Temporal dynamics and genomic programming of plasma cell fates.

Affinity-matured plasma cells (PCs) of varying lifespans are generated through a germinal center (GC) response. The developmental dynamics and genomic programs of antigen-specific PC precursors remain to be elucidated. Here, using a model antigen in mice, we demonstrate biphasic generation of PC precursors, with those generating long-lived bone marrow PCs preferentially produced in the late phase of GC response. Clonal tracing using single-cell RNA sequencing and B cell antigen receptor sequencing in spleen and bone marrow compartments, coupled with adoptive transfer experiments, reveals a new PC transition state that gives rise to functionally competent PC precursors. The latter undergo clonal expansion, dependent on inducible expression of TIGIT. We propose a model for the proliferation and programming of precursors of long-lived PCs, based on extended antigen encounters in the GC.

Drought stimulus enhanced stress tolerance in winter wheat (Triticum aestivum L.) by improving physiological characteristics, growth, and water productivity.

The impact of drought events on the growth and yield of wheat plants has been extensively reported; however, limited information is available on the changes in physiological characteristics and their effects on the growth and water productivity of wheat after repeated drought stimuli. Moreover, whether appropriate drought stimulus can improve stress resistance in plants by improving physiological traits remains to be explored. Thus, in this study, a pot experiment was conducted to investigate the effects of intermittent and persistent mild [65%-75% soil water-holding capacity (SWHC)], moderate (55%-65% SWHC), and severe drought (45%-55% SWHC) stress on the growth, physiological characteristics, yield, and water-use efficiency (WUE) of winter wheat. After the second stress stimulus, persistent severe drought stress resulted in 30.98%, 234.62%, 53.80%, and 31.00% reduction in leaf relative water content, leaf water potential, photosynthetic rate (Pn), and indole-3-acetic acid content (IAA), respectively, compared to the control plants. However, abscisic acid content, antioxidant enzyme activities, and osmoregulatory substance contents increased significantly under drought stress, especially under persistent drought stress. After the second rehydration stimulus (ASRR), the actual and maximum efficiency of PSII and leaf water status in the plants exposed to intermittent moderate drought (IS2) stress were restored to the control levels, resulting in Pn being 102.56% of the control values; instantaneous WUE of the plants exposed to persistent severe drought stress was 1.79 times that of the control plants. In addition, the activities of superoxide dismutase, peroxidase, catalase, and glutathione reductase, as well as the content of proline, under persistent mild drought stress increased by 52.98%, 33.47%, 51.95%, 52.35%, and 17.07% at ASRR, respectively, compared to the control plants, which provided continuous antioxidant protection to wheat plants. This was also demonstrated by the lower H2O2 and MDA contents after rehydration. At ASRR, the IAA content in the IS2 and persistent moderate drought treatments increased by 36.23% and 19.61%, respectively, compared to the control plants, which favored increased aboveground dry mass and plant height. Compared to the control plants, IS2 significantly increased wheat yield, WUE for grain yield, and WUE for biomass, by 10.15%, 32.94%, and 33.16%, respectively. Collectively, IS2 increased grain growth, yield, and WUE, which could be mainly attributed to improved physiological characteristics after drought-stimulated rehydration.

Greenhouse gas emissions and drivers of the global warming potential of vineyards under different irrigation and fertilizer management practices.

In the context of global warming and low water and fertilizer utilization efficiency in vineyards, identifying the driving factors of global warming potential (GWP) and proper irrigation and fertilization management strategies are crucial for high grape yields and emission reduction. In this experiment, drip fertigation technology was used, including three irrigation levels (W3 (100% M, where M is the irrigation quota), W2 (75% M) and W1 (50% M)) and four fertilization levels (F3 (648 kg hm-2), F2 (486 kg hm-2), F1 (324 kg hm-2) and F0 (0 kg hm-2)). Traditional furrow irrigation and fertilization (CG) and rainfed (CK) treatments were used as control treatments. The results indicated that under the drip fertigation system, fertilization significantly increased the grape leaf chlorophyll relative content (SPAD) and leaf area index (LAI) within a fertilizer application of 0-486 kg hm-2. Irrigation primarily had a direct positive effect on the water-filled pore space (WFPS) in the 0-60 cm soil layer, and the residual soil nutrient content was mainly affected by fertilization. The vital stage for reducing greenhouse gas emissions was the fruit-inflating and fruit-rendering stages. The CG treatment not only failed to ensure high grape yield but also adversely affected the soil environment and the reduction of greenhouse gas emissions in the vineyard. Fertilization had a direct positive effect on the grape SPAD, LAI, yield, and soil residual nutrient content. GWP was primarily directly driven by SPAD, WFPS, and soil residual nutrient content, while grape yield was primarily directly driven by fertilization and SPAD. In conclusion, the W2F2 treatment (25 % reduced irrigation and 486 kg hm-2 of fertilization) of drip fertigation in the vineyard was the preferred irrigation and fertilizer management strategy for maintaining good vine vigor and balancing grape yield and environmental benefits.

Antibody Fc-receptor FcεR1γ stabilizes cell surface receptors in group 3 innate lymphoid cells and promotes anti-infection immunity.

Group 3 innate lymphoid cells (ILC3) are crucial for maintaining mucosal homeostasis and regulating inflammatory diseases, but the molecular mechanisms governing their phenotype and function are not fully understood. Here, we show that ILC3s highly express Fcer1g gene, which encodes the antibody Fc-receptor common gamma chain, FcεR1γ. Genetic perturbation of FcεR1γ leads to the absence of critical cell membrane receptors NKp46 and CD16 in ILC3s. Alanine scanning mutagenesis identifies two residues in FcεR1γ that stabilize its binding partners. FcεR1γ expression in ILC3s is essential for effective protective immunity against bacterial and fungal infections. Mechanistically, FcεR1γ influences the transcriptional state and proinflammatory cytokine production of ILC3s, relying on the CD16-FcεR1γ signaling pathway. In summary, our findings highlight the significance of FcεR1γ as an adapter protein that stabilizes cell membrane partners in ILC3s and promotes anti-infection immunity.