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1.
Plant J ; 118(6): 1848-1863, 2024 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-38488203

RESUMEN

Noncoding and coding RNAs are key regulators of plant growth, development, and stress responses. To investigate the types of transcripts accumulated during the vegetative to reproductive transition and floral development in the Coffea arabica L., we sequenced small RNA libraries from eight developmental stages, up to anthesis. We combined these data with messenger RNA and PARE sequencing of two important development stages that marks the transition of an apparent latent to a rapid growth stage. In addition, we took advantage of multiple in silico tools to characterize genomic loci producing small RNAs such as phasiRNAs, miRNAs, and tRFs. Our differential and co-expression analysis showed that some types of small RNAs such as tRNAs, snoRNAs, snRNAs, and phasiRNAs preferentially accumulate in a stage-specific manner. Members of the miR482/miR2118 superfamily and their 21-nucleotide phasiRNAs originating from resistance genes show a robust co-expression pattern that is maintained across all the evaluated developmental stages. Finally, the majority of miRNAs accumulate in a family stage-specific manner, related to modulated hormonal responses and transcription factor expression.


Asunto(s)
Coffea , Flores , Regulación de la Expresión Génica de las Plantas , MicroARNs , ARN de Planta , Coffea/genética , Coffea/crecimiento & desarrollo , Flores/genética , Flores/crecimiento & desarrollo , ARN de Planta/genética , MicroARNs/genética , Tetraploidía
2.
Sci Rep ; 10(1): 9514, 2020 06 11.
Artículo en Inglés | MEDLINE | ID: mdl-32528037

RESUMEN

Bud necrosis (BN) is a common disorder that affects Vitis vinifera L. and reduces its potential yield. To minimize the losses caused by BN, the double pruning management was applied in Brazilian Southeast vineyards. In this management strategy plants are pruned at the winter to promote a vegetative cycle and then, at summer, to promote the reproductive cycle at optimal environmental conditions. To investigate the relationship of BN and the double pruning management RNA-seq libraries were sequenced from healthy and necrotic tissues at four different stages of the year. The comparison of differentially expressed genes in necrotic and non-necrotic tissues showed an enhanced expression of genes related to cell death possibly induced by endophytic microorganisms in the necrotic tissues. The de novo assembly, characterization and quantification of transcripts within the RNA-seq libraries showed that genes from the endophytic fungus Alternaria alternata, responsible for the production of toxic compounds were highly expressed under BN. Here we propose a model in which unfavorable conditions and reduced carbohydrate levels in buds can promote the switch from a biotrophic lifestyle to a necrotrophic lifestyle in the endophytic fungi, which seems to be involved in the development of BN.


Asunto(s)
Alternaria/fisiología , Endófitos/fisiología , Perfilación de la Expresión Génica , Enfermedades de las Plantas/genética , Enfermedades de las Plantas/microbiología , Vitis/genética , Vitis/microbiología , Necrosis/genética , Brotes de la Planta/genética , Brotes de la Planta/microbiología , Reproducción
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