RESUMEN
The quality of the adaptive immune response depends on the differentiation of distinct CD4(+) helper T cell subsets, and the magnitude of an immune response is controlled by CD4(+)Foxp3(+) regulatory T cells (Treg cells). However, how a tissue- and cell type-specific suppressor program of Treg cells is mechanistically orchestrated has remained largely unexplored. Through the use of Treg cell-specific gene targeting, we found that the suppression of allergic immune responses in the lungs mediated by T helper type 2 (TH2) cells was dependent on the activity of the protein kinase CK2. Genetic ablation of the ß-subunit of CK2 specifically in Treg cells resulted in the proliferation of a hitherto-unexplored ILT3(+) Treg cell subpopulation that was unable to control the maturation of IRF4(+)PD-L2(+) dendritic cells required for the development of TH2 responses in vivo.
Asunto(s)
Quinasa de la Caseína II/inmunología , Linfocitos T Reguladores/inmunología , Células Th2/inmunología , Animales , Linfocitos T CD4-Positivos/enzimología , Linfocitos T CD4-Positivos/inmunología , Diferenciación Celular/inmunología , Procesos de Crecimiento Celular/inmunología , Línea Celular , Células Dendríticas/enzimología , Células Dendríticas/inmunología , Factores de Transcripción Forkhead/inmunología , Humanos , Hipersensibilidad/sangre , Hipersensibilidad/inmunología , Factores Reguladores del Interferón/inmunología , Leucocitos Mononucleares/inmunología , Masculino , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Ratones Transgénicos , Receptores de Superficie Celular/inmunología , Linfocitos T Reguladores/enzimología , Células Th2/enzimologíaRESUMEN
We report the identification of 67 previously undescribed histone modifications, increasing the current number of known histone marks by about 70%. We further investigated one of the marks, lysine crotonylation (Kcr), confirming that it represents an evolutionarily-conserved histone posttranslational modification. The unique structure and genomic localization of histone Kcr suggest that it is mechanistically and functionally different from histone lysine acetylation (Kac). Specifically, in both human somatic and mouse male germ cell genomes, histone Kcr marks either active promoters or potential enhancers. In male germinal cells immediately following meiosis, Kcr is enriched on sex chromosomes and specifically marks testis-specific genes, including a significant proportion of X-linked genes that escape sex chromosome inactivation in haploid cells. These results therefore dramatically extend the repertoire of histone PTM sites and designate Kcr as a specific mark of active sex chromosome-linked genes in postmeiotic male germ cells.
Asunto(s)
Regulación de la Expresión Génica , Código de Histonas , Animales , Células HeLa , Histonas/química , Histonas/metabolismo , Humanos , Lisina/metabolismo , Masculino , Meiosis , Ratones , Procesamiento Proteico-Postraduccional , Testículo/citología , Testículo/metabolismoRESUMEN
Histone replacement by transition proteins (TPs) and protamines (Prms) constitutes an essential step for the successful production of functional male gametes, yet nothing is known on the underlying functional interplay between histones, TPs, and Prms. Here, by studying spermatogenesis in the absence of a spermatid-specific histone variant, H2A.L.2, we discover a fundamental mechanism involved in the transformation of nucleosomes into nucleoprotamines. H2A.L.2 is synthesized at the same time as TPs and enables their loading onto the nucleosomes. TPs do not displace histones but rather drive the recruitment and processing of Prms, which are themselves responsible for histone eviction. Altogether, the incorporation of H2A.L.2 initiates and orchestrates a series of successive transitional states that ultimately shift to the fully compacted genome of the mature spermatozoa. Hence, the current view of histone-to-nucleoprotamine transition should be revisited and include an additional step with H2A.L.2 assembly prior to the action of TPs and Prms.
