Sulfate reduction behavior in pressure-bearing leachate saturated zone.

Attention should be paid to the sulfate reduction behavior in a pressure-bearing leachate saturated zone. In this study, within the relative pressure range of 0-0.6 MPa, the ambient temperature with the highest sulfate reduction rate of 50°C was selected to explore the difference in sulfate reduction behavior in a pressure-bearing leachate saturated zone. The results showed that the sulfate reduction rate might further increase with an increase in pressure; however, owing to the effect of pressure increase, the generated hydrogen sulfide (H2S) could not be released on time, thereby decreasing its highest concentration by approximately 85%, and the duration extended to about two times that of the atmospheric pressure. Microbial community structure and functional gene abundance analyses showed that the community distribution of sulfate-reducing bacteria was significantly affected by pressure conditions, and there was a negative correlation between disulfide reductase B (dsrB) gene abundance and H2S release rate. Other sulfate reduction processes that do not require disulfide reductase A (dsrA) and dsrB genes may be the key pathways affecting the sulfate reduction rate in the pressure-bearing leachate saturated zone. This study improves the understanding of sulfate reduction in landfills as well as provides a theoretical basis for the operation and management of landfills.

Source and risk assessment of heavy metals in mining-affected areas in Jiangxi Province, China, based on Monte Carlo simulation.

In recent years, heavy metal contamination of soils has become a major concern in China due to the potential risks involved. To assess environmental pollution and human health risks in a typical heavy metal polluted site in Jiangxi Province, a thorough evaluation of the distribution, pollution levels, and sources of heavy metals in soils of the Yangmeijiang River watershed was conducted in this study. Positive matrix factorization and Monte Carlo simulation were used to evaluate the ecological and human health risks of heavy metals. The research findings indicate that heavy metal pollution was the most severe at the depth of 20-40 cm in soils, with local heavy metal pollution resulting from mining and sewage irrigation. The high-risk area accounted for 91.11% of the total area. However, the pollution level decreased with time due to sampling effects, rainfall, and control measures. Leaf-vegetables and rice were primarily polluted by Cd and Pb. The main four sources of heavy metals in soils were traffic emission, metal smelting, agricultural activities and natural sources, mining extraction, and electroplating industries. Heavy metals with the highest ecological risk and health risk are Cd and As, respectively. The non-carcinogenic and carcinogenic risks of children were 7.0 and 1.7 times higher than those of adults, respectively. Therefore, children are more likely to be influenced by heavy metals compared to adults. The results obtained by the risk assessments may contribute to the identification of specific sources of heavy metals (e.g., traffic emissions, metal smelting, mining excavation, and electroplating industries). Additionally, the environmental impacts and biotoxicity associated with various heavy metals (e.g., Cd and As) can also be reflected. These outcomes may serve as a scientific basis for the pollution monitoring and remediation in the mining-affected areas.

Unravelling microbial drivers of the sulfate-reduction process inside landfill using metagenomics.

Hydrogen sulfide (H2S) is one of the common landfill odor. This research demonstrates that the sulfate transformation behavior is significantly enhanced during the landfill process, accompanied by a shift in microbial structure. The relative abundance of dissimilatory sulfate reduction (DSR) and thiosulfate oxidation by SOX (sulfur-oxidation) complex gradually decreases through the landfill processes while the assimilatory sulfate reduction (ASR) demonstrates the opposite behavior. The major module for landfill sulfate reduction is ASR, accounting for 31.72% ± 2.84% of sulfate metabolism. Based on the functional genes for the sulfate pathway, the drivers for sulfate biotransformation in landfills were determined and further identified their contribution in the sulfate metabolism during landfill processes. Pseudomonas, Methylocaldum, Bacillus, Methylocystis and Hyphomicrobium were the top 5 contributors for ASR pathway, and only one genus Pseudomonas was found for DSR pathway. Among the 26 high-quality metagenome-assembled genomes of sulfate functional species, 24 were considered novel species for sulfuric metabolism. Overall, this study provides unique insight into the sulfate transformation process related to the H2S odor control in landfill management.

Localized intensification of arsenic methylation within landfill leachate-saturated zone.

