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Appl Environ Microbiol ; 74(10): 3319-20, 2008 May.
Artículo en Inglés | MEDLINE | ID: mdl-18378648

RESUMEN

An assay based on transcription-mediated amplification (TMA) technology was used to quantitate Enterococcus fecal indicator bacteria in environmental water samples. The results generated by this and two growth-based methods relative to the 104 most-probable-number or CFU-per-100-ml threshold show that the three methods are in good qualitative agreement when tested against a range of water samples taken from different locations. The results demonstrate sensitive and rapid detection (approximately 4 h from sample collection to result) and quantitation of Enterococcus bacteria compared to the results with the growth-based methods.


Asunto(s)
Técnicas Bacteriológicas/métodos , Enterococcus/crecimiento & desarrollo , Enterococcus/aislamiento & purificación , Técnicas de Amplificación de Ácido Nucleico/métodos , Microbiología del Agua , Enterococcus/genética , Sensibilidad y Especificidad
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