Asunto(s)
Ensamble y Desensamble de Cromatina , Cromatina/metabolismo , Histonas/metabolismo , Nucleosomas/metabolismo , Protaminas/metabolismo , Espermatogénesis , Espermatozoides/metabolismo , Animales , Células COS , Chlorocebus aethiops , Cromatina/genética , Proteínas Cromosómicas no Histona/genética , Proteínas Cromosómicas no Histona/metabolismo , Biología Computacional , Bases de Datos Genéticas , Fertilidad , Regulación del Desarrollo de la Expresión Génica , Predisposición Genética a la Enfermedad , Genoma , Histonas/deficiencia , Histonas/genética , Infertilidad Masculina/genética , Infertilidad Masculina/metabolismo , Infertilidad Masculina/patología , Infertilidad Masculina/fisiopatología , Masculino , Ratones de la Cepa 129 , Ratones Noqueados , Nucleosomas/genética , Fenotipo , Espermatogénesis/genética , Espermatozoides/patología , TransfecciónRESUMEN
Recently discovered histone lysine acylation marks increase the functional diversity of nucleosomes well beyond acetylation. Here, we focus on histone butyrylation in the context of sperm cell differentiation. Specifically, we investigate the butyrylation of histone H4 lysine 5 and 8 at gene promoters where acetylation guides the binding of Brdt, a bromodomain-containing protein, thereby mediating stage-specific gene expression programs and post-meiotic chromatin reorganization. Genome-wide mapping data show that highly active Brdt-bound gene promoters systematically harbor competing histone acetylation and butyrylation marks at H4 K5 and H4 K8. Despite acting as a direct stimulator of transcription, histone butyrylation competes with acetylation, especially at H4 K5, to prevent Brdt binding. Additionally, H4 K5K8 butyrylation also marks retarded histone removal during late spermatogenesis. Hence, alternating H4 acetylation and butyrylation, while sustaining direct gene activation and dynamic bromodomain binding, could impact the final male epigenome features.
Asunto(s)
Butiratos/metabolismo , Epigénesis Genética , Regulación del Desarrollo de la Expresión Génica , Histonas/metabolismo , Proteínas Nucleares/genética , Regiones Promotoras Genéticas , Procesamiento Proteico-Postraduccional , Espermatocitos/metabolismo , Acetilación , Animales , Sitios de Unión , Diferenciación Celular , Ensamble y Desensamble de Cromatina , Estudio de Asociación del Genoma Completo , Histonas/química , Histonas/genética , Lisina , Masculino , Ratones , Proteínas Nucleares/química , Proteínas Nucleares/metabolismo , Conformación Proteica , Relación Estructura-Actividad , Transcripción Genética , Activación TranscripcionalRESUMEN
The histone variant H3.3 is encoded by two distinct genes, H3f3a and H3f3b, exhibiting identical amino-acid sequence. H3.3 is required for spermatogenesis, but the molecular mechanism of its spermatogenic function remains obscure. Here, we have studied the role of each one of H3.3A and H3.3B proteins in spermatogenesis. We have generated transgenic conditional knock-out/knock-in (cKO/KI) epitope-tagged FLAG-FLAG-HA-H3.3B (H3.3BHA) and FLAG-FLAG-HA-H3.3A (H3.3AHA) mouse lines. We show that H3.3B, but not H3.3A, is required for spermatogenesis and male fertility. Analysis of the molecular mechanism unveils that the absence of H3.3B led to alterations in the meiotic/post-meiotic transition. Genome-wide RNA-seq reveals that the depletion of H3.3B in meiotic cells is associated with increased expression of the whole sex X and Y chromosomes as well as of both RLTR10B and RLTR10B2 retrotransposons. In contrast, the absence of H3.3B resulted in down-regulation of the expression of piRNA clusters. ChIP-seq experiments uncover that RLTR10B and RLTR10B2 retrotransposons, the whole sex chromosomes and the piRNA clusters are markedly enriched of H3.3. Taken together, our data dissect the molecular mechanism of H3.3B functions during spermatogenesis and demonstrate that H3.3B, depending on its chromatin localization, is involved in either up-regulation or down-regulation of expression of defined large chromatin regions.
Asunto(s)
Histonas , ARN Interferente Pequeño/metabolismo , Retroelementos , Espermatogénesis , Animales , Cromatina/genética , Histonas/genética , Histonas/metabolismo , Masculino , Ratones , Cromosomas Sexuales/metabolismoRESUMEN
The discoid shape of resting platelets is maintained by a peripheral, circular bundle of microtubules called marginal band. Marginal band microtubules are acetylated on lysine 40 of the alpha-tubulin subunits. We have previously shown that the deacetylase HDAC6 is responsible for tubulin deacetylation in platelets and that the hyperacetylated state of the microtubules in HDAC6KO platelets correlates with faster activation/spreading kinetics, pointing to a regulatory role of this modification. So far, the question about the reverse enzyme, responsible for tubulin acetylation in platelets, has remained unanswered. Several enzymes have been described as having tubulin acetylation activity. Here we identify αTAT1 as the enzyme responsible for the acetylation of marginal band microtubules. We show that αTAT1 deficiency has only minor consequences for platelet production and function. A residual tubulin acetylation level in αTAT1 deficient platelet lysates suggests the presence of an additional tubulin-acetylating enzyme that is unable to acetylate marginal band microtubules.
