Effect of hot season on blood parameters, fecal fermentative parameters, and occurrence of Clostridium tyrobutyricum spores in feces of lactating dairy cows.

High temperature influences rumen and gut health, passage rate, and diet digestibility, with effects on fermentative processes. The main aim of the study was to investigate the effect of hot season on hindgut fermentation, the occurrence of Clostridium tyrobutyricum spores in bovine feces, and on their relationship with metabolic conditions in dairy cows producing milk used for Grana Padano cheese. The study was carried out on 7 dairy farms located in the Po Valley (Italy), involving 1,950 Italian Friesian dairy cows. The study was carried out from November 2013 till the end of July 2014. Temperature and relative humidity were recorded daily by weather stations. Constant management conditions were maintained during the experimental period. Feed and diet characteristics, metabolic conditions, and fecal characteristics were recorded in winter (from late November 2013 to the end of January 2014), spring (from April to May 2014), and summer (July 2014) season. In each season, blood samples were collected from 14 multiparous lactating dairy cows per herd to measure biochemical indices related to energy, protein, and mineral metabolism, as well as markers of inflammation and some enzyme activities. Fecal samples were also collected and measurements of moisture, pH and volatile fatty acids (VFA) were performed. The DNA extracted and purified from fecal samples was used to detect Clostridium tyrobutyricum spores in a quantitative real-time PCR assay. The daily mean temperature-humidity index was 40.7 ± 4.6 (range 25 to 55), 61.2 ± 3.7 (range 39 to 77), and 70.8 ± 3.2 (range 54 to 83) in winter, spring, and summer, respectively. Total VFA concentration in feces progressively decreased from winter to summer. The seasonal changes of acetate and propionate followed the same trend of total VFA; conversely, butyrate did not show any difference between seasons, and its molar proportion was greater in summer compared with winter. A greater occurrence of Cl. tyrobutyricum spores in summer compared with the other seasons was observed. The plasma concentrations of glucose, urea, albumin, Ca, Mg, Cl, Zn, and alkaline phosphatase activity were lower in summer compared with winter, whereas the opposite occurred for bilirubin and Na. Our results show that summer season, through direct and indirect effect of heat stress, affected fecal fermentative parameters and hindgut buffering capacity, and was responsible for the increasing occurrence of Cl. tyrobutyricum spores in feces.

Cooling systems of the resting area in free stall dairy barn.

A study during the summer season evaluated the effect of different cooling systems on behavioral and productive responses of Italian Friesian dairy cows kept in an experimental-free stall barn located in the Po Valley in Italy. The study involved 30 lactating dairy cows subdivided into two groups kept in two pens with external hard court paddock in each free stall. The same cooling system was applied in the feeding area in both pens. A different cooling system in the resting area was applied to the two pens: in the pen SW, the resting area was equipped with fans and misters; in the other, there was simple ventilation (SV). Breathing rate, rectal temperature, milk yield, and milk characteristics (fat, protein, and somatic cell count) were measured. Behavioral activities (standing and lying cows in the different areas, as well as the animals in the feed bunk) were recorded. Mild to moderate heat waves during the trial were observed. On average, the breathing rate was numerically greater in SV compared with SW cows (60.2 and 55.8 breath/min, respectively), and mean rectal temperature remained below 39 °C in both groups during the trial (on average 38.7 and 38.8 °C in SV and SW, respectively. During the hotter periods of the trial, the time spent lying indoor in the free stall was greater in SW (11.8 h/day) than SV (10.7 h/day). Conversely, the time spent standing indoor without feeding was greater in SV (4.3 h/day) than SW (3.8 h/day). Milk yield was slightly better maintained during hotter period in SW compared with SV and somatic cell count was also slightly greater in the former. In conclusion, the adoption of the cooling system by means of evaporative cooling also in the resting area reduces the alteration of time budget caused by heat stress.

Milk skimming, heating, acidification, lysozyme, and rennet affect the pattern, repeatability, and predictability of milk coagulation properties and of curd-firming model parameters: A case study of Grana Padano.

