Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 66
Filtrar
Más filtros

Bases de datos
País/Región como asunto
Tipo del documento
Intervalo de año de publicación
1.
Plant Cell ; 31(12): 2947-2972, 2019 12.
Artículo en Inglés | MEDLINE | ID: mdl-31628167

RESUMEN

Flowers are essential but vulnerable plant organs, exposed to pollinators and florivores; however, flower chemical defenses are rarely investigated. We show here that two clustered terpene synthase and cytochrome P450 encoding genes (TPS11 and CYP706A3) on chromosome 5 of Arabidopsis (Arabidopsis thaliana) are tightly coexpressed in floral tissues, upon anthesis and during floral bud development. TPS11 was previously reported to generate a blend of sesquiterpenes. By heterologous coexpression of TPS11 and CYP706A3 in yeast (Saccharomyces cerevisiae) and Nicotiana benthamiana, we demonstrate that CYP706A3 is active on TPS11 products and also further oxidizes its own primary oxidation products. Analysis of headspace and soluble metabolites in cyp706a3 and 35S:CYP706A3 mutants indicate that CYP706A3-mediated metabolism largely suppresses sesquiterpene and most monoterpene emissions from opening flowers, and generates terpene oxides that are retained in floral tissues. In flower buds, the combined expression of TPS11 and CYP706A3 also suppresses volatile emissions and generates soluble sesquiterpene oxides. Florivory assays with the Brassicaceae specialist Plutella xylostella demonstrate that insect larvae avoid feeding on buds expressing CYP706A3 and accumulating terpene oxides. Composition of the floral microbiome appears also to be modulated by CYP706A3 expression. TPS11 and CYP706A3 simultaneously evolved within Brassicaceae and form the most versatile functional gene cluster described in higher plants so far.plantcell;31/12/2947/FX1F1fx1.


Asunto(s)
Transferasas Alquil y Aril/metabolismo , Proteínas de Arabidopsis/metabolismo , Arabidopsis/metabolismo , Sistema Enzimático del Citocromo P-450/metabolismo , Flores/metabolismo , Terpenos/antagonistas & inhibidores , Transferasas Alquil y Aril/genética , Animales , Arabidopsis/enzimología , Arabidopsis/genética , Arabidopsis/microbiología , Proteínas de Arabidopsis/química , Proteínas de Arabidopsis/genética , Sistema Enzimático del Citocromo P-450/química , Sistema Enzimático del Citocromo P-450/genética , Flores/genética , Flores/microbiología , Expresión Génica , Larva , Microbiota , Modelos Moleculares , Simulación del Acoplamiento Molecular , Monoterpenos/metabolismo , Mariposas Nocturnas , Familia de Multigenes , Filogenia , Sesquiterpenos/metabolismo , Terpenos/química , Terpenos/metabolismo , Nicotiana/metabolismo , Levaduras/metabolismo
2.
Environ Microbiol ; 23(8): 4450-4465, 2021 08.
Artículo en Inglés | MEDLINE | ID: mdl-34121306

RESUMEN

Chloromethane (CH3 Cl) is the most abundant halogenated volatile organic compound in the atmosphere and contributes to stratospheric ozone depletion. CH3 Cl has mainly natural sources such as emissions from vegetation. In particular, ferns have been recognized as strong emitters. Mitigation of CH3 Cl to the atmosphere by methylotrophic bacteria, a global sink for this compound, is likely underestimated and remains poorly characterized. We identified and characterized CH3 Cl-degrading bacteria associated with intact and living tree fern plants of the species Cyathea australis by stable isotope probing (SIP) with 13 C-labelled CH3 Cl combined with metagenomics. Metagenome-assembled genomes (MAGs) related to Methylobacterium and Friedmanniella were identified as being involved in the degradation of CH3 Cl in the phyllosphere, i.e., the aerial parts of the tree fern, while a MAG related to Sorangium was linked to CH3 Cl degradation in the fern rhizosphere. The only known metabolic pathway for CH3 Cl degradation, via a methyltransferase system including the gene cmuA, was not detected in metagenomes or MAGs identified by SIP. Hence, a yet uncharacterized methylotrophic cmuA-independent pathway may drive CH3 Cl degradation in the investigated tree ferns.


