RESUMEN
The Bub1 kinase is a critical component of the spindle checkpoint involved in monitoring the separation of sister chromatids at mitosis. The viral oncoprotein Simian virus 40 large T antigen (LT) can bind and perturb the spindle checkpoint function of Bub1. We have developed three highly specific monoclonal antibodies against the Bub1 protein and have demonstrated that they can all detect Bub1 via Western blotting and immunofluorescence, in addition to their ability to immunoprecipitate Bub1.
Asunto(s)
Anticuerpos Monoclonales/biosíntesis , Proteínas de Ciclo Celular/inmunología , Proteínas Quinasas/inmunología , Secuencias de Aminoácidos , Animales , Especificidad de Anticuerpos , Antígenos Virales de Tumores/inmunología , Proteínas de Ciclo Celular/química , Proteínas de Ciclo Celular/genética , Línea Celular , Hibridomas/inmunología , Ratones , Microscopía Fluorescente , Células 3T3 NIH , Proteínas Quinasas/química , Proteínas Quinasas/genética , Proteínas Serina-Treonina Quinasas , Proteínas Recombinantes de Fusión/química , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/inmunologíaRESUMEN
PDGF is a potent chemotactic mitogen and a strong inductor of fibroblast motility. In Swiss 3T3 fibroblasts, exposure to PDGF but not EGF or IGF-1 causes a rapid loss of actin stress fibers (SFs) and focal adhesions (FAs), which is followed by the development of retractile dendritic protrusions and induction of motility. The PDGF-specific actin reorganization was blocked by inhibition of Src-kinase and the 26S proteasome. PDGF induced Src-dependent association between the multifunctional transcription/translation regulator hnRNP-K and the mRNA-encoding myosin regulatory light-chain (MRLC)-interacting protein (MIR), a E(3)-ubiquitin ligase that is MRLC specific. This in turn rapidly increased MIR expression, and led to ubiquitination and proteasome-mediated degradation of MRLC. Downregulation of MIR by RNA muting prevented the reorganization of actin structures and severely reduced the migratory and wound-healing potential of PDGF-treated cells. The results show that activation of MIR and the resulting removal of diphosphorylated MRLC are essential for PDGF to instigate and maintain control over the actin-myosin-based contractile system in Swiss 3T3 fibroblasts. The PDGF induced protein destabilization through the regulation of hnRNP-K controlled ubiquitin -ligase translation identifies a novel pathway by which external stimuli can regulate phenotypic development through rapid, organelle-specific changes in the activity and stability of cytoskeletal regulators.
Asunto(s)
Actinas/efectos de los fármacos , Citoesqueleto/efectos de los fármacos , Ribonucleoproteína Heterogénea-Nuclear Grupo K/metabolismo , Factor de Crecimiento Derivado de Plaquetas/farmacología , Ubiquitina-Proteína Ligasas/metabolismo , Citoesqueleto de Actina/efectos de los fármacos , Citoesqueleto de Actina/metabolismo , Actinas/metabolismo , Animales , Movimiento Celular , Citoesqueleto/metabolismo , Citoesqueleto/ultraestructura , Regulación hacia Abajo , Activación Enzimática , Factor de Crecimiento Epidérmico/farmacología , Fibroblastos/efectos de los fármacos , Fibroblastos/fisiología , Fibroblastos/ultraestructura , Factor I del Crecimiento Similar a la Insulina/farmacología , Ratones , Cadenas Ligeras de Miosina/metabolismo , Complejo de la Endopetidasa Proteasomal , Inhibidores de Proteasoma , ARN Interferente Pequeño/genética , ARN Interferente Pequeño/farmacología , Células 3T3 Swiss , Ubiquitina-Proteína Ligasas/antagonistas & inhibidores , Ubiquitina-Proteína Ligasas/genética , Familia-src Quinasas/antagonistas & inhibidoresRESUMEN
The dynamic processes of cell migration and invasion are largely coordinated by Rho family GTPases. The scaffolding protein IQGAP1 binds to Cdc42, increasing the amount of active Cdc42 both in vitro and in cells. Here we show that overexpression of IQGAP1 in mammalian cells enhances cell migration in a Cdc42- and Rac1-dependent manner. Importantly, cell motility was significantly decreased both by knock down of endogenous IQGAP1 using small interfering RNA and by transfection of a dominant negative IQGAP1 construct, IQGAP1DeltaGRD. Cell invasion was similarly altered by manipulating intracellular IQGAP1 concentrations. Moreover, invasion mediated by constitutively active Cdc42 was attenuated by IQGAP1DeltaGRD. Thus, IQGAP1 has a fundamental role in cell motility and invasion.
