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1.
Chem Biodivers ; 21(8): e202400756, 2024 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-38847466

RESUMEN

This study reports chemical composition, phytotoxic and antibiofilm activities of essential oils (EOs) of R. dasycarpa and R. sphaerocarpa from Morocco. EOs were analyzed by GC/MS and their phytotoxicities were evaluated against germination and seedling growth of Lolium multiflorum, Sinapis alba and Raphanus sativus. The antimicrobial and anti-biofilm activities were studied against Gram-negative (Pseudomonas aeruginosa, Escherichia coli and Acinetobacter baumannii) and Gram-positive bacteria (Staphylococcus aureus and Listeria monocytogenes). Both EOs were abundant in oxygenated monoterpenes (40.01% and 23.57 %, respectively). Carvacrol is the predominant component in R. dasycarpa EO (17.80 %), and it also represents an appreciable amount in R. sphaerocarpa (8.96 %). R. sphaerocarpa showed total inhibition at high doses against all seeds. S. alba seeds were the most sensitive to all EOs. Minimum inhibitory concentration (MIC) values indicated significant inhibition for R. sphaerocarpa, between 24 and 30 µg/mL, with a remarkable antibacterial potential and biofilm formation inhibition. R. sphaerocarpa EO showed significant biofilm inhibition with variable efficacy depending on the strain and concentration, except for S. aureus. R. dasycarpa exhibited activity against all bacterial strains and effect on metabolism with activity also on mature biofilms. Results suggest that Retama EOs could have potential applications in the fields of food and health.


Asunto(s)
Antibacterianos , Biopelículas , Pruebas de Sensibilidad Microbiana , Aceites Volátiles , Biopelículas/efectos de los fármacos , Aceites Volátiles/farmacología , Aceites Volátiles/química , Aceites Volátiles/aislamiento & purificación , Antibacterianos/farmacología , Antibacterianos/química , Antibacterianos/aislamiento & purificación , Marruecos , Bacterias Grampositivas/efectos de los fármacos , Bacterias Gramnegativas/efectos de los fármacos , Germinación/efectos de los fármacos
2.
J Clin Ultrasound ; 52(8): 1087-1095, 2024 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-39082319

RESUMEN

OBJECTIVES: To evaluate the prevalence of chromosomal anomalies in fetuses affected by increased nuchal translucency(iNT >99Th centile), cystic hygroma (CH) and cases progressing to fetal hydrops (NIHF) in order to correlate this result to prognosis and perinatal fetal outcomes, improving patient's counseling. METHODS: From January 2020 to December 2023, first trimester screening according to FMF criteria were performed in "Maternal fetal medicine Unit" of Foggia's hospital. We studied and collected clinical data of fetuses affected by nuchal translucency >99th centile (iNT), CH and cases progressing to Fetal Hydrops (non-immune fetal hydrop, NIFH). In these selected cases, we evaluated fetal Karyotype to demonstrate the presence of chromosomal abnormalities, associated to fetal structural anomalies and different pregnancy outcome. RESULTS: We have evaluated 45 cases of iNT, CH, and NIFH, identified by ultrasound during first trimester screening. Of these 45 pregnant women, four were lost to follow-up. Of the 41 pregnancies, 20 cases (48.8%) delivered at our division with newborns discharged in good health. Of the 21 patients (51.2%) who miscarried, only two miscarried spontaneously; the other 19 decided on a medical termination of pregnancy (MTP). The 19 patients who decided for MTP showed genetic abnormalities and/or malformation. The 20 cases of fetuses discharged as healthy newborns were enrolled with the following diagnoses: 5 had a diagnosis of CH (20% of cases), 1 case of NIFH (5% of cases), and 14 of iNT (75% of cases). All these fetuses had a normal karyotype and no major malformations. CONCLUSIONS: Isolated CH diagnosis is more frequent than described in Literature (5 cases out of 12: 41.7%) and their prognosis are better than previously described, with the same outcomes of fetuses with iNT without associated anomalies. The possibility of early diagnosis of chromosomal anomalies, associated malformations or the evolution into hydrops is essential for a complete consultation.


