Diagnosis of Dermatophytosis by Conventional Methods and Comparatative analysis of Sabouraud Dextrose Agar and Dermatophyte Test Medium for Isolation of Dermatophytes.

Dermatophytosis is a fungal disease which ranked as one of the most common cutaneous condition in all over the world. The aim of this cross sectional observational study is to identify the etiological agent of dermatophytosis and comparison of Sabouraud dextrose agar (SDA) and Dermatophyte test medium (DTM) for the primary isolation of dermatophytes among the clinically suspected cases of dermatophytosis from the outpatient Department of Dermatology and Venereology, Mymensingh Medical College Hospital from July 2013 to December 2015. Two hundred thirty clinically suspected cases of dermatophytosis were subjected in this study. Sixty three cases (27.4%) were positive for fungus in direct microscopy while 53(23%) were culture positive. Trichophyton rubrum (83%) was found common etiological dermatophyte species followed by Trichophyton mentagrophytes 5(9.4%) and Epidermophyton floccosum 4(7.5%). There was no statistically significant difference between the SDA and DTM (p<0.01) in primary isolation of dermatophytes.

Invitro Activity of Four Commonly Available Antifungal Drugs against Dermatophytes Species Isolated From Clinical Samples Using Disk Diffusion Method in Mymensingh.

Dermatophytosis is an infection with fungi related to the skin, hair and/or nails. Fungal infections' treatment orally in dermatology has become a superior modality for the management of these conditions. In spite of increasing the number of antifungal drugs, treatment failure has been noticed against some cases as well as some relapse cases. To eradicate dermatophyte it is necessary to perform in vitro antifungal susceptibility testing of dermatophyte. Simple disk diffusion method in vitro assay may be used to assess antifungal propensity in dermatophytes. This cross sectional study was aimed to evaluate the antifungal activity of four antifungal drugs against several fresh clinical dermatophyte isolates by disk diffusion method at the out patient department with the collaboration of Microbiology department, Mymensingh Medical College, Mymensingh, Bangladesh from July 2013 to September 2014. Fifty three clinical dermatophytes species were isolated from patients suspected of having active dermatophytosis. Terbinafine, griseofulvin, fluconazole and itraconazole containing paper disks were used in disk diffusion method for evaluation in vitro activity of antifungal agents by measuring the mean diameter of inhibition around the disks. The isolates belong to three genera and three species as: Trichophyton Rubrum 44(83.04%), T. mentagrophytes 5(9.43%), Epidermophyton floccosum 4(7.55%). All isolates were significant to terbinafine. Terbinafine, itraconazole and griseofulvin were exposed in this study as the most ideal antifungal drugs for the treatment of dermatophytosis. For the detection of antifungal susceptibility of dermatophytes disk diffusion method is not only a simple but also a valuable method.