Milk coagulation properties are used to evaluate the cheesemaking aptitude of milk samples. No international standard procedure exists, although laboratories often mimic the production of a full-fat fresh cheese for milk coagulation properties. Questions have arisen about the predictability of such a procedure for different types of cheese production. The aim of this study was to establish a procedure mimicking the production conditions of a long-ripened hard cheese, taking Protected Designation of Origin Grana Padano as a case study. With respect to the traditional conditions (standard procedure; SP), the Grana Padano procedure (GP) modifications were the use of standardized milk, coagulation lower temperature, previous milk acidification, lysozyme addition, and rennet type. Each modification was tested in turn versus the SP and also all together in the GP. Another 3 tests were carried out: SP on naturally creamed milk, SP with double the quantity of rennet, and a simplified GP on a full-fat milk sample. The 10 procedures were tested on 2 subsamples with 2 replicates each and were repeated using individual milk samples from 15 dual-purpose Simmental cows in 4 sessions for a total of 600 tests. Two Formagraph instruments (Foss Electric A/S, Hillerød, Denmark) measuring curd firmness every 15 s were used, prolonging test duration to 60min to obtain 5 traditional single-point milk coagulation properties and 3 parameters of the curd firming model using all 240 points recorded for each replicate. The 8 traits of each replicate were analyzed according to a mixed model with fixed effects of 4 sessions, 10 treatments, 2 instruments, and 16microvats, and random effects of 15 animals and 300 subsamples. Compared with the SP, the coagulation and curd firming was slowed by low temperature and was accelerated by acidification and by adding a double amount of rennet; natural creaming, fat standardization, and rennet with 5% pepsin affected only some traits, whereas lysozyme addition affected none. Combination of all modifications tended to compensate for each of their effects, resulting in similar average patterns between GP and SP. Modifications to repeatability were found for all traits with the exception of 2. The ability of the SP to predict GP, tested through correlations between procedures, was not very high. Whereas SP is used for both research and in the dairy industry, better results for Grana Padano cheesemaking can only be achieved by adopting specific, more complex, and labor-intensive procedures at the research level or, possibly, by specific calibrations through Fourier-transform infrared spectroscopy at the industry level.

Effect of summer season on milk protein fractions in Holstein cows.

Milk characteristics are affected by heat stress, but very little information is available on changes of milk protein fractions and their relationship with cheesemaking properties of milk. The main objective of the study was to evaluate the effect of hot season on milk protein fractions and cheesemaking properties of milk for Grana Padano cheese production. The study was carried out in a dairy farm with a cheese factory for transforming the milk to Grana Padano cheese. The study was carried out from June 2012 to May 2013. Temperature and relative humidity of the inside barn were recorded daily during the study period using 8 electronic data loggers programmed to record every 30 min. Constant managerial conditions were maintained during the experimental periods. During the experimental period, feed and diet characteristics, milk yield, and milk characteristics were recorded in summer (from June 29 to July 27, 2012), winter (from January 25 to March 8, 2013), and spring (from May 17 to May 31, 2013). Milk yield was recorded and individual milk samples were taken from 25 cows selected in each season during the p.m. milking. Content of fat, proteins, caseins (CN), lactose and somatic cell count (SCC), titratable acidity, and milk rennet coagulation properties were determined on fresh samples. Milk protein fraction concentrations were determined by the sodium dodecyl sulfate-PAGE. Data were tested for nonnormality by the Shapiro-Wilk test. In case of nonnormality, parameters were normalized by log or exponential transformation. The data were analyzed with repeated measures ANOVA using a mixed model procedure. For all the main milk components (fat, protein, total solids, and solids-not-fat), the lowest values were observed in the summer and the greatest values were observed in the winter. Casein fractions, with the exception of γ-CN, showed the lowest values in the summer and the greatest values in the winter. The content of IgG and serum albumin was greater in summer than in the winter and spring. A mild effect of season was observed for milk SCC, with greater values in summer than in the winter and spring. A worsening of milk coagulation properties was observed in summer season. The alteration of cheesemaking properties during hot season seems strictly linked with changes of milk protein fractions mainly with the decrease of αS-CN and ß-CN and the increase of undefined proteins.