Asunto(s)
Acetiltransferasas/metabolismo , Microtúbulos/metabolismo , Animales , Humanos , RatonesRESUMEN
The conversion of male germ cell chromatin to a nucleoprotamine structure is fundamental to the life cycle, yet the underlying molecular details remain obscure. Here we show that an essential step is the genome-wide incorporation of TH2B, a histone H2B variant of hitherto unknown function. Using mouse models in which TH2B is depleted or C-terminally modified, we show that TH2B directs the final transformation of dissociating nucleosomes into protamine-packed structures. Depletion of TH2B induces compensatory mechanisms that permit histone removal by up-regulating H2B and programming nucleosome instability through targeted histone modifications, including lysine crotonylation and arginine methylation. Furthermore, after fertilization, TH2B reassembles onto the male genome during protamine-to-histone exchange. Thus, TH2B is a unique histone variant that plays a key role in the histone-to-protamine packing of the male genome and guides genome-wide chromatin transitions that both precede and follow transmission of the male genome to the egg.
Asunto(s)
Cromatina/metabolismo , Histonas/metabolismo , Protaminas/metabolismo , Animales , Epigénesis Genética , Femenino , Fertilización/fisiología , Regulación del Desarrollo de la Expresión Génica , Genoma , Histonas/genética , Masculino , Meiosis , Ratones , Nucleosomas , Espermatogénesis/genética , Testículo/metabolismoRESUMEN
Platelets, anucleated megakaryocyte (MK)-derived cells, play a major role in hemostasis and arterial thrombosis. Although protein kinase casein kinase 2 (CK2) is readily detected in MKs and platelets, the impact of CK2-dependent signaling on MK/platelet (patho-)physiology has remained elusive. The present study explored the impact of the CK2 regulatory ß-subunit on platelet biogenesis and activation. MK/platelet-specific genetic deletion of CK2ß (ck2ß-/- ) in mice resulted in a significant macrothrombocytopenia and an increased extramedullar megakaryopoiesis with an enhanced proportion of premature platelets. Although platelet life span was only mildly affected, ck2ß-/- MK displayed an abnormal microtubule structure with a drastically increased fragmentation within bone marrow and a significantly reduced proplatelet formation in vivo. In ck2ß-/- platelets, tubulin polymerization was disrupted, resulting in an impaired thrombopoiesis and an abrogated inositol 1,4,5-triphosphate receptor-dependent intracellular calcium (Ca2+) release. Presumably due to a blunted increase in the concentration of cytosolic Ca2+, activation-dependent increases of α and dense-granule secretion and integrin αIIbß3 activation, and aggregation were abrogated in ck2ß-/- platelets. Accordingly, thrombus formation and stabilization under high arterial shear rates were significantly diminished, and thrombotic vascular occlusion in vivo was significantly blunted in ck2ß-/- mice, accompanied by a slight prolongation of bleeding time. Following transient middle cerebral artery occlusion, ck2ß-/- mice displayed significantly reduced cerebral infarct volumes, developed significantly less neurological deficits, and showed significantly better outcomes after ischemic stroke than ck2ßfl/fl mice. The present observations reveal CK2ß as a novel powerful regulator of thrombopoiesis, Ca2+-dependent platelet activation, and arterial thrombosis in vivo.