Asunto(s)
Helechos , Cloruro de Metilo , Atmósfera , Bacterias/genética , Metiltransferasas
3.
Curr Issues Mol Biol ; 33: 149-172, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31166190

RESUMEN

Chloromethane is a halogenated volatile organic compound, produced in large quantities by terrestrial vegetation. After its release to the troposphere and transport to the stratosphere, its photolysis contributes to the degradation of stratospheric ozone. A better knowledge of chloromethane sources (production) and sinks (degradation) is a prerequisite to estimate its atmospheric budget in the context of global warming. The degradation of chloromethane by methylotrophic communities in terrestrial environments is a major underestimated chloromethane sink. Methylotrophs isolated from soils, marine environments and more recently from the phyllosphere have been grown under laboratory conditions using chloromethane as the sole carbon source. In addition to anaerobes that degrade chloromethane, the majority of cultivated strains were isolated in aerobiosis for their ability to use chloromethane as sole carbon and energy source. Among those, the Proteobacterium Methylobacterium (recently reclassified as Methylorubrum) harbours the only characterisized 'chloromethane utilization' (cmu) pathway, so far. This pathway is not representative of chloromethane-utilizing populations in the environment as cmu genes are rare in metagenomes. Recently, combined 'omics' biological approaches with chloromethane carbon and hydrogen stable isotope fractionation measurements in microcosms, indicated that microorganisms in soils and the phyllosphere (plant aerial parts) represent major sinks of chloromethane in contrast to more recently recognized microbe-inhabited environments, such as clouds. Cultivated chloromethane-degraders lacking the cmu genes display a singular isotope fractionation signature of chloromethane. Moreover, 13CH3Cl labelling of active methylotrophic communities by stable isotope probing in soils identify taxa that differ from the taxa known for chloromethane degradation. These observations suggest that new biomarkers for detecting active microbial chloromethane-utilizers in the environment are needed to assess the contribution of microorganisms to the global chloromethane cycle.


Asunto(s)
Metabolismo Energético/fisiología , Metanol/metabolismo , Cloruro de Metilo/metabolismo , Proteobacteria/clasificación , Proteobacteria/metabolismo , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Biodegradación Ambiental , Sedimentos Geológicos/microbiología , Redes y Vías Metabólicas/genética , Methylobacterium/clasificación , Methylobacterium/metabolismo , Methylophilaceae/clasificación , Methylophilaceae/metabolismo , Metiltransferasas/genética , Metiltransferasas/metabolismo , Microbiología del Suelo
4.
J Environ Qual ; 47(2): 254-262, 2018 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-29634809

RESUMEN

Chloromethane (CHCl, methyl chloride) is the most abundant volatile halocarbon in the atmosphere and involved in stratospheric ozone depletion. The global CHCl budget, and especially the CHCl sink from microbial degradation in soil, still involves large uncertainties. These may potentially be resolved by a combination of stable isotope analysis and bacterial diversity studies. We determined the stable isotope fractionation of CHCl hydrogen and carbon and investigated bacterial diversity during CHCl degradation in three soils with different properties (forest, grassland, and agricultural soils) and at different temperatures and headspace mixing ratios of CHCl. The extent of chloromethane degradation decreased in the order forest > grassland > agricultural soil. Rates ranged from 0.7 to 2.5 µg g dry wt. d for forest soil, from 0.1 to 0.9 µg g dry wt. d for grassland soil, and from 0.1 to 0.4 µg g dry wt. d for agricultural soil and increased with increasing temperature and CHCl supplementation. The measured mean stable hydrogen enrichment factor of CHCl of -50 ± 13‰ was unaffected by temperature, mixing ratio, or soil type. In contrast, the stable carbon enrichment factor depended on CHCl degradation rates and ranged from -38 to -11‰. Bacterial community composition correlated with soil properties was independent from CHCl degradation or isotope enrichment. Nevertheless, increased abundance after CHCl incubation was observed in 21 bacterial operational taxonomical units (OTUs at the 97% 16S RNA sequence identity level). This suggests that some of these bacterial taxa, although not previously associated with CHCl degradation, may play a role in the microbial CHCl sink in soil.


