Assessing changes in some facial signs of fatigue in Chinese women, induced by a single working day.

BACKGROUND: The impacts of physical fatigue upon some facial signs, induced by a whole day work, have been previously described on Caucasian women. This study aimed at assessing those possibly experienced by Chinese working women under comparable conditions. MATERIAL AND METHODS: Standard photographs of 60 Chinese women working in the same company (aged 20-40 years) were taken at three occasions during their working day (just before, at 4 and 8 h). Focusing on facial signs allowed a panel of experts to grade their respective severities, helped by a referential atlas dedicated to Asian skin. A naïve panel, comprising 64 Chinese women (aged 20-60 years) graded, through an analogic 0-10 scale, the global facial aspect of each subject, particularly its tired-look and dullness and estimated the age of each subject (perceived age). Paired-comparisons in blind and random modes (T0/T4, T4/T8, T0/T8) allowed this naive panel to assess the subjects with a younger/older look, a dullest aspect. RESULTS: A daily work induces, in Chinese women, changes in some facial signs (Nasolabial, Periorbital and Inter-ocular wrinkles, Cheek skin pores). The latter appear more marked between T0 and T4 than between T4 and T8. As compared to those observed among Caucasian women, these changes, although subtle, are of a higher amplitude and concern different facial signs. The naïve panel allowed to describe an increase in the tired look and dullness along the day, more so among the older group (31-40 years), together with an increased perceived age. Tired look, dullness and perceived age appear strongly linked. CONCLUSION: Despite some possible differences in working conditions, some facial signs of Chinese women seem more affected by a fatigue induced by a working day in urban environment, than those of Caucasian women in similar conditions.

CONTEXTE: Les impacts de la fatigue physique, induite par une journée de travail, sur quelques signes faciaux ont été étudiés chez des femmes Caucasiennes. L'objectif de la présente étude visait à déterminer ceux éprouvés par des femmes Chinoises dans des conditions comparables. MATÉRIEL ET MÉTHODES: Des photographies standardisées de 60 femmes Chinoises (20-40 ans), travaillant dans la même entreprise, ont été prises durant leur journée de travail, à trois occasions (matin avant, 4 heures et 8 heures après). Ces photos ont permis de focaliser sur certains signes faciaux dont les sévérités ont alors été scorées, selon un Atlas référentiel de la peau Asiatique. Un panel naïf de 64 femmes Chinoises (20-60 ans) a utilisé une échelle analogique (0-10) pour évaluer l'aspect général du visage, particulièrement sur son aspect fatigué, l'aspect terne de son teint ou donner un âge apparent à chaque sujet. Les comparaisons des photographies par tests par paires, en aveugle et al.éatoire, (T0/T4, T4/T8, T0/T8) demandaient au panel naïf de déterminer quelle photographie montrait un aspect plus jeune ou plus âgé ou un teint plus ou moins terne. RÉSULTATS: Une journée de travail induit chez des femmes Chinoises des modifications de certains signes faciaux (Sillon nasogénien, rides inter-oculaires et péri-orbitales, pores visibles de la joue). Ces derniers apparaissent plus marqués entre T0 et T4 que T4 et T8. Comparés à ceux observés chez des femmes Caucasiennes, ces modifications, bien que subtiles, sont de plus grande ampleur et concernent des signes différents. Le panel naïf a décrit une augmentation de l'aspect fatigué et terne du teint le long de la journée, plus marqués encore chez les femmes les plus âgées (31-45 ans). De plus, teint terne, aspect fatigué et âge apparent ont été trouvés étroitement liés. CONCLUSION: En dépit de quelques possibles différentes conditions de travail, certains signes faciaux des femmes Chinoises semblent plus affectés par une fatigue induite par une journée de travail en milieu urbain que des femmes Caucasiennes dans des conditions comparables.

A severe chronic outdoor urban pollution alters some facial aging signs in Chinese women. A tale of two cities.

