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1.
Bioinformatics ; 38(6): 1724-1726, 2022 03 04.
Artículo en Inglés | MEDLINE | ID: mdl-34927668

RESUMEN

MOTIVATION: Tumor mutational burden (TMB) has been proposed as a predictive biomarker for immunotherapy response in cancer patients, as it is thought to enrich for tumors with high neoantigen load. TMB assessed by whole-exome sequencing is considered the gold standard but remains confined to research settings. In the clinical setting, targeted gene panels sampling various genomic sizes along with diverse strategies to estimate TMB were proposed and no real standard has emerged yet. RESULTS: We provide the community with TMBleR, a tool to measure the clinical impact of various strategies of panel-based TMB measurement. AVAILABILITY AND IMPLEMENTATION: R package and docker container (GPL-3 Open Source license): https://acc-bioinfo.github.io/TMBleR/. Graphical-user interface website: https://bioserver.ieo.it/shiny/app/tmbler. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.


Asunto(s)
Neoplasias , Humanos , Mutación , Neoplasias/patología , Inmunoterapia , Biomarcadores de Tumor/genética , Biología Computacional
2.
BMC Bioinformatics ; 21(1): 464, 2020 Oct 19.
Artículo en Inglés | MEDLINE | ID: mdl-33076821

RESUMEN

BACKGROUND: Genome browsers are widely used for locating interesting genomic regions, but their interactive use is obviously limited to inspecting short genomic portions. An ideal interaction is to provide patterns of regions on the browser, and then extract other genomic regions over the whole genome where such patterns occur, ranked by similarity. RESULTS: We developed SimSearch, an optimized pattern-search method and an open source plugin for the Integrated Genome Browser (IGB), to find genomic region sets that are similar to a given region pattern. It provides efficient visual genome-wide analytics computation in large datasets; the plugin supports intuitive user interactions for selecting an interesting pattern on IGB tracks and visualizing the computed occurrences of similar patterns along the entire genome. SimSearch also includes functions for the annotation and enrichment of results, and is enhanced with a Quickload repository including numerous epigenomic feature datasets from ENCODE and Roadmap Epigenomics. The paper also includes some use cases to show multiple genome-wide analyses of biological interest, which can be easily performed by taking advantage of the presented approach. CONCLUSIONS: The novel SimSearch method provides innovative support for effective genome-wide pattern search and visualization; its relevance and practical usefulness is demonstrated through a number of significant use cases of biological interest. The SimSearch IGB plugin, documentation, and code are freely available at https://deib-geco.github.io/simsearch-app/ and https://github.com/DEIB-GECO/simsearch-app/ .


Asunto(s)
Algoritmos , Epigenómica , Genoma , Reconocimiento de Normas Patrones Automatizadas , Navegador Web , Humanos , Anotación de Secuencia Molecular , Unión Proteica , Secuencias Reguladoras de Ácidos Nucleicos/genética , Factores de Transcripción/metabolismo
3.
BMC Bioinformatics ; 20(Suppl 4): 125, 2019 Apr 18.
Artículo en Inglés | MEDLINE | ID: mdl-30999855

RESUMEN

The 17th International NETTAB workshop was held in Palermo, Italy, on October 16-18, 2017. The special topic for the meeting was "Methods, tools and platforms for Personalised Medicine in the Big Data Era", but the traditional topics of the meeting series were also included in the event. About 40 scientific contributions were presented, including four keynote lectures, five guest lectures, and many oral communications and posters. Also, three tutorials were organised before and after the workshop. Full papers from some of the best works presented in Palermo were submitted for this Supplement of BMC Bioinformatics. Here, we provide an overview of meeting aims and scope. We also shortly introduce selected papers that have been accepted for publication in this Supplement, for a complete presentation of the outcomes of the meeting.


