Smaller Aerobic Granules Significantly Reduce N2O Production by Ammonia-Oxidizing Bacteria: Evidences from Biochemical and Isotopic Analyses.

The mitigation of nitrous oxide (N2O) is of primary significance to offset carbon footprints in aerobic granular sludge (AGS) systems. However, a significant knowledge gap still exists regarding the N2O production mechanism and its pathway contribution. To address this issue, the impact of varying granule sizes, dissolved oxygen (DO), and nitrite (NO2-) levels on N2O production by ammonia-oxidizing bacteria (AOB) during nitrification in AGS systems was comprehensively investigated. Biochemical and isotopic experiments revealed that increasing DO or decreasing NO2- levels reduced N2O emission factors (by 13.8 or 19.5%) and production rates (by 0.08 or 0.35 mg/g VSS/h) via weakening the role of the AOB denitrification pathway since increasing DO competed for more electrons required for AOB denitrification. Smaller granules (0.5 mm) preferred to diminish N2O production via enhancing the role of NH2OH pathway (i.e., 59.4-100% in the absence of NO2-), while larger granules (2.0 mm) induced conspicuously higher N2O production via the AOB denitrification pathway (approximately 100% at higher NO2- levels). Nitrifying AGS systems with a unified size of 0.5 mm achieved 42% N2O footprint reduction compared with the system with mixed sizes (0.5-2.0 mm) under optimal conditions (DO = 3.0 mg-O2/L and NO2- = 0 mg-N/L).

Promoting oxygen vacancies utility for tetracycline degradation via peroxymonosulfate activation by reduced Mg-doped Co3O4: Kinetics and key role of electron transfer pathway.

Developing cobalt-based catalysts with a high abundance of oxygen vacancies (Vo) and exceptional Vo utility efficiency for the prompt removal of stubborn contaminants through peroxymonosulfate (PMS) activation poses a significant challenge. Herein, we reported the synthesis of the reduced Mg-doped Co3O4 nanosheets, i.e. Mg-doped Co3O4-r, via Mg doping and followed by NaBH4 reduction, aiming to degrade tetracycline (TC). Various characterization results illustrated that NaBH4 reduction imparted higher Vo utility efficiency to Mg-doped Co3O4-r, along with an ample presence of reduced Co2+ species and an increased surface area, thereby substantially elevating PMS activation capability. Notably, Mg-doped Co3O4-r achieved more than 97.9% degradation of 20 mg/L TC within 10 min, showing an over 8-fold increase in reaction rate relative to the Mg-doped Co3O4 (kobs: 0.3285 min-1 vs 0.0399 min-1). The high removal efficiency of TC was sustained across a broad pH range of 3-11, even in the presence of common anions and humic acid. Radical quenching trials, EPR outcomes, and electrochemical analysis indicated that neither radicals nor 1O2 were the primary active species. Instead, electron transfer pathway played a dominant role in TC degradation. The Mg-doped Co3O4-r displayed excellent recyclability and versatility. Even after the fifth cycle, it maintained an impressive 83.0% removal of TC. Furthermore, it exhibited rapid degradation capabilities for various pollutants, including levofloxacin, pefloxacin, ciprofloxacin, malachite green, and rhodamine B. The TC degradation pathway was proposed based on LC-MS determination of its degradation intermediates. This study showcases an innovative strategy for the rational design of an efficient cobalt-based activator, leveraging electron transfer pathways through PMS activation to degrade antibiotics effectively.

Investigation of pollutants accumulation in the submerged zone for pyrite-based bioretention facilities under continuous rainfall events.

Submerged zone in bioretention facilities for stormwater treatment has been approved to be an effective structure amendment to improve denitrification capability. However, the role and influence of water quality changes in the submerged zone under natural continuous random rainfall patterns are still not clear, especially when the rainfall is less than the pore water in the submerged zone. In this study, continuous rainfall events with different rainfall volume (light rain-light rain-heavy rain) were designed in a lab-scale woodchip mulched pyrite bioretention facility to test the effects of rainfall pattern. The results exhibited that light rain events significantly affected the pollutant removal performance of bioretention for the next rainfall. Different effects were observed during the long-term operation. In the 5th month, light rain reduced the ammonia removal efficiency of subsequent rainstorm events by 8.70%, while in the 12th month, when nitrate leakage occurred, light rain led to a 40.24% reduction in the next heavy rain event's nitrate removal efficiency. Additionally, light rain would also affect the concentration of by-products in the next rainfall. Following a light rain, the concentration of sulfate in the subsequent light rainfall can increase by 24.4 mg/L, and by 11.92 mg/L in a heavy rain. The water quality in the submerged zone and media characteristics analysis suggested that nitrogen conversion capacity of the substrate and microbes, such as Nitrospira (2.86%) and Thiobacillus (35.71%), as well as the in-situ accumulation of pollutants under light rain played important roles. This study clarifies the relationship between successive rainfall events and provides a more comprehensive understanding of bioretention facilities. This is beneficial for field study of bioretention facilities in the face of complex rainfall events.