BACKGROUND: The impact of a chronic outdoor urban pollution on skin aging-related facial signs is a poorly referenced topic. OBJECTIVE: To assess, through visual grading and referential photographic atlas, possible differences in some facial signs between Chinese women, of comparable ages, living in two close cities very differently exposed to urban pollution. METHODS: The faces of two cohorts of 204 Chinese women (2 × 102) of same age-groups (25-45 years), living in Baoding (a highly polluted city) and Dalian (a less polluted city), for at least 15 years, have been analyzed. Standardized facial photographs (full face and lateral views) were taken and examined, focusing on 26 signs that belong to 3 different clusters (wrinkles and skin texture, pigmentation disorders, and skin pores/skin redness). The severity of each facial sign was graded by 15 trained experts with the help of an Asian skin referential photographic Atlas that illustrates various scales of clinical severity (0-5, 0-8…). In addition, a naïve panel of 80 Chinese non-expert women was asked, while viewing all photographs, to answer to 4 questions regarding skin radiance, dullness, healthy appearance, and perceived age. RESULTS: The increased severity of almost all facial signs was mostly observed in the older group (40-45 years) living in Baoding. In particular, the clinical severity of eight facial signs (five related to skin structure and three related to pigmentation) was found strongly and significantly enhanced by a regular exposure to a severe chronic urban pollution. The naïve panel judged the facial appearance of women from Baoding less radiant, duller, less healthy, and found them older than their Dalian counterparts. The monthly-recorded weather conditions (UV radiance, temperature, wind, precipitations) of the two cities (of same latitude) show that these only differ by their very different Air Quality Indexes (AQI). The daily life conditions of all women (UV exposure, use of cosmetic facial products, intake of sweet or spicy food…) were found similar in all age-groups. CONCLUSION: As compared to a moderately polluted region, a severe chronic outdoor urban pollution favors the aggravation of some facial signs in Chinese women. In short, a highly polluted environment can be viewed as an additional accelerating factor of the skin aging process, when compared to a moderately polluted area.

Seasonally-induced alterations of some facial signs in Caucasian women and their changes induced by a daily application of a photo-protective product.

OBJECTIVES: These were two-fold: (i) to assess the possible changes in some facial signs induced in a 6-month period by the periodical shift from winter to summer in Caucasian women and (ii) to appraise the preventive effects of a strong photo-protective product. METHODS: The facial signs of two cohorts of French women (N= 40 and 42), of comparable ages were graded between winter to summer. One group was left unprotected whereas the other daily applied a strong photo-protective product for 6 months. Facial signs (structural and pigmentation-related) were graded in blind by a panel of 12 experts from photographs taken under standard conditions. A global and focused analysis of the skin colour or dark spots, when present, was carried out through spectro-radiometry under diffuse and standardized visible light, using the L*, a*, b* referential system. RESULTS: The unprotected group showed significant changes in summer as compared to winter on 10 facial signs (two-third of the studied signs) that presented an increased severity, of variable respective amplitude. Five signs among the 10 were particularly and significantly affected by the seasonal transition, of an amplitude above the precision of the grading scale. Three of these five signs concerned structural elements (wrinkles), the two others being related to vascular disorders (redness). These season-induced alterations appear efficiently reduced in the photo-protected group. The colour of the facial skin then appears more homogeneous, less red, less dull, all criteria being quantified by the L*, a*, b* referential system. The comparison with a previous work carried out on Chinese women, through a similar protocol, shows that the photo-protective product brings, in Caucasian women, a more important effect upon structural and vascular features than upon pigmentation disorders, inversely to the results previously observed in Chinese women. CONCLUSION: The alterations in some facial signs occurring in a 6-month period between winter and summer are confirmed in Caucasian women, mostly related to structural (wrinkles) and vascular elements. Such changes appear alleviated or prevented by daily applications of a strong sun photo-protective product.

Natural extracellular matrix scaffolds recycled from human salivary digests: a morphometric study.

