Genetic parameters for automatically-measured vaginal temperature, respiration efficiency, and other thermotolerance indicators measured on lactating sows under heat stress conditions.

BACKGROUND: Genetic selection based on direct indicators of heat stress could capture additional mechanisms that are involved in heat stress response and enable more accurate selection for more heat-tolerant individuals. Therefore, the main objectives of this study were to estimate genetic parameters for various heat stress indicators in a commercial population of Landrace × Large White lactating sows measured under heat stress conditions. The main indicators evaluated were: skin surface temperatures (SST), automatically-recorded vaginal temperature (TV), respiration rate (RR), panting score (PS), body condition score (BCS), hair density (HD), body size (BS), ear size, and respiration efficiency (Reff). RESULTS: Traits based on TV presented moderate heritability estimates, ranging from 0.15 ± 0.02 to 0.29 ± 0.05. Low heritability estimates were found for SST traits (from 0.04 ± 0.01 to 0.06 ± 0.01), RR (0.06 ± 0.01), PS (0.05 0.01), and Reff (0.03 ± 0.01). Moderate to high heritability values were estimated for BCS (0.29 ± 0.04 for caliper measurements and 0.25 ± 0.04 for visual assessments), HD (0.25 ± 0.05), BS (0.33 ± 0.05), ear area (EA; 0.40 ± 0.09), and ear length (EL; 0.32 ± 0.07). High genetic correlations were estimated among SST traits (> 0.78) and among TV traits (> 0.75). Similarly, high genetic correlations were also estimated for RR with PS (0.87 ± 0.02), with BCS measures (0.92 ± 0.04), and with ear measures (0.95 ± 0.03). Low to moderate positive genetic correlations were estimated between SST and TV (from 0.25 ± 0.04 to 0.76 ± 0.07). Low genetic correlations were estimated between TV and BCS (from - 0.01 ± 0.08 to 0.06 ± 0.07). Respiration efficiency was estimated to be positively and moderately correlated with RR (0.36 ± 0.04), PS (0.56 ± 0.03), and BCS (0.56 ± 0.05 for caliper measurements and 0.50 ± 0.05 for the visual assessments). All other trait combinations were lowly genetically correlated. CONCLUSIONS: A comprehensive landscape of heritabilities and genetic correlations for various thermotolerance indicators in lactating sows were estimated. All traits evaluated are under genetic control and heritable, with different magnitudes, indicating that genetic progress is possible for all of them. The genetic correlation estimates provide evidence for the complex relationships between these traits and confirm the importance of a sub-index of thermotolerance traits to improve heat tolerance in pigs.

Evaluating phenotypes associated with heat tolerance and identifying moderate and severe heat stress thresholds in lactating sows housed in mechanically or naturally ventilated barns during the summer under commercial conditions.

An accurate understanding of heat stress (HS) temperatures and phenotypes that indicate HS tolerance is necessary to improve swine HS resilience. Therefore, the study objectives were 1) to identify phenotypes indicative of HS tolerance, and 2) to determine moderate and severe HS threshold temperatures in lactating sows. Multiparous (4.10 ± 1.48) lactating sows and their litters (11.10 ± 2.33 piglets/litter) were housed in naturally ventilated (n = 1,015) or mechanically ventilated (n = 630) barns at a commercial sow farm in Maple Hill, NC, USA between June 9 and July 24, 2021. In-barn dry bulb temperatures (TDB) and relative humidity were continuously recorded for naturally ventilated (26.38 ± 1.21 °C and 83.38 ± 5.40%, respectively) and mechanically ventilated (26.91 ± 1.80 °C and 77.13 ± 7.06%, respectively) barns using data recorders. Sows were phenotyped between lactation days 11.28 ± 3.08 and 14.25 ± 3.26. Thermoregulatory measures were obtained daily at 0800, 1200, 1600, and 2000 h and included respiration rate, and ear, shoulder, rump, and tail skin temperatures. Vaginal temperatures (TV) were recorded in 10 min intervals using data recorders. Anatomical characteristics were recorded, including ear area and length, visual and caliper-assessed body condition scores, and a visually assessed and subjective hair density score. Data were analyzed using PROC MIXED to evaluate the temporal pattern of thermoregulatory responses, phenotype correlations were based on mixed model analyses, and moderate and severe HS inflection points were established by fitting TV as the dependent variable in a cubic function against TDB. Statistical analyses were conducted separately for sows housed in mechanically or naturally ventilated barns because the sow groups were not housed in each facility type simultaneously. The temporal pattern of thermoregulatory responses was similar for naturally and mechanically ventilated barns and several thermoregulatory and anatomical measures were significantly correlated with one another (P < 0.05), including all anatomical measures as well as skin temperatures, respiration rates, and TV. For sows housed in naturally and mechanically ventilated facilities, moderate HS threshold TDB were 27.36 and 26.69 °C, respectively, and severe HS threshold TDB were 29.45 and 30.60 °C, respectively. In summary, this study provides new information on the variability of HS tolerance phenotypes and environmental conditions that constitute HS in commercially housed lactating sows.

