RESUMEN
Comparison of the genome of the Gram negative human pathogen Haemophilus quentini MP1 with other species of Haemophilus revealed that, although it is more closely related to Haemophilus haemolyticus than Haemophilus influenzae, the pathogen is in fact genetically distinct, a finding confirmed by phylogenetic analysis using the H. influenzae multilocus sequence typing genes. Further comparison with two other H. quentini strains recently identified in Canada revealed that these three genomes are more closely related than any other species of Haemophilus; however, there is still some sequence variation. There was no evidence of acquired antimicrobial resistance within the H. quentini MP1 genome nor any mutations within the DNA gyrase or topoisomerase IV genes known to confer resistance to fluoroquinolones, which has been previously identified in other H. quentini isolates. We hope by presenting the annotation and genetic comparison of the H. quentini MP1 genome it will aid the future molecular detection of this potentially emerging pathogen via the identification of unique genes that differentiate it from other species of Haemophilus.
Asunto(s)
Girasa de ADN/genética , Topoisomerasa de ADN IV/genética , Genoma Bacteriano , Haemophilus influenzae/genética , Haemophilus/genética , Girasa de ADN/metabolismo , Topoisomerasa de ADN IV/metabolismo , Expresión Génica , Variación Genética , Haemophilus/clasificación , Haemophilus/metabolismo , Haemophilus influenzae/clasificación , Haemophilus influenzae/metabolismo , Tipificación de Secuencias Multilocus , Filogenia , Secuenciación Completa del GenomaRESUMEN
Haemophilus quentini is a rare and distinct genospecies of Haemophilus that has been suggested as a cause of neonatal bacteremia and urinary tract infections in men. We present the draft whole-genome sequence of H. quentini MP1 isolated from an infant in the United Kingdom, aiding future identification and detection of this pathogen.