Rumination time around calving: an early signal to detect cows at greater risk of disease.

The main objective of this experiment was to evaluate the use of rumination time (RT) during the peripartum period as a tool for early disease detection. The study was carried out in an experimental freestall barn and involved 23 Italian Friesian cows (9 primiparous and 14 multiparous). The RT was continuously recorded by using an automatic system (Hr-Tag, SCR Engineers Ltd., Netanya, Israel), and data were summarized in 2-h intervals. Blood samples were collected from 30 d before calving to 42 d in milk (DIM) to assess biochemical indicators related to energy, protein, and mineral metabolism, as well as markers of inflammation and some enzyme activities. The liver functionality index, which includes some negative acute-phase proteins and related parameters (albumin, cholesterol, and bilirubin), was used to evaluate the severity of inflammatory conditions occurring around calving. The cows were retrospectively categorized according to RT observed between 3 and 6 DIM into those with the lowest (L) and highest (H) RT. The average RT before calving (-20 to -2d) was 479 min/d (range 264 to 599), reached a minimum value at calving (30% of RT before calving), and was nearly stable after 15 DIM (on average 452 min/d). Milk yield in early lactation (on average 26.8 kg/d) was positively correlated with RT (r = 0.33). After calving, compared with H cows, the L cows had higher values of haptoglobin (0.61 and 0.34 g/L at 10 DIM in L and H, respectively) for a longer time, had a greater increase in total bilirubin (9.5 and 5.7 µmol/L at 5 DIM in L and H), had greater reductions of albumin (31.2 and 33.5 g/L at 10 DIM in L and H) and paraoxonase (54 and 76 U/ml at 10 DIM in L and H), and had a slower increase of total cholesterol (2.7 and 3.2 mmol/L at 20 DIM in L and H). Furthermore, a lower average value of liver functionality index was observed in L (-6.97) compared with H (-1.91) cows. These results suggest that severe inflammation around parturition is associated with a slower increase of RT after calving. Furthermore, more than 90% of the cows in the L group had clinical diseases in early lactation compared with 42% of the H cows. Overall, our results demonstrate the utility of monitoring RT around calving, and in particular during the first week of lactation, as a way to identify in a timely fashion those cows at a greater risk of developing a disease in early lactation.

Selection for milk coagulation properties predicted by Fourier transform infrared spectroscopy in the Italian Holstein-Friesian breed.

Milk coagulation is based on a series of physicochemical changes at the casein micelle level, resulting in formation of a gel. Milk coagulation properties (MCP) are relevant for cheese quality and yield, important factors for the dairy industry. They are also evaluated in herd bulk milk to reward or penalize producers of Protected Designation of Origin cheeses. The economic importance of improving MCP justifies the need to account for this trait in the selection process. A pilot study was carried out to determine the feasibility of including MCP in the selection schemes of the Italian Holstein. The MCP were predicted in 1,055 individual milk samples collected in 16 herds (66 ± 24 cows per herd) located in Brescia province (northeastern Italy) by means of Fourier transform infrared (FTIR) spectroscopy. The coefficient of determination of prediction models indicated moderate predictions for milk rennet coagulation time (RCT=0.65) and curd firmness (a30=0.68), and poor predictions for curd-firming time (k20=0.49), whereas the range error ratio (8.9, 6.9, and 9.5 for RCT, k20, and a30, respectively) indicated good practical utility of the predictive models for all parameters. Milk proteins were genotyped and casein haplotypes (αS1-, ß-, αS2-, and κ-casein) were reconstructed. Data from 51 half-sib families (19.9 ± 16.4 daughters per sire) were analyzed by an animal model to estimate (1) the genetic parameters of predicted RCT, k20, and a30; (2) the breeding values for these predicted clotting variables; and (3) the effect of milk protein genotypes and casein haplotypes on predicted MCP (pMCP). This is the first study to estimate both genetic parameters and breeding values of pMCP, together with the effects of milk protein genotypes and casein haplotypes, that also considered k20, probably the most important parameter for the dairy industry (because it indicates the time for the beginning of curd-cutting). Heritability of predicted RCT (0.26) and k20 (0.31) were close to the average heritability described in literature, whereas the heritability of a30 was higher (0.52 vs. 0.27). The effects of milk proteins were statistically significant and similar to those obtained on measured MCP. In particular, haplotypes including uncommon variants showed positive (B-I-A-B) or negative (B-A(1)-A-E) effects. Based on these findings, FTIR spectroscopy-pMCP is proposed as a potential selection criterion for the Italian Holstein.

