RESUMEN
The aim of this study was to evaluate the possibility of free-ranging animals/hunting dogs as sources of infection in the vaccinia virus (VACV) transmission chain. Serological, cell culture and molecular assays were conducted in 56 free-ranging animals and 22 hunting dogs. ELISA/neutralizing assays showed that two (2.5%) capybaras (Hydrochoerus hydrochaeris) had anti-OPV positive antibodies, while all samples tested negative through PCR/cell culture. After being hit by cars on roads, capybaras that exhibited neither clinical signs nor any association with bovine outbreaks had neutralizing antibodies against the Orthopoxvirus, as detected through plaque-reduction neutralizing tests and ELISA. Evidence exists regarding peridomestic capybaras acting as a source of the virus and serving as a link between wild and urban environments, thus contributing to viral maintenance.
Asunto(s)
Reservorios de Enfermedades/veterinaria , Enfermedades de los Perros/transmisión , Enfermedades de los Roedores/epidemiología , Roedores/virología , Virus Vaccinia/inmunología , Vaccinia/veterinaria , Animales , Animales Salvajes , Anticuerpos Neutralizantes , Brasil/epidemiología , Bovinos , Reservorios de Enfermedades/virología , Enfermedades de los Perros/epidemiología , Enfermedades de los Perros/virología , Perros , Pruebas de Neutralización/veterinaria , Enfermedades de los Roedores/virología , Vaccinia/epidemiología , Vaccinia/transmisión , Vaccinia/virología , Virus Vaccinia/aislamiento & purificación , ZoonosisRESUMEN
The aim of this study is to assess the frequency of rabies antibodies in free-ranging capuchin monkeys (Cebus apella nigritus) in a fragmented, environmentally protected, rural area of southeastern Brazil. Thirty-six free-ranging monkeys were tested by the rapid fluorescent focus inhibition test for detection of antibodies against rabies virus. Four individuals (11.11 %) had neutralizing antibody titers ≥ 0.25 IU/mL, demonstrating rabies virus exposure.