Asunto(s)
Biología Computacional/métodos , Atención a la Salud , Genómica , Humanos , Italia , Neoplasias/genética , Medicina de Precisión
4.
BMC Bioinformatics ; 17 Suppl 4: 54, 2016 Mar 02.
Artículo en Inglés | MEDLINE | ID: mdl-26961139

RESUMEN

BACKGROUND: Biologists generally interrogate genomics data using web-based genome browsers that have limited analytical potential. New generation genome browsers such as the Integrated Genome Browser (IGB) have largely overcome this limitation and permit customized analyses to be implemented using plugins. We illustrate the use of a plugin for IGB that exploits advanced visualization techniques to integrate the analysis of genomics data with network and structural approaches. RESULTS: We show how visualization technologies that combine both genomics and network biology can facilitate the selection of the key amino acid contacts from protein-protein and protein-drug interactions. Starting from the MDM2-P53 interaction, which is a high-value target for cancer therapy, and Nutlin, the parent small molecule of an MDM2 antagonist that is currently in clinical trials, we show that this method can be generalized to analyze how drugs and mutations can interfere with both protein-protein and drug-protein networks. We illustrate this point by two additional use-cases exploring the molecular basis of tamoxifen side effects and of drug resistance in chronic myeloid leukemia patients. CONCLUSIONS: Combined network and structure biology approaches provide key insights into both the genetic and the edgetic roles of variants in diseases. 3D interactomes facilitate the identification of disease-relevant interactions that can then be specifically targeted by drugs. Recent advances in molecular interaction and structure visualization tools have greatly simplified the mapping of mutated residues to molecular interaction interfaces. Such approaches can now also be integrated with genome visualization tools to enable comparative analyses of interaction contacts.


Asunto(s)
Gráficos por Computador , Redes Reguladoras de Genes/efectos de los fármacos , Genoma Humano , Mutación/genética , Preparaciones Farmacéuticas/metabolismo , Mapas de Interacción de Proteínas/efectos de los fármacos , Proteínas/metabolismo , Bases de Datos Factuales , Genómica/métodos , Humanos
5.
BMC Bioinformatics ; 17: 80, 2016 Feb 09.
Artículo en Inglés | MEDLINE | ID: mdl-26860319

RESUMEN

BACKGROUND: The increasing availability of resequencing data has led to a better understanding of the most important genes in cancer development. Nevertheless, the mutational landscape of many tumor types is heterogeneous and encompasses a long tail of potential driver genes that are systematically excluded by currently available methods due to the low frequency of their mutations. We developed LowMACA (Low frequency Mutations Analysis via Consensus Alignment), a method that combines the mutations of various proteins sharing the same functional domains to identify conserved residues that harbor clustered mutations in multiple sequence alignments. LowMACA is designed to visualize and statistically assess potential driver genes through the identification of their mutational hotspots. RESULTS: We analyzed the Ras superfamily exploiting the known driver mutations of the trio K-N-HRAS, identifying new putative driver mutations and genes belonging to less known members of the Rho, Rab and Rheb subfamilies. Furthermore, we applied the same concept to a list of known and candidate driver genes, and observed that low confidence genes show similar patterns of mutation compared to high confidence genes of the same protein family. CONCLUSIONS: LowMACA is a software for the identification of gain-of-function mutations in putative oncogenic families, increasing the amount of information on functional domains and their possible role in cancer. In this context LowMACA emphasizes the role of genes mutated at low frequency otherwise undetectable by classical single gene analysis. LowMACA is an R package available at http://www.bioconductor.org/packages/release/bioc/html/LowMACA.html. It is also available as a GUI standalone downloadable at: https://cgsb.genomics.iit.it/wiki/projects/LowMACA.


Asunto(s)
Análisis Mutacional de ADN/métodos , Mutación/genética , Neoplasias/genética , Neoplasias/metabolismo , Proteínas/metabolismo , Análisis de Secuencia de Proteína/métodos , Programas Informáticos , Humanos , Proteínas/genética
6.
Nat Methods ; 10(1): 47-53, 2013 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-23399932

RESUMEN

Network-centered approaches are increasingly used to understand the fundamentals of biology. However, the molecular details contained in the interaction networks, often necessary to understand cellular processes, are very limited, and the experimental difficulties surrounding the determination of protein complex structures make computational modeling techniques paramount. Here we present Interactome3D, a resource for the structural annotation and modeling of protein-protein interactions. Through the integration of interaction data from the main pathway repositories, we provide structural details at atomic resolution for over 12,000 protein-protein interactions in eight model organisms. Unlike static databases, Interactome3D also allows biologists to upload newly discovered interactions and pathways in any species, select the best combination of structural templates and build three-dimensional models in a fully automated manner. Finally, we illustrate the value of Interactome3D through the structural annotation of the complement cascade pathway, rationalizing a potential common mechanism of action suggested for several disease-causing mutations.