Asunto(s)
Linfangioma Quístico , Medida de Translucencia Nucal , Primer Trimestre del Embarazo , Humanos , Femenino , Embarazo , Linfangioma Quístico/diagnóstico por imagen , Linfangioma Quístico/diagnóstico , Adulto , Resultado del Embarazo , Hidropesía Fetal/diagnóstico por imagen , Hidropesía Fetal/diagnóstico , Ultrasonografía Prenatal/métodos , Estudios Retrospectivos , Pronóstico
3.
J Appl Microbiol ; 129(5): 1261-1271, 2020 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-32445616

RESUMEN

AIMS: To conduct biological investigations and to evaluate the antimicrobial and antioxidant activities of the essential oils (EOs) extracted from Juniperus communis, J. scopulorum and J. horizontalis; to screen their mechanisms of action by conducting the cell membrane permeability assay (CMP); and to determine the possible cytotoxicity of the three EOs against human neuroblastoma cells (SH-SY5Y). METHODS AND RESULTS: The antifungal activity was tested against four phytopathogenic fungi (Monilinia fructicola, Aspergillus niger, Penicillium expansum and Botrytis cinerea). The antibacterial activity was evaluated against two Gram-positive (G+ve) (Bacillus megaterium and Clavibacter michiganensis) and three Gram-negative (G-ve) bacterial strains (Pseudomonas fluorescens, P. syringae pv. phaseolicola and Xanthomonas campestris). Results showed that the three tested EOs have antifungal activity against M. fructicola and P. expansum and effective antibacterial activity against P. syringae pv. phaseolicola and B. megaterium. Moreover, the three EOs were evaluated for their ability to inhibit the growth of SH-SY5Y cells with MTT assay. J. communis EO was the more effective with an IC50 of 53·7 µg ml-1 . The antioxidant capacity of the three EO did not differ as measured by the DPPH assay. CONCLUSIONS: The three tested juniper EOs showed promising antimicrobial and antioxidant activity and cytotoxic effects against human neuroblastoma cell line. SIGNIFICANCE AND IMPACT OF THE STUDY: The outfindings from this research showed promising antimicrobial effects of the three oils against the majority of the tested phytopathogens with a potential to utilize them as natural alternatives to synthetic drugs, the cause of global environmental problems, pathogen resistance and difficulty to control many post-harvest plant diseases.


Asunto(s)
Antiinfecciosos/farmacología , Antioxidantes/farmacología , Juniperus/química , Aceites Volátiles/farmacología , Aceites de Plantas/farmacología , Bacterias/efectos de los fármacos , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Hongos/efectos de los fármacos , Humanos , Juniperus/clasificación , Pruebas de Sensibilidad Microbiana , Enfermedades de las Plantas/microbiología
4.
Int J Immunopathol Pharmacol ; 26(1): 117-25, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-23527714

RESUMEN

Regulatory T cells (Tregs) are considered to be key immunomodulatory cells of the immune system and are increased in chronic lymphocytic leukemia (CLL). Rai stage 0 identifies patients with early stage CLL for which there is no effective intervention at the present time and a "wait and see" policy is usually adopted. Some biological and clinical studies have reported that green tea constituents, such as epigallocatechin-gallate (EGCG), have antitumor effects on hematologic malignancies including CLL. We report data on a clinical trial in which green tea extracts were given orally to 12 patients with stage 0 CLL and 12 healthy subjects. Ten patients and 10 controls completed the 6-month scheduled therapy. Two patients and 2 controls stopped therapy within 1 month because of tachycardia and epigastralgia. Eight out 10 evaluable patients (80 percent) showed a reduction of lymphocytosis and absolute number of circulating Tregs, as well. One patient (10 percent) had a stabilization of lymphocytosis and a reduction of Tregs, and 1 patient (10 percent) showed an increase of both lymphocytosis and Tregs. Only the non-responding patient progressed after 5 months from the end of green tea administration and chemotherapy was given. Interestingly, both IL-10 and TGF-beta serum levels declined throughout the green tea intake period, in both patients and controls. These data seem to indicate that green tea is able to modulate circulating Tregs in CLL patients with early stage of the disease. This can result in the control of lymphocytosis as well as in the prevention of disease progression.