Climate change and the associated increase in global temperatures have a well-described negative impact on swine production. Therefore, improving swine heat stress resilience is of utmost importance to reduce the deleterious effects of heat stress on swine health, performance, and welfare. Genomic selection for heat stress resilience may be a viable strategy to improve swine productivity in a changing climate. However, identifying environmental conditions that constitute heat stress and deriving novel traits that can be easily collected on farm and provide accurate and precise predictions of heat stress tolerance is a necessary step. The present study demonstrated that housing conditions had a limited influence on heat stress tolerance phenotypes, several anatomical and thermoregulatory measures were correlated, and housing conditions impacted heat stress threshold temperatures. Results from this study may be applied to large-scale phenotyping initiatives to develop or refine genomic selection indexes for heat stress resilience in pigs.

Evaluation of essential fatty acids in lactating sow diets on sow reproductive performance, colostrum and milk composition, and piglet survivability.

Mixed parity sows (n = 3,451; PIC, Hendersonville, TN; parities 2 through 9) and their litters were used to evaluate the effects of essential fatty acid (EFA) intake on sow reproductive performance, piglet growth and survivability, and colostrum and milk composition. Our hypothesis, like observed in earlier research, was that increasing linoleic acid (LA) and α-linolenic acid (ALA) would improve sow and litter performance. At approximately day 112 of gestation, sows were randomly assigned within parity groups to 1 of 4 corn-soybean meal-wheat-based lactation diets that contained 0.5 (Control) or 3% choice white grease (CWG), 3% soybean oil (SO), or a combination of 3% soybean oil and 2% choice white grease (Combination). Thus, sows were provided diets with low LA and ALA in diets with CWG or high LA and ALA in diets that included soybean oil. Sows received their assigned EFA treatments until weaning and were then fed a common gestation and lactation diet in the subsequent reproductive cycle. Average daily feed intake during the lactation period increased (P < 0.05) for sows fed the Combination and CWG diets compared with sows fed the Control or SO diet. However, daily LA and ALA intakes of sows fed the Combination and SO diets were still greater (P < 0.05) than those of sows fed 0.5 or 3% CWG. Overall, sows consuming high EFA from the Combination or SO diets produced litters with heavier (P < 0.05) piglet weaning weights and greater (P < 0.05) litter ADG when compared with litters from sows fed diets with CWG that provided low EFA. Despite advantages in growth performance, there was no impact of sow EFA intake on piglet survivability (P > 0.10). Additionally, lactation diet EFA composition did not influence sow colostrum or milk dry matter, crude protein, or crude fat content (P > 0.10). However, LA and ALA content in colostrum and milk increased (P < 0.05) in response to elevated dietary EFA from SO. There was no evidence for differences (P > 0.10) in subsequent sow reproductive or litter performance due to previous lactation EFA intake. In conclusion, increased LA and ALA intake provided by soybean oil during lactation increased overall litter growth and pig weaning weights, reduced sow ADFI, but did not affect piglet survivability or subsequent performance of sows.