Rumination time during the summer season and its relationships with metabolic conditions and milk production.

The main objective of this experiment was to monitor the rumination pattern during the summer season in lactating dairy cows and to investigate its relationships with metabolic conditions and physiological markers of heat stress. The study was carried out in an experimental freestall barn located near Piacenza, Italy (45°01'N, 9°40'E; 68 m above sea level), and involved 21 Italian Friesian cows (11 primiparous and 10 multiparous) during the summer season. Rumination time (RT) was recorded by using an automatic system and data were calculated and summarized in 2-h intervals. Microclimatic conditions (temperature and relative humidity) inside the barn were recorded during the trial, and the temperature-humidity index (THI) was calculated. Breathing rates and rectal temperatures were recorded following stable meteorological periods characterized by lower and higher temperatures. At the same times, blood samples were collected to assess biochemical variables related to energy, protein, and mineral metabolism, as well as markers of inflammatory conditions and enzyme activity. Daily milk yield, body weight, nutritional condition, and health status were also recorded. The average RT was 501 min/d, with no significant differences between primiparous and multiparous cows. According to the microclimatic conditions and physiological markers of heat stress, the cows suffered mild to moderate heat stress during the summer. A negative relationship between daily maximum THI and RT was observed (r=-0.32), with a reduction of 2.2 min of RT for every daily maximum THI unit over the threshold of daily maximum THI of 76. Most of the rumination occurred during the night (on average the nighttime RT was 63.2% of daytime and nighttime RT); moreover, the proportion of nighttime RT slightly but significantly increased as THI increased. Rumination time throughout the trial was negatively related to breathing rate and positively related to milk yield. Daily maximum THI was negatively correlated with plasma glucose (r=-0.52) and positively correlated with plasma ß-hydroxybutyric acid (r=0.26). Values of plasma ß-hydroxybutyric acid were positively related to RT through the trial. Our results indicate that hot conditions negatively affect RT and modify its daily pattern. The relationship between RT and the physiological markers used in our trial support the use of RT as a marker of heat stress.

Effects of different feeding time and frequency on metabolic conditions and milk production in heat-stressed dairy cows.

The aim of this paper was to evaluate the effects of three different feeding management (FM) schedules on physiological markers of heat stress (HS), metabolic conditions, milk yield and quality during the hot season in dairy cows. The study involved 27 mid-lactating cows, subdivided in three homogeneous groups differing in feeding time and frequency: total mixed ration (TMR) delivered once daily in the morning (M); twice daily, half in the morning and half in the evening (ME); once daily in the evening (E). During the trial, blood samples were collected in the morning (a.m.) and in the evening (p.m.), breathing rate (BR), rectal temperature (RT), and milk yield were recorded and individual milk samples were collected. Microclimate data indicated that cows were subjected to mild-moderate HS. During the hotter days, cows receiving M treatment showed higher values of RT (38.97 °C vs 38.68 °C and 38.62 °C, in ME and E) and BR (71.44 vs 66.52 and 65.26 breaths min⁻¹, in ME and E), a.m. plasma glucose was lower in M (3.69 vs 3.83 and 3.83 mmol L⁻¹, in ME and E) and a.m. plasma urea was lower in E (4.82 vs 5.48 and 5.35 mmol L⁻¹, in M and ME). Milk yield was unaffected by FM, as well as milk composition and cheese-making properties. Only milk protein content and yield were higher in M (3.42 vs 3.36 and 3.27 g 100 mL⁻¹; and 1.11 vs 1.08 and 1.02 kg day⁻¹, for ME and E). Our results on cow physiology indicate that M seems a less suitable FM to match cow welfare during the summer season.