Asunto(s)
Quinasa de la Caseína II/fisiología , Fragmentos de Péptidos/fisiología , Activación Plaquetaria , Trombopoyesis , Trombosis/patología , Animales , Plaquetas , Señalización del Calcio , Quinasa de la Caseína II/deficiencia , Megacariocitos/metabolismo , Megacariocitos/patología , Megacariocitos/ultraestructura , Ratones , Ratones Noqueados , Fragmentos de Péptidos/deficiencia , Trombosis/etiología , Trombosis/metabolismoRESUMEN
Male germ cell differentiation is a highly regulated multistep process initiated by the commitment of progenitor cells into meiosis and characterized by major chromatin reorganizations in haploid spermatids. We report here that a single member of the double bromodomain BET factors, Brdt, is a master regulator of both meiotic divisions and post-meiotic genome repackaging. Upon its activation at the onset of meiosis, Brdt drives and determines the developmental timing of a testis-specific gene expression program. In meiotic and post-meiotic cells, Brdt initiates a genuine histone acetylation-guided programming of the genome by activating essential genes and repressing a 'progenitor cells' gene expression program. At post-meiotic stages, a global chromatin hyperacetylation gives the signal for Brdt's first bromodomain to direct the genome-wide replacement of histones by transition proteins. Brdt is therefore a unique and essential regulator of male germ cell differentiation, which, by using various domains in a developmentally controlled manner, first drives a specific spermatogenic gene expression program, and later controls the tight packaging of the male genome.
Asunto(s)
Proteínas Nucleares/metabolismo , Espermatogénesis/fisiología , Animales , Perfilación de la Expresión Génica , Genoma/fisiología , Histona Acetiltransferasas/metabolismo , Histonas/metabolismo , Masculino , Meiosis/fisiología , Ratones , Espermatozoides/crecimiento & desarrollo , Espermatozoides/metabolismoRESUMEN
We report the identification of a new type of histone mark, lysine 2-hydroxyisobutyrylation (Khib), and identify the mark at 63 human and mouse histone Khib sites, including 27 unique lysine sites that are not known to be modified by lysine acetylation (Kac) and lysine crotonylation (Kcr). This histone mark was initially identified by MS and then validated by chemical and biochemical methods. Histone Khib shows distinct genomic distributions from histone Kac or histone Kcr during male germ cell differentiation. Using chromatin immunoprecipitation sequencing, gene expression analysis and immunodetection, we show that in male germ cells, H4K8hib is associated with active gene transcription in meiotic and post-meiotic cells. In addition, H4K8ac-associated genes are included in and constitute only a subfraction of H4K8hib-labeled genes. The histone Khib mark is conserved and widely distributed, has high stoichiometry and induces a large structural change. These findings suggest its critical role on the regulation of chromatin functions.
Asunto(s)
Histonas/metabolismo , Hidroxibutiratos/metabolismo , Lisina/metabolismo , Secuencia de Aminoácidos , Animales , Epigénesis Genética , Genoma , Células HeLa , Humanos , Hidroxibutiratos/química , Masculino , Espectrometría de Masas , Ratones , Datos de Secuencia Molecular , Espermatozoides/metabolismoRESUMEN
HDAC6, a major cytoplasmic deacetylase, is shown here to fine-tune the kinetics of platelet activation, a process that must be precisely regulated to ensure hemostasis after blood vessel injury while preventing pathologic thrombus formation. The discoid shape of resting platelets in the circulation is maintained by several highly acetylated microtubules organized in a marginal band. During platelet activation, microtubules undergo major reorganizations, which contribute to the shape change of activating platelets. We show that, during these activation-induced shape changes, a dramatic HDAC6-mediated tubulin deacetylation takes place, followed by microtubule reacetylation in spread platelets. In addition, although HDAC6-controlled tubulin deacetylation is not required for platelet activation, the capacity of HDAC6 to prevent tubulin hyperacetylation influences the speed of platelet spreading. These results are particularly important in view of HDAC6 inhibitors being currently used in clinical trials and represent the first example of cell signaling by lysine acetylation in platelet biology.
Asunto(s)
Histona Desacetilasas/fisiología , Activación Plaquetaria/fisiología , Acetilación , Secuencia de Aminoácidos , Animales , Plaquetas/efectos de los fármacos , Plaquetas/fisiología , Plaquetas/ultraestructura , Forma de la Célula , Tamaño de la Célula , Células Cultivadas/citología , Células Cultivadas/efectos de los fármacos , Histona Desacetilasa 6 , Inhibidores de Histona Desacetilasas/farmacología , Histona Desacetilasas/deficiencia , Histona Desacetilasas/genética , Humanos , Ácidos Hidroxámicos/farmacología , Ratones , Ratones Noqueados , Microtúbulos/metabolismo , Microtúbulos/ultraestructura , Datos de Secuencia Molecular , Activación Plaquetaria/efectos de los fármacos , Procesamiento Proteico-Postraduccional , Tubulina (Proteína)/metabolismoRESUMEN
Programs that govern stem cell maintenance and pluripotency are dependent on extracellular factors and of intrinsic cell modulators. Embryonic stem (ES) cells with a specific depletion of the gene encoding the regulatory subunit of protein kinase CK2 (CK2ß) revealed a viability defect. However, analysis of CK2ß functions along the neural lineage established CK2ß as a positive regulator for neural stem/progenitor cell (NSC) proliferation and multipotency. By using an in vitro genetic conditional approach, we demonstrate in this work that specific domains of CK2ß involved in the regulatory function towards CK2 catalytic subunits are crucial structural determinants for ES cell homeostasis.
