RESUMEN
Klenow fragment of Escherichia coli DNA polymerase I, which was cocrystallized with duplex DNA, positioned 11 base pairs of DNA in a groove that lies at right angles to the cleft that contains the polymerase active site and is adjacent to the 3' to 5' exonuclease domain. When the fragment bound DNA, a region previously referred to as the "disordered domain" became more ordered and moved along with two helices toward the 3' to 5' exonuclease domain to form the binding groove. A single-stranded, 3' extension of three nucleotides bound to the 3' to 5' exonuclease active site. Although this cocrystal structure appears to be an editing complex, it suggests that the primer strand approaches the catalytic site of the polymerase from the direction of the 3' to 5' exonuclease domain and that the duplex DNA product may bend to enter the cleft that contains the polymerase catalytic site.
Asunto(s)
ADN Polimerasa I/química , ADN/metabolismo , Escherichia coli/enzimología , Secuencia de Bases , Sitios de Unión , Cristalización , ADN/química , ADN Polimerasa I/metabolismo , Replicación del ADN , ADN de Cadena Simple/química , ADN de Cadena Simple/metabolismo , Modelos Moleculares , Datos de Secuencia Molecular , Conformación Proteica , Moldes GenéticosRESUMEN
Site-directed mutagenesis of the large fragment of DNA polymerase I (Klenow fragment) yielded two mutant proteins lacking 3',5'-exonuclease activity but having normal polymerase activity. Crystallographic analysis of the mutant proteins showed that neither had any alteration in protein structure other than the expected changes at the mutation sites. These results confirmed the presumed location of the exonuclease active site on the small domain of Klenow fragment and its physical separation from the polymerase active site. An anomalous scattering difference Fourier of a complex of the wild-type enzyme with divalent manganese ion and deoxythymidine monophosphate showed that the exonuclease active site has binding sites for two divalent metal ions. The properties of the mutant proteins suggest that one metal ion plays a role in substrate binding while the other is involved in catalysis of the exonuclease reaction.
Asunto(s)
ADN Polimerasa I , Sitios de Unión , Catálisis , Gráficos por Computador , Cristalografía , Análisis Mutacional de ADN , ADN Polimerasa I/genética , Escherichia coli/enzimología , Exonucleasas , Metales , Modelos Moleculares , Conformación Proteica , Relación Estructura-ActividadRESUMEN
A self-cleaving element for use in bioseparations has been derived from a naturally occurring, 43 kDa protein splicing element (intein) through a combination of protein engineering and random mutagenesis. A mini-intein (18 kDa) previously engineered for reduced size had compromised activity and was therefore subjected to random mutagenesis and genetic selection. In one selection a mini-intein was isolated with restored splicing activity, while in another, a mutant was isolated with enhanced, pH-sensitive C-terminal cleavage activity. The enhanced-cleavage mutant has utility in affinity fusion-based protein purification. These mutants also provide new insights into the structural and functional roles of some conserved residues in protein splicing.
Asunto(s)
Mutagénesis , Ingeniería de Proteínas/métodos , Precursores de Proteínas/metabolismo , Empalme de Proteína/genética , Proteínas Recombinantes/aislamiento & purificación , Secuencia de Aminoácidos , Cromatografía de Afinidad , Girasa de ADN , ADN-Topoisomerasas de Tipo II/genética , ADN-Topoisomerasas de Tipo II/aislamiento & purificación , Endopeptidasas/genética , Endopeptidasas/metabolismo , Leucina/genética , Modelos Moleculares , Datos de Secuencia Molecular , Mycobacterium/genética , Rec A Recombinasas/genética , Timidilato Sintasa/genética , Timidilato Sintasa/aislamiento & purificación , Valina/genéticaRESUMEN
Toward the goal of identifying complete sets of transcription factor (TF)-binding sites in the genomes of several gamma proteobacteria, and hence describing their transcription regulatory networks, we present a phylogenetic footprinting method for identifying these sites. Probable transcription regulatory sites upstream of Escherichia coli genes were identified by cross-species comparison using an extended Gibbs sampling algorithm. Close examination of a study set of 184 genes with documented transcription regulatory sites revealed that when orthologous data were available from at least two other gamma proteobacterial species, 81% of our predictions corresponded with the documented sites, and 67% corresponded when data from only one other species were available. That the remaining predictions included bona fide TF-binding sites was proven by affinity purification of a putative transcription factor (YijC) bound to such a site upstream of the fabA gene. Predicted regulatory sites for 2097 E.coli genes are available at http://www.wadsworth.org/resnres/bioinfo/.
