RESUMEN
Sucrose synthase (SUS) is a key enzyme in plant metabolism, as it serves to cleave the photosynthetic end-product sucrose into UDP-glucose and fructose. SUS is generally assumed to be a tetrameric protein, but results in the present study suggest that SUS can form dimers as well as tetramers and that sucrose may be a regulatory factor for the oligomerization status of SUS. The oligomerization of SUS may also affect the cellular localization of the protein. We show that sucrose concentration modulates the ability of SUS1 to associate with F-actin in vitro and that calcium-dependent protein kinase-mediated phosphorylation of recombinant SUS1 at the Ser15 site is a negative regulator of its association with actin. Although high sucrose concentrations and hyperphosphorylation have been shown to promote SUS association with the plasma membrane, we show that the opposite is true for the SUS-actin association. We also show that SUS1 has a unique 28 residue coiled-coil domain that does not appear to play a role in oligomerization, but may prove to be significant in the future for interactions of SUS with other proteins. Collectively, these results highlight the multifaceted nature of SUS association with cellular structures.
Asunto(s)
Actinas/metabolismo , Glucosiltransferasas/metabolismo , Proteínas de Plantas/metabolismo , Sacarosa/farmacología , Cromatografía en Gel , Dimerización , Quinasa 2 de Adhesión Focal/metabolismo , Glucosiltransferasas/química , Glucosiltransferasas/genética , Immunoblotting , Luz , Fosforilación , Proteínas de Plantas/química , Proteínas de Plantas/genética , Unión Proteica/efectos de los fármacos , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Plantones/efectos de los fármacos , Plantones/genética , Plantones/metabolismo , Zea mays/efectos de los fármacos , Zea mays/genética , Zea mays/metabolismoRESUMEN
Sucrose (Suc) synthase (SUS) cleaves Suc to form UDP glucose and fructose, and exists in soluble and membrane-associated forms, with the latter proposed to channel UDP glucose to the cellulose-synthase complex on the plasma membrane of plant cells during synthesis of cellulose. However, the structural features responsible for membrane localization and the mechanisms regulating its dual intracellular localization are unknown. The maize (Zea mays) SUS1 isoform is likely to have the intrinsic ability to interact directly with membranes because we show: (1) partial membrane localization when expressed in Escherichia coli, and (2) binding to carbonate-stripped plant microsomes in vitro. We have undertaken mutational analyses (truncations and alanine substitutions) and in vitro microsome-binding assays with the SUS1 protein to define intrinsic membrane-binding regions and potential regulatory factors that could be provided by cellular microenvironment. The results suggest that two regions of SUS1 contribute to membrane affinity: (1) the amino-terminal noncatalytic domain, and (2) a region with sequence similarity to the C-terminal pleckstrin homology domain of human pleckstrin. Alanine substitutions within the pleckstrin homology-like domain of SUS1 reduced membrane association in E. coli and with plant microsomes in vitro without reducing enzymatic activity. Microsomal association of wild-type SUS1 displayed cooperativity with SUS1 protein concentration and was stimulated by both lowering the pH and adding Suc. These studies offer insight into the molecular level regulation of SUS1 localization and its participation in carbon partitioning in plants. Moreover, transgenics with active SUS mutants altered in membrane affinity may be of technological utility.
Asunto(s)
Membrana Celular/enzimología , Glucosiltransferasas/metabolismo , Zea mays/enzimología , Secuencia de Aminoácidos , Proteínas Sanguíneas/química , Glucosiltransferasas/química , Sistema de la Enzima Desramificadora del Glucógeno/análisis , Humanos , Concentración de Iones de Hidrógeno , Datos de Secuencia Molecular , Mutagénesis Sitio-Dirigida , Mutación , Fosfoproteínas/química , Estructura Terciaria de Proteína , Homología de Secuencia de Aminoácido , Sacarosa/metabolismo , Zea mays/química , Zea mays/genéticaRESUMEN
Although sucrose synthase (SUS) is widely appreciated for its role in plant metabolism and growth, very little is known about the contribution of each of the SUS isoforms to these processes. Using isoform-specific antibodies, we evaluated the three known isoforms individually at the protein level. SUS1 and SUS-SH1 proteins have been studied previously; however, SUS2 (previously known as SUS3) has only been studied at the transcript level. Using SUS2 isoform-specific antibodies, we determined that this isoform is present in several maize tissues. The intracellular localization of all SUS isoforms was studied by cellular fractionation of leaves and developing kernels. Interestingly, SUS1 and SUS-SH1 were associated with membranes while SUS2 was not. The lack of membrane-associated SUS2 indicates that it might have a unique role in cytoplasmic sucrose metabolism. Using co-immunoprecipitation with kernel extracts, it was also established that SUS2 exists predominantly as a hetero-oligomer with SUS1, while SUS-SH1 forms only homo-oligomers. Using sequence-specific and phospho-specific antibodies, we have established for the first time that SUS-SH1 is phosphorylated in vivo at the Ser10 site in kernels, similar to the SUS1 Ser15 site. In midveins, additional evidence suggests that SUS can be phosphorylated at a novel C-terminal threonine site. Together, these results show that the isoforms of SUS are important in both cytosolic and membrane-associated sucrose degradation, but that their unique attributes most probably impart isoform-specific functional roles.
Asunto(s)
Glucosiltransferasas/metabolismo , Hojas de la Planta/enzimología , Raíces de Plantas/enzimología , Brotes de la Planta/enzimología , Zea mays/enzimología , Membrana Celular/enzimología , Membrana Celular/genética , Citosol/enzimología , Regulación Enzimológica de la Expresión Génica/genética , Regulación de la Expresión Génica de las Plantas/genética , Genes de Plantas/genética , Glucosiltransferasas/genética , Isoenzimas/genética , Isoenzimas/metabolismo , Fosforilación , Hojas de la Planta/genética , Raíces de Plantas/genética , Brotes de la Planta/genética , Zea mays/genéticaRESUMEN
In many organisms, an increasing number of proteins seem to play two or more unrelated roles. Here we report that maize sucrose synthase (SUS) is distributed in organelles not involved in sucrose metabolism and may have novel roles beyond sucrose degradation. Bioinformatics analysis predicts that among the three maize SUS isoforms, SH1 protein has a putative mitochondrial targeting peptide (mTP). We validated this prediction by the immunodetection of SUS in mitochondria. Analysis with isoform-specific antisera revealed that both SH1 and SUS1 are represented in mitochondria, although the latter lacks a canonical mTP. The SUS2 isoform is not detectable in mitochondria, despite its presence in the cytosol. In maize primary roots, the mitochondrion-associated SUS (mtSUS; which includes SH1 and SUS1) is present mostly in the root tip, indicating tissue-specific regulation of SUS compartmentation. Unlike the glycolytic enzymes that occur attached to the outside of mitochondria, SH1 and SUS1 are intramitochondrial. The low abundance of SUS in mitochondria, its high Km value for sucrose, and the lack of sucrose in mitochondria suggest that mtSUS plays a non-sucrolytic role. Co-immunoprecipitation studies indicate that SUS interacts with the voltage-dependent anion channel in an isoform-specific and anoxia-enhanced manner and may be involved in the regulation of solute fluxes into and out of mitochondria. In several plant species, at least one of the SUS proteins possesses a putative mTP, indicating the conservation of the noncatalytic function across plant species. Taken together, these observations suggest that SUS has a novel noncatalytic function in plant cells.