OBJECTIVE: A challenge in engineering tissues is to supply parenchymal cells with suitable scaffolds which ideally reproduce the extracellular matrix (ECM). This study tested the hypothesis of preserving the 'residual connective tissue' remaining after mechanical and enzymatic release of cells from human submandibular gland biopsies (that we named 'natural ExtraCellular Matrix scaffolds', nECMsc) to be used as recycled natural scaffolds. The objective was to test whether nECMsc and native salivary tissue were comparable morphologically, in ECM proteins composition, and in cell seeding efficiency. METHODS: Following cell isolation procedures, nECMsc were kept, either fresh or frozen (sectioned into 12-µm-thick slices), and examined with high-resolution electron microscopy (HRSEM) for its three-dimensional structure, and with picrosirius red staining and immunogold staining for ECM protein composition and distribution, respectively. nECMsc were seeded with human epithelial cells and fibroblasts to assess cell attachment and proliferation in short-term experiments. RESULTS: Under HRSEM, nECMsc had comparable fiber arrangement to original glands. Histochemical and immunogold-labeling examinations revealed the presence of collagen types I, III, and IV. Seeded epithelial cells and fibroblasts attached, proliferated (14-55%), and were alive (86-99%) after 4-8 days of culture. CONCLUSIONS: nECMsc retained native ECM proteins and maintained their distribution. Seeded cells remained viable on nECMsc.

Genomic organization of a human 5beta-reductase and its pseudogene and substrate selectivity of the expressed enzyme.

The enzyme 5beta-reductase catalyzes the reduction of the 4-ene of 3-ketosteroids, converting them into 5beta-dihydro-3-ketosteroids and, thus, could be involved in the metabolism of 4-cholestene-3-one, progesterone, 17~-hydroxyprogesterone, aldosterone, corticosterone, cortisol, 4-androstenedione, and testosterone. In this study, we report the genomic structure of a human 5beta-reductase gene, its tissue distribution, the characterization of an intronless pseudogene and the substrate selectivity of the enzyme. The gene coding for the active 5beta-reductase contains nine exons like most members of the aldo-keto reductase family, but the sequence covered by the gene, more than 42 kb, is much longer than the sequence of other members of this family. There are many large introns, especially introns 3, 4 and 7 that span approx. 7 kb, and intron 1 that contains more than 10 kb. Northern blot analysis showed three band sizes of 1.3, 2.2 and 2.7 kb. The 1.3 and 2.7 kb bands are highly expressed in the liver while weaker 2.2 and 1.3 kb bands have been observed in the testis and colon, respectively. We also identified an intronless gene having 86% homology with the 5beta-reductase cDNA sequence. Since its sequence contains many stop codons, this gene is most probably a pseudogene. To determine more precisely the substrate selectivity of the enzyme, we established a stable cell line expressing human 5beta-reductase in transformed embryonic kidney (HEK-293) cells. The transfected cells efficiently catalyze the transformation of progesterone, androstenedione, 17alpha-hydroxyprogesterone and testosterone. However, they catalyze much less efficiently the transformation of compounds containing an 11beta-hydroxy group, such as aldosterone, corticosterone and cortisol. In addition to its role in cholesterol catabolism, it is well recognized that 5beta-reductase inactivates active androgens. Indeed, 5beta-dihydrotestosterone (5beta-DHT), the product of the reduction of testosterone by 5beta-reductase, is not active while its 5~-isomer (DHT) is the most potent natural androgen. Recent findings show that 5beta-pregnanes are active ligands in the induction of CYP3A through the orphan receptor hPAR. Our results thus open an opportunity for studying the new role of 5beta-reductase in the formation of a new type of active steroids.

Structure and activity of the murine type 5 17beta-hydroxysteroid dehydrogenase gene(1).