Interface engineering-induced perovskite/spinel LaCoO3/Co3O4 heterostructured nanocomposites for efficient peroxymonosulfate activation to degrade levofloxacin.

Conventional perovskite oxides (ABO3) tend to suffer from their inactive surfaces and limited active sites that reduce their catalytic activity and stability, while interface engineering is a facile modulating technique to boost the catalyst's inherent activity by constructing heterogeneous interfaces. In this study, perovskite/spinel LaCoO3/Co3O4 nanocomposites with heterogeneous interfaces were synthesized via sol-gel and in-situ gradient etching methods to activate peroxymonosulfate (PMS) for degrading levofloxacin (LEV). LaCoO3 on the surface was etched into spinel Co3O4, and LaCoO3/Co3O4 nanocomposites with two crystal structures of perovskite and spinel were successfully formed. The surface-modified LaCoO3/Co3O4 exhibited superior catalytic performance with a reaction rate constant more than 2 times that of the original LaCoO3, as well as excellent pH adaptability (3-11) and reusability (more than 6 recyclings) for LEV degradation. Besides, multiple characterization techniques were carried out to find that LaCoO3/Co3O4 possessed a larger specific surface area and richer oxygen vacancies after surface modification, which provided more active sites and accelerated mass transfer rate. The mechanism of reactive oxygen species involved in the reaction system was proposed that LaCoO3/Co3O4 not only reacted with PMS directly to produce SO4•- and •OH but also its surface hydroxyl group helped to form the [≡Co(Ⅲ)OOSO3]+ reactive complex with PMS to produce O2•- and 1O2. In addition, electrochemical experiments demonstrated that the surface electronic structure of LaCoO3/Co3O4 was effectively regulated, exhibiting a faster electron transfer rate and facilitating the redox process. By detecting and identifying degradation intermediates, three degradation pathways for LEV were proposed. Our work provided profound insights into the design of efficient and long-lasting catalysts for advanced oxidation processes.

Pretreatment of septic tank wastewater by packed anaerobic baffled reactor: Pollutant degradation and microbial community succession in different compartments.

Anaerobic baffled reactor (ABR) is widely used in rural sewage treatment due to its unique structure, strong impact load resistance, and low energy consumption. However, there is a lack of research on pollutant degradation patterns and microbial community succession patterns in each compartment of ABR. In this study, a packed anaerobic baffled reactor (PABR) was constructed. The effects of T and HRT on the pollutant removal performance of PABR were investigated, and the pollutant degradation and microbial community succession in different compartments of PABR were studied. The results show that the removal rates of COD, NH4+-N, and TN of PABR can reach 85.54 ± 1.08%, 16.94 ± 1.01%, and 5.64 ± 1.18% respectively, and PABR has a good pollutant removal effect. With the extension of HRT, the COD removal rate of PABR increases steadily, and the NH4+-N and TN removal rate of PABR increases to a certain extent. The recommended HRT is 72 h. T has a significant impact on the COD removal effect of PABR. The increase of T in a certain range is conducive to the removal of pollutants by PABR. The COD removal rate of PABR decreases gradually along the flow direction, and the removal of organic matter is mainly concentrated in the first compartment. PABR has good removal capacity for CODss and better nitrogen removal capacity compared with traditional ABR. The richness and diversity of the microbial community in PABR increased gradually along the flow direction. The bacterial species in each compartment were similar but the proportion was different, showing the characteristics of multi-stage and separated phase operation. This study provides a new reference for the application of ABR in rural sewage treatment.

Insights into the transport and bio-degradation of dissolved inorganic nitrogen in the biochar-pyrite amended stormwater biofilter using dynamic modeling.