Asunto(s)
Modelos Biológicos , Complejos Multiproteicos/química , Mapeo de Interacción de Proteínas , Proteínas/química , Proteínas/metabolismo , Animales , Simulación por Computador , Bases de Datos de Proteínas , Humanos , Complejos Multiproteicos/metabolismo , Conformación Proteica
7.
Bioinformatics ; 31(22): 3679-81, 2015 Nov 15.
Artículo en Inglés | MEDLINE | ID: mdl-26209801

RESUMEN

UNLABELLED: Prioritization of candidate genes emanating from large-scale screens requires integrated analyses at the genomics, molecular, network and structural biology levels. We have extended the Integrated Genome Browser (IGB) to facilitate these tasks. The graphical user interface greatly simplifies building disease networks and zooming in at atomic resolution to identify variations in molecular complexes that may affect molecular interactions in the context of genomic data. All results are summarized in genome tracks and can be visualized and analyzed at the transcript level. AVAILABILITY AND IMPLEMENTATION: The MI Bundle is a plugin for the IGB. The plugin, help, video and tutorial are available at http://cru.genomics.iit.it/igbmibundle/ and https://github.com/CRUiit/igb-mi-bundle/wiki. The source code is released under the Apache License, Version 2. CONTACT: arnaud.ceol@iit.it SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.


Asunto(s)
Genoma , Genómica/métodos , Programas Informáticos , Subunidad alfa 2 del Factor de Unión al Sitio Principal/genética , Enfermedad/genética , Redes Reguladoras de Genes , Humanos
8.
Mol Cell Proteomics ; 13(5): 1318-29, 2014 May.
Artículo en Inglés | MEDLINE | ID: mdl-24627523

RESUMEN

Helicobacter pylori infections cause gastric ulcers and play a major role in the development of gastric cancer. In 2001, the first protein interactome was published for this species, revealing over 1500 binary protein interactions resulting from 261 yeast two-hybrid screens. Here we roughly double the number of previously published interactions using an ORFeome-based, proteome-wide yeast two-hybrid screening strategy. We identified a total of 1515 protein-protein interactions, of which 1461 are new. The integration of all the interactions reported in H. pylori results in 3004 unique interactions that connect about 70% of its proteome. Excluding interactions of promiscuous proteins we derived from our new data a core network consisting of 908 interactions. We compared our data set to several other bacterial interactomes and experimentally benchmarked the conservation of interactions using 365 protein pairs (interologs) of E. coli of which one third turned out to be conserved in both species.


Asunto(s)
Proteínas Bacterianas/metabolismo , Helicobacter pylori/metabolismo , Mapeo de Interacción de Proteínas/métodos , Mapas de Interacción de Proteínas , Secuencia de Aminoácidos , Secuencia Conservada , Sistemas de Lectura Abierta , Proteoma/análisis , Proteómica , Técnicas del Sistema de Dos Híbridos
9.
Nucleic Acids Res ; 42(Database issue): D374-9, 2014 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-24081580

RESUMEN

The database of 3D interacting domains (3did, available online for browsing and bulk download at http://3did.irbbarcelona.org) is a catalog of protein-protein interactions for which a high-resolution 3D structure is known. 3did collects and classifies all structural templates of domain-domain interactions in the Protein Data Bank, providing molecular details for such interactions. The current version also includes a pipeline for the discovery and annotation of novel domain-motif interactions. For every interaction, 3did identifies and groups different binding modes by clustering similar interfaces into 'interaction topologies'. By maintaining a constantly updated collection of domain-based structural interaction templates, 3did is a reference source of information for the structural characterization of protein interaction networks. 3did is updated every 6 months.