Asunto(s)
Cloruro de Metilo/química , Microbiología del Suelo , Agricultura , Isótopos , Suelo
5.
J Bacteriol ; 196(11): 2101-7, 2014 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-24682326

RESUMEN

Horizontal gene transfer plays a crucial role in microbial evolution. While much is known about the mechanisms that determine whether physical DNA can be transferred into a new host, the factors determining the utility of the transferred genes are less clear. We have explored this issue using dichloromethane consumption in Methylobacterium strains. Methylobacterium extorquens DM4 expresses a dichloromethane dehalogenase (DcmA) that has been acquired through horizontal gene transfer and allows the strain to grow on dichloromethane as the sole carbon and energy source. We transferred the dcmA gene into six Methylobacterium strains that include both close and distant evolutionary relatives. The transconjugants varied in their ability to grow on dichloromethane, but their fitness on dichloromethane did not correlate with the phylogeny of the parental strains or with any single tested physiological factor. This work highlights an important limiting factor in horizontal gene transfer, namely, the capacity of the recipient strain to accommodate the stress and metabolic disruption resulting from the acquisition of a new enzyme or pathway. Understanding these limitations may help to rationalize historical examples of horizontal transfer and aid deliberate genetic transfers in biotechnology for metabolic engineering.


Asunto(s)
Regulación Bacteriana de la Expresión Génica/fisiología , Transferencia de Gen Horizontal/fisiología , Liasas/metabolismo , Methylobacterium/clasificación , Methylobacterium/genética , Filogenia , Regulación Enzimológica de la Expresión Génica/fisiología , Concentración de Iones de Hidrógeno , Liasas/genética , Cloruro de Metileno/metabolismo
6.
Appl Environ Microbiol ; 80(11): 3541-50, 2014 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-24682302

RESUMEN

Monomethylamine (MMA, CH3NH2) can be used as a carbon and nitrogen source by many methylotrophic bacteria. Methylobacterium extorquens DM4 lacks the MMA dehydrogenase encoded by mau genes, which in M. extorquens AM1 is essential for growth on MMA. Identification and characterization of minitransposon mutants with an MMA-dependent phenotype showed that strain DM4 grows with MMA as the sole source of carbon, energy, and nitrogen by the N-methylglutamate (NMG) pathway. Independent mutations were found in a chromosomal region containing the genes gmaS, mgsABC, and mgdABCD for the three enzymes of the pathway, γ-glutamylmethylamide (GMA) synthetase, NMG synthase, and NMG dehydrogenase, respectively. Reverse transcription-PCR confirmed the operonic structure of the two divergent gene clusters mgsABC-gmaS and mgdABCD and their induction during growth with MMA. The genes mgdABCD and mgsABC were found to be essential for utilization of MMA as a carbon and nitrogen source. The gene gmaS was essential for MMA utilization as a carbon source, but residual growth of mutant DM4gmaS growing with succinate and MMA as a nitrogen source was observed. Plasmid copies of gmaS and the gmaS homolog METDI4690, which encodes a protein 39% identical to GMA synthetase, fully restored the ability of mutants DM4gmaS and DM4gmaSΔmetdi4690 to use MMA as a carbon and nitrogen source. Similarly, chemically synthesized GMA, the product of GMA synthetase, could be used as a nitrogen source for growth in the wild-type strain, as well as in DM4gmaS and DM4gmaSΔmetdi4690 mutants. The NADH:ubiquinone oxidoreductase respiratory complex component NuoG was also found to be essential for growth with MMA as a carbon source.


Asunto(s)
Glutamatos/metabolismo , Redes y Vías Metabólicas/genética , Metilaminas/metabolismo , Methylobacterium extorquens/genética , Methylobacterium extorquens/metabolismo , Carbono/metabolismo , Elementos Transponibles de ADN , Metabolismo Energético , Eliminación de Gen , Perfilación de la Expresión Génica , Prueba de Complementación Genética , Methylobacterium extorquens/crecimiento & desarrollo , Familia de Multigenes , Mutagénesis Insercional , Nitrógeno/metabolismo , Transcripción Genética
7.
Microb Cell Fact ; 13(1): 97, 2014 Jul 05.
Artículo en Inglés | MEDLINE | ID: mdl-24997803