Asunto(s)
Camellia sinensis , Factores Inmunológicos/farmacología , Leucemia Linfocítica Crónica de Células B/inmunología , Extractos Vegetales/farmacología , Linfocitos T Reguladores/efectos de los fármacos , Adulto , Anciano , Anciano de 80 o más Años , Recuento de Linfocito CD4 , Cafeína/análisis , Catequina/análogos & derivados , Catequina/análisis , Femenino , Humanos , Factores Inmunológicos/química , Interleucina-10/sangre , Masculino , Persona de Mediana Edad , Extractos Vegetales/química , Linfocitos T Reguladores/inmunología , Factor de Crecimiento Transformador beta/sangre
5.
Int J Immunopathol Pharmacol ; 24(4): 915-23, 2011.
Artículo en Inglés | MEDLINE | ID: mdl-22230398

RESUMEN

Regulatory T-cells (Tregs) constitute a small subset of cells involved in antitumour immunity and are generally increased in patients with chronic lymphocytic leukemia (CLL). No data is available on Tregs in monoclonal B-cell lymphocytosis (MBL), a disease entity characterized by less than 5000/microL circulating clonal B-cells in absence of other features of lymphoproliferative disorders. We used multicolour flow cytometry to evaluate the number of circulating Tregs in 56 patients with "clinical" MBL, 74 patients with previously untreated CLL and 40 healthy subjects. MBL patients showed a lower absolute number of Tregs, compared to CLL patients, but slightly higher than controls. Moreover, the absolute cell number of Tregs directly correlated both with more advanced Rai/Binet clinical stages and peripheral blood B-cell lymphocytosis. Of note, the absolute number of Tregs was found lower in MBL patients than in CLL patients staged as 0/A Rai/Binet. The study showed that Treg increase gradually from normal subjects to "clinical" MBL patients and are significantly higher in CLL patients as compared to MBL patients. Moreover, a significant direct relationship was found between higher Treg values and a higher tumor burden expressed by B-lymphocytosis or more advanced clinical stages. In light of this data, MBL seems to be a preliminary phase preceding CLL. The progressive increase of Treg numbers might contribute both to the clinical evolution of MBL to overt CLL and to CLL progression.


Asunto(s)
Linfocitos B/inmunología , Leucemia Linfocítica Crónica de Células B/inmunología , Linfocitosis/inmunología , Linfocitos T Reguladores/inmunología , Adulto , Anciano , Anciano de 80 o más Años , Estudios de Casos y Controles , Femenino , Citometría de Flujo , Humanos , Italia , Recuento de Linfocitos , Masculino , Persona de Mediana Edad
6.
Int J Immunopathol Pharmacol ; 22(4): 1097-104, 2009.
Artículo en Inglés | MEDLINE | ID: mdl-20074474

RESUMEN

We evaluated the pro-apoptotic activity of Verbena officinalis essential oil and of its main component citral, on lymphocytes collected from normal blood donors and patients with chronic lymphocytic leukemia (CLL). The number of apoptotic cells was greater in CLL patients than in healthy subjects at all different times of incubation (4, 8 and 24 hours) for samples treated with Verbena officinalis essential oil (A) and citral (B) as well vs controls at different concentrations (0.1% and 0.01%). The greater pro-apoptotic ability was shown by both essential oil of Verbena officinalis and citral at lower concentrations (after 4 h A 0.1%: 17.8% vs 37.1%; A 0.01%: 15.8% vs 52%; B 0.1%: 18.4% vs 46.4%; B 0.01%: 15.8% vs 54.2%; after 8 h A 0.1%: 23% vs 38%; A 0.01%: 22.2% vs 55%; B 0.1%: 32% vs 42.2%; B 0.01%: 22% vs 54.3%; after 24 h A 0.1%: 5% vs 20.7%; A 0.01%: 25.8% vs 47.2%; B 0.1%: 18.4% vs 46.4%; B 0.01%: 15.8% vs 54.2%). Patients carrying deletion 17p13 (p53 mutation) showed a reduced ability to undergo apoptosis with respect to patients with other genomic aberrations or normal karyotype. The proapoptotic activity of Verbena officinalis essential oil and citral is thought to be due to a direct procaspase 3 activation. These data further support evidence that indicate natural compounds as a possible lead structure to develop new therapeutic agents.