The stormwater biofilter is a prevailing green infrastructure for urban stormwater management, but it is less effective in dissolved nitrogen removal, especially for nitrate. The mechanism that governs the nitrate leaching and performance stability of stormwater biofilters is poorly understood. In this study, a water quality model was developed to predict the ammonium and nitrate dynamics in a biochar-pyrite amended stormwater biofilter. The transport of dissolved nitrogen species was described by advection-dispersion models. The kinetics of adsorption and pyrite-based autotrophic denitrification are included in the model and simulated with a steady-state saturated flow. The model was calibrated and validated using eleven storm events. The modeling results reveal that the contribution of pyrite-based autotrophic denitrification to nitrate leaching alleviation improves with the increased drying duration. The nitrate removal efficiency was affected by a series of design parameters. Pyrite filling rate has a minor effect on nitrate removal promotion. Service area ratio and submerged zone depth are the key parameters to prevent nitrate leaching, as they influence the emergence and discharge time of nitrate breakthrough. The high inflow volume (high service area ratio) and small submerged zone can lead to earlier and increased discharge of peak nitrate otherwise the peak nitrate could be retained in the submerged zone and denitrified during the drying period. The developed mechanistic model provides a useful tool to evaluate the treatment ability of stormwater biofilters under varying conditions and offers a guideline for biofilter design optimization.

Assessing Intermediate Formation and Electron Competition during Thiosulfate-Driven Denitrification: An Experimental and Modeling Study.

There is increasing interest in thiosulfate-driven denitrification for low C/N wastewater treatment, but the denitrification performance varies with the thiosulfate oxidation pathways. Models have been developed to predict the products of denitrification, but few consider thiosulfate reduction to elemental sulfur (S0), an undesirable reaction that can intensify electron competition with denitrifying enzymes. In this study, the model using indirect coupling of electrons (ICE) was developed to predict S0 formation and electron competition during thiosulfate-driven denitrification. Kinetic data were obtained from sulfur-oxidizing bacteria (SOB) dominated by the branched pathway and were used to calibrate and validate the model. Electron competition was investigated under different operating conditions. Modeling results reveal that electrons produced in the first step of thiosulfate oxidation typically prioritize thiosulfate reduction, then nitrate reduction, and finally nitrite reduction. However, the electron consumption rate for S0 formation decreases sharply with the decline of thiosulfate concentration. Thus, a continuous feeding strategy was effective in alleviating the competition between thiosulfate reduction and denitrifying enzymes. Electron competition leads to nitrite accumulation, which could be a reliable substrate for anammox. The model was further evaluated with anammox integration. Results suggested that the branched pathway and continuous supply of thiosulfate are favorable to create a symbiotic relationship between SOB and anammox.

A Bayesian-SWMM coupled stochastic model developed to reconstruct the complete profile of an unknown discharging incidence in sewer networks.

Unknown illicit discharges from manufactories often contain toxic chemical matters that are detrimental to the receiving waterbody by deteriorating the performance of wastewater treatment plants. Numerical models that identify these sources and reconstruct the discharging profiles are highly desired for environment management purpose. In this study, a stochastic source identification model that couples Bayesian inference with SWMM is developed to reconstruct the profile of an instantaneous dumpling incidence in sewer networks. The unknown source parameters include location, dumping rate and time of the dumping incidence. Key factors that impact the convergence and performance of the model including walking step size, numbers of unknown source parameters and numbers of monitoring sites are investigated. Results show that the Bayesian-SWMM coupled model is effective and accurate in identifying the unknown sources parameters in an instantaneous dumping event. It is also found that walking step size is crucial for the results to converge to true solutions. Furthermore, it shows that the identified results are highly dependent on the numbers of unknown source parameters. More unknowns result to unsatisfying results. However, the study shows that this limitation could be significantly reduced by using more monitoring site data. One contribution of the study is that errors from measurements and numerical simulation are considered in the identification while results are presented in probabilities with all possible values revealed. This feature is highly practical and efficient when it comes to assist further field screening efforts to pinpoint the true sources.

Aged landfill leachate enhances anaerobic digestion of waste activated sludge.

