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1.
BMC Gastroenterol ; 9: 99, 2009 Dec 30.
Artículo en Inglés | MEDLINE | ID: mdl-20040118

RESUMEN

BACKGROUND: It is frequently important to identify the prognosis of fulminant hepatic failure (FHF) patients as this will influence patient management and candidacy for liver transplantation. Therefore, a novel scoring system based on metabonomics combining with multivariate logistic regression was developed to predict the prognosis of FHF mouse model. METHODS: BALB/c mice were used to construct FHF model. Parts of plasma were collected at 4, 5, and 6-h time points after treatment, respectively, and detected using gas chromatography/time-of-flight mass spectrometry (GC/TOFMS). The acquired data were processed using partial least square discriminant analysis (PLS-DA). The metabolic markers identified were used to construct a scoring system by multivariate regression analysis. RESULTS: 28 mice of survival group and 28 of dead group were randomly selected and analyzed. PLS regression analysis showed that both the PLS models of 5 h and 6 h after d-galactosamine/lipopolysaccharide treatment demonstrated good performances. Loadings plot suggested that phosphate, beta-hydroxybutyrate (HB), urea, glucose and lactate concentrations in plasma had the highest weightings on the clustering differences at the three time points. By the multivariate logistic regression analysis, the death/survival index (DSI) was constructed based on relative concentrations of HB, urea and phosphate. It provided general accurate rate of prediction of 93.3% in the independent samples. CONCLUSIONS: The novel scoring system based on metabonomics combining with multivariate logistic regression is accurate in predicting the prognosis of FHF mouse model and may be referred in clinical practice as a more useful prognostic tool with other available information.


Asunto(s)
Fallo Hepático Agudo/sangre , Fallo Hepático Agudo/diagnóstico , Metabolómica/métodos , Modelos Estadísticos , Ácido 3-Hidroxibutírico/sangre , Animales , Biomarcadores/sangre , Modelos Animales de Enfermedad , Galactosamina/efectos adversos , Lipopolisacáridos/efectos adversos , Fallo Hepático Agudo/inducido químicamente , Modelos Logísticos , Masculino , Ratones , Ratones Endogámicos BALB C , Fosfatos/sangre , Valor Predictivo de las Pruebas , Pronóstico , Ribitol/sangre , Urea/sangre
2.
Nat Microbiol ; 3(5): 622-631, 2018 05.
Artículo en Inglés | MEDLINE | ID: mdl-29662128

RESUMEN

Insulin resistance is a risk factor for obesity and diabetes and predisposes individuals to Staphylococcus aureus colonization; however, the contribution of S. aureus to insulin resistance remains unclear. Here, we show that S. aureus infection causes impaired glucose tolerance via secretion of an insulin-binding protein extracellular domain of LtaS, eLtaS, which blocks insulin-mediated glucose uptake. Notably, eLtaS transgenic mice (eLtaS trans ) exhibited a metabolic syndrome similar to that observed in patients, including increased food and water consumption, impaired glucose tolerance and decreased hepatic glycogen synthesis. Furthermore, transgenic mice showed significant metabolic differences compared to their wild-type counterparts, particularly for the early insulin resistance marker α-hydroxybutyrate. We subsequently developed a full human monoclonal antibody against eLtaS that blocked the interaction between eLtaS and insulin, which effectively restored glucose tolerance in eLtaS trans and S. aureus-challenged mice. Thus, our results reveal a mechanism for S. aureus-induced insulin resistance.


Asunto(s)
Proteínas Bacterianas/química , Proteínas Bacterianas/genética , Resistencia a la Insulina , Insulina/metabolismo , Infecciones Estafilocócicas/complicaciones , Staphylococcus aureus/patogenicidad , Animales , Anticuerpos Monoclonales Humanizados/administración & dosificación , Anticuerpos Monoclonales Humanizados/farmacología , Proteínas Bacterianas/metabolismo , Sitios de Unión , Línea Celular , Femenino , Células Hep G2 , Humanos , Hidroxibutiratos/metabolismo , Síndrome Metabólico/tratamiento farmacológico , Síndrome Metabólico/genética , Síndrome Metabólico/metabolismo , Ratones , Ratones Transgénicos , Ratas , Infecciones Estafilocócicas/tratamiento farmacológico , Infecciones Estafilocócicas/metabolismo , Staphylococcus aureus/metabolismo
3.
Artículo en Zh | MEDLINE | ID: mdl-14761389

