Electrically controlled liquid-crystal microlens matrix with a nested electrode array for efficiently tuning and swinging focus.

A new type of electrically controlled liquid-crystal microlens matrix (EC-LCMM) with a nested electrode array for efficiently tuning and swinging focus, which means that the focus position can be adjusted in three dimensions, is proposed. The EC-LCMM is constructed by a 10 × 10 arrayed annular-sector-shaped aluminum electrode with a central microhole of 140µm diameter and three annular-sectors of 210µm external diameter and the period length of 280µm. To the arrangement of the patterned electrode, both the 10 × 10 LC microlens array based on the annular-sector-shaped aluminum electrode and the 9 × 9 LC microlens array based on an arrayed quasi-quadrilateral-ring-shaped electrode can be obtained. The 9 × 9 LC microlens array is formed by matching adjacent four annular-sector-shaped sub-electrodes in the 10 × 10 LC microlenses. The developed EC-LCMM can be used to electrically tune focus along the optical axis and also swing focus over a focal plane selected. The typical performances include: electrically tunable focusing in a driving voltage range of 3~7Vrms, the focal length in a range of 2~0.6mm, and the maximum focus swing distance being 16µm. For effectively describing the focus swing efficiency, the parameters of SF and SA are defined, which are the ratios between the focus swinging distance and the current focal length along the optical axis, and between the focus swinging extent and the external diameter of a single annular-sector-shaped aluminum electrode, respectively. The SF and SA of the EC-LCMM are ~16‰ and ~7.6%, respectively. It can be expected that the complex wavefront can be more efficiently measured and adjusted according to the EC-LCMM-based Shack-Hartmann wavefront measuring and adjusting architecture.

Optical properties of electrically controlled arc-electrode liquid-crystal microlens array for wavefront measurement and adjustment.

An electrically controlled arc-electrode liquid-crystal microlens array (AE-LCMLA), with tuning and swing focus, is proposed, which can be utilized to replace the traditional mechanically controlled microlenses and also cooperate with photosensitive arrays to solve the problems of measuring and further adjusting a strong distortion wavefront. The top patterned electrode of a single LC microlens is composed of three arc-electrodes distributed symmetrically around a central microhole for constructing the key controlling structures of the LC cavity in the AE-LCMLA. All the arc-electrodes are individually controlled, and then the focal spot of each microlens can be moved freely in a three-dimensional fashion including along the optical axial direction and over the focal plane by simply adjusting the driving signal voltage applied over each arc-electrode, independently. The featured performances of the AE-LCMLA in a wavelength range of ∼501-561 nm are the driving signal voltage being relatively low (less than ∼11 Vrms), the focal length tuning range being from ∼2.54 mm to ∼3.50 mm, the maximum focus swing distance being ∼52.92 µm, and the focus swing ratio K being ∼20‰.

Effect of dietary supplementation with sanguinarine on meat quality and lipid metabolism of broilers.

Dietary Macleaya cordata extract (MCE) can improve the meat quality of poultry. However, the specific mechanism by which MCE regulates the meat quality has not been clarified yet. Sanguinarine (SAN) is one of the important natural active components in MCE. Our study aims to explore the regulatory mechanism of dietary SAN supplementation on meat quality through transcriptomic and gut microbiome analysis, thereby providing a basis for regularing meat quality with MCE. 240 1-day-old broilers were divided into 4 groups according to different doses of SAN (0, 0.225, 0.75, and 2.25 mg/kg). The results indicated that SAN significantly improve the physicochemical quality indicators of breast and thigh muscle in broilers, improved the serum biochemical indexes. Through transcriptome sequencing analysis of the liver and ileum tissues of broilers, we found that the differentially expressed genes induced by SAN were mainly enriched in lipid metabolism, which were related to the peroxisome proliferator-activated receptor (PPAR) pathway. It reconfirmed that SAN can regulate lipid metabolism in the body by promoting the expression of genes related to cholesterol metabolism, fatty acid transport and oxidation by RT-PCR, this ultimately affects the physicochemical quality of muscle. Additionally, through 16S rRNA sequencing analysis, we found that dietary addition of SAN increased the relative abundance of Bacteroides, Lactobacillus and unclassified_f_Lachnospiraceae, while decreased the relative abundance of Alistipes in ceca. To further investigate the impact of gut microbiota on lipid metabolism, we conducted a correlation analysis of PPAR pathway factor expression in cecum tissue and microflora structure. The results showed that Bacteroides exhibited a positive correlation with the expression of most genes in the PPAR signaling pathway. Unclassified_f__Lachnospiraceae is positively correlated with PPARγ, Cytochrome P450 family 7 subfamily A member 1 (CYP7A1) and Acyl-CoA synthetase long-chain family member 5 (ACSL5). In conclusion, dietary addition of SAN can promote the genes expression of the PPAR pathway, target the regulation of intestinal microflora structure and abundance and regulate lipid metabolism, thereby improving meat quality of broilers.