Anaerobic digestion (AD) is considered as a sustainable pathway to recover energy from organic wastes, but the digestive efficiency for waste activated sludge (WAS) is not as expected due to the limitations in WAS hydrolysis. This study proposes an effective strategy to simultaneously treat WAS and landfill leachate, aiming to promote WAS hydrolysis and enhance organics converting to methane. The effects of landfill leachate on the four stages (i.e., solubilization, hydrolysis, acidogenesis, and methanogenesis) of AD of WAS, as well as the effect mechanisms were investigated. Results showed that adding appropriate amounts of landfill leachate could promote the steps of solubilization, hydrolysis and acidogenesis of WAS, but had no-effect on methanogenesis. The hydrolysis and acidogenesis efficiency in the leachate added digesters were 2.0%-8.4% and 35.2%-72.7% higher than the control digester. Mechanism studies indicated that humic acid (HA) contained in the leachate was conducive to the processes of both hydrolysis and acidogenesis, but detrimental to the methanogenesis. Effects of heavy metals (HMs) on AD of WAS was also dose-dependent. Digestive performance was inhibited by excessive HMs but promoted by moderate dosages. Humic acid and metal ions tend to interact to form complexes, and thus relieve their each inhibition effects. It is also found that the stability of sludge flocs was reduced by the leachate through reducing both apparent activation energy (AAE) and median particle size (MPS) of the sludge. Microbial community and diversity results revealed that the relative abundance of microbes responsible for hydrolysis and acidogenesis increased when landfill leachate was present. This research provides a more technically and economically feasible approach to co-treating and co-utilizing WAS and landfill leachate.

ZIF-mediated N-doped hollow porous carbon as a high performance adsorbent for tetracycline removal from water with wide pH range.

The N-doped porous carbon (NPC) and N-doped hollow porous carbons (NHPC-1 and NHPC-2) were fabricated using ZIF-8 and its composites (resorcinol and formaldehyde coated ZIF-8, ZIF-8@RF and tannic acid coated ZIF-8, ZIF-8@TA) as precursors via high-temperature pyrolysis and their applications for removing tetracycline (TC) from water were investigated. The various technologies, including SEM, TEM, FT-IR, Raman, N2 adsorption-desorption, XRD and XPS were used to characterize the morphology, textual property, phase and microstructure of three porous carbon materials. The adsorption isotherms and kinetics of TC on three porous carbon materials were fitted well with Langmuir model and pseudo-2nd order model, respectively. In terms of the Langmuir model, the maximum TC adsorption capacities on the NPC, NHPC-1 and NHPC-2 were 180.2, 284.9 and 518.1 mg g-1 at 25 °C, respectively. The excellent performance of NHPC-2 for TC removal is mainly attributed to the suitable pore size distribution and pore volume, high nitrogen contents and large amounts of defects. High TC adsorption was achieved in 3-10 pH range and hardly affected by humic acid. TC adsorption on NHPC-2 is spontaneous and endothermic process. The NHPC-2 kept excellent TC adsorption capacity even after eight cycles, showing its good repeatability. Our result indicates that the MOF-mediated N-doped hollow porous carbon is promising for the TC removal from aqueous media.

Enhancement of Organic Matter Removal in an Integrated Biofilm-Membrane Bioreactor Treating High-Salinity Wastewater.

High salinity can strongly inhibit microbial activity and decrease the sedimentation ability of activated sludge. The combination of biofilm and membrane bioreactor is a practical approach towards effective removal of pollutants and low fouling rate. An integrated biofilm-membrane bioreactor (BMBR) treating mustard tuber wastewater was investigated. An average COD removal efficiency of 94.81% and ammonium removal efficiency of 96.84% were achieved at an organic load of 0.5 kg COD/(m3·d). However, the reactor showed a relatively low efficiency in total nitrogen and soluble phosphorus removal due to the lack of anaerobic environment. The increase of influent organic load resulted in a performance degradation because a balance between the degradation ability and pollution has been reached. Images of scanning electron microscopy revealed that halophilic bacteria were the dominant microbe in the system that leads to a loose sludge structure and declined settling properties. It was found that membrane fouling was the consequence of the interaction of microbial activities and NaCl crystallization.

Influence of organic loading rate on integrated bioreactor treating hypersaline mustard wastewater.

