RESUMEN
POPULATION: Amerindian populations: Huastecos (n=97), Otomies de la Sierra (n=41), Otomies del Valle (n=40), and Tepehuas (n=13).
Asunto(s)
Cromosomas Humanos Y , Etnicidad/genética , Genética de Población , Haplotipos , Secuencias Repetidas en Tándem , Dermatoglifia del ADN , Frecuencia de los Genes , Humanos , Masculino , México , Reacción en Cadena de la PolimerasaRESUMEN
Two polymorphic sites of the microsomal epoxide hydrolase gene (EPHX1, 113Tyr-->113His, 139His-->139Arg) and four glutathione S-transferase genes (GSTM1, GSTM3, GSTP1, GSTT1) were genotyped in a group of patients with larynx cancer (N=204) and in a group of healthy controls (N=203), all Spanish caucasians. After adjusting for gender, age, and tobacco smoking, none of the polymorphisms alone were found to be associated with larynx cancer risk. The analysis of EPHX1/GST combinations, however, showed a significant over-representation of patients with a combination of 113Tyr/113Tyr EPHX1 and 105Ile/105Ile GSTP1 (adjusted odds ratio (OR): 1.95; 95% confidence interval (CI): 1.02-3.78). The calculation of the predicted epoxide hydrolase (EH) activity also showed an increased risk for the individuals with both predicted high activity EH and 105Ile/105Ile GSTP1 (OR: 2.90; 95% CI: 1.10-7.67). These results on larynx cancer tend to confirm a former study on lung cancer (Cancer Lett. 173 (2001) 155) suggesting the existence of an interaction between variants of EH and GSTpi, both enzymes being involved in the metabolism of aromatic hydrocarbons, that may increase susceptibility to tobacco-related cancers.
Asunto(s)
Carcinoma de Células Escamosas/genética , Epóxido Hidrolasas/genética , Glutatión Transferasa/genética , Neoplasias Laríngeas/genética , Polimorfismo Genético , Adulto , Anciano , Anciano de 80 o más Años , Carcinoma de Células Escamosas/enzimología , Estudios de Casos y Controles , Femenino , Genotipo , Humanos , Isoenzimas/genética , Neoplasias Laríngeas/enzimología , Masculino , Microsomas/enzimología , Persona de Mediana Edad , Reacción en Cadena de la Polimerasa , Factores de RiesgoRESUMEN
Allele and genotype frequencies for the D1S80 (MCT118) locus have been determined in a population sample from Barcelona (Spain) using the polymerase chain reaction (PCR) amplification and nonradioactive detection. In a total of 216 unrelated individuals, 24 alleles (23 common and 1 rare variant) and 67 genotypes (64 common and 3 variants) were observed. The 216 individuals came from 162 blood samples taken for paternity studies, 16 bloodstains from forensic cases, and 38 root hairs from normal individuals. The D1S80 locus demonstrated a heterozygosity of 0.7916, and a power of discrimination of 0.9731. The distribution of genotypes is in agreement with expected values according to the Hardy-Weinberg equilibrium. Additionally, the population from Barcelona differs, significantly, from the Finnish population and also, but with lower differences, from a U.S.A. Caucasian population. © 1993 Wiley-Liss, Inc.