Supplemental fat sources are an effective and widely accepted strategy to increase energy density of sow lactation diets that can also provide essential fatty acids such as linoleic acid (LA) and α-linolenic acid (ALA). Currently, the effects of supplemental LA and ALA provided shortly before farrowing on colostrum and milk composition are not fully understood. Additionally, the influence of elevated LA and ALA provided in sow lactation diets on litter growth and survivability responses has not been extensively evaluated. Therefore, this trial was conducted to evaluate the effects of fat sources providing low and high LA and ALA intake on sow performance, litter growth and survivability, colostrum and milk composition, and subsequent reproductive performance. Overall, sows consuming diets with high LA and ALA provided by soybean oil produced litters with heavier piglet weaning weights and greater litter average daily gain when compared with sows consuming diets with low LA and ALA content. Increasing LA and ALA by added soybean oil also increased their content in colostrum and milk. However, there was no influence of sow LA and ALA intake on litter survivability or subsequent reproductive performance of sows.

Proposed multidimensional pain outcome methodology to demonstrate analgesic drug efficacy and facilitate future drug approval for piglet castration.

Castration of male piglets in the United States is conducted without analgesics because no Food and Drug Administration (FDA) approved products are labeled for pain control in swine. The absence of approved products is primarily due to a wide variation in how pain is measured in suckling piglets and the lack of validated pain-specific outcomes individually indistinct from other biological responses, such as general stress or inflammation responses with cortisol. Simply put, to measure pain mitigation, measurement of pain must be specific, quantifiable, and defined. Therefore, given the need for mitigating castration pain, a consortium of researchers, veterinarians, industry, and regulatory agencies was formed to identify potential animal-based outcomes and develop a methodology, based on the known scientific research, to measure pain and the efficacy of mitigation strategies. The outcome-based measures included physiological, neuroendocrine, behavioral, and production parameters. Ultimately, this consortium aims to provide a validated multimodal methodology to demonstrate analgesic drug efficacy for piglet castration.Measurable outcomes were selected based on published studies suggesting their validity, reliability, and sensitivity for the direct or indirect measurement of pain associated with surgical castration in piglets. Outcomes to be considered are observation of pain behaviors (i.e. ethogram defined behaviors and piglet grimace scale), gait parameters measured with a pressure mat, infrared thermography of skin temperature of the cranium and periphery of the eye, and blood biomarkers. Other measures include body weight and mortality rate.This standardized measurement of the outcome variable's primary goal is to facilitate consistency and rigor by developing a research methodology utilizing endpoints that are well-defined and reliably measure pain in piglets. The resulting methodology will facilitate and guide the evaluation of the effectiveness of comprehensive analgesic interventions for 3- to 5-day-old piglets following surgical castration.

Effects of group-size-floor space allowance during the nursery phase of production on future litter size and retention of sows through three parities.