RESUMEN

OBJECTIVE: To investigate the relationship between the changes of amino acids contents in hippocampus of rats and electromagnetic pulse (EMP) exposure. METHODS: Rats were decapitated and hippocampus were removed after EMP (6 x 10(4) V/m, rise time 20 ns, pulse width 30 micro s, 5 pulses in 2 minutes) irradiation, and contents of amino acids were detected with high performance liquid chromatograpy (HPLC). RESULTS: The contents of aspartic acid (Asp) and glutamic acid (Glu) increased significantly 0, 3, 6 h after irradiation. The peak values of Asp [(17.25 +/- 1.63) pmol/ micro l] and Glu [(13.67 +/- 0.95) pmol/ micro l] were higher than those of control [(10.56 +/- 1.50), (6.94 +/- 1.10) pmol/ micro l respectively, P < 0.05]. Then both decreased gradually and reached the normal level 24 - 48 h after irradiation. The contents of glycine (Gly), taurine (Tau) and gamma-aminobutyric acid (GABA) also rose after exposure, the peak value of them [(4.51 +/- 0.60), (29.85 +/- 2.70), (5.14 +/- 0.73) pmol/ micro l respectively] were higher than those of control group [(2.18 +/- 0.31), (9.88 +/- 1.47), (2.84 +/- 0.67) pmol/ micro l, P < 0.05], then recovered 48 h after irradiation. The value of Glu/GABA increased immediately after exposure (3.45 +/- 0.25, P < 0.05), then decreased 24 h (1.62 +/- 0.23, P < 0.05) and recovered 48 h after exposure. CONCLUSION: The toxic effect of excess excitatory amino acids may be partly responsible for the early retardation (within 24 h) of learning of rats.


Asunto(s)
Aminoácidos/análisis , Hipocampo/efectos de la radiación , Radiación , Animales , Cromatografía Líquida de Alta Presión/métodos , Ácido Glutámico/análisis , Hipocampo/metabolismo , Masculino , Distribución Aleatoria , Ratas , Ratas Wistar , Factores de Tiempo
4.
Magn Reson Chem ; 45(9): 739-44, 2007 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-17638316

RESUMEN

A mixture of a pair of stereoisomeric new spirostanol saponins (1a and 1b) and a new cholestane saponin (2) were isolated from the rhizome of Paris pollyphylla Smith var. yunnanensis. Their structures were elucidated as (25R)-spirost-5-en-3beta, 7beta-diol-3-O-alpha-L-arabinofuranosyl-(1 --> 4)-[alpha-L-rhamnopyranosyl-(1 --> 2)]-beta-D-glucopyranoside (1a), (25R)-spirost-5-en-3beta, 7alpha-diol-3-O-alpha-L-arabinofuranosyl-(1 --> 4)-[alpha-L-rhamnopyranosyl-(1 --> 2)]-beta-D-glucopyranoside (1b) and 26-O-beta-D-glucopyranosyl-(25R)-Delta(5(6), 17(20))-dien-16, 22-dione-cholestan-3beta, 26-diol-3-O-alpha-L-arabinofuranosyl-(1 --> 4)-[alpha-L-rhamnopyranosyl-(1 --> 2)]-beta-D-glucopyranoside (2) by a combination of HR-ESI-MS, FAB-MS, 1D and 2D NMR techniques (including (1)H-NMR, (13)C-NMR, (1)H--(1)H COSY, HSQC, HMBC and NOESY).


Asunto(s)
Liliaceae/química , Raíces de Plantas/química , Saponinas/química , Esteroides/química , Cromatografía Líquida de Alta Presión , Indicadores y Reactivos , Espectroscopía de Resonancia Magnética , Espectrometría de Masa por Ionización de Electrospray
5.
Chem Pharm Bull (Tokyo) ; 54(7): 931-5, 2006 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-16819206

RESUMEN

Ten furostanol saponins were isolated as five pairs of 25R and 25S epimers from the fresh rhizomes of Polygonatum kingianum. Seven of them were identified as new compounds, (25S)-kingianoside D (2), (25S)-kingiano-side C (4), (25R,22)-hydroxylwattinoside C (5), kingianoside E (7), (25S)-kingianoside E (8), kingianoside F (9) and (25S)-kingianoside F (10), together with three known saponins, kingianoside C (1), kingianoside D (3), and 22-hydroxylwattinoside C (6). The structures of the new saponins were determinded by detailed analysis of their 1D and 2D NMR spectra, and by comparison of the spectral data with those reported.


Asunto(s)
Polygonatum/química , Rizoma/química , Saponinas/química , Esteroles/química , Secuencia de Carbohidratos , Espectroscopía de Resonancia Magnética , Datos de Secuencia Molecular , Estructura Molecular , Saponinas/aislamiento & purificación , Estereoisomerismo , Esteroles/aislamiento & purificación
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