Asunto(s)
Antígenos/análisis , Proteínas/análisis , Fracciones Subcelulares/inmunología , Trypanosoma cruzi/inmunología , Animales , Electroforesis en Gel de Poliacrilamida , Epítopos , Inmunoelectroforesis , Microsomas/ultraestructura , Mitocondrias/ultraestructura , Trypanosoma cruzi/ultraestructuraAsunto(s)
Amígdala del Cerebelo/cirugía , Branquioma/cirugía , Cuello/cirugía , Adulto , Niño , Humanos , Métodos , Persona de Mediana EdadRESUMEN
Local infection is a rare complication after aesthetic rhinoplasty. In the past serious complications and 1 death have been reported. We present 5 cases of local infection after primary and secondary procedures. Infection seems to have more to do with technical details during an operation, such as the external lateral osteotomy, than with the presence of saprophytic bacteria. Care must be taken in the management of cartilage grafts. There is not enough support for the use of prophylactic antibiotics. Usually the organism implicated is Staphylococcus aureus. Once the infection has developed, treatment should be aggressive in view of the grave complications reported in the literature.
Asunto(s)
Rinoplastia/efectos adversos , Infecciones Estafilocócicas/etiología , Infección de la Herida Quirúrgica/etiología , Adolescente , Adulto , Antibacterianos/administración & dosificación , Femenino , Humanos , Masculino , Rinoplastia/métodos , Infecciones Estafilocócicas/tratamiento farmacológico , Infecciones Estafilocócicas/microbiología , Staphylococcus aureus/aislamiento & purificación , Infección de la Herida Quirúrgica/tratamiento farmacológico , Infección de la Herida Quirúrgica/microbiologíaRESUMEN
A method for isolation of membrane and flagellar fractions from Trypanosoma cruzi epimastigotes, and descriptions of their ultrastructural characteristics and antigenic activity are presented. Electron-microscopic observation revealed that the membrane fraction resembled vesicles, as occurs frequently when the pressure-depressure method is used for cell disruption. The flagellar fraction revealed flagella and some membranes, both related and unrelated to the flagellar structure. Flagellar and membrane fractions had 5 and 3 precipitin lines respectively against anti-whole homogenate of T. cruzi, in double diffusion tests. One of the precipitin bands common to both fractions gave a reaction of identiy with one precipitin line of the microsomal fractions, that was not present in the mitochondrial, nuclear and cell sap fractions. Data from tests of protective activity activity obtained during a period of 3 years with whole homogenate and F and M fraction are also presented Protective activity against lethal challenge doses of trypomastigotes is strongly associated with the flagellar fraction.