Asunto(s)
Quinasa de la Caseína II/química , Quinasa de la Caseína II/metabolismo , Células Madre Embrionarias/enzimología , Animales , Dominio Catalítico , Diferenciación Celular , Linaje de la Célula , Proliferación Celular , Supervivencia Celular , Células Madre Embrionarias/citología , Ratones , Proteínas Mutantes/química , Proteínas Mutantes/metabolismo , Mutación/genética , Células-Madre Neurales/citología , Células-Madre Neurales/enzimología , Oligodendroglía/citología , Oligodendroglía/metabolismo , Relación Estructura-Actividad , Teratoma/patologíaRESUMEN
The molecular basis of residual histone retention after the nearly genome-wide histone-to-protamine replacement during late spermatogenesis is a critical and open question. Our previous investigations showed that in postmeiotic male germ cells, the genome-scale incorporation of histone variants TH2B-H2A.L.2 allows a controlled replacement of histones by protamines to occur. Here, we highlight the intrinsic ability of H2A.L.2 to specifically target the pericentric regions of the genome and discuss why pericentric heterochromatin is a privileged site of histone retention in mature spermatozoa. We observed that the intranuclear localization of H2A.L.2 is controlled by its ability to bind RNA, as well as by an interplay between its RNA-binding activity and its tropism for pericentric heterochromatin. We identify the H2A.L.2 RNA-binding domain and demonstrate that in somatic cells, the replacement of H2A.L.2 RNA-binding motif enhances and stabilizes its pericentric localization, while the forced expression of RNA increases its homogenous nuclear distribution. Based on these data, we propose that the specific accumulation of RNA on pericentric regions combined with H2A.L.2 tropism for these regions are responsible for stabilizing H2A.L.2 on these regions in mature spermatozoa. This situation would favor histone retention on pericentric heterochromatin.
Asunto(s)
Histonas/metabolismo , Proteínas con Motivos de Reconocimiento de ARN/metabolismo , ARN Nuclear/metabolismo , Espermatocitos/metabolismo , Espermatogénesis/genética , Animales , Núcleo Celular/metabolismo , Genoma Humano , Heterocromatina/metabolismo , Histonas/química , Histonas/genética , Humanos , Masculino , Ratones , Ratones Noqueados , Células 3T3 NIH , Proteínas con Motivos de Reconocimiento de ARN/química , Proteínas con Motivos de Reconocimiento de ARN/genética , Motivos de Unión al ARN , TransfecciónRESUMEN
BACKGROUND: Altering the biochemical makeup of chromatin by the incorporation of histone variants during development represents a key mechanism in regulating gene expression. The histone variant H2A.B, H2A.B.3 in mice, appeared late in evolution and is most highly expressed in the testis. In the mouse, it is encoded by three different genes. H2A.B expression is spatially and temporally regulated during spermatogenesis being most highly expressed in the haploid round spermatid stage. Active genes gain H2A.B where it directly interacts with polymerase II and RNA processing factors within splicing speckles. However, the importance of H2A.B for gene expression and fertility are unknown. RESULTS: Here, we report the first mouse knockout of this histone variant and its effects on fertility, nuclear organization, and gene expression. In view of the controversy related to the generation of off-target mutations by gene editing approaches, we test the specificity of TALENs by disrupting the H2A.B multi-copy gene family using only one pair of TALENs. We show that TALENs do display a high level of specificity since no off-target mutations are detected by bioinformatics analyses of exome sequences obtained from three consecutive generations of knockout mice and by Sanger DNA sequencing. Male H2A.B.3 knockout mice are subfertile and display an increase in the proportion of abnormal sperm and clogged seminiferous tubules. Significantly, a loss of proper RNA Pol II targeting to distinct transcription-splicing territories and changes to pre-mRNA splicing are observed. CONCLUSION: We have produced the first H2A.B knockout mouse using the TALEN approach.
