RESUMEN
Objective To observe the effect of Dangua Recipe (DR) on adiponectin receptor 2 (AdipoR2) expression in liver of apolipoprotein E knockout (ApoE⻹â») diabetic mice. Methods Eight- week-old ApoE⻹⻠mice were randomly divided into the model control group, the DR group, the pioglitazone group, and the combined treatment group. Besides, a C57 group was set up consisting of same age C57BL/ 6J mice. Streptozotocin (STZ) was injected to ApoE⻹⻠mice in the four groups to induce diabetic model, and they were intervened by corresponding drugs. After 12 weeks the effect of DF on glucose-lipid metabolism was observed. mRNA and protein expressions of AdipoR2 in liver were detected. Histomorphological changes of the liver were observed by hematoxylineosin ( HE) staining, red Ο fat dyeing, and Masson staining, respectively. Results Contents of fasting blood glucose (FBG) , total cholesterol (TC) , and low-density lipoprotein cholesterol (LDL-C) were reduced more significantly in the DR group than in the model group. DR could promote liver expression of AdipoR2 mRNA and protein expressions in ApoE⻹⻠mice, which was significantly higher than that of the model group (P <0. 01) , and better than that of pioglitazone (P <0. 05, P <0. 01). DR could improve ectopic fat deposition and fibrosis of liver cells in diabet- ic ApoE⻹⻠mice significantly, which was better than that of the pioglitazone group and the combined treatment group. Conclusion DR could significantly improve liver lipid metabolism , and reduce liver fat deposition and fibrosis, which might possibly be associated with promoting AdipoR2 expression in liver.
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Apolipoproteínas E , Diabetes Mellitus Experimental , Medicamentos Herbarios Chinos , Receptores de Adiponectina , Animales , Apolipoproteínas E/genética , Diabetes Mellitus Experimental/metabolismo , Medicamentos Herbarios Chinos/farmacología , Hígado , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Receptores de Adiponectina/efectos de los fármacos , Receptores de Adiponectina/metabolismoRESUMEN
OBJECTIVE: To study the effect of Dangua Recipe (DGR) on glycolipid metabolism, vascular cell adhesion molecule-1 (VCAM-1) and its mRNA expression level of transgenic Apo E(-/-) mouse with spontaneous atherosclerosis, thus revealing its partial mechanism for curing diabetes mellitus (DM) with angiopathy. METHODS: Diabetic model was prepared by peritoneally injecting streptozotocin (STZ) to Apo E(-/-) mouse. Totally 32 modeled mice were stratified by body weight, and then divided into 4 groups referring to blood glucose levels from low to high by random digit table, i.e., the model group (MOD, fed with sterile water, at the daily dose of 15 mL/kg), the DGR group (fed with DGR at the daily dose of 15 mL/kg), the combination group (COM, fed with DGR at the daily dose of 15 mL/kg and pioglitazone at the daily dose of 4.3 mg/kg), and the pioglitazone group (PIO, at the daily dose of 4.3 mg/kg), 8 in each group. Another 8 normal glucose C57 mouse of the same age and strain were recruited as the control group. All interventions lasted for 12 weeks by gastrogavage. The fasting blood glucose (FBG), body weight, food intake, water intake, skin temperature, the length of tail, and the degree of fatty liver were monitored. The hemoglobin A1c (HbA1c), total cholesterol (TC), and LDL-C were determined. Endothelin-1 (ET-1) was determined by radioimmunoassay. Nitrogen monoxidum (NO) was determined by nitrate reductase. The kidney tissue VCAM-1 level was analyzed with ELISA. The expression of VCAM-1 mRNA in the kidney tissue was detected with real time quantitative PCR. RESULTS: Compared with the control group, the body weight and food intake decreased, water intake increased in all the other model groups (P < 0.05). Besides, the curve of blood glucose was higher in all the other model groups than in the control group (P < 0.01). Compared with the model group, the body weight increased; levels of HbAlc, TC, LDL-C, ET-1, and VCAM-1 were significantly lower; and skin temperature was higher in the DGR group (P < 0.05, P < 0.01). Compared with the PIO group, body weight, the increment of body weight, FBG, TC, and LDL-C were lower (P < 0.05, P < 0.01); food intake and water intake increased more and the tail length was longer in the DRG group (P < 0.01). There was no statistical difference in the level of NO among groups. The degree of fatty liver in the model group was significantly severer than that in the control group (P < 0.05). It was obviously alleviated in the DGR group (P < 0.05) when compared with the model group and the PIO group (P < 0.05, P < 0.01). But it was severer in the PIO group than in the model group (P < 0.01). The degree of fatty liver in the combination group ranged between that of the DGR group and the PIO group (P < 0.05). The level of VCAM-1 mRNA expression was significantly lower in the DGR group than in the model group, the PIO group, and the combination group (P < 0.05). CONCLUSIONS: DGR had effect in lowering blood glucose and blood lipids, and fighting against fatty liver of transgenic Apo E(-/-) mouse with spontaneous atherosclerosis. DGR played an effective role in preventing and treating DM with angiopathy by comprehensively regulating glycolipid metabolism and promoting the vascular function.