Asunto(s)
Sitios de Unión/genética , Gammaproteobacteria/genética , Genoma Bacteriano , Filogenia , Secuencia de Bases , ADN Bacteriano/genética , ADN Bacteriano/metabolismo , Bases de Datos Factuales , Escherichia coli/genética , Genes Bacterianos/genética , Unión Proteica , Alineación de Secuencia , Homología de Secuencia de Ácido Nucleico , Factores de Transcripción/metabolismoRESUMEN
I-TevI, the T4 td intron-encoded endonuclease, catalyzes the first step in intron homing by making a double-strand break in the intronless allele within a sequence designated the homing site. The 28 kDa enzyme, which interacts with the homing site over a span of 37 bp, binds as a monomer, contacting two domains of the substrate. In this study, limited proteolysis experiments indicate that I-TevI consists of two domains that behave as discrete physical entities as judged by a number of functional and structural criteria. Overexpression clones for each domain were constructed and the proteins were purified. The carboxy-terminal domain has DNA-binding activity coincident with the primary binding region of the homing site and binds with the same affinity as the full-length enzyme. The isolated amino-terminal domain, contains the conserved GIY-YIG motif, consistent with its being the catalytic domain. Furthermore, site-directed mutagenesis of a conserved arginine residue within the extended motif rendered the full-length protein catalytically inactive, although DNA-binding was maintained. This is the first evidence that the GIY-YIG motif is important for catalytic activity. An enzyme with an N-terminal catalytic domain and a C-terminal DNA-binding domain connected by a flexible linker is in accord with the bipartite structure of the homing site.
Asunto(s)
Endodesoxirribonucleasas/química , Endodesoxirribonucleasas/genética , Intrones , Secuencia de Aminoácidos , Bacteriófago T4/genética , Bacteriófago T4/metabolismo , Secuencia de Bases , Sitios de Unión , Huella de ADN , ADN Viral/química , ADN Viral/genética , ADN Viral/metabolismo , Endodesoxirribonucleasas/metabolismo , Cinética , Datos de Secuencia Molecular , Estructura Molecular , Mutagénesis Sitio-Dirigida , Conformación ProteicaRESUMEN
In order to improve our understanding of the 5'-3' exonuclease reaction catalyzed by Escherichia coli DNA polymerase I, we have constructed expression plasmids and developed purification methods for whole DNA polymerase I and its 5'-3' exonuclease domain that allow the production of large quantities of highly purified material suitable for biophysical and other studies. We have studied the enzymatic properties of the 5'-3' exonuclease, both as an isolated domain and in the context of the whole polymerase, using a variety of model oligonucleotides to explore the enzyme-substrate interaction. The 5'-3' exonuclease is known to be a structure-specific nuclease that cleaves a 5' displaced strand at the junction between single-stranded and duplex regions. Since the isolated domain shows the same structure specificity as the whole polymerase, the correct geometry of substrate binding is achieved without the assistance of the polymerase domain. The 5'-3' exonuclease reaction has a strict requirement for a free 5' end on the displaced strand; however, the upstream template and primer strands are dispensable. Site-directed mutagenesis of the ten carboxylate residues that are highly conserved among bacterial and bacteriophage 5'-3' exonucleases indicates that nine of them are important in the reaction. This finding is discussed in relation to structural and mutational data for related 5' nucleases.