We previously reported that reduced floor space allowance caused by increasing the number of gilts per pen decreased growth and affected blood chemistry and immunology. The current objective was to determine effects of nursery group-size-floor space allowance on future litter sizes and retention in the breeding herd through three parities in sows. A 3 × 3 factorial arrangement of treatments was employed with 2,537 gilts classified as large (6.92 ± 0.06 kg), medium (5.60 ± 0.06 kg), or small (4.42 ± 0.06 kg), and placed in nursery pens of 14, 11, or 8 pigs to allow 0.15, 0.19, or 0.27 m2 floor space/pig, respectively. After the nursery and grow-finish periods, 1,453 gilts selected for breeding were relocated to one of 11 sow farms. Total litter size and pigs born alive increased (P < 0.01) with increasing parity and total litter size was 12.94, 13.28, and 13.99 (SE = 0.13) and pigs born alive was 12.21, 12.64, and 13.23 (SE = 0.11) for Parities 1, 2, and 3, respectively. There was a tendency (P = 0.08) for a quadratic relationship of group-size-floor space allowance and total litter size (13.39, 13.54, and 13.27 [SE = 0.13] for gilts allowed 0.15, 0.19, or 0.27 m2 floor space/pig, respectively). A linear effect of size of pig at weaning (P = 0.03) on pigs born dead was detected and was 0.64, 0.75, and 0.75, for small, medium, and large size pigs, respectively. There was no effect of group-size-floor space allowance on the percentages of gilts completing zero (P = 0.36), one (P = 0.35), two (P = 0.32), or three (P = 0.50) parities. In contrast, the percentage of small gilts that failed to complete one parity was greater (P < 0.05) and the percentage completing one parity (P < 0.05) was less than for either large or medium gilts. Abortion rate was greater (P < 0.01) in gilts classified as small (2.51%) or medium (1.36%) at weaning compared with those classified as large (0.20%). Size at weaning did not affect the proportion of gilts completing two (P = 0.88) or three (P = 0.72) parities. Group-size-floor space allowance during the nursery phase of production did not have remarkable effects on future litter sizes or retention in sows. Likewise, size of pig at weaning did not affect litter size and pigs born alive. Compared with larger pigs, however, more pigs classified as small at weaning and entering the breeding herd did not complete a parity and displayed a greater abortion rate.

Effect of suckling intensity of primiparous sows on production performance during current and subsequent parities1.

This study was conducted to evaluate the effects of suckling intensity (litter size and lactation length) to primiparious sows on production performance during current and subsequent parities. Upon farrowing, 115 primiparous sows (farrowing weight: 222.7 ± 20.0 kg) were initially allotted to 4 treatments in a 2 × 2 factorial arrangement with 2 litter sizes: 10 and 13 piglets (LS10 vs. LS13), and 2 lactation lengths: 21 and 27 d (LL21 vs. LL27). Upon weaning, sows were rebred and those farrowed successfully (n = 66) kept 10 piglets and weaned at 21 d in the second parity. Sows were fed ad libitum during lactation in both parities. Feed intake, BW loss, backfat loss, litter size, and litter weight gain during lactation in both parities were determined. Litter weight gain in LS13 was greater (P < 0.05) than that in LS10 (54.4 vs. 47.7 kg) during the first lactation. Sows in LS13 had a greater (P < 0.05) BW loss than sows in LS10 (24.1 vs. 17.4 kg). Body weight loss was not different between LL27 and LL21. Sows in LS13 tended to have a greater (P = 0.075) removal rate than those in LS10 (47.5 vs. 32.2%). Sows in LL27 had a smaller (P < 0.05) removal rate than those in LL21 (28.0 vs. 51.7%). In the second parity, gestation BW gain in LL27 tended to be greater (P = 0.098) than that in LL21 when the previous litter size was 10 piglets (56.1 vs. 33.2 kg). Litter performance and feed intake of sows were not affected by previous litter size, lactation length, and their interaction. The farrowing weight, farrowing body protein and lipid, body weight loss was not different between LS13 and LS10, whereas backfat loss in LS13 was smaller (P < 0.05) than that in LS10 during the second lactation (0.9 vs. 2.4 mm). The predicted body lipid loss in LS13 was also smaller than that in LS10 (2.3 vs. 5.3 kg) during the second lactation. Sows in LL27 had a smaller (P < 0.05) BW loss and body lipid loss during the second lactation than sows in LL21 (4.0 vs. 9.0 kg; 2.3 vs. 4.8 kg). The concentration of milk fat in LL27 was smaller (P < 0.05) than that in LL21 (7.9 vs. 9.1%). In conclusion, increasing suckling intensity to primiparous sows increased litter weight gain but increasing litter size reduced piglet ADG. Sow performance in the second lactation was not negatively affected by increasing suckling intensity of the first lactation. Interestingly, sows with an increased suckling intensity in the first lactation had reduced loss of body reserves in the second lactation.