Plasma paraoxonase, health, inflammatory conditions, and liver function in transition dairy cows.

Paraoxonase (PON) is a liver protein with hydrolase activity that is released into the blood stream. Paraoxonase may serve as an index of liver function because it is drastically reduced in chronic liver damage. Sixty-seven periparturient dairy cows were used to evaluate the relationship between plasma PON, health problems, inflammatory conditions, and liver function. Baseline plasma PON concentrations during the first 30 d in milk (DIM) were retrospectively used to group cows into quartiles. Metabolic profile, lipid metabolites (e.g., nonesterified fatty acids, beta-hydroxybutyrate), inflammatory indices (haptoglobin, ceruloplasmin), low and high density lipoprotein cholesterol, vitamin A, vitamin E, reactive oxygen metabolites, total antioxidants, and PON in plasma were measured 2 wk before to 8 wk after calving. Weekly milk yield, body condition score, and all health problems were recorded. After parturition (7 DIM), cows in the lower PON group had the lowest plasma concentrations of negative acute phase proteins compared with the higher PON group for retinol binding protein (23.2 +/- 2.86 vs. 36.0 +/- 2.96 microg/dL of vitamin A), albumin (31.6 +/- 0.73 vs. 33.9 +/- 0.75 g/L), total cholesterol (2.04 +/- 0.30 vs. 2.45 +/- 0.42 mmol/L), and the highest concentrations of haptoglobin (0.67 vs. 0.24 +/- 0.03 g/L; positive acute phase protein) and globulins (37.2 vs. 32.3 +/- 1.4 g/L). Plasma bilirubin was highest in the cows (10.1 vs. 6.2 +/- 0.6 micromol/L) in the lowest PON quartile. Plasma PON was negatively correlated with haptoglobin (r = -0.39) and bilirubin (r = -0.42) and positively correlated with retinol binding protein (r = 0.54), albumin (r = 0.38), and cholesterol (r = 0.55) fractions. A total of 82.3% of cows in the lower quartile and no cows in the upper quartile experienced serious inflammation. Lower quartile cows produced 28.1 +/- 10.3 kg of milk/d; whereas upper quartile cows produced 38.3 +/- 7.7 kg of milk/d during the first 30 DIM. A reduction in the ability of the liver to cope with the increased metabolic demand near parturition in dairy cows can be diagnosed using changes in baseline plasma PON.

Welfare assessment based on metabolic and endocrine aspects in primiparous cows milked in a parlor or with an automatic milking system.

An automatic milking system (AMS) was compared with a traditional milking parlor (MP) to evaluate metabolic and psycho-physiological aspects of animal welfare. Twenty Italian Friesian heifers were allocated to 2 groups of 10 cows each after calving and maintained in the same free-stall barn. The first group was milked twice daily in a MP; the second group was milked in a single box AMS. Feed and diet characteristics were analyzed. Health status and body condition score (BCS) were evaluated in each cow. Blood samples were obtained from -14 to 154 d in milk (DIM) to determine metabolic profile and basal concentrations of cortisol in plasma. Data collected from 10 cows per group were processed. No significant difference was detected in milk yield, BCS, and energy-related metabolites (glucose, nonesterified fatty acids, beta-hydroxybutyrate, and triglycerides) from cows in MP or in AMS during the first 22 wk of lactation. These results, jointly with the absence of significant differences in plasma metabolites related to protein metabolism, mineral metabolism, and liver function during the first 22 wk of lactation, indicates that cows in AMS did not suffer metabolically. Greater basal concentrations of plasma cortisol in AMS cows, even if absolute values were considered to be in an acceptable range, might indicate chronic stress in these primiparous cows. Further research is necessary to confirm this hypothesis.