Asunto(s)
Fertilidad/genética , Edición Génica/métodos , Histonas/genética , Infertilidad Masculina/etiología , Nucleasas de los Efectores Tipo Activadores de la Transcripción , Animales , Secuencia de Bases , Proteínas Cromosómicas no Histona/metabolismo , Femenino , Expresión Génica , Infertilidad Masculina/metabolismo , Infertilidad Masculina/patología , Masculino , Ratones Noqueados , Mutación , ARN Polimerasa II/metabolismo , Espermatozoides/metabolismo , Espermatozoides/patologíaRESUMEN
Dramatic and unique genome reorganizations accompany the differentiation of haploid male germ cells, characterized by a gradual loss of the vast majority of histones leading to a final tight compaction of the genome by protamines. Despite being essential for procreation and the life cycle, the mechanisms driving the transformation of nucleosomes into nucleoprotamines remain poorly understood. To address this issue, our laboratory has developed a number of specific approaches, ranging from the purification of spermatogenic cells at specific stages, the analysis of chromatin transitional states, the functional characterization of histone variants, histone-replacing proteins and their chaperones. This chapter will detail all related relevant techniques with a particular emphasis on methods allowing the functional studies of histone variants and the genome organizational states associated with the studied histones in spermatogenic cells undergoing histone-to-protamine exchange.
Asunto(s)
Genoma , Células Germinativas/citología , Células Germinativas/metabolismo , Meiosis , Animales , Histonas/metabolismo , Masculino , Meiosis/genética , Ratones , Nucleasa Microcócica/metabolismo , Proteínas Nucleares/aislamiento & purificación , Nucleosomas/metabolismo , Proteómica , Solubilidad , Espermátides/citología , Espermátides/metabolismoRESUMEN
Nuclear protein in testis (Nut) is a universal oncogenic driver in the highly aggressive NUT midline carcinoma, whose physiological function in male germ cells has been unclear. Here we show that expression of Nut is normally restricted to post-meiotic spermatogenic cells, where its presence triggers p300-dependent genome-wide histone H4 hyperacetylation, which is essential for the completion of histone-to-protamine exchange. Accordingly, the inactivation of Nut induces male sterility with spermatogenesis arrest at the histone-removal stage. Nut uses p300 and/or CBP to enhance acetylation of H4 at both K5 and K8, providing binding sites for the first bromodomain of Brdt, the testis-specific member of the BET family, which subsequently mediates genome-wide histone removal. Altogether, our data reveal the detailed molecular basis of the global histone hyperacetylation wave, which occurs before the final compaction of the male genome.
Asunto(s)
Histonas/metabolismo , Infertilidad Masculina/genética , Proteínas de Neoplasias/metabolismo , Proteínas Nucleares/metabolismo , Procesamiento Proteico-Postraduccional , Espermatozoides/metabolismo , Acetilación , Animales , Código de Histonas , Histonas/química , Masculino , Ratones , Proteínas de Neoplasias/genética , Proteínas Nucleares/genética , Unión Proteica , Espermatogénesis , Xenopus , Factores de Transcripción p300-CBP/metabolismoRESUMEN
Protein kinase CK2 is a ubiquitous protein kinase implicated in proliferation and cell survival. Its regulatory beta subunit, CK2beta, which is encoded by a single gene in mammals, has been suspected of regulating other protein kinases. In this work, we show that knockout of the CK2beta gene in mice leads to postimplantation lethality. Mutant embryos were reduced in size at embryonic day 6.5 (E6.5). They did not exhibit signs of apoptosis but did show reduced cell proliferation. Mutant embryos were resorbed at E7.5. In vitro, CK2beta(-/-) morula development stopped after the blastocyst stage. Attempts to generate homozygous embryonic stem (ES) cells failed. By using a conditional knockout approach, we show that lack of CK2beta is deleterious for mouse ES cells and primary embryonic fibroblasts. This is in contrast to what occurs with yeast cells, which can survive without functional CK2beta. Thus, our study demonstrates that in mammals, CK2beta is essential for viability at the cellular level, possibly because it acquired new functions during evolution.