Asunto(s)
Proteínas Portadoras/fisiología , Proteínas Activadoras de ras GTPasa , Animales , Western Blotting , Línea Celular Tumoral , Movimiento Celular , Genes Dominantes , Glutatión Transferasa/metabolismo , Humanos , Inmunohistoquímica , Ratones , Microscopía Confocal , Células 3T3 NIH , Invasividad Neoplásica , Plásmidos/metabolismo , ARN Interferente Pequeño/metabolismo , Factores de Tiempo , Transfección , Cicatrización de Heridas , Proteína de Unión al GTP cdc42/metabolismo , Proteína de Unión al GTP rac1/metabolismoRESUMEN
Integrins have been shown to exert regulatory influences on mammary differentiation and morphogenesis in rodent experimental systems. We have, therefore, examined the expression patterns of integrin subunits on human breast tissues obtained at the 12th, 15th and 18th weeks of pregnancy. Myoepithelial cells were positive for all the integrin subunits examined. alpha2, alpha6 and beta4 showed increased and more defined labelling during pregnancy, indicating that myoepithelial cells and extracellular matrix strengthen their support for the expanding alveoli during pregnancy. Sub-populations of stromal cells were positive for alpha1, alpha3, alpha6, beta1 and beta4. On luminal epithelial cells, alpha1, alpha2, alpha3, alpha6 and beta1 were detectable. alpha2 showed a focal decrease, but the expression patterns of other integrins in luminal cells did not change during pregnancy. Therefore, the expression patterns of most integrins existing prior to pregnancy seem sufficient in this cell type to support the morphological and functional development during early pregnancy.
Asunto(s)
Mama/crecimiento & desarrollo , Mama/metabolismo , Células Epiteliales/metabolismo , Matriz Extracelular/metabolismo , Integrinas/metabolismo , Embarazo/metabolismo , Células del Estroma/metabolismo , Adulto , Mama/citología , Diferenciación Celular/fisiología , Células Epiteliales/citología , Matriz Extracelular/ultraestructura , Femenino , Regulación del Desarrollo de la Expresión Génica/fisiología , Humanos , Inmunohistoquímica , Isoformas de Proteínas/metabolismo , Células del Estroma/citología , Regulación hacia Arriba/fisiologíaRESUMEN
Chronic myeloid leukemia (CML) is characterized by expression of the BCR-ABL fusion gene that encodes a 210-kDa protein, which is a constitutively active tyrosine kinase. At least 70% of the oncoprotein is localized to the cytoskeleton, and several of the most prominent tyrosine kinase substrates for p210(BCR-ABL) are cytoskeletal proteins. Dendritic cells (DCs) are bone marrow-derived antigen-presenting cells responsible for the initiation of immune responses. In CML patients, up to 98% of myeloid DCs generated from peripheral blood mononuclear cells are BCR-ABL positive. In this study we have compared the morphology and behavior of myeloid DCs derived from CML patients with control DCs from healthy individuals. We show that the actin cytoskeleton and shape of CML-DCs of myeloid origin adherent to fibronectin differ significantly from those of normal DCs. CML-DCs are also defective in processing and presentation of exogenous antigens such as tetanus toxoid. The antigen-processing defect may be a consequence of the reduced capacity of CML-DCs to capture antigen via macropinocytosis or via mannose receptors when compared with DCs generated from healthy individuals. Furthermore, chemokine-induced migration of CML-DCs in vitro was significantly reduced. These observations cannot be explained by a difference in the maturation status of CML and normal DCs, because phenotypic analysis by flow cytometry showed a similar surface expression of maturation makers. Taken together, these results suggest that the defects in antigen processing and migration we have observed in CML-DCs may be related to underlying cytoskeletal changes induced by the p210(BCR-ABL) fusion protein.
Asunto(s)
Citoesqueleto de Actina/ultraestructura , Actinas/análisis , Presentación de Antígeno , Citoesqueleto/ultraestructura , Células Dendríticas/patología , Lectinas Tipo C , Leucemia Mielógena Crónica BCR-ABL Positiva/inmunología , Lectinas de Unión a Manosa , Adolescente , Adulto , Adhesión Celular , Células Cultivadas/efectos de los fármacos , Células Cultivadas/inmunología , Células Cultivadas/patología , Quimiotaxis , Células Dendríticas/inmunología , Endocitosis , Fibronectinas , Humanos , Hibridación Fluorescente in Situ , Leucemia Mielógena Crónica BCR-ABL Positiva/patología , Activación de Linfocitos , Receptor de Manosa , Microscopía Confocal , Monocitos/efectos de los fármacos , Proteínas de Neoplasias/fisiología , Pinocitosis , Receptores de Superficie Celular/fisiología , Subgrupos de Linfocitos T/inmunología , Toxoide Tetánico/inmunologíaRESUMEN
The mitotic spindle checkpoint protein Bub1 has been found to be mutated at low frequency in certain human cancers characterized by aneuploidy. Simian virus 40 large T antigen efficiently immortalizes rodent cells and occasionally transforms them to tumorigenicity. T antigen can also cause genomic instability, inducing chromosomal aberrations and aneuploidy. Here, we report an interaction between Bub1 and T antigen. T antigen coimmunoprecipitates with endogenous Bub1 and Bub3, another component of the spindle checkpoint complex. Genetic analysis demonstrates that the interaction of T antigen with Bub1 is not required for immortalization but is closely correlated with transformation. T antigen induces an override of the spindle checkpoint dependent on Bub1 binding. This interaction with proteins of the spindle checkpoint machinery suggests another role for T antigen and provides insight into its ability to cause chromosomal aberrations, aneuploidy, and transformation.