Asunto(s)
Bases de Datos de Proteínas , Dominios y Motivos de Interacción de Proteínas , Internet , Modelos Moleculares , Mapeo de Interacción de Proteínas , Mapas de Interacción de Proteínas
10.
BMC Bioinformatics ; 15 Suppl 14: S3, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-25471934

RESUMEN

BACKGROUND: Life-science laboratories make increasing use of Next Generation Sequencing (NGS) for studying bio-macromolecules and their interactions. Array-based methods for measuring gene expression or protein-DNA interactions are being replaced by RNA-Seq and ChIP-Seq. Sequencing is generally performed by specialized facilities that have to keep track of sequencing requests, trace samples, ensure quality and make data available according to predefined privileges. An integrated tool helps to troubleshoot problems, to maintain a high quality standard, to reduce time and costs. Commercial and non-commercial tools called LIMS (Laboratory Information Management Systems) are available for this purpose. However, they often come at prohibitive cost and/or lack the flexibility and scalability needed to adjust seamlessly to the frequently changing protocols employed. In order to manage the flow of sequencing data produced at the Genomic Unit of the Italian Institute of Technology (IIT), we developed SMITH (Sequencing Machine Information Tracking and Handling). METHODS: SMITH is a web application with a MySQL server at the backend. Wet-lab scientists of the Centre for Genomic Science and database experts from the Politecnico of Milan in the context of a Genomic Data Model Project developed SMITH. The data base schema stores all the information of an NGS experiment, including the descriptions of all protocols and algorithms used in the process. Notably, an attribute-value table allows associating an unconstrained textual description to each sample and all the data produced afterwards. This method permits the creation of metadata that can be used to search the database for specific files as well as for statistical analyses. RESULTS: SMITH runs automatically and limits direct human interaction mainly to administrative tasks. SMITH data-delivery procedures were standardized making it easier for biologists and analysts to navigate the data. Automation also helps saving time. The workflows are available through an API provided by the workflow management system. The parameters and input data are passed to the workflow engine that performs de-multiplexing, quality control, alignments, etc. CONCLUSIONS: SMITH standardizes, automates, and speeds up sequencing workflows. Annotation of data with key-value pairs facilitates meta-analysis.


Asunto(s)
Análisis de Secuencia de ADN/métodos , Programas Informáticos , Algoritmos , Automatización , Genómica , Secuenciación de Nucleótidos de Alto Rendimiento/instrumentación , Secuenciación de Nucleótidos de Alto Rendimiento/métodos , Análisis de Secuencia de ADN/instrumentación , Flujo de Trabajo
11.
BMC Bioinformatics ; 15 Suppl 1: S8, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-24564446

RESUMEN

BACKGROUND: Modern genomic technologies produce large amounts of data that can be mapped to specific regions in the genome. Among the first steps in interpreting the results is annotation of genomic regions with known features such as genes, promoters, CpG islands etc. Several tools have been published to perform this task. However, using these tools often requires a significant amount of bioinformatics skills and/or downloading and installing dedicated software. RESULTS: Here we present AnnotateGenomicRegions, a web application that accepts genomic regions as input and outputs a selection of overlapping and/or neighboring genome annotations. Supported organisms include human (hg18, hg19), mouse (mm8, mm9, mm10), zebrafish (danRer7), and Saccharomyces cerevisiae (sacCer2, sacCer3). AnnotateGenomicRegions is accessible online on a public server or can be installed locally. Some frequently used annotations and genomes are embedded in the application while custom annotations may be added by the user. CONCLUSIONS: The increasing spread of genomic technologies generates the need for a simple-to-use annotation tool for genomic regions that can be used by biologists and bioinformaticians alike. AnnotateGenomicRegions meets this demand. AnnotateGenomicRegions is an open-source web application that can be installed on any personal computer or institute server. AnnotateGenomicRegions is available at: http://cru.genomics.iit.it/AnnotateGenomicRegions.


Asunto(s)
Genómica/métodos , Animales , Genoma , Humanos , Internet , Ratones , Saccharomyces cerevisiae/genética , Programas Informáticos , Pez Cebra/genética
12.
Nucleic Acids Res ; 40(Web Server issue): W157-61, 2012 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-22618871