Asunto(s)
Proteínas Serina-Treonina Quinasas/deficiencia , Animales , Blastocisto/citología , Quinasa de la Caseína II , División Celular , Supervivencia Celular , Desarrollo Embrionario y Fetal/genética , Desarrollo Embrionario y Fetal/fisiología , Femenino , Muerte Fetal/enzimología , Muerte Fetal/genética , Marcación de Gen , Edad Gestacional , Técnicas In Vitro , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Embarazo , Proteínas Serina-Treonina Quinasas/química , Proteínas Serina-Treonina Quinasas/genética , Subunidades de ProteínaRESUMEN
Isolation of pools of spermatogenic cells at specific developmental stages is essential for the investigations of molecular events controlling critical transitions during spermatogenesis. Large-scale cell purification techniques allow for combined proteomics, genomics, and transcriptomics studies. Herein, we describe a procedure for the purification of meiotic and post-meiotic male germ cells from adult mouse testes. We also describe how the fractionated cell populations could be used for further studies. In our laboratory, these protocols are routinely used to specifically investigate the molecular basis of histone acetylation/acylation-driven epigenetic programming.
Asunto(s)
Separación Celular/métodos , Epigénesis Genética , Histona Desacetilasas/genética , Histonas/genética , Espermátides/citología , Espermatocitos/citología , Animales , Centrifugación por Gradiente de Densidad , Inmunoprecipitación de Cromatina , Fluorocarburos/química , Inhibidores de Histona Desacetilasas/farmacología , Histona Desacetilasas/metabolismo , Histonas/metabolismo , Ácidos Hidroxámicos/farmacología , Masculino , Meiosis , Ratones , Albúmina Sérica Bovina/química , Espermátides/metabolismo , Espermatocitos/metabolismo , Espermatogénesis/genética , Testículo/citología , Testículo/crecimiento & desarrollo , Testículo/metabolismoRESUMEN
Protamines confer a compact structure to the genome of male gametes. Here, we find that somatic cells can be remodeled by transient expression of protamine 1 (Prm1). Ectopically expressed Prm1 forms scattered foci in the nuclei of fibroblasts, which coalescence into spermatid-like structures, concomitant with a loss of histones and a reprogramming barrier, H3 lysine 9 methylation. Protaminized nuclei injected into enucleated oocytes efficiently underwent protamine to maternal histone TH2B exchange and developed into normal blastocyst stage embryos in vitro. Altogether, our findings present a model to study male-specific chromatin remodeling, which can be exploited for the improvement of somatic cell nuclear transfer.
Asunto(s)
Núcleo Celular/metabolismo , Ensamble y Desensamble de Cromatina , Protaminas/metabolismo , Acetilación , Animales , Núcleo Celular/química , Células Cultivadas , Cromatina/metabolismo , ADN/química , ADN/metabolismo , Femenino , Fibroblastos/citología , Fibroblastos/metabolismo , Histonas/metabolismo , Humanos , Masculino , Metilación , Microscopía Electrónica de Transmisión , Oocitos/metabolismo , Protaminas/genética , Ovinos , Espermátides/química , Espermátides/metabolismoRESUMEN
AIMS: Ectopic activation of tissue-specific genes accompanies malignant transformation in many cancers. Prolactin (PRL) aberrant activation in lung cancer was investigated here to highlight its value as a biomarker. RESULTS: PRL is ectopically activated in a subset of very aggressive lung tumors, associated with a rapid fatal outcome, in our cohort of 293 lung tumor patients and in an external independent series of patients. Surprisingly PRL receptor expression was not detected in the vast majority of PRL-expressing lung tumors. Additionally, the analysis of the PRL transcripts in lung tumors and cell lines revealed systematic truncations of their 5' regions, including the signal peptide-encoding portions. PRL expression was found to sustain cancer-specific gene expression circuits encompassing genes that are normally responsive to hypoxia. Interestingly, this analysis also indicated that histone deacetylase (HDAC) inhibitors could counteract the PRL-associated transcriptional activity. INNOVATION AND CONCLUSION: Altogether, this work not only unravels a yet unknown oncogenic mechanism but also indicates that the specific category of PRL-expressing aggressive lung cancers could be particularly responsive to an HDAC inhibitor-based treatment.