Pattern of γ-glutamyl transferase activity in cow milk throughout lactation and relationships with metabolic conditions and milk composition.

The main objective of this experiment was to study the γ-glutamyl transferase (GGT) activity in milk during lactation and its relationship with metabolic status of dairy cows, milk yield, milk composition, and cheesemaking properties. The study was performed in a tied stall barn and involved 20 lactations from 12 healthy multiparous Italian Friesian dairy cows. During lactation starting at d 10, milk samples were collected weekly and analyzed for composition, somatic cells count, titratable acidity, and milk coagulation properties. The GGT activity was measured in defatted samples. Blood samples were collected weekly to assess biochemical indicators related to energy, protein, and mineral metabolism, markers of inflammation and some enzyme activities. The lactations of each cow were retrospectively categorized into 2 groups according to their milk GGT activity value through lactation. A median value of GGT activity in the milk of all lactations was calculated (3,045 U/L), and 10 lactations with lower GGT activity were classified as low while 10 lactations with greater GGT activity were classified as high. The average value of milk GGT activity during lactation was 3,863 and 3,024 U/L for high and low, respectively. The GGT activity decreased in early lactation and reached minimum values in the second month (3,289 and 2,355 U/L for high and low, respectively). Thereafter GGT activity increased progressively, reaching values in late lactation of 4,511 and 3,540 U/L in high and low, respectively. On average, milk yield was 40.81 and 42.76 kg/d in high and low, respectively, and a negative partial correlation with milk GGT activity was observed. A greater milk protein concentration was observed in high (3.39%) compared with low (3.18%), and a positive partial correlation with milk GGT activity was observed. Greater titratable acidity in high than that in low (3.75 vs. 3.45 degrees Soxhlet-Henkel/50 mL, respectively) was also observed. Plasma glucose was greater in cows of high than in low group, while plasma urea was lower in the high than in the low group. No relationship between plasma GGT and milk GGT activity was observed. Our results show an important effect of lactation stage on milk GGT activity. The individual effect observed from consecutive lactations and the relationship between milk GGT activity and milk protein concentration in healthy cows could open prospects for GGT as a future tool in improving milk protein content.

Relationships between rumination time, metabolic conditions, and health status in dairy cows during the transition period.

The main objective of this experiment was to monitor the rumination pattern during the transition period in primiparous (PR) and pluriparous (PL) dairy cows and to investigate its relationships with metabolic conditions, milk yield, and health status. The study was carried out in an experimental free-stall barn and involved 32 Italian Friesian cows (9 PR and 23 PL) during the transition phase. The rumination time (RT) was recorded with an automatic system (HR-Tag), and data were calculated and summarized in 2-h intervals. Blood samples were collected during the transition phase to assess biochemical variables related to energy, protein, and mineral metabolism, as well as markers of inflammatory conditions and some enzyme activity. Daily milk yield, BW, nutritional condition, and health status were also recorded. The average RT before calving (-20 to -6 d) was 463 min/d in PR (range 270 to 620) and 522 min/d in PL (range 411 to 640). In the early lactation [15 to 40 d in milk (DIM)], the average RT was 504 min/d in PR (range 400 to 585) and 562 min/d in PL (range 414 to 685) and was positively correlated with milk yield (r = 0.36; P < 0.001). The RT reached the minimum at calving d (262 min/d in PR and 278 min/d in PL). Before calving the percentage of RT during the nighttime was 60% in PR and 62% in PL, whereas the values decreased after calving (55% in PR and 57% in PL). During the first weeks of lactation, PR showed a shorter RT than PL in the 2-h intervals that included milkings. Cows with reduced RT before calving maintained reduced RT after calving and suffered a greater frequency of disease than cows with greater RT in late pregnancy. Moreover, cows characterized by mild inflammatory conditions and without health disorders or only mild health disorders during the puerperium showed a greater average rumination time (over 520 min/d) during the first 10 d of lactation. Conversely, the decreased RT (450 min/d) during the first few days of lactation was observed in cows with subclinical diseases or health disorders. Cows affected by clinical mastitis during the trial showed a reduction of RT and a change in its variability already some days before the drug treatment. Our results suggest that the automatic measurement of RT is useful to predict calving time and to quickly obtain information on health status of the animals in a period as critical as the transition phase.

