Early predictors of childhood caries among 4-year-old children: a population-based study in north-eastern France.

BACKGROUND: Untreated early childhood caries (ECC) can trigger a number of negative consequences, including pain, chewing difficulties, insufficient physical development and low academic performance. Therefore, ECC impacts the oral health-related to quality of life. That is why it is important to assess the ECC prevalence and to determine the associated risk factors. AIM: The aim of this study was to investigate the relationship between early predictor factors and dental caries among 4-year-old French children, considering the socioeconomic factors, dietary and oral hygiene practices, the access and the follow-up by paediatric dentist. DESIGN: The study was a cross-sectional observation. A random sample of 4-year-old children was selected among 596 Moselle's public nursery schools in north-eastern France. Data were gathered from clinical dental examinations and a structured questionnaire completed by parents. RESULTS: In total, 425 subjects with completed questionnaires and clinical examinations were included. The prevalence of ECC and S-ECC (Severe-ECC), including only cavitated lesions, d3mft, were 15.8% and 5.9%, respectively. Multivariable logistic regression modelling identified four main factors associated with a high risk of ECC that were sweet intake (adjusted odds ratio ORadjusted = 3.43, 95% CI = 1.57-7.53), brushing habits (ORadjusted = 2.25, 95% CI = 1.23-4.21), childcare arrangement (ORadjusted = 2.27, 95% CI = 1.23-4.21) and maternal educational level (ORadjusted = 1.87, 95% CI = 1.06-3.31). These four factors have a cumulative effect. Only 4% of children presented teeth restoration. CONCLUSIONS: This study highlighted the impact of the family environment on the oral health status of the preschool children. The identification of the risk factors should allow to strengthen preventive measures and initiate oral health education program for children and their family.

Social representations of mandatory vaccination in patients and general practitioners in Moselle, France.

Vaccine controversies affect the image of vaccination. At the end of 2014, there was a shortage of tetra- and pentavalent vaccines, although immunization obligation was being discussed. Our objective in this context was to study the social representations of mandatory vaccination in general practitioners and patients. Thirty general practitioners and 30 patients were recruited for this study based on hierarchal evocation. Each provided seven associations, which were revealed spontaneously in response to the evocation of the inductive concept: "mandatory vaccination". An order of importance and a connotation were attributed to them. These associations were classified into subthemes and themes. For each, the frequency of occurrence, the median rank of importance, and the polarity index were calculated and then compared. The image of vaccination was positive (polarity index from doctors and patients: +0.22 and +0.24, respectively). The doctors mentioned first the organization of the vaccine follow-up (21.9%). For patients, the benefits of vaccination predominated (22.38%), although these benefits were considered more important by the doctors. Doctors raised vaccine controversies more frequently than patients and were more concerned with the practical aspects of vaccination (injection, pain). The current controversies have not eroded the representations of mandatory vaccination. However, doctors are critical towards the media and vaccination policy. Patients, on the other hand, are afraid of side effects and do not have complete confidence in vaccination. Immunization obligation does not appear to be challenged by either of the two populations. To sustain immunization, our main asset remains communication and information to patients.

Relationship between two oxidative stress biomarkers, malondialdehyde and 8-oxo-7,8-dihydro-2'-deoxyguanosine, in the freshwater bivalve Unio tumidus.

This study addresses the question of the relation between cellular and genomic oxidative damages in freshwater bivalves in realistic conditions of exposure in the field. Membrane and genomic oxidative damages were studied by means of lipid peroxidation and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo), respectively. Malondialdehyde (MDA) was used as an indicator of lipid peroxidation. The biomarkers were measured in the digestive gland and the gills of mussels (Unio tumidus) after 7 and 21 days of exposure in four ponds of the Moselle Basin, during three field studies conducted in 1999 and 2000. Effects measured at three sites (1R, 3C and 4M) were moderate and lipid peroxidation was slightly enhanced in mussels transferred in these ponds. In contrast, an important degradation was observed at site 2V reflected by a sharp increase in MDA and 8-oxodGuo concentrations in 2000. The biomarker responses agreed with the results of physicochemical analyses that indicated a worsening of water quality at the same site. Globally, a relationship was found between MDA increase and 8-oxodGuo formation, in the digestive gland after 7 days of exposure, and later in the gills (21 days). Responses of the digestive glands and gills to the oxidative parameters appeared correlated only after 21 days of exposure. The biomarkers selected confirmed their sensitivity for appraising the water quality of hydrosystems.

DNA damage (comet assay) and 8-oxodGuo (HPLC-EC) in relation to oxidative stress in the freshwater bivalve Unio tumidus.

The relationships between DNA damage and oxidative stress in the digestive gland, gills and haemocytes of the freshwater bivalve Unio tumidus were investigated. Two markers of genotoxicity were measured: DNA breaks by means of the comet assay, and oxidative DNA lesions by means of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) measured using high-performance liquid chromatography (HPLC) coupled to electrochemical detection. Lipid peroxidation was evaluated by measuring malondialdehyde (MDA) tissue levels. Effects were studied after exposure of bivalves for 6 days to benzo[a]pyrene (B[a]P) (50 and 100 microg l(-1)) and ferric iron (20 and 40 mg l(-1)), applied alone or in combination. Lipid peroxidation in the digestive gland and gills resulted from exposure to Fe3+ or B[a]P whatever the concentrations tested. DNA oxidatively formed lesions were induced in the two tissues at a higher level after B[a]P exposure than after Fe3+ treatment. No significant dose-response relationship was found with the two compounds and no synergistic effect was observed between Fe3+ and B[a]P. The gills appeared less sensitive than the digestive gland to DNA lesions expressed as 8-oxodGuo and comet results. Good correlations were noted between 8-oxodGuo and comet. MDA and DNA damage did not correlate as well, although it was stronger in the digestive gland than in the gills. Production of mucus by the gills likely served to prevent lesions by reducing the bioavailability of the chemicals tested, which could explain that dose-effect relationships and synergistic effects were not observed.