RESUMEN

BACKGROUND: The use of fossil carbon sources for fuels and petrochemicals has serious impacts on our environment and is unable to meet the demand in the future. A promising and sustainable alternative is to substitute fossil carbon sources with microbial cell factories converting lignocellulosic biomass into desirable value added products. However, such bioprocesses require availability of suitable and efficient microbial biocatalysts, capable of utilizing C5 sugars and tolerant to inhibitory compounds generated during pretreatment of biomass. In this study, the performance of a collection of lactic acid bacteria was evaluated regarding their properties with respect to the conversion of lignocellulosic feedstocks. The strains were examined for their ability to utilize xylose and arabinose as well as their resistance towards common inhibitors from pretreated lignocellulosic biomass (furan derivatives, phenolic compounds, weak acids). RESULTS: Among 296 tested Lactobacillus and Pediococcus strains, 3 L. pentosus, 1 P. acidilactici and 1 P. pentosaceus isolates were found to be both capable of utilizing xylose and arabinose and highly resistant to the key inhibitors from chemically pretreated lignocellulosic biomass. When tested in broth with commonly found combinations of inhibitors, the selected strains showed merely 4%, 1% and 37% drop in growth rates for sugarcane bagasse, wheat straw and soft wood representatives, respectively, as compared to Escherichia coli MG1655 showing decreased growth rates by 36%, 21% and 90%, respectively, under the same conditions. CONCLUSION: The study showed that some strains of Lactobacilli and Pediococci have the potential to be used as production platforms for value-added products from pretreated lignocellulosic biomass. Selected Lactobacilli and Pediococci strains were able to tolerate the key inhibitors in higher concentrations compared to E.coli; in addition, as these isolates were also capable of fermenting xylose and arabinose, they constitute good candidates for efficient lignocellulosic feedstock bioconversions.


Asunto(s)
Etanol/metabolismo , Lactobacillaceae/metabolismo , Lignina/metabolismo , Arabinosa/metabolismo , Biocombustibles/análisis , Microbiología Ambiental , Fermentación , Microbiología de Alimentos , Ácido Láctico/metabolismo , Lactobacillaceae/clasificación , Lactobacillaceae/genética , Lactobacillaceae/aislamiento & purificación , Xilosa/metabolismo
8.
Appl Environ Microbiol ; 79(21): 6561-7, 2013 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-23956392

RESUMEN

Methyl halides are volatile one-carbon compounds responsible for substantial depletion of stratospheric ozone. Among them, chloromethane (CH3Cl) is the most abundant halogenated hydrocarbon in the atmosphere. Global budgets of methyl halides in the environment are still poorly understood due to uncertainties in their natural sources, mainly from vegetation, and their sinks, which include chloromethane-degrading bacteria. A bacterial bioreporter for the detection of methyl halides was developed on the basis of detailed knowledge of the physiology and genetics of Methylobacterium extorquens CM4, an aerobic alphaproteobacterium which utilizes chloromethane as the sole source of carbon and energy. A plasmid construct with the promoter region of the chloromethane dehalogenase gene cmuA fused to a promotorless yellow fluorescent protein gene cassette resulted in specific methyl halide-dependent fluorescence when introduced into M. extorquens CM4. The bacterial whole-cell bioreporter allowed detection of methyl halides at femtomolar levels and quantification at concentrations above 10 pM (approximately 240 ppt). As shown for the model chloromethane-producing plant Arabidopsis thaliana in particular, the bioreporter may provide an attractive alternative to analytical chemical methods to screen for natural sources of methyl halide emissions.


Asunto(s)
Monitoreo del Ambiente/métodos , Contaminantes Ambientales/análisis , Cloruro de Metilo/análisis , Methylobacterium extorquens/enzimología , Metiltransferasas/metabolismo , Arabidopsis/metabolismo , Proteínas Bacterianas/genética , Cartilla de ADN/genética , Fluorometría , Proteínas Luminiscentes/genética , Cloruro de Metilo/metabolismo , Metiltransferasas/genética , Microscopía Confocal , Microscopía Fluorescente , Plásmidos/genética , Regiones Promotoras Genéticas/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
9.
J Bacteriol ; 194(2): 551-2, 2012 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-22207753

RESUMEN

Methylomicrobium strains are widespread in saline environments. Here, we report the complete genome sequence of Methylomicrobium alcaliphilum 20Z, a haloalkaliphilic methanotrophic bacterium, which will provide the basis for detailed characterization of the core pathways of both single-carbon metabolism and responses to osmotic and high-pH stresses. Final assembly of the genome sequence revealed that this bacterium contains a 128-kb plasmid, making M. alcaliphilum 20Z the first methanotrophic bacterium of known genome sequence for which a plasmid has been reported.