Mustard tuber wastewater is characterized by high salinity and high organic content that is potentially detrimental to the biological treatment system and affects the treatment efficiency accordingly. The experiment used the integrated bioreactor to reduce much of the organics in mustard tuber wastewater, and found the influence of organic loading rate on effluent chemical oxygen demand (COD) and phosphate (PO4 (3-) -P). Results showed that under the condition of 10-15 °C, 6 mg/L of dissolved oxygen, the reduction value of COD removal rate in anaerobic and aerobic area was 14.5% and 31.7% when the organic loading rate increased from 2.0 to 4.0 kg COD/m(3) /day. Therefore, an integrated bioreactor should take 2.0 kg COD/m(3) /day organic loading rate in mustard wastewater treatment if the effluent is expected to meet the third level of "Integrated Wastewater Discharge Standard" (GB 8978-1996).

Effects of volumetric load in an anaerobic sequencing batch biofilm treating industrial saline wastewater.

Mustard tuber wastewater is of high salinity ([Cl(-1)]=18∼23 g L(-1)), high organic content (chemical oxygen demand, COD=4000±100 L(-1)) and biodegradability (BOD5/COD≈0.5). The anaerobic sequencing batch biofilm reactor (ASBBR) pre-treatment, an important step to meet national discharge standard, was applied to reduce much of the organics in mustard tuber wastewater. The experiment for the effect of the volumetric load on ASBBR treating mustard tuber wastewater was conducted at different hydraulic retention times (HRTs) and volumetric exchange ratios (λ). The ASBBR operating at 50% biomass density, 30 °C, influent COD concentration of 4000±100 mg L(-1) and pH value of 7.0±0.2, the effluent COD concentration increased from 0.22 to 4 kgCOD m(-3) d(-1) when the volumetric load increased from 100 to 1520 mg L(-1). The effluent COD concentration differed when adopted different HRT and λ under the same volumetric load. And given certain influent levels, a higher performance of ASBBR could be achieved at a lower value of HRT and λ. The optimal operational load could be determined by limiting the COD concentration under different discharge conditions, based on the results obtained in experiments.

Removal, accumulation, and micro-ecosystem impacts of typical POPs in bioretention systems with different media: A runoff infiltration study.

Bioretention systems prove effective in purifying common persistent organic pollutants (POPs) found in urban rainfall runoff. However, the response process of the microecosystem in the media becomes unclear when POPs accumulate in bioretention systems. In this study, we constructed bioretention systems and conducted simulated rainfall tests to elucidate the evolution of micro-ecosystems within the media under typical POPs pollution. The results showed all POPs in runoff were effectively removed by surface adsorption in different media, with load reduction rates of >85 % for PCBs and OCPs and > 80 % for PAHs. Bioretention soil media (BSM) + water treatment residuals (WTR) media exhibited greater stability in response to POPs contamination compared to BSM and pure soil (PS) media. POPs contamination significantly impacted the microecology of the media, reducing the number of microbial species by >52.6 % and reducing diversity by >27.6 % at the peak of their accumulation. Enzyme activities were significantly inhibited, with reductions ranging from 44.42 % to 60.33 %. Meanwhile, in terms of ecological functions, the metabolism of exogenous carbon sources significantly increased (p < 0.05), while nitrogen and sulfur cycling processes were suppressed. Microbial diversity and enzyme activities showed some recovery during the dissipation of POPs but did not reach the level observed before the experiment. Dominant bacterial species and abundance changed significantly during the experiment. Proteobacteria were suppressed, but remained the dominant phylum (all relative abundances >41 %). Bacteroidota, Firmicutes, and Actinobacteria adapted well to the contamination. Pseudomonas, a typical POPs-degrading bacterium, displayed a positive correlation between its relative abundance and POPs levels (mean > 10 %). Additionally, POPs and media properties, including TN and pH, are crucial factors that collectively shape the microbial community. This study provides new insights into the impacts of POPs contamination on the microbial community of the media, which can improve media design and operation efficiency.

Solid-liquid redistribution and degradation of antibiotics during hydrothermal treatment of sewage sludge: Interaction between biopolymers and antibiotics.

