Seasonality and toxin production of Pyrodinium bahamense in a Red Sea lagoon.

Harmful algal blooms of the dinoflagellate Pyrodinium bahamense have caused human and economic losses in the last decades. This study, for the first time, documents a bloom of P. bahamense in the Red Sea. The alga was recurrently present in a semi-enclosed lagoon throughout nearly 2 years of observations. The highest cell densities (104-105cellsL-1) were recorded from September to beginning of December at temperatures and salinities of ∼26-32°C and ∼41, respectively. The peak of the bloom was recorded mid-November, before a sharp decrease in cell numbers at the end of December. Minimum concentrations in summer were at ∼103cellsL-1. A saxitoxin ELISA immunoassay of cultures and water samples confirmed the toxicity of the strain found in the Red Sea. Moreover, a gene expression analysis of the saxitoxin gene domain SxtA4 showed that transcript production peaked at the culmination of the bloom, suggesting a relation between transcript production, sudden cells increment-decline, and environmental factors.

Placental expression of glycophosphatidylinositol (GPI)-anchored proteins in paroxysmal nocturnal haemoglobinuria.

Paroxysmal nocturnal haemoglobinuria (PNH) is a clonal stem cell disorder in which a defect of glycophosphatidylinositol (GPI)-anchored proteins leads to higher morbidity and mortality because of intravascular haemolysis, haemoglobinuria, pancytopenia and an increased frequency of thrombotic events. We report here the clinical features of a pregnant woman with PNH and present an immunhistochemical analysis of complement regulators, leukocyte activation markers and placental alkaline phosphatase (PALP) on syncytiotrophoblasts and inflammatory cells in her placenta. Placental tissue from normal deliveries served as controls. The patient had severe PNH with haemolysis, thrombosis episodes and signs of bone marrow failure. Placental syncytiotrophoblasts and villous cells of fetal origin in both normal placentas and the placenta from the PNH patient expressed PALP and the complement regulators CD46, CD55 and CD59. Additionally, CD11b-positive leukocytes of presumed maternal origin were negative for CD15 in the PNH placenta, while they stained positive within the villous space and in normal placentas. These findings show that fetally derived cells in the PNH placenta expressed GPI-linked molecules that are known to be of importance for a successful pregnancy outcome.

Toxicity of four potentially ichthyotoxic marine phytoflagellates determined by four different test methods.

The toxicity of the marine phytoflagellates Prymnesium parvum. Prymnesium patelliferum, Chrysochromulina polylepis, and Chrysochromulina leadbeateri isolated from ichthyotoxic blooms in Norwegian coastal waters was compared using four different test methods developed for the detection of toxins produced by these species. The test methods were (1) lethality to the crustacean Artemia salina exposed to living algae, (2) hemolytic activity (lysis of human erythrocytes) by crude algal lipid extracts, and inhibition of the uptake of the neurotransmitters L-glutamate and gamma-aminobutyric acid (GABA) into (3) synaptosomes and (4) synaptic vesicles of rat brain by crude algal lipid extracts. All test methods indicated different levels of toxicity among the algal species. Prymnesium parvum, P. patelliferum, and C. polylepis were toxic as determined by all four test methods. The cultured strain of C. leadbeateri, although isolated from a toxic algal bloom, appeared nontoxic by the methods used here, and served as a negative control. The hemolytic activity of P. parvum extract was about nine times higher than that of P. patelliferum extract, whereas the activity was only two to three times higher using the other three methods. Chrysochromulina polylepis had higher toxic activity than P. patelliferum except for less inhibitory effect on synaptosomes. The inhibition of synaptosomal and vesicular neurotransmitter uptake systems by extracts of P. parvum, P. patelliferum, and C. polylepis appeared to be due to similar mechanisms of action, since similar inhibition ratios between the uptake of L-glutamate and GABA were obtained in both synaptosomes and synaptic vesicles. We suggest that P. parvum, P. patelliferum, and C. polylepis produce a set of similar toxins and that the relative amounts of each toxin vary among the three species.

Distribution of morphine and meperidine after intrathecal administration in rat and mouse.

Morphine and meperidine distribution in the neuroaxis were studied in rats after intrathecal injection through catheters ending at the lumbar level. 14C-Morphine and 3H-meperidine were injected with pharmacologic doses of each drug. Radioactivity was measured in spinal cord segments at different times. At 14 min the segment with maximum morphine concentration (T11-12) contained 8.6 +/- 2.4 (mean +/- SD) pmol/mg, a value 215 times higher than would be observed if distribution in the body were homogeneous. The ratio between concentration in the most rostral segment (C3-4) and in the segment with maximal concentration was 0.21 +/- 0.10. At 14 min the segment with maximum meperidine concentration (T9-10) contained 161.4 +/- 33.9 pmol/mg wet tissue, a value 75 times higher than would be seen with even distribution in the body. The ratio (C3-4 vs. T9-10) was 0.10 +/- 0.04 at this time. The distribution of 14C-morphine in the whole central nervous system (CNS) was studied in mice by whole body autoradiography after intrathecal injections of 5 microliters at the L5-6 level. High levels of radioactivity were detected in the whole spinal cord and in brain regions close to the basal cisterns until 2 h after injection. At 4 h only the caudal part of the spinal cord had detectable levels of radioactivity. The per cent of the injected dose of morphine that was recovered from the spinal cord was 26.5 +/- 4.5 at 14 min, 19.9 +/- 8.8 at 44 min, and 4.5 +/- 1.7 at 179 min after injection.(ABSTRACT TRUNCATED AT 250 WORDS)

Diagnostic and biological significance of anti-p41 IgM antibodies against Borrelia burgdorferi.

We performed a prospective study to investigate the biological significance and diagnostic specificity of anti-p41 immunoglobulin (Ig)M antibodies against Borrelia burgdorferi. During a 1-year interval 2403 patients were referred to our department for B. burgdorferi serology. Sixty-three patients had repetitive positive tests for IgM anti-p41 antibodies and negative tests for anti-p41 IgG antibodies. Ten of the 63 patients recently had symptoms of erythema migrans. A confirmatory IgM Western blot gave a positive reaction in 5 patients out of 53 patients with little or no clinical evidence of B. burgdorferi infection. The remaining 48 patients were negative in this test and were considered as false-positives. Two whole cell enzyme-linked immunosorbent assay (ELISAs), two immunofluorescence assays and Western blotting were not useful as confirmatory tests. Sera from 330 blood donors and 72 cord sera were also screened for anti-p41 IgM. Five blood donor sera and five cord sera showed an IgM reactivity against p41. Based on our data we hypothesize that up to 1.5% of the population may have natural IgM antibodies against p41 in their sera. We observed that six out of nine sera with such antibodies could immobilize a B. afzelii reference strain in vitro. Whether anti-p41 IgM antibodies are capable of inactivating infective spirochetes and thereby prevent infection in vivo is, however, not yet clarified. The paradoxical conclusion that anti-p41 IgM antibodies may be a sign of resistance to infection rather than a sign of infection should be given consideration.