Asunto(s)
Gammaproteobacteria/genética , Genoma Bacteriano , Datos de Secuencia Molecular , Plásmidos/genética
11.
J Proteome Res ; 11(1): 109-18, 2012 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-22040141

RESUMEN

The identification of cell determinants involved in probiotic features is a challenge in current probiotic research. In this work, markers of bile tolerance in Lactobacillus casei were investigated using comparative proteomics. Six L. casei strains were classified on the basis of their ability to grow in the presence of bile salts in vitro. Constitutive differences between whole cell proteomes of the most tolerant strain (L. casei Rosell-215), the most sensitive one (L. casei ATCC 334), and a moderately tolerant strain (L. casei DN-114 001) were investigated. The ascertained subproteome was further studied for the six strains in both standard and bile stressing conditions. Focus was on proteins whose expression levels were correlated with observed levels of bile tolerance in vitro, particularly those previously reported to be involved in the bile tolerance process of lactobacilli. Analysis revealed that 12 proteins involved in membrane modification (NagA, NagB, and RmlC), cell protection and detoxification (ClpL and OpuA), as well as central metabolism (Eno, GndA, Pgm, Pta, Pyk, Rp1l, and ThRS) were likely to be key determinants of bile tolerance in L. casei and may serve as potential biomarkers for phenotyping or screening purposes. The approach used enabled the correlation of expression levels of particular proteins with a specific probiotic trait.


Asunto(s)
Proteínas Bacterianas/metabolismo , Ácidos y Sales Biliares/farmacología , Lacticaseibacillus casei/fisiología , Proteoma/metabolismo , Estrés Fisiológico , Animales , Proteínas Bacterianas/genética , Biomarcadores/metabolismo , Bovinos , Análisis por Conglomerados , Expresión Génica , Lacticaseibacillus casei/efectos de los fármacos , Lacticaseibacillus casei/crecimiento & desarrollo , Probióticos , Proteoma/genética , Proteómica , Estadísticas no Paramétricas
12.
Environ Microbiome ; 17(1): 24, 2022 May 08.
Artículo en Inglés | MEDLINE | ID: mdl-35527282

RESUMEN

BACKGROUND: Chloromethane (CH3Cl) is the most abundant halogenated organic compound in the atmosphere and substantially responsible for the destruction of the stratospheric ozone layer. Since anthropogenic CH3Cl sources have become negligible with the application of the Montreal Protocol (1987), natural sources, such as vegetation and soils, have increased proportionally in the global budget. CH3Cl-degrading methylotrophs occurring in soils might be an important and overlooked sink. RESULTS AND CONCLUSIONS: The objective of our study was to link the biotic CH3Cl sink with the identity of active microorganisms and their biochemical pathways for CH3Cl degradation in a deciduous forest soil. When tested in laboratory microcosms, biological CH3Cl consumption occurred in leaf litter, senescent leaves, and organic and mineral soil horizons. Highest consumption rates, around 2 mmol CH3Cl g-1 dry weight h-1, were measured in organic soil and senescent leaves, suggesting that top soil layers are active (micro-)biological CH3Cl degradation compartments of forest ecosystems. The DNA of these [13C]-CH3Cl-degrading microbial communities was labelled using stable isotope probing (SIP), and the corresponding taxa and their metabolic pathways studied using high-throughput metagenomics sequencing analysis. [13C]-labelled Metagenome-Assembled Genome closely related to the family Beijerinckiaceae may represent a new methylotroph family of Alphaproteobacteria, which is found in metagenome databases of forest soils samples worldwide. Gene markers of the only known pathway for aerobic CH3Cl degradation, via the methyltransferase system encoded by the CH3Cl utilisation genes (cmu), were undetected in the DNA-SIP metagenome data, suggesting that biological CH3Cl sink in this deciduous forest soil operates by a cmu-independent metabolism.

13.
Microorganisms ; 10(7)2022 Jun 27.
Artículo en Inglés | MEDLINE | ID: mdl-35889020

RESUMEN

Dichloromethane (DCM, methylene chloride) is a toxic halogenated volatile organic compound massively used for industrial applications, and consequently often detected in the environment as a major pollutant. DCM biotransformation suggests a sustainable decontamination strategy of polluted sites. Among methylotrophic bacteria able to use DCM as a sole source of carbon and energy for growth, Methylorubrum extorquens DM4 is a longstanding reference strain. Here, the primary 5'-ends of transcripts were obtained using a differential RNA-seq (dRNA-seq) approach to provide the first transcription start site (TSS) genome-wide landscape of a methylotroph using DCM or methanol. In total, 7231 putative TSSs were annotated and classified with respect to their localization to coding sequences (CDSs). TSSs on the opposite strand of CDS (antisense TSS) account for 31% of all identified TSSs. One-third of the detected TSSs were located at a distance to the start codon inferior to 250 nt (average of 84 nt) with 7% of leaderless mRNA. Taken together, the global TSS map for bacterial growth using DCM or methanol will facilitate future studies in which transcriptional regulation is crucial, and efficient DCM removal at polluted sites is limited by regulatory processes.