Asunto(s)
ADN Polimerasa I/química , Secuencia de Aminoácidos , Ácidos Carboxílicos , Secuencia de Consenso , Análisis Mutacional de ADN , ADN Polimerasa I/genética , Escherichia coli/enzimología , Exonucleasas/química , Exonucleasas/genética , Datos de Secuencia Molecular , Alineación de Secuencia , Homología de Secuencia de Aminoácido , Relación Estructura-Actividad , Especificidad por SustratoRESUMEN
To reduce the number of recovery steps during downstream processing and to overcome the limitations of present fusion-based affinity separations, a controllable self-splicing protein element in the form of a mini-intein was used to optimize the recovery of proteins for both batch and flow purification strategies. The ability to recover purified proteins was demonstrated using a tripartite fusion consisting of a maltose binding domain, a truncated intein as a controllable linker molecule, and a protein of interest. To characterize expression level, solubility, cleavage rates, pH and temperature controllability, and protein activity, recombinant human acidic fibroblast growth factor (aFGF) was used as a model protein. A simple mass transport model, based on cleavage reaction-limited mass transfer and constant dispersion, was successfully used to predict product concentration and peak shape in relation to critical process parameters (with no fitting parameters). Insight into the nature of the cleavage reaction and its regulation was obtained via temperature- and pH-dependent kinetic data.
Asunto(s)
Cromatografía de Afinidad/métodos , Factor 1 de Crecimiento de Fibroblastos/aislamiento & purificación , Electroforesis en Gel de Poliacrilamida , Factor 1 de Crecimiento de Fibroblastos/química , Humanos , Concentración de Iones de Hidrógeno , Hidrólisis , Modelos Químicos , TemperaturaAsunto(s)
ADN Polimerasa I/aislamiento & purificación , Escherichia coli/enzimología , Fragmentos de Péptidos/aislamiento & purificación , Cromatografía en Gel/métodos , Cromatografía por Intercambio Iónico/métodos , Clonación Molecular , ADN Polimerasa I/biosíntesis , ADN Polimerasa I/metabolismo , Inducción Enzimática , Indicadores y Reactivos , Ácido Nalidíxico/farmacología , Fragmentos de Péptidos/biosíntesis , Fragmentos de Péptidos/metabolismo , Plásmidos , Proteínas Recombinantes/biosíntesis , Proteínas Recombinantes/aislamiento & purificación , Proteínas Recombinantes/metabolismoAsunto(s)
ADN Polimerasa I/química , ADN Polimerasa I/metabolismo , ADN Polimerasa Dirigida por ADN/química , ADN Polimerasa Dirigida por ADN/metabolismo , Exodesoxirribonucleasas/química , Exodesoxirribonucleasas/metabolismo , Secuencia de Aminoácidos , Sitios de Unión , Cationes Bivalentes/metabolismo , Clonación Molecular , Nucleótidos de Desoxiadenina/metabolismo , Exodesoxirribonucleasa V , Enlace de Hidrógeno , Cinética , Modelos Moleculares , Mutagénesis Sitio-Dirigida , Radioisótopos de Fósforo , Mutación Puntual , Conformación Proteica , Técnica de Dilución de Radioisótopos , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Especificidad por SustratoRESUMEN
We describe experiments to produce large quantities of the polymerase domain of E. coli DNA polymerase I for biochemical and biophysical studies. The polymerase domain derivative used in previous studies was insoluble when overproduced and tended to aggregate during purification. These problems were solved by a combination of two distinct strategies. By changing the expression system, we were able to obtain the overproduced protein in a soluble form, a necessary first step since attempts to purify the polymerase domain from the insoluble pellet were unsuccessful. The tendency of the polymerase domain to aggregate was eliminated by re-engineering the protein so as to remove both a solvent-exposed hydrophobic patch and a potentially unstructured region at the extreme N-terminus. Unlike the original construct, the re-engineered derivatives chromatographed as a single species and could be purified to homogeneity in good yield. Our experience in this study emphasizes the level of ignorance of the factors that influence protein overproduction and the need, in difficult cases, to evaluate many strategies in a semi-empirical manner.
