1.
Nat Methods
; 9(10): 969-72, 2012 Oct.
Artículo
en Inglés
| MEDLINE
| ID: mdl-22961246
RESUMEN
Regulatory DNA elements can control the expression of distant genes via physical interactions. Here we present a cost-effective methodology and computational analysis pipeline for robust characterization of the physical organization around selected promoters and other functional elements using chromosome conformation capture combined with high-throughput sequencing (4C-seq). Our approach can be multiplexed and routinely integrated with other functional genomics assays to facilitate physical characterization of gene regulation.