17beta-Hydroxysteroid dehydrogenases (17beta-HSDs) play a crucial role in the control of active sex steroid intracellular levels. Seven types of 17beta-HSD have been described. In this study, we report the cloning and characterization of the mouse type 5 17beta-HSD belonging to the aldo-keto reductase superfamily, in contrast with types 1, 2, 3, 4, 6, and 7 17beta-HSD which belong to the short-chain alcohol dehydrogenase family. The gene spans 16 kb and contains 9 exons separated by 8 introns. Primer extension analysis identified a major transcription start site beginning 50 nucleotides upstream from the ATG initiation codon. Northern blot analysis showed a high mRNA expression level in the liver and a weaker signal in the kidney. To determine more precisely the substrate specificity of the enzyme, we established a stable cell line expressing mouse type 5 17beta-HSD in transformed human embryonic kidney (293) cells. The transfected cell line preferentially catalyzes the transformation of 4-androstenedione (4-dione) and androstanedione (A-dione) into testosterone (T) and dihydrotestosterone (DHT), respectively. This data is somewhat in contradiction with a previous study that described the enzyme as estradiol 17beta-dehydrogenase. Our results indicate that the rate of transformation of estradiol (E(2)) to estrone (E(1)) represents only 1% of the rate of transformation of 4-dione to T. Mouse type 5 17beta-HSD shares 76% amino acid sequence identity with human type 5 17beta-HSD; 71%, 76%, 76% with rat 3alpha-HSD and human types 1 and 3 3alpha-HSDs, respectively; and 71%, 69% and 77% with mouse, rat and human 20alpha-HSD, respectively.

[Biomechanical study and digital modeling of traction resistance in posterior thoracic implants]. / Etude biomécanique et modélisation numérique de la résistance à la traction des implants thoraciques postérieurs.

PURPOSE OF THE STUDY: The purpose of this experimental study was to compare posterior fixation systems using hooks and screws implanted in the thoracic spine. This study was completed by a digital analysis using the finished element method. MATERIAL AND METHODS: For the experimental study, we used 7 human thoracic spines. Forty-nine groups of 2 vertebrae were individualized. Traction was applied to maximum breaking force measured on an Instron. We used two types of instrumentations, alternating 4 pedicle screws and 2 pedicle-lamina hooks. For the digital study, we used a vertebral model composed of nearly 63 000 nodes and 14 000 elements. Elastic field calculations were carried out with a finished element abacus. RESULTS: The base of the pedicles broke when traction was applied to a pedicle-lamina hook assembly. The medial part of the pedicle broke when traction was applied to a pedicle screw assembly. Maximul break strength for hooks was 1 108 +/- 510 N. It was 820 +/- 418 N for 4 mm diameter screws and 1 395 +/- 435 for 5 mm screws. The most fragile vertebrae were T5-T6 and T7-T8. the screw-instrumented model showed that stress concentrated on the medial aspect of the pedicle, inside the medullary canal. Using a long screw did not reduce the stress force significantly. The hook-instrumented model showed that stress was greatest on the lower part of the pedicle. DISCUSSION: From a mechanical point of view, screw instrumentation is the more appropriate type of fixation. Screw fixation did not however demonstrate its superiority during the traction tests. For 4 mm screws, resistance was 23% weaker than with a hook assembly and for 5 mm screws, it was only 12% stronger. Pullout may be attributed to two principal causes, either fracture of the bony anchoring of the screw system or breakage of the pedicle. Bone thread pullout occurs when the screw threads do not penetrate sufficiently deep into the cortical bone due to the small diameter of the screw shaft. Using a larger diameter screw raises however the problem of damaging the pedicle. Pedicle breakage is seen with stronger stress forces and constitutes the upper limit of maximum break force. This leads us to formulate the hypothesis that in most cases, screw pullout occurs by breakage of the bony threading. Screws are less effective if they are not properly anchored in the pedicles, probably the reason for their relative weakness. Screw diameter should be adapted to the size of each pedicle. This would allow better transmission of stress from the screw to the pedicle. Hooks apply further stress to the vertebrae. The digital study showed that using a long screw crossing through the vertebra does not reduce the stress applied to the pedicles enough to justify its use.