Waste activated sludge serves an important reservoir for antibiotics within wastewater treatment plants, and understanding the occurrence and evolution of antibiotics during sludge treatment is crucial to mitigate the potential risks of subsequent resource utilization of sludge. This study explores the degradation and transformation mechanisms of three typical antibiotics, oxytetracycline (OTC), ofloxacin (OFL), and azithromycin (AZI) during sludge hydrothermal treatment (HT), and investigates the influence of biopolymers transformation on the fate of these antibiotics. The findings indicate that HT induces a shift of antibiotics from solid-phase adsorption to liquid-phase dissolution in the initial temperature range of 25-90 °C, underscoring this phase's critical role in preparing antibiotics for subsequent degradation phases. Proteins (PN) and humic acids emerge as crucial for antibiotic binding, facilitating their redistribution within sludge. Specifically, the binding capacity sequence of biopolymers to antibiotics is as follows: OFL>OTC>AZI, highlighting that OFL-biopolymers display stronger electrostatic attraction, more available adsorption sites, and more stable binding strength. Furthermore, antibiotic degradation mainly occurs above 90 °C, with AZI being the most temperature-sensitive, degrading 92.97% at 180 °C, followed by OTC (91.26%) and OFL (52.51%). Concurrently, the degradation products of biopolymers compete for active sites to form novel amino acid-antibiotic conjugates, which inhibits the further degradation of antibiotics. These findings illuminate the effects of biopolymers evolution on intricate dynamics of antibiotics fate in sludge HT and are helpful to optimize the sludge HT process for effective antibiotics abatement.

Multi-media interaction improves the efficiency and stability of the bioretention system for stormwater runoff treatment.

Bioretention systems are one of the most widely used stormwater control measures for urban runoff treatment. However, stable and effective dissolved nutrient treatment by bioretention systems is often challenged by complicated stormwater conditions. In this study, pyrite-only (PO), pyrite-biochar (PB), pyrite-woodchip (PW), and pyrite-woodchip-biochar mixed (M) bioretention systems were established to study the feasibility of improving both stability and efficiency in bioretention system via multi-media interaction. PB, PW, and M all showed enhanced dissolved nitrogen and/or phosphorus removal compared to PO, with M demonstrating the highest efficiency and stability under different antecedent drying durations (ADD), pollutant levels, and prolonged precipitation depth. The total dissolved nitrogen and dissolved phosphorus removal in M ranged between 64%-86% and 80%-95%, respectively, with limited organic matter and iron leaching. Pore water, microbial community, and material analysis collectively indicate that pyrite, woodchip, and biochar synergistically facilitated multiple nutrient treatment processes and protected each other against by-product leaching. Pyrite-woodchip interaction greatly increased nitrate removal by facilitating mixotrophic denitrification, while biochar further enhanced ammonium adsorption and expanded the denitrification area. The Fe3+ generated by pyrite aerobic oxidation was adsorbed on the biochar surface and potentially formed a Fe-biochar composite layer, which not only reduced Fe3+-induced pyrite excessive oxidation but also potentially increased organic matter adsorption. Fe (oxyhydr)oxides intermediate product formed by pyrite oxidation, in return, controlled the phosphorus and organic matter leaching from biochar and woodchip. Overall, this study demonstrates that multi-media interaction may enable bioretention systems to achieve stable and effective urban runoff treatment.

Insights into simultaneous nitrogen and phosphorus removal in biofilm: The overlooked comammox Nitrospira and the positive role of glycogen-accumulating organisms.

Simultaneous nitrogen and phosphorus removal (SNPR) biofilm system is an effective wastewater treatment process. However, the understanding on the mechanism of functional microorganisms driving SNPR is still limited, especially the role of complete ammonia oxidation (comammox) Nitrospira and glycogen-accumulating organisms (GAO). In this study, a sequencing batch biofilm reactor (SBBR) performing SNPR was operated for 249 d. Based on the 16S rRNA gene, comammox amoA amplicon sequencing, metagenomics and batch experiment, we found that comammox Nitrospira was the main ammonia-oxidizing microorganisms (AOM) and provided nitrite for anaerobic ammonia oxidation (anammox) bacteria (AnAOB). Besides, GAO was dominated by the bacteria of genus Defluviicoccus and played a primary role in reducing nitrate rather than nitrite. Fluorescent in situ hybridization (FISH) analysis confirmed that Nitrospira was enriched in the inner layer of the biofilm. Thus, we put forward a novel insight into the mechanism of SNPR biofilm system. Comammox Nitrospira was responsible for nitrite and nitrate production in the inner biofilm, and AnAOB consumed the produced nitrite during the anammox process. While GAO reduced nitrate to nitrite and polyphosphate-accumulating organisms (PAO) converted nitrite to dinitrogen via denitrifying phosphorus removal in the outer biofilm. These findings provide a new understanding in SNPR biofilm system.