Asunto(s)
Antineoplásicos Fitogénicos/farmacología , Apoptosis/efectos de los fármacos , Leucemia Linfocítica Crónica de Células B/patología , Linfocitos/efectos de los fármacos , Monoterpenos/farmacología , Aceites Volátiles/farmacología , Aceites de Plantas/farmacología , Verbena , Monoterpenos Acíclicos , Adulto , Anciano , Antineoplásicos Fitogénicos/química , Antineoplásicos Fitogénicos/aislamiento & purificación , Apoptosis/genética , Estudios de Casos y Controles , Caspasa 3/metabolismo , Células Cultivadas , Relación Dosis-Respuesta a Droga , Activación Enzimática , Femenino , Humanos , Hibridación Fluorescente in Situ , Cariotipificación , Leucemia Linfocítica Crónica de Células B/enzimología , Leucemia Linfocítica Crónica de Células B/genética , Linfocitos/enzimología , Linfocitos/patología , Masculino , Persona de Mediana Edad , Monoterpenos/aislamiento & purificación , Mutación , Aceites Volátiles/química , Componentes Aéreos de las Plantas , Aceites de Plantas/química , Factores de Tiempo , Proteína p53 Supresora de Tumor/genética , Verbena/química
7.
J Endocrinol ; 120(3): 465-72, 1989 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-2926313

RESUMEN

The main objective of the present study was to analyse the hormonal dependence of the metrial gland formed in pseudopregnant animals following massive decidualization. On day 13 of pseudopregnancy (when the metrial gland reaches its maximal development) animals were ovariectomized and given s.c. implants of oestradiol and/or progesterone. A new implant technique for oestradiol delivery is described which provides circulating concentrations of oestradiol in the physiological range. In addition, we extended our previous work concerning oestradiol receptor and progesterone receptor concentrations in the metrial gland of pseudopregnant rats. The low oestradiol receptor concentration which we previously reported up to day 17 was maintained until the end of pseudopregnancy (day 21-1.5 fmol/micrograms DNA), whereas the progesterone receptor concentration remained raised (congruent to 3.5 fmol/micrograms DNA) from day 13 to day 19 and then decreased on day 21. The correlation of metrial gland weight and kinetics of the tissue oestradiol and progesterone receptors contents with the circulating oestradiol and progesterone concentrations lead to the following conclusions. First, the maintenance of the metrial gland is strictly progesterone-dependent. It is unlike the deciduoma which regresses spontaneously, even in the presence of progesterone. Secondly, the production of oestradiol receptor, but not of progesterone receptor, appears to be repressed in the metrial gland under the influence of progesterone. Thus, the tissue retains its ability to respond to progesterone because of a high concentration of progesterone receptor. It is difficult to attribute this high tissue progesterone receptor concentration to oestradiol stimulation since, even at low levels, oestradiol induces tissue regression. We suggest that the high progesterone receptor concentration could be due to constitutive (basal) progesterone receptor production.


Asunto(s)
Glándula Metrial/fisiología , Receptores de Estradiol/análisis , Receptores de Progesterona/análisis , Animales , Estradiol/sangre , Femenino , Glándula Metrial/análisis , Ovariectomía , Progesterona/sangre , Seudoembarazo , Ratas , Ratas Endogámicas , Factores de Tiempo
8.
Phytochemistry ; 41(2): 531-6, 1996 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-8821433

RESUMEN

From wild garlic Allium ursinum three new flavonoid glycosides were identified as kaempferol 3-O-beta-neohesperidoside-7-O-[2-O-(trans-p-coumaroyl)]-beta -D- glucopyranoside, kaempferol 3-O-beta-neohesperidoside-7-O-[2-O-(trans-feruloyl)]-beta-D- glucopyranoside, kaempferol 3-O-beta-neohesperidoside-7-O-[2-O-(trans-p-coumaroyl)-3-O-b eta-D- glucopyranosyl]-beta-D-glucopyranoside and characterized as the peracetates. Additionally, two known flavonoid glycosides kaempferol 3-O-beta-glucopyranoside and kaempferol 3-O-beta-neohesperidoside were isolated. The isolated compounds showed an inhibition of human platelet aggregation.


