RESUMEN
INTRODUCTION: There are only a few reported cases of Salmonella enterica serotype Brandenburg foodborne outbreaks in the literature. In Italy Brandenburg is consistently present among the top ten serotypes from human source, but at low prevalences. MATERIALS AND METHODS: Fifty-five S. Brandenburg isolates from human, animal, environmental and food sources, including twelve isolates from a foodborne outbreak, were genotyped by Pulsed-Field Gel Electrophoresis (PFGE). RESULTS AND DISCUSSION: Eight pulsogroups and 19 pulsotypes were detected, with a unique pulsotype being attributed to the outbreak strains. Molecular subtyping can reliably complement the epidemiological investigations. Moreover, mapping molecular types of Salmonella isolates from human and non-human source may greatly contribute to risk assessment, by tracking possible animal sources, so improving cost-effectiveness of the prevention and control strategies.
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ADN Bacteriano/aislamiento & purificación , Brotes de Enfermedades/estadística & datos numéricos , Microbiología de Alimentos/métodos , Intoxicación Alimentaria por Salmonella/epidemiología , Intoxicación Alimentaria por Salmonella/microbiología , Salmonella enterica/aislamiento & purificación , Serotipificación/métodos , Adolescente , Adulto , Anciano , Animales , ADN Bacteriano/genética , Electroforesis en Gel de Campo Pulsado , Femenino , Humanos , Italia/epidemiología , Masculino , Persona de Mediana Edad , Vigilancia de la Población/métodos , Estudios Retrospectivos , Salmonella enterica/genética , Adulto JovenRESUMEN
Phenotypic and genetic characteristics of 21 strains of Salmonella serotype Heidelberg isolated in the years 1999-2003 from different sources in Italy were studied. Susceptibility patterns, plasmid analysis, and PFGE were used as epidemiological markers. Although non-homogeneous drug resistance patterns and plasmid profiles had been detected, PFGE patterns suggest the hypothesis of a nationwide clonal spread of this serotype associated with poultry.
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Infecciones por Salmonella/epidemiología , Infecciones por Salmonella/genética , Salmonella enterica/clasificación , Salmonella enterica/aislamiento & purificación , Animales , Pollos/microbiología , Niño , ADN Bacteriano/clasificación , ADN Bacteriano/genética , Farmacorresistencia Bacteriana Múltiple , Electroforesis en Gel de Campo Pulsado/métodos , Genotipo , Humanos , Italia/epidemiología , Masculino , Pruebas de Sensibilidad Microbiana/métodos , Epidemiología Molecular/clasificación , Epidemiología Molecular/métodos , Fenotipo , Vigilancia de la Población/métodos , Intoxicación Alimentaria por Salmonella/epidemiología , Infecciones por Salmonella/clasificación , Infecciones por Salmonella/metabolismo , Salmonella enterica/efectos de los fármacos , Salmonella enterica/genética , SerotipificaciónRESUMEN
Salmonella enterica subsp. enterica serovar Abortusovis is a major agent of abortion of the sheep and is firmly established, although at low prevalence, in Sicily. This paper describes the application rDNA gene restriction pattern fingerprinting to investigate relatedness among 7 serovar Abortusovis strains isolated at the "Istituto Zooprofilattico Sperimentale" of Sicily and 29 isolates identified at the Southern Italy Centre of Enterobacteriaceae between 1981 and 1989. Although Abortusovis serovar has exhibited a remarkable degree of homogeneity, genomic DNA polymorphisms, that have emerged, suggest possible importation of bacterial clones from different geographic areas.