Seasonal and spatial variations of greenhouse gas (CO2, CH4 and N2O) emissions from urban ponds in Brussels.

Freshwaters have been recognized as important sources of greenhouse gases (GHG) to the atmosphere. However, urban ponds have received little attention even though their number is increasing due to expanding urbanisation globally. Ponds are frequently associated to urban green spaces that provide several ecosystemic services such as cooling local climate, regulating the water cycle, and acting as small carbon sinks This study aims to identify and understand the processes producing GHGs (CO2, CH4, and N2O) in the urban ponds of the temperate European city of Brussels in Belgium. 22 relatively small ponds (0.1-4.6 ha) surrounded by contrasted landscape (strictly urban, bordered by cropland or by forest), were sampled during four seasons in 2021-2022. The mean ± standard deviation was 3,667 ± 2,904 ppm for the partial pressure of CO2 (pCO2), 2,833 ± 4,178 nmol L-1 for CH4, and 273 ± 662% for N2O saturation level (%N2O). Relationships of GHGs with oxygen and water temperature suggest that biological processes controlled pCO2, CH4 concentration and%N2O. However, pCO2 was also controlled by external inputs as indicated by the higher values of pCO2 in the smaller ponds, more subject to external inputs than larger ones. The opposite was observed for CH4 concentration that was higher in larger ponds, closer to the forest in the city periphery, and with higher macrophyte cover. N2O concentrations, as well as dissolved inorganic nitrogen, were higher closer to the city center, where atmospheric nitrogen deposition was potentially higher. The total GHG emissions from the Brussels ponds were estimated to 1kT CO2-eq per year and were equivalent to the carbon sink of urban green spaces.

Determination of dimethylsulfoniopropionate and dimethylsulfoxide in Posidonia oceanica leaf tissue.

In order to investigate the possible use of the dimethylsulfoniopropionate (DMSP) and dimethylsulfoxide (DMSO) ratio as a stress indicator of Posidonia oceanica a method for the determination of these quantities was developed for this type of material. •The method relies on gas chromatography with headspace technique, instead of the purge-and-trap technique commonly used.•The method allows the determination of both DMSP and DMSO on the same sample.•This method allows to quantify DMSP, DMSO and DMSP:DMSO ratio for calibration curves with a coefficient of variation around 2% and a relative error around 2% and within the ranges natural variability of DMSP and DMSO in P. oceanica leaf tissue. Preliminary tests showed that DMSP in P. oceanica leaf tissue ranged from 20 to 200 µmol g-1 of fresh weight (FW) and 2 to 5 µmol gfw -1 for DMSO. The DMSP:DMSO ratio ranged from 2 to 40. The quantifications were conducted with different mixtures of DMSP and DMSO by measurements of DMSP and DMSO in the same sample of P. oceanica leaf tissue.

Effects of agricultural land use on fluvial carbon dioxide, methane and nitrous oxide concentrations in a large European river, the Meuse (Belgium).

We report a data-set of CO2, CH4, and N2O concentrations in the surface waters of the Meuse river network in Belgium, obtained during four surveys covering 50 stations (summer 2013 and late winter 2013, 2014 and 2015), from yearly cycles in four rivers of variable size and catchment land cover, and from 111 groundwater samples. Surface waters of the Meuse river network were over-saturated in CO2, CH4, N2O with respect to atmospheric equilibrium, acting as sources of these greenhouse gases to the atmosphere, although the dissolved gases also showed marked seasonal and spatial variations. Seasonal variations were related to changes in freshwater discharge following the hydrological cycle, with highest concentrations of CO2, CH4, N2O during low water owing to a longer water residence time and lower currents (i.e. lower gas transfer velocities), both contributing to the accumulation of gases in the water column, combined with higher temperatures favourable to microbial processes. Inter-annual differences of discharge also led to differences in CH4 and N2O that were higher in years with prolonged low water periods. Spatial variations were mostly due to differences in land cover over the catchments, with systems dominated by agriculture (croplands and pastures) having higher CO2, CH4, N2O levels than forested systems. This seemed to be related to higher levels of dissolved and particulate organic matter, as well as dissolved inorganic nitrogen in agriculture dominated systems compared to forested ones. Groundwater had very low CH4 concentrations in the shallow and unconfined aquifers (mostly fractured limestones) of the Meuse basin, hence, should not contribute significantly to the high CH4 levels in surface riverine waters. Owing to high dissolved concentrations, groundwater could potentially transfer important quantities of CO2 and N2O to surface waters of the Meuse basin, although this hypothesis remains to be tested.

Establishing research strategies, methodologies and technologies to link genomics and proteomics to seagrass productivity, community metabolism, and ecosystem carbon fluxes.

A complete understanding of the mechanistic basis of marine ecosystem functioning is only possible through integrative and interdisciplinary research. This enables the prediction of change and possibly the mitigation of the consequences of anthropogenic impacts. One major aim of the European Cooperation in Science and Technology (COST) Action ES0609 "Seagrasses productivity. From genes to ecosystem management," is the calibration and synthesis of various methods and the development of innovative techniques and protocols for studying seagrass ecosystems. During 10 days, 20 researchers representing a range of disciplines (molecular biology, physiology, botany, ecology, oceanography, and underwater acoustics) gathered at The Station de Recherches Sous-marines et Océanographiques (STARESO, Corsica) to study together the nearby Posidonia oceanica meadow. STARESO is located in an oligotrophic area classified as "pristine site" where environmental disturbances caused by anthropogenic pressure are exceptionally low. The healthy P. oceanica meadow, which grows in front of the research station, colonizes the sea bottom from the surface to 37 m depth. During the study, genomic and proteomic approaches were integrated with ecophysiological and physical approaches with the aim of understanding changes in seagrass productivity and metabolism at different depths and along daily cycles. In this paper we report details on the approaches utilized and we forecast the potential of the data that will come from this synergistic approach not only for P. oceanica but for seagrasses in general.

Intermetatarsal spaces: analysis with MR bursography, anatomic correlation, and histopathology in cadavers.

PURPOSE: To describe the normal magnetic resonance (MR) imaging-depicted anatomy of the intermetatarsal spaces, with emphasis on the MR imaging appearance of the intermetatarsal bursae, and to correlate the MR findings with those seen in anatomic sections and at histopathologic analysis. MATERIALS AND METHODS: Conventional radiography and pre- and postcontrast T1-weighted and fat-saturated T1-weighted spin-echo MR imaging were performed in 32 intermetatarsal spaces in eight human cadaveric feet. The cadaveric specimens were sectioned in planes corresponding to those at MR imaging for anatomic correlation. The intermetatarsal space anatomy was analyzed. Histopathologic examinations of the bursae were performed. RESULTS: The intermetatarsal spaces were located in the forefoot between two metatarsal heads, below and above the deep transverse metatarsal ligament (DTML) that separated the spaces into two levels. The superior level contained the synovial bursa, the plantar and dorsal interosseous muscles and tendons, and the collateral ligament complexes of the metatarsophalangeal joints. The inferior level contained lumbrical muscles and neurovascular bundles. The bursae extended distally to the DTML in the second and third spaces close to the neurovascular bundles and did not extend beyond the DTML in the first and fourth spaces. In the first intermetatarsal space, the bursa had a specific appearance as it coursed along the adductor hallucis tendon as a tendon sheath. Histopathologic examination of the bursae revealed a single layer of attenuated cells. CONCLUSION: MR bursography provided detailed information about the intermetatarsal anatomy, especially the intermetatarsal bursae.