Asunto(s)
Flavonoides/química , Flavonoides/farmacología , Ajo/química , Extractos Vegetales/química , Extractos Vegetales/farmacología , Plantas Medicinales , Inhibidores de Agregación Plaquetaria/química , Inhibidores de Agregación Plaquetaria/farmacología , Plaquetas/efectos de los fármacos , Secuencia de Carbohidratos , Flavonoides/aislamiento & purificación , Glicósidos/química , Glicósidos/aislamiento & purificación , Glicósidos/farmacología , Humanos , Espectroscopía de Resonancia Magnética , Datos de Secuencia Molecular , Extractos Vegetales/aislamiento & purificación , Inhibidores de Agregación Plaquetaria/aislamiento & purificación , Espectrometría de Masa Bombardeada por Átomos Veloces , Espectrofotometría Ultravioleta
9.
Phytochemistry ; 44(5): 949-57, 1997 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-9115694

RESUMEN

An investigation of the extracts from Allium neapolitanum has led to the isolation of 13 flavonoid glycosides, based on kaempferol, quercetin and isorhamnetin. Four of them are new compounds and have been identified as: kaempferol 3-O-[[2-O-alpha-L-rhamnopyranosyl-4-O-beta-D-glucopyranosyl]-beta-D- glucopyranoside]], isorhamnetin 3-O-[[2-O-alpha-L-rhamnopyranosyl-6-O-beta- D-glucopyranosyl]-beta-D-glucopyranoside], isorhamnetin 3-O-[[2-O-alpha-L-rhamnopyranosyl-6-O-beta-D-glycopyranosyl] beta-D-glucopyranoside]-7-O-beta-D-glucopyranoside and isorhamnetin 3-O-[[2-O-alpha-L-rhamnopyranosyl-6-O-beta-D-gentiobiosyl]- beta-D-glucopyranoside]]. The isolated compounds were evaluated for their anti-aggregation human platelet activity.


Asunto(s)
Allium/química , Flavonoides/química , Flavonoides/farmacología , Extractos Vegetales/química , Extractos Vegetales/farmacología , Inhibidores de Agregación Plaquetaria/química , Inhibidores de Agregación Plaquetaria/farmacología , Flavonoides/aislamiento & purificación , Glicósidos/química , Glicósidos/aislamiento & purificación , Glicósidos/farmacología , Humanos , Italia , Espectroscopía de Resonancia Magnética , Extractos Vegetales/aislamiento & purificación , Agregación Plaquetaria/efectos de los fármacos , Inhibidores de Agregación Plaquetaria/aislamiento & purificación
10.
Phytochemistry ; 30(5): 1635-7, 1991.
Artículo en Inglés | MEDLINE | ID: mdl-1367339

RESUMEN

From the bark of Uncaria guianensis, two new quinovic acid glycosides, quinovic acid 3 beta-O-beta-D-quinovopyranoside and quinovic acid 3 beta-O-beta-D-fucopyranosyl-(27----1)-beta-D-glucopyranosylester, have been isolated, in addition to known quinovic acid 3 beta-O-[beta-D-glucopyranosyl-(1----3)-beta-D-fucopyranosyl]-(27----1)- beta-D-glucopyranosylester and quinovic acid 3 beta-O-beta-D-fucopyranoside. Their structures were elucidated by spectral and chemical studies.