Microbial community dynamics and metagenomics reveal the potential role of unconventional functional microorganisms in nitrogen and phosphorus removal biofilm system.

The conventional functional microorganisms for nitrogen and phosphorus removal, such as Nitrosomonas, Nitrobacter, Nitrospira and Candidatus Accumulibacter, were hotspots in past research. However, the role of diverse unconventional functional microorganisms was neglected. In this study, a biofilm system was developed to explore the potential role of unconventional functional microorganisms in nutrients removal. According to the results of microbial community dynamics and metagenomics, complete ammonia oxidizing (comammox) bacteria was 20 times more abundant than ammonia-oxidizing bacteria (AOB) at day 121 and its abundance of amoA gene was almost the same as AOB. Although Nitrospira dominated the nitrite-oxidizing bacteria (NOB), diverse unconventional nxrB-containing microorganisms, particularly Chloroflexi, also significantly contributed to the nitrite oxidation. Binning analysis showed that Myxococcota-affiliated Haliangium had the necessary genes owns by phosphorus-accumulating organisms (PAO) and was likely to be the primary PAO since its abundance (6.38 %) was much higher than other conventional PAO (0.70 %). Comparing metagenome-assembled genomes of comammox bacteria with AOB and ammonia-oxidizing archaea (AOA), it possessed potential metabolic versatility in hydrogen and phosphorus, which may be the primary reason for the positive effect of the alternating anaerobic and aerobic conditions on the enrichment of comammox bacteria. Collectively, our findings broaden the understanding on the microbial mechanism of nitrogen and phosphorus removal in biofilm system.

Nitrite improved nitrification efficiency and enriched ammonia-oxidizing archaea and bacteria in the simultaneous nitrification and denitrification process.

Simultaneous nitrification and denitrification (SND) is effective and energy-saving for wastewater treatment. As an inevitable intermediate product in the SND process, nitrite affects the efficiency of ammonia oxidation and the composition of nitrifiers. To investigate the impact of nitrite on ammonia oxidation efficiency, two reactors performing SND were respectively operated without nitrite (R1 as control) and with 20 mg N/L nitrite addition (R2 as experimental). The total nitrogen removal efficiency was 74.5% in R1 while 99.0% in R2. With nitrite addition (i.e., 20 mg N/L), the ammonia removal rate in R2 increased to 4.5 times of that in R1. The ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) contributed to respective around 46.9% and 41.8% ammonia removal in R2 based on the results of experiments with specific inhibitors. The number of respective AOA and AOB ammonia monooxygenase gene (amoA) copies increased by 280 and 30 times due to nitrite addition, according to the qPCR results. The high-throughput sequencing results illustrated the increase of dominant AOB species from 0.40% in R1 to 1.59% in R2 and the phylogenetic tree analysis revealed a close link to Nitrosospira multiformis. These results indicated that the ammonia removal efficiency was improved and AOA/AOB were enriched by nitrite addition. The specific nitrite reductases in AOA and AOB boosted the adaptation of nitrite addition. This study demonstrated the positive impacts of nitrite addition on the ammonia removal efficiency and rate in the SND process.

Efficiency influence of exogenous betaine on anaerobic sequencing batch biofilm reactor treating high salinity mustard tuber wastewater.

When treating a composite mustard tuber wastewater with high concentrations of salt (about 20 g Cl(-) L(-1)) and organics (about 8000 mg L(-1) COD) by an anaerobic sequencing batch biofilm reactor (ASBBR) in winter, both high salinity and low temperature will inhibit the activity of anaerobic microorganisms and lead to low treatment efficiency. To solve this problem, betaine was added to the influent to improve the activity of the anaerobic sludge, and an experimental study was carried to investigate the influence of betaine on treating high salinity mustard tuber wastewater by the ASBBR. The results show that, when using anaerobic acclimated sludge in the ASBBR, and controlling biofilm density at 50% and water temperature at 8-12 degrees C, the treatment efficiency of the reactor could be improved by adding the betaine at different concentrations. The efficiency reached the highest when the optimal dosage ofbetaine was 0.5 mmol L(-1). The average effluent COD, after stable acclimation, was 4461 mg L(-1). Relative to ASBBR without adding betaine, the activity of the sludge increased significantly. Meanwhile, the dehydrogenase activity of anaerobic microorganisms and the COD removal efficiency were increased by 18.6% and 18.1%, respectively.