RESUMEN
A quantitative GLC determination of 6-aminopenicillanic acid and 7-amino-3-methyl-delta3-cephem-4-carboxylic acid is presented. The results obtained are in good agreement with those of known chemical procedures. The method is free from interference by related substances.
Asunto(s)
Cefalosporinas/análisis , Ácido Penicilánico/análisis , Cromatografía de Gases , Compuestos de Trimetilsililo/análisis , Compuestos de Trimetilsililo/síntesis químicaRESUMEN
A rapid and accurate quantitative determination of cephacetrile in finished bulk and dosage forms is reported. The high-performance liquid chromatographic method is free of interference by acetyl hydrolysis products and synthesis by-products. The assay can be performed in about 15 min, affording less than 0.7% coefficients of variation within and between days. The chromatographic results are in good agreement with the microbiological assay requested by the "Code of Federal Regulations" for certification of cephacetrile sodium.