Asunto(s)
Glicósidos/aislamiento & purificación , Plantas Medicinales/análisis , Triterpenos/aislamiento & purificación , Secuencia de Carbohidratos , Glicósidos/química , Espectroscopía de Resonancia Magnética , Datos de Secuencia Molecular , Triterpenos/química
11.
Anticancer Res ; 21(4A): 2457-61, 2001.
Artículo en Inglés | MEDLINE | ID: mdl-11724307

RESUMEN

Uncaria tomentosa, also known as "Uña de gato", is a Rubiaceae species widely used in South-American folk medicine for the treatment of cancer, arthritis, gastritis and epidemic diseases. Extracts of the plant have been shown to possess cytostatic and anti-inflammatory activity as well as mutagenic and antimutagenic properties. However, to date no studies have been carried out to verify the direct antitumor activity of the extracts. The present study investigates the effects of some extracts and their chromatographic fractions from the bark of U. tomentosa on the growth of a human breast cancer cell line (MCF7). Our data indicated that, in addition to the antimutagenic activity, U. tomentosa extracts and fractions exert a direct antiproliferative activity on MCF7. The bioassay-directed fractionation from barks and leaves resulted in the isolation of two active fractions, which displayed an IC50 of 10 mg/ml and 20 mg/ml, respectively and an antiproliferative effect, with about 90% of inhibition at a concentration of 100 mg/ml.


Asunto(s)
Neoplasias de la Mama/tratamiento farmacológico , Uña de Gato , Fitoterapia , Extractos Vegetales/farmacología , Neoplasias de la Mama/patología , Uña de Gato/química , División Celular/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Ensayos de Selección de Medicamentos Antitumorales , Inhibidores de Crecimiento/farmacología , Humanos , Concentración 50 Inhibidora , Metanol/química , Corteza de la Planta/química , Extractos Vegetales/toxicidad , Hojas de la Planta/química , Células Tumorales Cultivadas , Agua/química
12.
J Agric Food Chem ; 47(9): 3682-4, 1999 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-10552704

RESUMEN

In addition to some histological observations, the chemical composition of Carica candicans Gray (Caricaceae) fruit and seeds, a plant common in Peruvian nutritional habits, was determined. The fruit contains high amounts of total proteins (8.2% on dry weight basis) and carbohydrates (70.1%) and appreciable contents of vitamin C and minerals. The oil extracted from seeds is in high amount (41. 6%). The fatty acid composition, with a prevalence of oleic, palmitic, and linoleic acids, suggests a possible use of this oil in alimentation.


Asunto(s)
Frutas/química , Semillas/química , Ácido Ascórbico/análisis , Carbohidratos/análisis , Fibras de la Dieta/análisis , Ácidos Grasos/análisis , Minerales/análisis , Valor Nutritivo , Perú , Aceites de Plantas/química , Proteínas de Plantas/análisis
13.
Food Chem Toxicol ; 32(9): 783-8, 1994 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-7927074

RESUMEN

Coloured polyethylene terephthalate (PET) bottles for carbonated beverages were studied for potential migration of genotoxic compounds. A combined approach, using physicochemical methods and a bacterial short-term mutagenicity test (Ames test) was followed. Standard EEC and US FDA tests for total migration of non-volatile migrant compounds into distilled water were performed, together with modified tests, where freeze-drying instead of evaporation of water was used, in order to measure both volatile and non-volatile chemicals. Gas chromatography-mass spectrometry (GC-MS) analysis was performed on these residues. PET bottles filled with naturally carbonated mineral water were also used for long-term total organic carbon (TOC) and mutagenicity migration studies (up to 6 months' storage). Total migration results for PET bottles were within the EEC and US FDA limits. The use of freeze-drying for the elimination of water enabled much higher total migration data (higher than the limits) to be revealed. Some potentially genotoxic compounds (acetaldehyde, dimethyl terephthalate, terephthalic acid) were identified in these migrant compounds by GC-MS analysis. The tests for TOC migration gave a maximum value after 2 wk storage and the mutagenicity tests on non-volatile migrant compounds gave always negative results.


Asunto(s)
Aguas Minerales , Mutágenos/análisis , Plásticos , Tereftalatos Polietilenos , Carbono/análisis , Unión Europea , Manipulación de Alimentos , Cromatografía de Gases y Espectrometría de Masas , Pruebas de Mutagenicidad , Mutágenos/toxicidad , Salmonella typhimurium/efectos de los fármacos , Solubilidad , Estados Unidos , United States Food and Drug Administration
14.
J Ethnopharmacol ; 39(1): 39-51, 1993 May.
Artículo en Inglés | MEDLINE | ID: mdl-8331961

RESUMEN

This paper reports the results of an investigation carried out from April 1990 to October 1991 in the Amalfitan Coast, Salerno Province (Southern Italy). One hundred and twenty-five species are used in traditional medicine, belonging to 53 families; some of these were unknown in Italian phytotherapy or present an unknown therapeutic use. For each species, the vernacular name, the formulation and the popular use are presented.


