Single-shot spatial light interference microscopy for dynamic monitoring of membrane fluctuations.

Single-shot spatial light interference microscopy (SS-SLIM) with a pair of non-polarizing beam splitters is proposed for substantially enhancing the speed and efficiency of conventional SLIM systems. Traditional methods are limited by the need for multiple-frame serial modulation and acquisition by spatial light modulators and detectors. Our approach integrates non-polarizing beam splitters to simultaneously capture four phase-shifted intensity images, increasing the imaging speed by at least fourfold while maintaining high quality. This capability is crucial for effectively monitoring the dynamic fluctuations of red blood cell membranes. Furthermore, the potential applications of the SS-SLIM system in biomedical research are demonstrated, particularly in scenarios requiring high temporal resolution and label-free imaging.

Direct evidence for the occurrence of indigenous cadmium-based nanoparticles in paddy soils.

Speciation of heavy metal-based nanoparticles (NPs) in paddy soils greatly determines their fate and potential risk towards food safety. However, quantitative understanding of such distinctive species remains challenging, because they are commonly presented at trace levels (e.g., sub parts-per-million) and extremely difficult to be fractionated in soil matrices. Herein, we propose a state-of-art non-destructive strategy for effective extraction and quantification of cadmium (Cd)-NPs - the most widespread heavy metal in paddy soils - by employing single particle inductively coupled plasma mass spectrometry (spICP-MS) and tetrasodium pyrophosphate (TSPP) as the extractant. Acceptable extraction efficiencies (64.7-80.4 %) were obtained for spiked cadmium sulfide nanoparticles (CdS-NPs). We demonstrate the presence of indigenous Cd-NPs in all six Cd-contaminated paddy soils tested, with a number concentration ranging from 2.20 × 108 to 3.18 × 109 particles/g, representing 17.0-50.4 % of the total Cd content. Furthermore, semi-spherical and irregular CdS-NPs were directly observed as an important form of the Cd-NPs in paddy soils, as characterized by transmission electron microscopy coupled with energy-dispersive X-ray spectroscopy (TEM-EDX). This research marks a significant step towards directly observing indigenous Cd-NPs at trace levels in paddy soil, offering a useful tool for quantitative understanding of the biogeochemical cycling of heavy metal-based NPs in complex matrices.

Deciphering fluorescent and molecular fingerprint of dissolved organic matter leached from microplastics in water.

Despite extensive research into the presence and behavior of microplastics (MPs) in the environment, limited attention has been given to the investigation of the characteristics of dissolved organic matter (DOM) that leaches from MPs (MPs-DOM). Herein, two frequently encountered plastic particles in aquatic environments, specifically polyethylene terephthalate (PET)- and polyethylene (PE)-MPs, were subjected to leaching in the aquatic settings for seven days, both in the absence of light and under UV irradiation. Measurements of dissolved organic carbon (DOC) indicated that UV exposure enhanced the liberation of DOM from PET-MPs, while PE-MPs did not exhibit such leaching. After UV treatment for seven days, the DOM released from PET-MPs increased by 25 times, while that from PE-MPs remained almost unchanged. Then, the molecular diversity and the evolving formation of DOM originating from different MPs were comprehensively analyzed with fluorescence excitation-emission matrix (EEM) and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS). Specifically, both PET- and PE-DOM exhibited three fluorescence signatures, with the predominant C1 (tryptophan-like) component showing a decline in PET-DOM and a rise in PE-DOM during aging. The FT-ICR-MS analysis unveiled that PET-DOM grew more recalcitrant under UV exposure, while PE-DOM became increasingly labile. In brief, UV irradiation influences MPs-DOM release and transformation differently, depending on the plastic composition. This highlights the significance of exploring MPs-DOM transformation in securing environmental safety.

Protein corona-induced extraction coupled to Fenton oxidation for selective and non-destructive preconcentration of Ag2S nanoparticles from waters.

Sulfidation of silver nanoparticles (AgNPs) to generate silver sulfide nanoparticles (Ag2S-NPs) significantly influences their fate and toxicity in natural environments. However, the correlational research in this field was limited by the lack of methods for speciation analysis of Ag2S-NPs. To address this challenge, a novel protocol for the selective Ag2S-NP extraction from real waters was developed using protein corona-induced extraction coupled to Fenton oxidation of AgNPs with Fe3+/H2O2 reagents. The ability of various concentrations of Fe3+/H2O2 to selectively dissociate AgNPs into ions was first evaluated. Then, selective separation and preconcentration of the remaining Ag2S-NPs was established by optimizing the parameters that may affect the protein corona-induced extraction efficiency, followed by quantification with inductively coupled plasma mass spectrometry (ICP-MS), enabling an ultrahigh enrichment factor of 10,000 and extremely low detection limit (LOD) of 1.8 ng/L. The presence of humic acid (HA), inorganic salts and particles at the environmentally relevant levels had limited effects on Ag2S-NP extraction. As demonstrated by transmission electron microscopy (TEM) analysis and single particle ICP-MS (spICP-MS), the sizes, shapes, and compositions of Ag2S-NPs extracted with the proposed method remain in intact. Good recoveries of 83.7-105% were achieved for the Ag2S-NPs spiked in four natural waters at the level of 97.8 ng/L. Due to the high yields and applicability to Ag2S-NPs at environmentally relevant concentrations, this proposed method is particularly suitable to track the generation and transformation of Ag2S-NPs in various scenarios.