RESUMEN
Wnt-ß-catenin signaling regulates cell fate during organ development and postnatal tissue maintenance, but its contribution to specification of distinct lung epithelial lineages is still unclear. To address this question, we used a Cre recombinase (Cre)-LoxP approach to activate canonical Wnt signaling ectopically in developing lung endoderm. We found that persistent activation of canonical Wnt signaling within distal lung endoderm was permissive for normal development of alveolar epithelium, yet led to the loss of developing bronchiolar epithelium and ectasis of distal conducting airways. Activation of canonical Wnt led to ectopic expression of a lymphoid-enhancing factor and a T-cell factor (LEF and TCF, respectively) and absence of SRY (sex-determining region Y)-box 2 (SOX2) and tumor protein p63 (p63) expression in proximal derivatives. Conditional loss of SOX2 in airways phenocopied epithelial differentiation defects observed with ectopic activation of canonical Wnt. Our data suggest that Wnt negatively regulates a SOX2-dependent signaling program required for developmental progression of the bronchiolar lineage.
Asunto(s)
Células Epiteliales/citología , Células Epiteliales/metabolismo , Pulmón/citología , Factores de Transcripción SOXB1/metabolismo , Vía de Señalización Wnt , beta Catenina/metabolismo , Animales , Apoptosis , Bronquiolos/citología , Bronquiolos/metabolismo , Diferenciación Celular , Linaje de la Célula , Proliferación Celular , Endodermo/metabolismo , Femenino , Regulación de la Expresión Génica , Genes Reporteros , Pulmón/metabolismo , Factor de Unión 1 al Potenciador Linfoide/metabolismo , Masculino , Ratones , Ratones Transgénicos , Fosfoproteínas/metabolismo , Estabilidad Proteica , Alveolos Pulmonares/citología , Alveolos Pulmonares/metabolismo , Factor de Transcripción SOX9/metabolismo , Factores de Transcripción SOXB1/deficiencia , Factores de Transcripción TCF/metabolismo , Transactivadores/metabolismo , Transcripción Genética , Proteínas Wnt/metabolismo , beta Catenina/biosíntesis , beta Catenina/genéticaRESUMEN
Mechanisms that regulate regional epithelial cell diversity and pathologic remodeling in airways are poorly understood. We hypothesized that regional differences in cell composition and injury-related tissue remodeling result from the type and composition of local progenitors. We used surface markers and the spatial expression pattern of an SFTPC-GFP transgene to subset epithelial progenitors by airway region. Green fluorescent protein (GFP) expression ranged from undetectable to high in a proximal-to-distal gradient. GFP(hi) cells were subdivided by CD24 staining into alveolar (CD24(neg)) and conducting airway (CD24(low)) populations. This allowed for the segregation of three types of progenitors displaying distinct clonal behavior in vitro. GFP(neg) and GFP(low) progenitors both yielded lumen containing colonies but displayed transcriptomes reflective of pseudostratified and distal conducting airways, respectively. CD24(low)GFP(hi) progenitors were present in an overlapping distribution with GFP(low) progenitors in distal airways, yet expressed lower levels of Sox2 and expanded in culture to yield undifferentiated self-renewing progeny. Colony-forming ability was reduced for each progenitor cell type after in vivo bleomycin exposure, but only CD24(low) GFP(hi) progenitors showed robust expansion during tissue remodeling. These data reveal intrinsic differences in the properties of regional progenitors and suggest that their unique responses to tissue damage drive local tissue remodeling.
Asunto(s)
Lesión Pulmonar/patología , Células Madre/efectos de los fármacos , Células Madre/patología , Animales , Bleomicina , Células Epiteliales/efectos de los fármacos , Células Epiteliales/metabolismo , Células Epiteliales/patología , Lesión Pulmonar/inducido químicamente , Lesión Pulmonar/metabolismo , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C3H , Ratones Endogámicos C57BL , Análisis por Micromatrices , Sistema Respiratorio/efectos de los fármacos , Sistema Respiratorio/patología , Células Madre/metabolismo , Uteroglobina/biosíntesisRESUMEN
Signaling by Wnt/beta-catenin regulates self-renewal of tissue stem cells in the gut and, when activated in the embryonic bronchiolar epithelium, leads to stem cell expansion. We have used transgenic and cell type-specific knockout strategies to determine roles for beta-catenin-regulated gene expression in normal maintenance and repair of the bronchiolar epithelium. Analysis of TOPGal transgene activity detected beta-catenin signaling in the steady-state and repairing bronchiolar epithelium. However, the broad distribution and phenotype of signaling cells precluded establishment of a clear role for beta-catenin in the normal or repairing state. Necessity of beta-catenin signaling was tested through Cre-mediated deletion of Catnb exons 2-6 in airway epithelial cells. Functional knockout of beta-catenin had no impact on expression of Clara cell differentiation markers, mitotic index, or sensitivity of these cells to the Clara cell-specific toxicant, naphthalene. Repair of the naphthalene-injured airway proceeded with establishment of focal regions of beta-catenin-null epithelium. The size of regenerative epithelial units, mitotic index, and restoration of the ciliated cell population did not vary between wild-type and genetically modified mice. Thus, beta-catenin was not necessary for maintenance or efficient repair of the bronchiolar epithelium.