Asunto(s)
Medicina Tradicional , Extractos Vegetales/uso terapéutico , Humanos , Italia , Extractos Vegetales/aislamiento & purificación , Plantas Medicinales/clasificación
15.
J Ethnopharmacol ; 36(2): 113-25, 1992 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-1608267

RESUMEN

A study on the wild and cultivated medicinal plants used in the Peninsula Sorrentina (southern Italy) is reported; 129 plants distributed among 53 different families beloning to Pteridophytes, Gymnosperms and Angiosperms are used in the treatment of various human diseases. The use of some species is reported for the first time in this paper.


Asunto(s)
Plantas Medicinales , Humanos , Italia , Medicina Tradicional
16.
J Ethnopharmacol ; 38(1): 63-77, 1993 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-8479203

RESUMEN

Mutagenic and antimutagenic activities of extracts and chromatographic fractions of Uncaria tomentosa bark are reported. The plant extracts and fractions show no mutagenic effect in different strains of Salmonella typhimurium with and without metabolic activation. However, the plant extracts and fractions show a protective antimutagenic effect in vitro against photomutagenesis induced by 8-methoxy-psoralen (8-MOP) plus UVA in S. typhimurium TA 102. A decoction of U. tomentosa ingested daily for 15 days by a smoker decreased the mutagenicity induced in S. typhimurium TA98 and TA100 by the subject's urine.


Asunto(s)
Antimutagênicos/farmacología , Mutágenos/toxicidad , Extractos Vegetales/farmacología , Adulto , Animales , Antimutagênicos/metabolismo , Cloroformo/química , Humanos , Masculino , Metanol/química , Metoxaleno , Pruebas de Mutagenicidad , Mutágenos/metabolismo , Extractos Vegetales/toxicidad , Extractos Vegetales/orina , Plantas Medicinales , Ratas , Ratas Sprague-Dawley , Salmonella typhimurium/efectos de los fármacos , Salmonella typhimurium/efectos de la radiación , Fumar/orina , Solubilidad , Rayos Ultravioleta
17.
J Physiol Pharmacol ; 65(6): 859-66, 2014 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-25554990

RESUMEN

Cymbopogon martinii (Cm.Cr) is traditionally used in south Asian communities for the management of multiple ailments including gastrointestinal, respiratory and vascular disorders and the present study was undertaken to validate these folkloric uses. The application of a methanol extract of the plant (Cm.Cr) to isolated rabbit jejunum preparation exhibited relaxation through decrease in magnitude and frequency of spontaneous contractions. The Cm.Cr also exerted relaxant effect on high K(+) (80 mM) induced contractions in isolated rabbit jejunum preparations. The Cm.Cr and its dichloromethane (Cm.Dcm) and aqueous (Cm.Aq) fractions also caused concentration-dependent relaxation in spontaneous and K(+) (80 mM) induced contractions which are comparables to effects produced by verapamil. Cm.Cr caused shifting of the Ca(2+)-curves toward right, suggesting the presence of a Ca(2+) channel blocking activity. Subsequently, Cm.Cr, Cm.Dcm and Cm.Aq caused relaxation of CCh (1 µM) and K(+) (80 mM) induced contractions in isolated rabbit tracheal preparations, suggesting that the observed relaxant effect can be mediated through antimuscarinic and/or Ca(2+) channel blocking activities. Cm.Cr tested against phenylephrine (PE; 1 µM) and K(+) (80 mM) induced contractions exhibited partial relaxation of isolated rabbit aortic preparations. The above-mentioned studies provided a scientific basis for the folkloric use of Cymbopogon martini in the management of multiple ailments in traditional systems of medicines.