Evidence of hepatic glucagon resistance associated with hepatic steatosis: reversal effect of training.

The present study was undertaken to test the hypothesis that a high-fat diet-induced hepatic steatosis is associated with a reduction in hepatic glucose output (HGO) in response to a hyperglucagonemic infusion, and that this postulated state of hepatic glucagon resistance in high-fat fed rats is attenuated by concurrent exercise training. In four groups of anesthetized rats, glucagon (2 ug/kg/min iv) was infused over a period of 60 min to measure HGO. Two groups of rats were either fed a standard (SD) or a high-fat (HF; 42 % kcal) diet for eight weeks and were assigned either to a Sedentary (Sed) or a treadmill-trained (TR) group. Training was initiated two weeks after the beginning of the diet protocol and was progressively increased over a period of 6 weeks reaching 60 min at 26 m/min, 10 % grade, for the last 3 weeks. The HF compared to the SD diet resulted in approximately 28 % higher (p < 0.01) liver triglyceride levels in Sed rats. This increase was completely prevented by the exercise training program in the HF-TR group. Plasma glucagon ( approximately 90,000 pg/ml) and insulin ( approximately 500 pmol/l) levels were increased to a similar extent in all four groups, with the exception of higher (p<0.05) insulin levels in SD-Sed group. Glucagon induced-hyperglycemia ( approximately 300 mg/dl) was higher (p<0.05) in the SD-Sed than in HF-Sed and SD-TR groups. Glucagon infusion resulted in a significantly (p<0.05) lower increase ( approximately 35 %) in HGO in HF-Sed compared to SD-Sed group. The lower level of HGO in HF-Sed compared to SD-Sed rats was observed whether HGO was measured after 25, 40, or 60 min of glucagon infusion. Exercise training in HF fed rats resulted in a significant (p<0.05) attenuation (50 %) of the state of HF-induced glucagon resistance. Comparisons of all individual liver triglyceride and 60-min HGO values revealed that liver triglyceride values were highly (p<0.001) predictive of the decreased glucagon action on HGO (R= -0.849). The present results indicate that the feeding of a high-fat diet induces a state of hepatic glucagon resistance, which is partially attenuated by concurrent exercise training. It is suggested that liver lipid infiltration may interfere with the action of glucagon, thus inducing glucagon resistance in liver.

Do people with HIV/AIDS disclose their HIV-positivity to dentists?

This study documents behaviours of people with HIV/AIDS regarding the disclosure of their HIV-positivity when seeking dental care. An anonymous survey was conducted in Québec, Canada, from 1993 to 1995, using a sample of 463 people with HIV/AIDS recruited from different sources. Over 80% of respondents reported having sought dental care since becoming aware of their positivity. Of these, 54% reported having always disclosed their HIV-positivity to dentists, while 25% reported never having disclosed this information to dentists. However, 83% of all respondents preferred that the dentist be aware of their HIV status. Respondents gave reasons related to the sociopathological impact of HIV infection to explain their behaviours. The predictors of disclosure of HIV-positivity to the dentist were: gender, main source of payment for dental care, prior disclosure of HIV status to family members, prior disclosure of HIV status to co-workers, and trust in the maintenance of confidentiality by the dentist. These results emphasize the need to use universal precautions in the dental office and to promote dentist-patient relations free of discrimination, so that people with HIV/AIDS are not reluctant to disclose their health status and are confident that such disclosure will lead to care best adapted to their condition.

[Electrical dental anesthesia (EDA), an alternative to local anesthesia?]. / L'anesthésie dentaire électrique (ADE), une alternative aux anesthésiques locaux?

Electrical dental anesthesia has recently been suggested as an alternative to local anesthetics for the control of pain related to certain dental acts. This article updates the evidence which supports the efficiency, indications and contra-indications of such a procedure. Although this technique has certain advantages, its use is limited by: 1) how cooperative the patient may be; 2) the time required to explain the procedure to the patient and that which is required to produce the needed clinical effect; and, 3) how efficient it may be relative to the patient in question and the dental treatment rendered.