Asunto(s)
ADN Polimerasa I/química , Secuencia de Aminoácidos , Secuencia de Bases , ADN Polimerasa I/metabolismo , Escherichia coli/enzimología , Expresión Génica , Ingeniería Genética , Datos de Secuencia Molecular , Mutagénesis Sitio-Dirigida , Regiones Promotoras Genéticas , Estructura Terciaria de Proteína , Proteínas Recombinantes/química , Proteínas Recombinantes/aislamiento & purificación , Proteínas Recombinantes/metabolismo , Solubilidad , Relación Estructura-ActividadRESUMEN
We have used site-directed mutagenesis to change amino acid side chains that have been shown crystallographically to be in close proximity to a DNA 3' terminus bound at the 3'-5' exonuclease active site of Klenow fragment. Exonuclease assays of the resulting mutant proteins indicate that the largest effects on exonuclease activity result from mutations in a group of carboxylate side chains (Asp355, Asp424 and Asp501) anchoring two divalent metal ions that are essential for exonuclease activity. Another carboxylate (Glu357) within this cluster seems to be less important as a metal ligand, but may play a separate role in catalysis of the exonuclease reaction. A second group of residues (Leu361, Phe473 and Tyr497), located around the terminal base and ribose positions, plays a secondary role, ensuring correct positioning of the substrate in the active site and perhaps also facilitating melting of a duplex DNA substrate by interacting with the frayed 3' terminus. The pH-dependence of the 3'-5' exonuclease reaction is consistent with a mechanism in which nucleophilic attack on the terminal phosphodiester bond is initiated by a hydroxide ion coordinated to one of the enzyme-bound metal ions.
Asunto(s)
ADN Polimerasa I/metabolismo , Escherichia coli/enzimología , Exodesoxirribonucleasas/metabolismo , Secuencia de Aminoácidos , Sitios de Unión , ADN Polimerasa I/genética , Exodesoxirribonucleasa V , Exodesoxirribonucleasas/genética , Cinética , Modelos Moleculares , Mutagénesis Sitio-Dirigida , Fragmentos de Péptidos/metabolismo , Conformación ProteicaRESUMEN
An Escherichia coli gene, stpA, has been identified and cloned based on its ability to suppress the Td- phenotype of a resident, splicing-defective phage T4 td (thymidylate synthase) gene. The stpA gene, which was localized to 60.24 min on the E. coli chromosome, encodes a 15.3-kDa protein. Overproduction of StpA in vivo led to an increase in td pre-mRNA levels and modest enhancement of td mRNA:pre-mRNA ratios. Consistent with its in vivo effect, purified StpA promoted RNA splicing in vitro, and facilitated RNA annealing and strand exchange with model substrates. These results suggest that StpA promotes splicing of the intron by binding RNA nonspecifically, resolving misfolded precursor molecules and facilitating association of critical base pair elements. Furthermore, proteinase K treatment of StpA-assembled precursors prior to the initiation of the splicing reaction still resulted in splicing enhancement, indicating that StpA is not required for the catalytic step, unlike the Neurospora splicing effector CYT-18, whose presence was necessary for catalysis to proceed. Together these results suggest that StpA has chaperone activity in vitro, with the property of promoting assembly of the precursors into an active conformation, in contrast to splicing effectors that stabilize the catalytically active intron structure.