Leachate-saturated zone (LSZ) of landfills is a complicated biogeochemical hotspot due to the continuous input of electron donors and acceptors from the top refuse layer with leachate migration. In this study, the methylation behavior of the arsenic (As) was investigated. The results indicate that As-methylation processes are influenced by temperature fields in LSZ. The dimethylarsinic acid biotransformation capability can be enhanced with an increase in temperature. Microbial diversity, quantification of functional gene (arsM), and co-occurrence network analysis further characterized the drivers of As methylation in LSZ. As-biogeochemical cycle pathways, as well as As-functional gene distribution among different temperature fields, were modeled on the basis of KEGG annotation. Binning analysis was further employed to assemble As-methylated metagenomes, enabling the identification of novel species for As methylation in landfills. Then, 87 high-quality draft metagenome-assembled genomes (MAGs) were reconstructed from LSZ refuse samples; nearly 15 % (13 of 87) belonged to putative As-methylates functional MAGs. Combined with the model of the As-biogeochemical cycle, nine putative functional species could complete methylation processes alone. The findings of this study highlighted the temperature influence on the As-methylation behavior in LSZ and could facilitate the management of As contamination in landfills.

Sulfate-reduction behavior in waste-leachate transition zones of landfill sites.

The sulfate reduction behavior of the waste-leachate transition zone of landfill was investigated at different temperatures and moisture contents. Marked differences in the sulfate reduction behavior were observed in the waste-leachate transition zone. The highest H2S concentration was observed when the solid-to-liquid ratio was 1:3 at both temperatures. Although more leachate led to higher H2S concentrations, the solid-to-liquid ratio was likely of subordinate significance compared with temperature. The microbial community was more unstable at 50 °C and more extensive mutualistic interactions among bacteria were observed, resulting in SRB showing a more violent response to changes in the solid-to-liquid ratio. At 25 °C, it's the opposite. A temperature of 25 °C was suitable for most SRB (such as Desulfomicrobium and Desulfobulbus), while some specific SRB that did not contain the functional genes (such as Dethiobacter and Anaerolinea) played a pivotal role in the significant differences in sulfate reduction behavior observed at 50 °C. This study provides a theoretical basis for controlling the release of H2S from landfill.

Evolution of sulfate reduction behavior in leachate saturated zones in landfills.

The sulfate reduction behavior of the landfill leachate saturated zone under different temperatures was investigated. The results showed that temperature had significant effects on sulfate reduction behavior. The sulfate reduction efficiency was the highest at high temperatures (55 °C and 45 °C), followed by mesophilic temperature (35 °C). Normal temperature 25 °C was far less effective than 55 °C, 45 °C and 35 °C. High abundances of aprA and dsrA genes were distributed under high temperatures. Through indicator species analysis and functional comparison, some key taxa were identified as putative key genera for sulfate reduction. Under high temperature, Paenibacillus could effectively degrade dimethyl sulfide. DsrAB is present in the genome of Tissierella. Gordonia, Syntrophomonas, and Lysinibacillus under mesophilic temperature indicates the potential of these organisms to degrade heterogenous biomass, environmental pollutants or other natural polymers with slow biodegradation. This microbial function is similar to that of the putative key genera under normal (25 °C) temperature. Most of the putative key genera belong to Firmicutes, Proteobacteria and Myxococcota. This study provides theoretical support for the control of hydrogen sulfide release from landfills.

Sulfate reduction behavior in the leachate saturated zone of landfill sites.

Municipal solid waste landfills are considered one of the most important parts of the sulfur cycle. However, few studies have focused on sulfate reduction in the leachate saturated zone, where the temperature may be variable. In this work, the sulfate reduction behavior was evaluated in a landfill leachate saturated zone under temperatures between 30 and 80 °C. The results show that microbial sulfate reduction is high in the saturated zone, especially when the temperature is at 50-60 °C. The microbial diversity and the abundance of functional genes results reveal that specific sulfate-reducing bacteria such as Dethiobacter, the bacteria that offer energy to them, and genes other than dsrA and dsrB may have a close relationship with the variation in the reduction of sulfate. This work may improve the knowledge of sulfate reduction in the landfill sites and therefore offer theoretical support to management strategies.