Cellular localization of estrogen receptor beta messenger ribonucleic acid in cynomolgus monkey reproductive organs.

There is now evidence that the recently identified estrogen receptor (ER) beta is more widely distributed in the body than is ER-alpha. In order to gain more information about the role of ER-beta in reproduction, we have investigated by in situ hybridization the localization of mRNA expression of this ER subtype in adult monkey reproductive organs. In the pituitary gland of animals of both sexes, in both the anterior and intermediate lobes, a large number of cells were positive. No specific signal was observed in the posterior lobe. In the ovary, granulosa cells in primary and growing follicles highly expressed ER-beta mRNA. The theca interna cells were also strongly labeled. In some corpora lutea, the luteal cells were strongly labeled, while in other ones, the signal was weak. A hybridization signal was also detected in the ovarian surface epithelium. In the uterus, ER-beta mRNA was found in high concentration in glandular epithelial cells and stromal cells of the endometrium, while weaker labeling was consistently observed in smooth muscle cells. In the mammary gland, labeling was detected in the epithelial cells of acini and interlobular ducts as well as stromal cells. In the testis, specific labeling was detected in the seminiferous epithelium whereas the interstitial Leydig cells were unlabeled. Although it was not possible to clearly identify all the positive cell types, it appears that Sertoli cells as well as the vast majority of germinal cells express ER-beta mRNA. In the prostate, the secretory epithelial cells exhibited a specific autoradiographic reaction while the stromal cells did not show mRNA expression. The epithelial cells of the prostatic urethra showed a strong labeling. No hybridization signal was detected in the seminal vesicles. It then appears quite clear that ER-beta is expressed in a cell-specific manner in all the monkey reproductive organs studied. In the female, the wide distribution of these receptors in the ovary and uterus suggests that ER-beta may play an important role in the mediation of the known effects of estrogen in reproduction functions. In the male testis and prostate, ER-beta has been found in cells that contain very little or no ER-alpha. The role of circulating or locally produced estrogens in the male reproductive system remains to be clarified.

Effect of angiotensin II on calcium reabsorption by the luminal membranes of the nephron.

In the rat and the rabbit, a number of studies have reported the effects of angiotensin II (ANG II) on Na(+) reabsorption by the proximal (PT) and distal (DT) convoluted tubules of the kidney. The aim of the present study was to examine the effect of ANG II on Ca(2+) uptake by the luminal membranes of the PT and DT of the rabbit. Incubation of PT and DT with 10(-12) M ANG II enhanced the initial Ca(2+) uptake in the two segments. Dose-response experiments revealed, for Ca(2+) as well as for Na(+) transport, a biphasic action with a maximal effect at 10(-12) M. Ca(2+) transport by the DT luminal membrane presents a dual kinetic. ANG II action influenced the high-affinity Ca(2+) channel, increasing maximal velocity from 0.72 +/- 0.03 to 0.90 +/- 0.05 pmol x microg(-1) x 10 s(-1) (P < 0.05, n = 3) and leaving the Michaelis-Menten constant unchanged. The effect of ANG II was abolished by losartan, suggesting that the hormone is acting through AT1 receptors. In the PT, calphostin C inhibited the effect of the hormone. It is therefore probable that protein kinase C is involved as a messenger. In the DT, however, neither Rp cAMP, calphostin C, nor econazole (a phospholipase A inhibitor) influenced the hormone action. Therefore, the mechanisms involved in the hormone action remain undetermined. Finally, we questioned whether ANG II acts in the same DT segment as does parathyroid hormone on Ca(2+) transport. The two hormones increased Ca(2+) transport, but their actions were not additive, suggesting that they both influence the same channels in the same segment of the distal nephron, i.e., the segment responsible for the high-affinity calcium channel.

Community periodontal index of treatment needs and prevalence of periodontal conditions.