Asunto(s)
Proteínas Bacterianas/genética , Proteínas de Unión al ADN , Proteínas de Escherichia coli , Escherichia coli/genética , Chaperonas Moleculares , Empalme del ARN , Secuencia de Aminoácidos , Secuencia de Bases , Proteínas Fúngicas/genética , Intrones , Modelos Genéticos , Datos de Secuencia Molecular , ARN/biosíntesis , Precursores del ARN/metabolismo , Timidilato Sintasa/genéticaRESUMEN
I-TevI is a site-specific, sequence-tolerant intron endonuclease. The crystal structure of the DNA-binding domain of I-TevI complexed with the 20 bp primary binding region of its DNA target reveals an unusually extended structure composed of three subdomains: a Zn finger, an elongated segment containing a minor groove-binding alpha-helix, and a helix-turn-helix. The protein wraps around the DNA, mostly following the minor groove, contacting the phosphate backbone along the full length of the duplex. Surprisingly, while the minor groove-binding helix and the helix-turn- helix subdomain make hydrophobic contacts, the few base-specific hydrogen bonds occur in segments that lack secondary structure and flank the intron insertion site. The multiple base-specific interactions over a long segment of the substrate are consistent with the observed high site specificity in spite of sequence tolerance, while the modular composition of the domain is pertinent to the evolution of homing endonucleases.
Asunto(s)
ADN/metabolismo , Endodesoxirribonucleasas/metabolismo , Secuencia de Bases , Endodesoxirribonucleasas/química , Evolución Molecular , Secuencias Hélice-Asa-Hélice , Enlace de Hidrógeno , Conformación Proteica , Especificidad por Sustrato , Dedos de ZincRESUMEN
Inteins are protein-splicing elements, most of which contain conserved sequence blocks that define a family of homing endonucleases. Like group I introns that encode such endonucleases, inteins are mobile genetic elements. Recent crystallography and computer modeling studies suggest that inteins consist of two structural domains that correspond to the endonuclease and the protein-splicing elements. To determine whether the bipartite structure of inteins is mirrored by the functional independence of the protein-splicing domain, the entire endonuclease component was deleted from the Mycobacterium tuberculosis recA intein. Guided by computer modeling studies, and taking advantage of genetic systems designed to monitor intein function, the 440-aa Mtu recA intein was reduced to a functional mini-intein of 137 aa. The accuracy of splicing of several mini-inteins was verified. This work not only substantiates structure predictions for intein function but also supports the hypothesis that, like group I introns, mobile inteins arose by an endonuclease gene invading a sequence encoding a small, functional splicing element.
Asunto(s)
Endonucleasas/genética , Evolución Molecular , Modelos Genéticos , Empalme de Proteína/genética , Secuencia de Aminoácidos , Fusión Artificial Génica , Simulación por Computador , Secuencia Conservada , Intrones , Mycobacterium tuberculosis/genética , Plásmidos , Rec A Recombinasas/biosíntesis , Rec A Recombinasas/genética , Eliminación de SecuenciaRESUMEN
I-TevI is a member of the GIY-YIG family of homing endonucleases. It is folded into two structural and functional domains, an N-terminal catalytic domain and a C-terminal DNA-binding domain, separated by a flexible linker. In this study we have used genetic analyses, computational sequence analysis andNMR spectroscopy to define the configuration of theN-terminal domain and its relationship to the flexible linker. The catalytic domain is an alpha/beta structure contained within the first 92 amino acids of the 245-amino acid protein followed by an unstructured linker. Remarkably, this structured domain corresponds precisely to the GIY-YIG module defined by sequence comparisons of 57 proteins including more than 30 newly reported members of the family. Although much of the unstructured linker is not essential for activity, residues 93-116 are required, raising the possibility that this region may adopt an alternate conformation upon DNA binding. Two invariant residues of the GIY-YIG module, Arg27 and Glu75, located in alpha-helices, have properties of catalytic residues. Furthermore, the GIY-YIG sequence elements for which the module is named form part of a three-stranded antiparallel beta-sheet that is important for I-TevI structure and function.