14.
Microbiol Resour Announc ; 11(10): e0068422, 2022 Oct 20.
Artículo en Inglés | MEDLINE | ID: mdl-36106890

RESUMEN

The complete genome of Sphingomonas aerolata PDD-32b-11, a bacterium isolated from cloud water, was sequenced. It features four circular replicons, a chromosome of 3.99 Mbp, and three plasmids. Two putative rhodopsin-encoding genes were detected which might act as proton pumps to harvest light energy.

15.
G3 (Bethesda) ; 12(12)2022 12 01.
Artículo en Inglés | MEDLINE | ID: mdl-36259934

RESUMEN

The genome of the basidiomycete yeast Dioszegia hungarica strain PDD-24b-2 isolated from cloud water at the summit of puy de Dôme (France) was sequenced using a hybrid PacBio and Illumina sequencing strategy. The obtained assembled genome of 20.98 Mb and a GC content of 57% is structured in 16 large-scale contigs ranging from 90 kb to 5.56 Mb, and another 27.2 kb contig representing the complete circular mitochondrial genome. In total, 8,234 proteins were predicted from the genome sequence. The mitochondrial genome shows 16.2% cgu codon usage for arginine but has no canonical cognate tRNA to translate this codon. Detected transposable element (TE)-related sequences account for about 0.63% of the assembled genome. A dataset of 2,068 hand-picked public environmental metagenomes, representing over 20 Tbp of raw reads, was probed for D. hungarica related ITS sequences, and revealed worldwide distribution of this species, particularly in aerial habitats. Growth experiments suggested a psychrophilic phenotype and the ability to disperse by producing ballistospores. The high-quality assembled genome obtained for this D. hungarica strain will help investigate the behavior and ecological functions of this species in the environment.


Asunto(s)
Basidiomycota , Saccharomyces cerevisiae , Saccharomyces cerevisiae/genética , Agua , Basidiomycota/genética , Análisis de Secuencia de ADN
16.
J Bacteriol ; 193(17): 4541-2, 2011 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-21725020

RESUMEN

Rhodocyclales are representative of versatile bacteria that are able to utilize a wide variety of organic compounds for growth, but only a few strains have been isolated in pure culture thus far. Here we present the genome sequence of Methyloversatilis universalis FAM5(T), the first cultivable methylotrophic member of the order.


Asunto(s)
Genoma Bacteriano , Rhodocyclaceae/genética , Rhodocyclaceae/aislamiento & purificación , ADN Bacteriano/genética , ADN Bacteriano/aislamiento & purificación , Sedimentos Geológicos/microbiología , Datos de Secuencia Molecular , Familia de Multigenes , Oxidación-Reducción , Filogenia , Análisis de Secuencia de ADN , Transducción de Señal
17.
J Bacteriol ; 193(18): 5035-6, 2011 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-21868803

RESUMEN

Hyphomicrobium sp. strain MC1 is an aerobic methylotroph originally isolated from industrial sewage. This prosthecate bacterium was the first strain reported to grow with chloromethane as the sole carbon and energy source. Its genome, consisting of a single 4.76-Mb chromosome, is the first for a chloromethane-degrading bacterium to be formally reported.


Asunto(s)
ADN Bacteriano/química , ADN Bacteriano/genética , Genoma Bacteriano , Hyphomicrobium/genética , Análisis de Secuencia de ADN , Aerobiosis , Carbono/metabolismo , Hyphomicrobium/aislamiento & purificación , Hyphomicrobium/metabolismo , Hyphomicrobium/fisiología , Residuos Industriales , Cloruro de Metilo/metabolismo , Datos de Secuencia Molecular , Aguas del Alcantarillado/microbiología
18.
J Bacteriol ; 193(22): 6418-9, 2011 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-21725021