Synergistic Effect of Treatment with Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus and Lipopolysaccharide on the Inflammatory Response of Porcine Pulmonary Microvascular Endothelial Cells.

The highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) often causes secondary bacterial infection in piglets, resulting in inflammatory lung injury and leading to high mortality rates and significant economic losses in the pig industry. Microvascular endothelial cells (MVECs) play a crucial role in the inflammatory response. Previous studies have shown that HP-PRRSV can infect porcine pulmonary MVECs and damage the endothelial glycocalyx. To further understand the role of pulmonary MVECs in the pathogenesis of HP-PRRSV and its secondary bacterial infection, in this study, cultured porcine pulmonary MVECs were stimulated with a HP-PRRSV HN strain and lipopolysaccharide (LPS). The changes in gene expression profiles were analyzed through transcriptome sequencing, and the differentially expressed genes were verified using qRT-PCR, Western blot, and ELISA. Furthermore, the effects on endothelial barrier function and regulation of neutrophil trans-endothelial migration were detected using the Transwell model. HP-PRRSV primarily induced differential expression of numerous genes associated with immune response, including IFIT2, IFIT3, VCAM1, ITGB4, and CCL5, whereas LPS triggered an inflammatory response involving IL6, IL16, CXCL8, CXCL14, and ITGA7. Compared to the individual effect of LPS, when given after HN-induced stimulation, it caused a greater number of changes in inflammatory molecules, such as VCAM1, IL1A, IL6, IL16, IL17D, CCL5, ITGAV, IGTB8, and TNFAIP3A, a more significant reduction in transendothelial electrical resistance, and higher increase in neutrophil transendothelial migration. In summary, these results suggest a synergistic effect of HP-PRRSV and LPS on the inflammatory response of porcine pulmonary MVECs. This study provides insights into the mechanism of severe lung injury caused by secondary bacterial infection following HP-PRRSV infection from the perspective of MVECs, emphasizing the vital role of pulmonary MVECs in HP-PRRSV infection.

Research on a Dual-Mode Infrared Liquid-Crystal Device for Simultaneous Electrically Adjusted Filtering and Zooming.

A new dual-mode liquid-crystal (LC) micro-device constructed by incorporating a Fabry⁻Perot (FP) cavity and an arrayed LC micro-lens for performing simultaneous electrically adjusted filtering and zooming in infrared wavelength range is presented in this paper. The main micro-structure is a micro-cavity consisting of two parallel zinc selenide (ZnSe) substrates that are pre-coated with ~20-nm aluminum (Al) layers which served as their high-reflection films and electrodes. In particular, the top electrode of the device is patterned by 44 × 38 circular micro-holes of 120 µm diameter, which also means a 44 × 38 micro-lens array. The micro-cavity with a typical depth of ~12 µm is fully filled by LC materials. The experimental results show that the spectral component with needed frequency or wavelength can be selected effectively from incident micro-beams, and both the transmission spectrum and the point spread function can be adjusted simultaneously by simply varying the root-mean-square value of the signal voltage applied, so as to demonstrate a closely correlated feature of filtering and zooming. In addition, the maximum transmittance is already up to ~20% according the peak-to-valley value of the spectral transmittance curves, which exhibits nearly twice the increment compared with that of the ordinary LC-FP filtering without micro-lenses.