Metabolic and hematological profiles in mature horses supplemented with different selenium sources and doses.

This study was conducted to determine the effects of dietary Se source and dose on metabolic and hematological profiles, and their relationships with oxidative status in horses. Twenty-five mature horses were blocked by BW and randomly allocated to 1 of 5 dietary treatments: negative control (CTRL: 0.085 mg of Se/kg of DM), 3 different dietary concentrations of organic Se provided by Se yeast (SY02, SY03, and SY04 containing 0.2, 0.3, and 0.4 mg of total Se/kg of DM, respectively), and 1 positive control provided by sodium selenite (SS03 containing 0.3 mg of total Se/kg of DM). Horses were fed the same basal diet (6 kg of grass hay and 3 kg of concentrate per horse daily) and received their respective treatments for a continuous period of 112 d. Jugular venous blood samples were collected before the morning feed on d 0, 28, 56, 84, and 112. Whole blood was analyzed for hematological profile, and plasma was analyzed for metabolites of energy, protein, and mineral metabolism; enzymatic activities and metabolites related to liver and muscle damage; and markers of inflammatory and oxidative status. Plasma metabolites related to energy, protein, and mineral metabolism, acute phase proteins, and enzyme activities related to hepatocellular, hepatobiliary, and muscle damage were not affected by Se source or dose. There were no differences among treatments in either reactive oxygen metabolites or thiol group concentrations in plasma. However, a linear decrease (P < 0. 01) in plasma total antioxidants was observed with increasing Se yeast supplementation. Furthermore, total antioxidant concentrations were less in SY03 than SS03 horses (P < 0.05), and were less in SY03 and SY04 than CTRL horses (P < 0.05). These results could be interpreted as an improvement in the preventive antioxidant systems of horses fed Se yeast. Total white blood cell count was not affected by treatment. There was a tendency for horses receiving greater concentrations of Se yeast to have greater lymphocyte counts (P = 0.09), with greater lymphocyte counts in blood of SY03 vs. SS03 horses (P < 0.05). Despite the lack of effect of Se source and dose on markers of inflammatory and liver status, the hematological profile seems to indicate an immunomodulatory action, as shown by mild changes in the white blood cell populations in response to Se yeast inclusion.

Effect of selenium source and dose on selenium status of mature horses.

This study was conducted to determine the effects of either dietary Se source or dose on the Se status of horses. Twenty-five mature horses were blocked by BW and randomly allocated to 1 of 5 dietary treatments that comprised the same basal diet that differed only in Se source or dose. Treatments were as follows: negative control (0.085 mg of Se/kg of DM), 3 different dietary concentrations of supplemental organic Se (Se yeast; 0.2, 0.3, and 0.4 mg of total Se/kg of DM), and positive control (0.3 mg of total Se/kg of DM) supplemented with Na selenite. Horses initially received the control diet (6 kg of grass hay and 3 kg of concentrate per horse daily) for 56 d to allow diet adaptation. After the period of diet adaptation, horses were offered their respective treatments for a continuous period of 112 d. Jugular venous blood samples were collected before the morning feed on d 0, 28, 56, 84, and 112. Whole blood and plasma were analyzed for total Se, glutathione peroxidase activity in whole blood (GPX-1) and plasma, and thyroid hormones (thyroxine and triiodothyronine) in plasma. The proportion of total Se as selenomethionine (SeMet) or selenocysteine in pooled whole blood and plasma samples was determined on d 0, 56, and 112. Data were analyzed as repeated measures. Total Se in blood and plasma and GPX-1 activity were greater in all supplemented horses (P < 0.001, except P < 0.01 for GPX-1 in horses supplemented with the least dose of Se yeast) with a linear dose effect of Se yeast for whole blood and plasma Se (P < 0.001) and a quadratic dose effect (P < 0.05) for whole blood GPX-1 activity. A plateau for total Se in plasma was achieved within 75 to 90 d, although this was not observed in blood total Se or GPX-1 activity. On d 84 and 112, horses supplemented with Se yeast showed greater total Se in blood (P < 0.05) compared with horses supplemented with Na selenite, and a source effect (P < 0.05) was observed in the relationship between total blood Se and GPX-1 activity. Selenocysteine (the predominant form of Se in whole blood and plasma) increased in all horses supplemented with Se. The SeMet content of whole blood and plasma increased in horses supplemented with Se yeast, but it was not observed in those supplemented with selenite. The rate of increase in SeMet over time was greater in whole blood (P < 0.05) and plasma (P = 0.10) with the Se yeast product. In conclusion, Se yeast was more effective than Na selenite in increasing total Se in blood, mainly as consequence of a greater increase of the proportion of Se comprised as SeMet, but it did not modify GPX-1 activity.