Asunto(s)
Bronquiolos/efectos de los fármacos , Regeneración , Mucosa Respiratoria/efectos de los fármacos , Transducción de Señal , Células Madre/efectos de los fármacos , beta Catenina/metabolismo , Animales , Bronquiolos/metabolismo , Bronquiolos/patología , Diferenciación Celular , Proliferación Celular , Regulación del Desarrollo de la Expresión Génica , Técnicas de Inactivación de Genes , Integrasas/genética , Operón Lac , Ratones , Ratones Endogámicos C57BL , Ratones Transgénicos , Índice Mitótico , Naftalenos/toxicidad , Fenotipo , Regiones Promotoras Genéticas , ARN Mensajero/metabolismo , Ratas , Regeneración/efectos de los fármacos , Regeneración/genética , Mucosa Respiratoria/metabolismo , Mucosa Respiratoria/patología , Transducción de Señal/efectos de los fármacos , Transducción de Señal/genética , Células Madre/metabolismo , Células Madre/patología , Factores de Transcripción TCF/genética , Factores de Tiempo , Proteína 2 Similar al Factor de Transcripción 7 , Uteroglobina/genética , beta Catenina/genéticaRESUMEN
Bronchiolar Clara cells undergo phenotypic changes during development and in disease. These changes are poorly described due to a paucity of molecular markers. We used chemical and transgenic approaches to ablate Clara cells, allowing identification of their unique gene expression profile. Flavin monooxygenase 3 (Fmo3), paraoxonase 1 (Pon1), aldehyde oxidase 3 (Aox3), and claudin 10 (Cldn10) were identified as novel Clara cell markers. New and existing Clara cell marker genes were categorized into three classes based on their unique developmental expression pattern. Cldn10 was uniformly expressed in the epithelium at Embryonic Day (E)14.5 and became restricted to secretory cells at E18.5. This transition was defined by induction of CCSP. Maturation of secretory cells was associated with progressive increases in the expression of Fmo3, Pon1, Aox3, and Cyp2f2 between late embryonic and postnatal periods. Messenger RNA abundance of all categories of genes was dramatically decreased after naphthalene-induced airway injury, and displayed a sequence of temporal induction during repair that suggested sequential secretory cell maturation. We have defined a broader repertoire of Clara cell-specific genes that allows staging of epithelial maturation during development and repair.
Asunto(s)
Biomarcadores/metabolismo , Células Epiteliales/fisiología , Epitelio/fisiología , Mucosa Respiratoria/citología , Animales , Diferenciación Celular , Claudinas , Células Epiteliales/citología , Perfilación de la Expresión Génica , Pulmón/anatomía & histología , Pulmón/efectos de los fármacos , Pulmón/embriología , Pulmón/fisiología , Masculino , Proteínas de la Membrana/genética , Proteínas de la Membrana/metabolismo , Ratones , Ratones Transgénicos , Análisis por Micromatrices , Datos de Secuencia Molecular , Naftalenos/farmacología , Mucosa Respiratoria/efectos de los fármacos , Mucosa Respiratoria/fisiologíaRESUMEN
Maintenance of classic stem cell hierarchies is dependent upon stem cell self-renewal mediated in part by Wnt/beta-catenin regulation of the cell cycle. This function is critical in rapidly renewing tissues due to the obligate role played by the tissue stem cell. However, the stem cell hierarchy responsible for maintenance of the conducting airway epithelium is distinct from classic stem cell hierarchies. The epithelium of conducting airways is maintained by transit-amplifying cells in the steady state; rare bronchiolar stem cells are activated to participate in epithelial repair only following depletion of transit-amplifying cells. Here, we investigate how signaling through beta-catenin affects establishment and maintenance of the stem cell hierarchy within the slowly renewing epithelium of the lung. Conditional potentiation of beta-catenin signaling in the embryonic lung results in amplification of airway stem cells through attenuated differentiation rather than augmented proliferation. Our data demonstrate that the differentiation-modulating activities of stabilized beta-catenin account for expansion of tissue stem cells.