BACKGROUND/AIMS: In 1977, the World Health Organization (WHO) proposed a new index, the community periodontal index of treatment needs (CPITN) to evaluate the periodontal treatment needs of populations. The aim of this study is to compare different approaches of recording and presenting the CPITN. METHODS: A sample of 2110 subjects aged 35-44 years were examined between September 1994 and July 1995, throughout the province of Quebec, Canada. For each tooth (3rd molars excluded), the presence of bleeding and calculus, the level of epithelial attachment, and the depth of periodontal pockets were measured. Periodontal pocket depths were measured from the edge of the free gingiva, at 2 sites (mesiovestibular and vestibular), as well as all around the tooth. RESULTS: Only 8.5% of adults had at least one tooth with a 6 mm or deeper periodontal pocket when probing on 2 sites, whereas if probing is done all around the tooth, this percentage is 2.5x higher (21.4%). The partial recording of pocket depths (10 index teeth recommended by WHO, or 2 quadrants chosen at random) resulted in an underestimation of the prevalence of subjects with at least one tooth with a periodontal pocket (CPITN score 3 and 4). Among subjects with at least one tooth with a 6 mm or deeper periodontal pocket, 12% were not detected with the 10 index teeth recording, and 25% go undetected with the measure on 2 quadrants. Finally, using the % of subjects with periodontal pockets overestimates the prevalence of deep pockets compared with using sextants. Indeed, close to 30.0% of sextants have no treatment needs, whereas only 5.2% of subjects are in this category. Similarly, 7.7% of sextants have at least one tooth with a 6 mm or deeper periodontal pocket, yet there are 3x more subjects in this category (21.4%).

Effects of introducing physical training in the course of a 16-week high-fat diet regimen on hepatic steatosis, adipose tissue fat accumulation, and plasma lipid profile.

OBJECTIVE: We recently reported that an 8-week high-fat diet-induced hepatic steatosis was completely prevented if an exercise training programme was introduced and pursued concurrently with the diet. The purpose of the present study was to determine the extent to which introducing exercise training at mid-point in the course of a 16-week high-fat diet regimen contributes to the reversal of liver lipid infiltration and the reduction of blood lipid profile deterioration and body fat accumulation. DESIGN AND SUBJECTS: Two groups of rats were fed a high-fat diet (42% kcal) for 16 weeks, one remaining sedentary during this entire period (HF-Sed) and the other being exercise trained for the last 8 weeks (HF-Tr). A third group was fed a standard diet and remained sedentary for all 16 weeks (SD-Sed). Training (5 days/week for 8 weeks) began 8 weeks after introducing the high-fat diet and consisted of treadmill running that was progressively increased to reach 60 min at 26 m/min, 10% grade, for the last 4 weeks. MEASUREMENTS: Various parameters including liver lipid infiltration, fat depots and blood lipids. RESULTS: Unexpectedly, liver lipid infiltration was not significantly higher in HF-Sed than in SD-Sed rats (means+/-s.e.: 14.9+/-1.7 vs 12.3+/-0.4 mg/g; P>0.05). High-fat compared to age-matched standard fed rats also showed an absence of difference (P>0.05) in the weight of total visceral fat pads (13%), plasma nonesterified fatty acids (NEFA), and leptin concentrations, but depicted significantly (P<0.01) higher values for subcutaneous fat pad weight and plasma triacyglycerol. Exercise training largely decreased visceral and subcutaneous fat accumulation by 30 and 26%, respectively (P<0.01) as well as NEFA, triacylglycerol, and leptin concentrations (P<0.01). CONCLUSION: Liver lipid infiltration does not seem to progress linearly over 16 weeks of high-fat feeding in light of what has previously been observed after 8 weeks of high-fat feeding. Introducing a training programme in the course of a 16-week high-fat diet protocol reduced adiposity, plasma NEFA, and leptin concentrations below the levels observed in standard fed rats. These data indicate that, exercise training, whether conducted concurrently or introduced during the course of a high-fat diet, is an asset to reduce the deleterious effects of a high-fat diet.