Effects of daily gain in pre- and postpubertal replacement dairy heifers on body condition score, body size, metabolic profile, and future milk production.

Two trials were conducted to evaluate the effect of moderate (0.7 kg) and accelerated (0.9 kg) average daily gain before (trial 1) and after (trial 2) puberty on body condition, metabolic profile, and first lactation milk production of Italian Holstein-Friesian heifers. There were 20 heifers in trial 1 and 22 in trial 2. Trials started when heifers averaged 150 and 300 kg of body weight in trial 1 and 2, respectively, and lasted 7 mo (experimental period). Across diet groups, half of the heifers were mated at first estrus after 370 kg and the other half after 420 kg of body weight gain. Actual average daily gains were 0.667 and 0.775 kg in trial 1 and 0.748 and 0.824 kg in trial 2 for moderate and accelerated experimental groups, respectively. Diets for high average daily gain did not affect body condition during growing phase in trial 1, whereas it did in trial 2. High average daily gain increased plasma glucose in trial 1 and plasma urea concentration in trial 2. Rearing diet did not affect milk production and milk protein percent; age in both trials. High average daily gain decreased milk fat percentage in trial 2. Early calving negatively influenced milk production in both trials and milk fat percentage in trial 1. Early calving heifers showed higher protein percentage than those with late calving only in trial 1.

Producing specific milks for speciality cheeses.

Protected denomination of origin (PDO) cheeses have distinctive sensorial characteristics. They can be made only from raw milk possessing specific features, which is processed through the 'art' of the cheesemaker. In general, the distinctive sensorial traits of PDO cheese cannot be achieved under different environmental-production conditions for two main reasons: (1) some milk features are linked to specific animal production systems; (2) cheese ripening is affected by the interaction between milk (specific) and the traditional technology applied to the transformation process (non-specific). Also, the environment for a good ripening stage can be quite specific and not reproducible. With reference to milk, factors of typicality are species and/or breed, pedoclimatic conditions, animal management system and feeding. Other factors that influence cheese quality are milk treatments, milk processing and the ripening procedures. The technology applied to most cheeses currently known as PDO utilizes only raw milk, rennet and natural lactic acid bacteria, so that milk must be, at its origin, suitable for processing. The specific milk characteristics that ensure a high success rate for PDO cheeses are high protein content and good renneting properties, appropriate fat content with appropriate fatty acid composition and the presence of chemical flavours originating from local feeds. Moreover, an appropriate microflora is also of major importance. The factors that contribute to achieving milk suitable for transformation into PDO cheese are genetics, age, lactation stage, season and climate, general management and health conditions, milking and particularly feeding, which affect nutrient availability, endocrine response and health status, and also the presence of microbes and chemical substances which enrich or reduce the milk-cheese quality. Many of these factors are regulated by the Producer Associations. However, the secret of the success of PDO cheeses is the combination of modern technology and tradition, with the objective of adapting the product to market demand, without losing specificity, originality and authenticity.