RESUMEN

The many ongoing genome sequencing initiatives are delivering comprehensive lists of the individual molecular components present in an organism, but these reveal little about how they work together. Follow-up initiatives are revealing thousands of interrelationships between gene products that need to be analyzed with novel bioinformatics approaches able to capture their complex emerging properties. Recently, we developed NetAligner, a novel network alignment tool that allows the identification of conserved protein complexes and pathways across organisms, providing valuable hints as to how those interaction networks evolved. NetAligner includes the prediction of likely conserved interactions, based on evolutionary distances, to counter the high number of missing interactions in current interactome networks, and a fast assessment of the statistical significance of individual alignment solutions, which increases its performance with respect to existing tools. The web server implementation of the NetAligner algorithm presented here features complex, pathway and interactome to interactome alignments for seven model organisms, namely Homo sapiens, Mus musculus, Drosophila melanogaster, Caenorhabditis elegans, Arabidopsis thaliana, Saccharomyces cerevisiae and Escherichia coli. The user can query complexes and pathways of arbitrary topology against a target species interactome, or directly compare two complete interactomes to identify conserved complexes and subnetworks. Alignment solutions can be downloaded or directly visualized in the browser. The NetAligner web server is publicly available at http://netaligner.irbbarcelona.org/.


Asunto(s)
Complejos Multiproteicos/metabolismo , Mapeo de Interacción de Proteínas/métodos , Programas Informáticos , Animales , Gráficos por Computador , Humanos , Internet , Ratones
14.
Nucleic Acids Res ; 39(Database issue): D718-23, 2011 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-20965963

RESUMEN

The database of three-dimensional interacting domains (3did) is a collection of protein interactions for which high-resolution three-dimensional structures are known. 3did exploits the availability of structural data to provide molecular details on interactions between two globular domains as well as novel domain-peptide interactions, derived using a recently published method from our lab. The interface residues are presented for each interaction type individually, plus global domain interfaces at which one or more partners (domains or peptides) bind. The 3did web server at http://3did.irbbarcelona.org visualizes these interfaces along with atomic details of individual interactions using Jmol. The complete contents are also available for download.


Asunto(s)
Bases de Datos de Proteínas , Dominios y Motivos de Interacción de Proteínas , Gráficos por Computador , Modelos Moleculares , Péptidos/química , Mapeo de Interacción de Proteínas
15.
J Biol Chem ; 286(6): 4173-85, 2011 Feb 11.
Artículo en Inglés | MEDLINE | ID: mdl-21123182

RESUMEN

There is growing evidence that tyrosine phosphatases display an intrinsic enzymatic preference for the sequence context flanking the target phosphotyrosines. On the other hand, substrate selection in vivo is decisively guided by the enzyme-substrate connectivity in the protein interaction network. We describe here a system wide strategy to infer physiological substrates of protein-tyrosine phosphatases. Here we integrate, by a Bayesian model, proteome wide evidence about in vitro substrate preference, as determined by a novel high-density peptide chip technology, and "closeness" in the protein interaction network. This allows to rank candidate substrates of the human PTP1B phosphatase. Ultimately a variety of in vitro and in vivo approaches were used to verify the prediction that the tyrosine phosphorylation levels of five high-ranking substrates, PLC-γ1, Gab1, SHP2, EGFR, and SHP1, are indeed specifically modulated by PTP1B. In addition, we demonstrate that the PTP1B-mediated dephosphorylation of Gab1 negatively affects its EGF-induced association with the phosphatase SHP2. The dissociation of this signaling complex is accompanied by a decrease of ERK MAP kinase phosphorylation and activation.


Asunto(s)
Sistema de Señalización de MAP Quinasas/fisiología , Proteína Tirosina Fosfatasa no Receptora Tipo 1/metabolismo , Proteoma/metabolismo , Proteínas Adaptadoras Transductoras de Señales/metabolismo , Línea Celular , Receptores ErbB/metabolismo , Quinasas MAP Reguladas por Señal Extracelular/metabolismo , Humanos , Fosfolipasa C gamma/metabolismo , Fosforilación/fisiología , Análisis por Matrices de Proteínas/métodos , Proteína Tirosina Fosfatasa no Receptora Tipo 11/metabolismo , Proteína Tirosina Fosfatasa no Receptora Tipo 6/metabolismo , Especificidad por Sustrato/fisiología
16.
Nucleic Acids Res ; 38(Database issue): D532-9, 2010 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-19897547

RESUMEN

MINT (http://mint.bio.uniroma2.it/mint) is a public repository for molecular interactions reported in peer-reviewed journals. Since its last report, MINT has grown considerably in size and evolved in scope to meet the requirements of its users. The main changes include a more precise definition of the curation policy and the development of an enhanced and user-friendly interface to facilitate the analysis of the ever-growing interaction dataset. MINT has adopted the PSI-MI standards for the annotation and for the representation of molecular interactions and is a member of the IMEx consortium.


