Assessing the Potential Apoptotic Effects of Different Hydatid Cyst Fluids on Human Healthy Hepatocytes and Hepatocellular Carcinoma Cells.

Cystic Echinococcosis (CE) is a zoonotic infection caused by the larval form of Echinococcus granulosus in humans. Emerging evidence suggests an intriguing inverse association between E. granulosus infection and the occurrence of cancer. This study aimed to investigate the influence of diverse host-derived hydatid cyst fluids (HCF) with distinct genotypes on human liver hepatocytes (HC) and hepatocellular carcinoma cells (HepG2). Specifically, we examined their effects on cell proliferation, apoptosis sensitivity (BAX/BCL-2), apoptosis-related p53 expression, and the expression of cancer-related microRNA (hsa-miR-181b-3p). Cell proliferation assays, real-time PCR, and ELISA studies were conducted to evaluate potential anti-cancer properties. The findings revealed that animal-origin HCF (G1(A)) induced direct cell death by augmenting the susceptibility of HepG2 cells to apoptosis. Treatment with both G1(A) and G1(H) HCF sensitized HepG2 and HC cell lines to apoptosis by modulating the BAX/BCL-2 ratio, accompanied by upregulation of the p53 gene. Additionally, G1(A) HCF and human-derived HCFs (G1(H), G7(H)) reduced the expression of miR-181b-3p in HepG2 cells. Consequently, this study demonstrates the potential anti-cancer effect of HCF in HepG2 cells and provides the first comparative assessment of HCFs from human and animal sources with diverse genotypes, offering novel insights into this field.

Comparison of molecular and morphological characterization and haplotype analysis of cattle and sheep isolates of cystic echinococcosis.

Cystic echinococcosis is a zoonotic disease and the second most common foodborne parasitic infection worldwide. The aim of the present study was to investigate genetic variations in G1 and G3 genotypes of Echinococcus granulosus sensu stricto and determine the morphological differences between two genotypes. In total, 119 cystic samples were collected from 48 cattle and 71 sheep in slaughterhouses in four cities in three geographical regions of Turkey regions (Ankara, Central Anatolio region; Ordu, Black Sea region; and Adana, Mersin, Mediterranean region). For molecular characterization of the G1 and G3 genotypes, two gene regions (the complete mt-cox1 gene sequence and partial mt-nad5 gene sequence) were amplified. Haplotype analysis was conducted to determine the nucleotide differences between the complete sequences of the mt-cox1 gene for 47 samples. In addition, morphological parameters in protoscoleces of fertile cysts were measured to determine the relationship between the genotypes and morphometry. According to the obtained genotype and morphometry results, there were no statistically significant differences between the genotypes in terms of the number of hooks, total lengths of large and small hooks, blade lengths of large and small hooks, and widths of small hooks, although there was a statistically significant difference in large hook width (p > 0.05).

Molecular and Morphological Characterization of Cysticercus tenuicollis in Red Deer (Cervus elaphus) from Turkey.

INTRODUCTION: Cysticercus tenuicollis, the metacestode stage of Taenia hydatigena has a worldwide distribution and is particularly common in rural areas and developing countries. In rare cases, T. hydatigena infection may result in the death of its host due to severe damage or secondary bacterial infections. Generally, when multiple cysts are attached to the liver, it causes economic losses. The infection can be detected using morphological, histopathological and more recently, molecular investigations. METHODS: In the present study, we describe molecular and morphological characterization of C. tenuicollis detected in a 3 month-old female red deer (Cervus elaphus) during necropsy. Cystic samples were stained with haematoxylin-eosin (HE) and Masson's trichrome stain for histopathological examination, and molecular characterization of the complete mitochondrial cytochrome c oxidase subunit 1 (cox1) gene region was performed. PURPOSE: This study provides the first morphological, histopathological, and molecular data on C. tenuicollis isolated from red deer in Turkey.