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Diabetes Mellitus Experimental/tratamiento farmacológico , Medicamentos Herbarios Chinos/farmacología , Molécula 1 de Adhesión Celular Vascular/metabolismo , Animales , Apolipoproteínas E/genética , Glucemia/metabolismo , Diabetes Mellitus Experimental/sangre , Angiopatías Diabéticas/tratamiento farmacológico , Lípidos/sangre , Masculino , Ratones , Ratones Noqueados , Pioglitazona , ARN Mensajero/genética , Distribución Aleatoria , Tiazolidinedionas/farmacología , Molécula 1 de Adhesión Celular Vascular/genéticaRESUMEN
OBJECTIVE: To assess the anti-inflammatory effect of Aike Mixture (AKM) on rats with nonbacterial prostatitis (NBP). METHODS: Rat model of NBP was established by injection of Xiaozhiling Injection. The experimental rats were randomized into 7 groups: the three AKM groups (A1, A2 and A3), treated with high, middle and low dose of AKM respectively, the 2 positive control groups (C1 and C2), treated by Bazheng Mixture (BZM) and Qianliexian Decoction (QLXD) respectively, the model control group (Cm) and the sham-operative control group (Cso). The pathological changes in rats' prostate were observed by light microscope and transmission electron microscope. RESULTS: Significant differences in the number and structure of acini, mesenchyma, as well as the degrees of anti-fibroplasia and inflammatory cell infiltration were shown between the treated groups (A1, A2, A3, C1, C1, C2) and the untreated groups (Cm, Cso), with statistical significance (P < 0.05 or P < 0.01). However, the anti-fibroplasia and anti-inflammatory effects in A1 were better than that in the two positive control groups significantly (P < 0.05 or P < 0.01). CONCLUSIONS: AKM is a new TCM drug functioned for dispersing Gan-qi in treating NBP. It shows a better efficacy than that of BZM and QLXD, the two Chinese herbal medicines for clearing heat with remove dampness and activating blood circulation to remove stasis, respectively. Aike Mixture; prostatitis; pathological observation
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Antiinflamatorios no Esteroideos/uso terapéutico , Medicamentos Herbarios Chinos/uso terapéutico , Fibroblastos/efectos de los fármacos , Fitoterapia , Prostatitis/tratamiento farmacológico , Animales , Fibroblastos/patología , Masculino , Prostatitis/patología , Distribución Aleatoria , Ratas , Ratas Sprague-DawleyRESUMEN
OBJECTIVE: To study the anti-inflammatory and analgesic actions of Aike Mixture (AKM). METHODS: A total of 100 male mice were randomly assigned into 5 groups: a normal control group, a drug control group (a hydrocortisone subgroup and an atropine subgroup), a high-dose AKM group, a mid-dose AKM group and a low-dose AKM group. Xylene was spread on the left ear of the experimental mice to induce inflammation, and 1% acetic acid solution injected into the abdominal cavity to produce pain so as to cause the body bend. Different doses of AKM were given and their actions observed. RESULTS: AKM had obvious anti-inflammatory effect on the xylene-induced ear tumefaction and inhibited the pain-caused body bend in the AKM groups, with significant difference from the normal control (P < 0.01). CONCLUSION: AKM has good anti-inflammatory and analgesic effects, which is of clinical significance in the treatment of chronic prostatitis.
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Ácido Oleanólico/análogos & derivados , Fitoterapia , Prostatitis/tratamiento farmacológico , Saponinas/farmacología , Animales , Enfermedad Crónica , Modelos Animales de Enfermedad , Combinación de Medicamentos , Masculino , Ratones , Ratones Endogámicos ICR , Ácido Oleanólico/farmacología , Ácido Oleanólico/uso terapéutico , Saponinas/uso terapéuticoRESUMEN
OBJECTIVE: To investigate the effect of Dan-gua Fang on adenosine 5'-monophosphate (AMP) activated protein kinase (AMPK) α expression in liver and subsequent improvement of glucose and lipid metabolism. METHODS: Forty 13-week-old diabetic Goto-Kakizaki (GK) rats were randomly divided into model, Dan-gua Fang, metformin and simvastatin groups (n=10 for each), and fed high-fat diet ad libitum. Ten Wistar rats were used as normal group and fed normal diet. After 24 weeks, liver expression of AMPKα mRNA was assessed by real-time PCR. AMPKα and phospho-AMPKα protein expression in liver was evaluated by Western blot. Liver histomorphology was carried out after hematoxylin-eosin staining, and blood glucose (BG), glycosylated hemoglobin A1c (HbA1c), food intake and body weight recorded. RESULTS: Similar AMPKα mRNA levels were found in the Dan-gua Fang group and normal group, slightly higher than the values obtained for the remaining groups (P<0.05). AMPKα protein expression in the Dan-gua Fang group animals was similar to other diabetic rats, whereas phospho-AMPKα (Thr-172) protein levels were markedly higher than in the metformin group and simvastatin group (P<0.05), respectively. However, phosphor-AMPKα/AMPKα ratios were similar in all groups. Dan-gua Fang reduced fasting blood glucose with similar strength to metformin, and was superior in reducing cholesterol, triglycerides, high-density lipoprotein cholesterol as well as improving low-density lipoprotein cholesterol in comparison with simvastatin and metformin. Dan-gua Fang decreases plasma alanine aminotransferase (ALT) significantly. CONCLUSION: Dan-gua Fang, while treating phlegm-stasis, could decrease BG and lipid in type 2 diabetic GK rats fed with high-fat diet, and effectively protect liver histomorphology and function. This may be partly explained by increased AMPK expression in liver. Therefore, Dan-gua Fang might be an ideal drug for comprehensive intervention for glucose and lipid metabolism disorders in type 2 diabetes mellitus.