RESUMEN

Methylobacter tundripaludum SV96(T) (ATCC BAA-1195) is a psychrotolerant aerobic methane-oxidizing gammaproteobacterium (Methylococcales, Methylococcaceae) living in High Arctic wetland soil. The strain was isolated from soil harvested in July 1996 close to the settlement Ny-Ålesund, Svalbard, Norway (78°56'N, 11°53'E), and described as a novel species in 2006. The genome includes pmo and pxm operons encoding copper membrane monooxygenases (Cu-MMOs), genes required for nitrogen fixation, and the nirS gene implicated in dissimilatory nitrite reduction to NO but no identifiable inventory for further processing of nitrogen oxides. These genome data provide the basis to investigate M. tundripaludum SV96, identified as a major player in the biogeochemistry of Arctic environments.


Asunto(s)
Genoma Bacteriano , Metano/metabolismo , Methylococcaceae/genética , Regiones Árticas , Secuencia de Bases , Methylococcaceae/aislamiento & purificación , Methylococcaceae/metabolismo , Datos de Secuencia Molecular , Microbiología del Suelo
19.
J Bacteriol ; 193(10): 2668-9, 2011 May.
Artículo en Inglés | MEDLINE | ID: mdl-21441518

RESUMEN

Methylocystis sp. strain Rockwell (ATCC 49242) is an aerobic methane-oxidizing alphaproteobacterium isolated from an aquifer in southern California. Unlike most methanotrophs in the Methylocystaceae family, this strain has a single pmo operon encoding particulate methane monooxygenase but no evidence of the genes encoding soluble methane monooxygenase. This is the first reported genome sequence of a member of the Methylocystis species of the Methylocystaceae family in the order Rhizobiales.


Asunto(s)
ADN Bacteriano/química , ADN Bacteriano/genética , Genoma Bacteriano , Methylocystaceae/genética , Aerobiosis , California , Metano/metabolismo , Methylocystaceae/aislamiento & purificación , Methylocystaceae/fisiología , Datos de Secuencia Molecular , Operón , Oxigenasas/genética , Análisis de Secuencia de ADN , Microbiología del Agua
20.
Environ Microbiol ; 13(9): 2518-35, 2011 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-21854516

RESUMEN

Dichloromethane (CH(2)Cl(2) , DCM) is a chlorinated solvent mainly produced by industry, and a common pollutant. Some aerobic methylotrophic bacteria are able to grow with this chlorinated methane as their sole carbon and energy source, using a DCM dehalogenase/glutathione S-transferase encoded by dcmA to transform DCM into two molecules of HCl and one molecule of formaldehyde, a toxic intermediate of methylotrophic metabolism. In Methylobacterium extorquens DM4 of known genome sequence, dcmA lies on a 126 kb dcm genomic island not found so far in other DCM-dechlorinating strains. An experimental search for the molecular determinants involved in specific cellular responses of strain DM4 growing with DCM was performed. Random mutagenesis with a minitransposon containing a promoterless reporter gfp gene yielded 25 dcm mutants with a specific DCM-associated phenotype. Differential proteomic analysis of cultures grown with DCM and with methanol defined 38 differentially abundant proteins. The 5.5 kb dcm islet directly involved in DCM dehalogenation is the only one of seven gene clusters specific to the DCM response to be localized within the dcm genomic island. The DCM response was shown to involve mainly the core genome of Methylobacterium extorquens, providing new insights on DCM-dependent adjustments of C1 metabolism and gene regulation, and suggesting a specific stress response of Methylobacterium during growth with DCM. Fatty acid, hopanoid and peptidoglycan metabolisms were affected, hinting at the membrane-active effects of DCM due to its solvent properties. A chloride-induced efflux transporter termed CliABC was also newly identified. Thus, DCM dechlorination driven by the dcm islet elicits a complex adaptive response encoded by the core genome common to dechlorinating as well as non-dechlorinating Methylobacterium strains.


Asunto(s)
Genoma Bacteriano , Cloruro de Metileno/metabolismo , Methylobacterium extorquens/genética , Adaptación Fisiológica , Biodegradación Ambiental , Genómica/métodos , Glutatión Transferasa/genética , Glutatión Transferasa/metabolismo , Liasas/genética , Liasas/metabolismo , Metanol/metabolismo , Methylobacterium extorquens/crecimiento & desarrollo , Methylobacterium extorquens/metabolismo , Familia de Multigenes , Mutagénesis Insercional , Fenotipo , Proteómica
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA