Temporal Dynamics of Bacterial and Fungal Colonization on Plastic Debris in the North Sea.

Despite growing evidence that biofilm formation on plastic debris in the marine environment may be essential for its biodegradation, the underlying processes have yet to be fully understood. Thus, far, bacterial biofilm formation had only been studied after short-term exposure or on floating plastic, yet a prominent share of plastic litter accumulates on the seafloor. In this study, we explored the taxonomic composition of bacterial and fungal communities on polyethylene plastic sheets and dolly ropes during long-term exposure on the seafloor, both at a harbor and an offshore location in the Belgian part of the North Sea. We reconstructed the sequence of events during biofilm formation on plastic in the harbor environment and identified a core bacteriome and subsets of bacterial indicator species for early, intermediate, and late stages of biofilm formation. Additionally, by implementing ITS2 metabarcoding on plastic debris, we identified and characterized for the first time fungal genera on plastic debris. Surprisingly, none of the plastics exposed to offshore conditions displayed the typical signature of a late stage biofilm, suggesting that biofilm formation is severely hampered in the natural environment where most plastic debris accumulates.

Bacterial Community Profiling of Plastic Litter in the Belgian Part of the North Sea.

Bacterial colonization of marine plastic litter (MPL) is known for over four decades. Still, only a few studies on the plastic colonization process and its influencing factors are reported. In this study, seafloor MPL was sampled at different locations across the Belgian part of the North Sea to study bacterial community structure using 16S metabarcoding. These marine plastic bacterial communities were compared with those of sediment and seawater, and resin pellets sampled on the beach, to investigate the origin and uniqueness of plastic bacterial communities. Plastics display great variation of bacterial community composition, while each showed significant differences from those of sediment and seawater, indicating that plastics represent a distinct environmental niche. Various environmental factors correlate with the diversity of MPL bacterial composition across plastics. In addition, intrinsic plastic-related factors such as pigment content may contribute to the differences in bacterial colonization. Furthermore, the differential abundance of known primary and secondary colonizers across the various plastics may indicate different stages of bacterial colonization, and may confound comparisons of free-floating plastics. Our studies provide insights in the factors that shape plastic bacterial colonization and shed light on the possible role of plastic as transport vehicle for bacteria through the aquatic environment.

A comprehensive assessment of plastic remediation technologies.

The global presence of plastic litter and its accumulation in the environment has become an issue of concern to the public and policymakers. This concern has triggered innovators in past decades to design and develop a multitude of remediation technologies to prevent plastic from entering the environment, or to clean up legacy litter. This study aims to (i) systematically review the current scientific literature on plastic remediation technologies, (ii) create a 'plastic clean-up and prevention overview' illustrating 124 remediation technologies and 29 characteristics, (iii) qualitatively analyse their key characteristics (e.g., fields of application, targeted plastic), and (iv) investigate challenges and opportunities of clean-up technologies for inland waterways (e.g., canals, rivers) and ports. We identified 61 scientific publications on plastic remediation technologies, until June 2022. Thirty-four of these studies were published within the last three years, demonstrating a growing interest. The presented overview indicates that inland waterways are, so far, the preferred field of application, with 22 technologies specifically designed for cleaning up plastics from inland waterways, and 52 additional ones with the potential to be installed in these locations. Given the importance of clean-up technologies in inland waterways, we highlighted their strengths, weaknesses, opportunities, and threats (SWOT). Our results indicate that, despite the challenges, these technologies provide essential prospects, from improving the environmental quality to raising awareness. Our study is instrumental as it illustrates an up-to-date overview and provides a comprehensive analysis of current in design phase, testing, and in use plastic remediation technologies.

A recipe for plastic: Expert insights on plastic additives in the marine environment.

The production and consumption of plastic products had been steadily increasing over the years, leading to more plastic waste entering the environment. Plastic pollution is ubiquitous and comes in many types and forms. To enhance or modify their properties, chemical additives are added to plastic items during manufacturing. The presence and leakage of these additives, from managed and mismanaged plastic waste, into the environment are of growing concern. In this study, we gauged, via an online questionnaire, expert knowledge on the use, characteristics, monitoring and risks of plastic additives to the marine environment. We analysed the survey results against actual data to identify and prioritise risks and gaps. Participants also highlighted key factors for future consideration, including gaining a deeper understanding of the use and types of plastic additives, how they leach throughout the entire lifecycle, their toxicity, and the safety of alternative options. More extensive chemical regulation and an evaluation of the essentiality of their use should also be considered.

Plastic pollution pathways from marine aquaculture practices and potential solutions for the North-East Atlantic region.

Aquaculture practices at sea are far from impact neutral and remain an important source of marine plastic pollution. With projected rapid continual growth in the sector, if left unmanaged, aquaculture pollution can have detrimental environmental and social implications. Using the DPSIR framework, the paper examines current practices and pathways of plastic pollution from marine aquaculture in the NE-Atlantic, drawing on findings from literature, stakeholder consultations and beach litter assessments. Pathways for aquaculture-related litter identified include rough weather, farmer behavior, inadequate access to recycling facilities, low price of consumable plastics and high cost of recycling. Beach litter analyses conducted as part of the study exposed serious issues of under quantification, resulting from difficulties in source identification and a lack of detailed categorization in official monitoring systems. The paper makes recommendations to improve litter quantification and waste management, including the use of local knowledge and experts to identify sources of marine litter.

Fragmentation of plastic objects in a laboratory seawater microcosm.

We studied the fragmentation of conventional thermoplastic and compostable plastic items in a laboratory seawater microcosm. In the microcosm, polyurethane foams, cellulose acetate cigarette filters, and compostable polyester and polylactic acid items readily sank, whereas polyethylene air pouches, latex balloons, polystyrene foams and polypropylene cups remained afloat. Microbial biofilms dominated by Cyanobacteria, Proteobacteria, Planctomycetes and Bacteriodetes grew on the plastics, and caused some of the polyethylene items to sink to the bottom. Electrical resistances (ER) of plastic items decreased as function of time, an indication that seawater had penetrated into microscopic crevices in the plastic that had developed over time. Rate constants for ER decrease in polyethylene items in the microcosm were similar to tensile elongation decrease of polyethylene sheets floating in sea, measured previously by others. Weight loss of plastic items was ≤ 1% per year for polyethylene, polystyrene and polypropylene, 3-5% for latex, polyethylene terephthalate and polyurethane, 15% for cellulose acetate, and 7-27% for polyester and polylactic acid compostable bags. The formation of microplastics observed in the microcosm was responsible for at least part of the weight loss. This study emphasizes the need to obtain experimental data on plastic litter degradation under conditions that are realistic for marine environments.

Reporting Guidelines to Increase the Reproducibility and Comparability of Research on Microplastics.

The ubiquitous pollution of the environment with microplastics, a diverse suite of contaminants, is of growing concern for science and currently receives considerable public, political, and academic attention. The potential impact of microplastics in the environment has prompted a great deal of research in recent years. Many diverse methods have been developed to answer different questions about microplastic pollution, from sources, transport, and fate in the environment, and about effects on humans and wildlife. These methods are often insufficiently described, making studies neither comparable nor reproducible. The proliferation of new microplastic investigations and cross-study syntheses to answer larger scale questions are hampered. This diverse group of 23 researchers think these issues can begin to be overcome through the adoption of a set of reporting guidelines. This collaboration was created using an open science framework that we detail for future use. Here, we suggest harmonized reporting guidelines for microplastic studies in environmental and laboratory settings through all steps of a typical study, including best practices for reporting materials, quality assurance/quality control, data, field sampling, sample preparation, microplastic identification, microplastic categorization, microplastic quantification, and considerations for toxicology studies. We developed three easy to use documents, a detailed document, a checklist, and a mind map, that can be used to reference the reporting guidelines quickly. We intend that these reporting guidelines support the annotation, dissemination, interpretation, reviewing, and synthesis of microplastic research. Through open access licensing (CC BY 4.0), these documents aim to increase the validity, reproducibility, and comparability of studies in this field for the benefit of the global community.

Bioaccumulation of PCBs from microplastics in Norway lobster (Nephrops norvegicus): An experimental study.

Plastic debris acts as a sorbent phase for hydrophobic organic compounds like polychlorinated biphenyls (PCBs). Chemical partitioning models predict that the ingestion of microplastics with adsorbed chemicals in the field will tend not to result in significant net desorption of the chemical to the organism's tissues. This is expected due to the often limited differences in fugacity of the chemical between the indigestible plastic materials and the tissues, which are typically already exposed in the same environment to the same chemicals as the plastic. However laboratory trials validating these model predictions are scarce. In this study, PCB-loaded microplastics were offered to field-collected Norway lobsters (Nephrops norvegicus) during in vivo feeding laboratory experiments. Each ingestion experiment was repeated with and without loading a mixture of ten PCB congeners onto plastic microspheres (MS) made of polyethylene (PE) and polystyrene (PS) with diameters of either 500-600 µm or 6 µm. We observed that the presence of chemicals adsorbed to ingested microplastics did not lead to significant bioaccumulation of the chemicals in the exposed organisms. There was a limited uptake of PCBs in Nephrops tail tissue after ingestion of PCB-loaded PE MS, while almost no PCBs were detected in animals exposed to PS MS. In general, our results demonstrated that after 3 weeks of exposure the ingestion of plastic MS themselves did not affect the nutritional state of wild Nephrops.

Microplastic contamination in brown shrimp (Crangon crangon, Linnaeus 1758) from coastal waters of the Southern North Sea and Channel area.

This study assessed the capability of Crangon crangon (L.), an ecologically and commercially important crustacean, of consuming plastics as an opportunistic feeder. We therefore determined the microplastic content of shrimp in shallow water habitats of the Channel area and Southern part of the North Sea. Synthetic fibers ranging from 200µm up to 1000µm size were detected in 63% of the assessed shrimp and an average value of 0.68±0.55microplastics/g w. w. (1.23±0.99microplastics/shrimp) was obtained for shrimp in the sampled area. The assessment revealed no spatial patterns in plastic ingestion, but temporal differences were reported. The microplastic uptake was significantly higher in October compared to March. The results suggest that microplastics >20µm are not able to translocate into the tissues.