Asunto(s)
Broncodilatadores/farmacología , Cymbopogon , Parasimpatolíticos/farmacología , Extractos Vegetales/farmacología , Vasodilatadores/farmacología , Animales , Aorta Torácica/efectos de los fármacos , Aorta Torácica/fisiología , Bloqueadores de los Canales de Calcio/farmacología , Femenino , Técnicas In Vitro , Yeyuno/efectos de los fármacos , Yeyuno/fisiología , Masculino , Hojas de la Planta , Conejos , Tráquea/efectos de los fármacos , Tráquea/fisiología , Verapamilo/farmacología
18.
J Physiol Pharmacol ; 64(4): 479-84, 2013 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-24101394

RESUMEN

The present study was undertaken to validate some of the folkloric claims about the effectiveness of the use of a Myrtus communis L. crude methanol extract (Mc.Cr) in gastrointestinal, respiratory and vascular diseases. Mc.Cr caused complete relaxation of spontaneous and K⁺ (80 mM)-induced contractions in isolated rabbit jejunum. It caused right ward parallel shift of calcium concentration response curves. Mc.Cr exhibited relaxant effect on CCh- and K⁺ (80 mM)-induced contractions in isolated rabbit tracheal preparations. Furthermore, Mc.Cr caused relaxation of phenylephrine (1 µM)- and K⁺ (80 mM)-induced contractions in isolated rabbit aorta preparations. These effects were similar to verapamil, a standard calcium channel blocker. These findings could be the basis for explaining the spasmolytic, bronchodilator and vasodilator activities of the extract, through a possible calcium channel blocking activity.


Asunto(s)
Broncodilatadores/farmacología , Bloqueadores de los Canales de Calcio/farmacología , Myrtus , Parasimpatolíticos/farmacología , Extractos Vegetales/farmacología , Vasodilatadores/farmacología , Animales , Aorta Torácica/efectos de los fármacos , Aorta Torácica/fisiología , Canales de Calcio/fisiología , Técnicas In Vitro , Yeyuno/efectos de los fármacos , Yeyuno/fisiología , Contracción Muscular/efectos de los fármacos , Componentes Aéreos de las Plantas , Conejos , Tráquea/efectos de los fármacos , Tráquea/fisiología
19.
Mini Rev Med Chem ; 11(6): 492-502, 2011 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-21561407

RESUMEN

Human leukemia results from multiple mutations that lead to abnormalities in the expressions and functions of genes that maintain the delicate balance between proliferation, differentiation and apoptosis. Continued research on the molecular aspects of leukemia cells has resulted in the developments of several potentially useful therapeutic agents. Discovery of new cellular and/or molecular pathways enabling innate or acquired resistance of cancers to current chemotherapeutics to be overcome is therefore of crucial importance if one wants to efficiently combat those cancers associated with dismal prognoses. In this concern, natural compounds are regarded as new chemical entities for the development of drugs against various pharmacological targets, including cancer, and, above all, leukemia.


Asunto(s)
Leucemia/tratamiento farmacológico , Alcaloides/química , Alcaloides/uso terapéutico , Cumarinas/química , Cumarinas/uso terapéutico , Flavonoides/química , Flavonoides/uso terapéutico , Humanos , Fenoles/química , Fenoles/uso terapéutico , Polifenoles , Terpenos/química , Terpenos/uso terapéutico
20.
Mini Rev Med Chem ; 11(6): 486-91, 2011 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-21561408

RESUMEN

On the basis of harmine and 1-methoxy-canthin-6-one chemical structures, a series of novel 1,4-disubstituted and 1,4,9-trisubstituted ß-carbolines and tetracyclic derivatives were designed and synthesized. Cytotoxic activities of these compounds in vitro were investigated in a human tumor cell line panel. Almost all compounds demonstrated interesting cytotoxic activities in particular against prostate cancer cells PC-3 with IC50 in the low micromolar range. Compound X was found to be the most potent one with IC50 value of 8.0 µM; this suggests further studies with models of prostate cancer.


Asunto(s)
Antineoplásicos/síntesis química , Carbolinas/química , Antineoplásicos/farmacocinética , Antineoplásicos/toxicidad , Carbolinas/farmacocinética , Carbolinas/toxicidad , Línea Celular Tumoral , Ensayos de Selección de Medicamentos Antitumorales , Humanos
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