Asunto(s)
Biología Computacional/métodos , Bases de Datos Genéticas , Bases de Datos de Ácidos Nucleicos , Mapeo de Interacción de Proteínas , Animales , Biología Computacional/tendencias , Bases de Datos de Proteínas , Receptores ErbB/metabolismo , Genoma Viral , Humanos , Almacenamiento y Recuperación de la Información/métodos , Internet , Lenguajes de Programación , Unión Proteica , Estructura Terciaria de Proteína , Programas Informáticos
17.
Nucleic Acids Res ; 37(Database issue): D669-73, 2009 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-18974184

RESUMEN

Understanding the consequences on host physiology induced by viral infection requires complete understanding of the perturbations caused by virus proteins on the cellular protein interaction network. The VirusMINT database (http://mint.bio.uniroma2.it/virusmint/) aims at collecting all protein interactions between viral and human proteins reported in the literature. VirusMINT currently stores over 5000 interactions involving more than 490 unique viral proteins from more than 110 different viral strains. The whole data set can be easily queried through the search pages and the results can be displayed with a graphical viewer. The curation effort has focused on manuscripts reporting interactions between human proteins and proteins encoded by some of the most medically relevant viruses: papilloma viruses, human immunodeficiency virus 1, Epstein-Barr virus, hepatitis B virus, hepatitis C virus, herpes viruses and Simian virus 40.


Asunto(s)
Bases de Datos de Proteínas , Mapeo de Interacción de Proteínas , Proteínas Virales/metabolismo , Gráficos por Computador
18.
Clin Lung Cancer ; 22(4): e637-e641, 2021 07.
Artículo en Inglés | MEDLINE | ID: mdl-33642178

RESUMEN

BACKGROUND: The deeper knowledge of non-small-cell lung cancer (NSCLC) biology and the discovery of driver molecular alterations have opened the era of precision medicine in lung oncology, thus significantly revolutionizing the diagnostic and therapeutic approach to NSCLC. In Italy, however, molecular assessment remains heterogeneous across the country, and numbers of patients accessing personalized treatments remain relatively low. Nationwide programs have demonstrated that the creation of consortia represent a successful strategy to increase the number of patients with a molecular classification. PATIENTS AND METHODS: The Alliance Against Cancer (ACC), a network of 25 Italian Research Institutes, has developed a targeted sequencing panel for the detection of genomic alterations in 182 genes in patients with a diagnosis of NSCLC (ACC lung panel). One thousand metastatic NSCLC patients will be enrolled onto a prospective trial designed to measure the sensitivity and specificity of the ACC lung panel as a tool for molecular screening compared to standard methods. RESULTS AND CONCLUSION: The ongoing trial is part of a nationwide strategy of ACC to develop infrastructures and improve competences to make the Italian research institutes independent for genomic profiling of cancer patients.


Asunto(s)
Carcinoma de Pulmón de Células no Pequeñas/diagnóstico , Neoplasias Pulmonares/diagnóstico , Carcinoma de Pulmón de Células no Pequeñas/genética , Detección Precoz del Cáncer , Genómica , Humanos , Italia , Neoplasias Pulmonares/genética , Tamizaje Masivo/métodos , Medicina de Precisión/métodos , Estudios Prospectivos , Sensibilidad y Especificidad
19.
Nat Biotechnol ; 25(8): 894-8, 2007 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-17687370

RESUMEN

A wealth of molecular interaction data is available in the literature, ranging from large-scale datasets to a single interaction confirmed by several different techniques. These data are all too often reported either as free text or in tables of variable format, and are often missing key pieces of information essential for a full understanding of the experiment. Here we propose MIMIx, the minimum information required for reporting a molecular interaction experiment. Adherence to these reporting guidelines will result in publications of increased clarity and usefulness to the scientific community and will support the rapid, systematic capture of molecular interaction data in public databases, thereby improving access to valuable interaction data.


Asunto(s)
Bases de Datos de Proteínas/normas , Guías como Asunto , Almacenamiento y Recuperación de la Información/normas , Mapeo de Interacción de Proteínas/normas , Proteómica/normas , Investigación/normas , Humanos , Internacionalidad
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