound 1 is a new sesquiterpene lactone. The tested compounds showed weak anti-HBV activities on HBV surface antigen (HBsAg) secretion and HBV e antigen (HBeAg) secretion using Hep G2.2.15 cell line.